Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 858,185 T→A 6.4% T440S (ACA→TCA)  yfkN ← exported 2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase, 2' (or 3') nucleotidase and 5' nucleotidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome858,1850TA 6.4% 204.1 / 4.4 62T440S (ACA→TCA) yfkNexported 2',3'‑cyclic‑nucleotide 2'‑phosphodiesterase, 2' (or 3') nucleotidase and 5' nucleotidase
Reads supporting (aligned to +/- strand):  ref base T (41/17);  new base A (2/2);  total (43/19)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

CTGACATCTCCAGCCAGTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGCGTACCACTTTTGCGCGTCAGTCACGATCTGAA  >  BS168_reference_genome/858065‑858316
                                                                                                                        |                                                                                                                                   
ctgACATCTCCAGCCAGTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAATGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGtt                                                                                                                   >  4:289451/1‑139 (MQ=255)
ctgACATCTCCAGCCAGTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGtt                                                                                                                   >  6:256994/1‑139 (MQ=255)
   aCATCTCCAGCCAGTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGttt                                                                                                                  <  7:315987/137‑1 (MQ=255)
     aTCTCCAGCCAGTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTgc                                                                                                              >  2:382226/1‑139 (MQ=255)
     aTCTCCAGCCAGTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATAGAAAGATCTCCTGCTGGAATGTTTGTATAGTAATTTGCTCAGTTTCTgc                                                                                                              >  6:67719/1‑139 (MQ=255)
          cagccagTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTgccgc                                                                                                           <  5:256994/137‑1 (MQ=255)
          cagccagTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTgccgc                                                                                                           <  8:100718/137‑1 (MQ=255)
          cagccagTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGGTTGTATAGTAATTGGCTCCGTTTCTgcc                                                                                                             <  8:49910/135‑1 (MQ=255)
                 tCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAAtt                                                                                                                            <  6:21677/113‑1 (MQ=255)
                 tCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAAtt                                                                                                                            >  5:21677/1‑113 (MQ=255)
                 tCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTa                                                                                                   >  8:319876/1‑138 (MQ=255)
                 tCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTa                                                                                                   >  7:68967/1‑138 (MQ=255)
                 tCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCATAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTa                                                                                                   >  7:176569/1‑138 (MQ=255)
                      gACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAGACGgc                                                                                             >  7:219564/1‑139 (MQ=255)
                          tCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTaa                                                                                                                              >  1:193875/1‑102 (MQ=255)
                          tCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTaa                                                                                                                              <  2:193875/102‑1 (MQ=255)
                           cGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTc                                                                                         >  6:319287/1‑138 (MQ=255)
                                        cTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATc                                                                           >  2:390147/1‑139 (MQ=255)
                                            aCGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCa                                                                       <  1:382226/139‑1 (MQ=255)
                                              gATTTTAACAGTATTATCGTAAATATACAATTCACCGAAATTTTTAATCGCAAGATATCCGGCTGTAATGTTTGTATCGTAATTGGCTCCTTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAgg                                                                     <  5:67719/139‑1 (MQ=255)
                                                tttGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGtt                                                                   >  6:364288/1‑139 (MQ=255)
                                                  tGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTgccgc                                                                                                           >  8:183831/1‑97 (MQ=255)
                                                  tGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTgccgc                                                                                                           <  7:178390/97‑1 (MQ=255)
                                                  tGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTgccgc                                                                                                           <  7:183831/97‑1 (MQ=255)
                                                  tGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTgccgc                                                                                                           >  8:178390/1‑97 (MQ=255)
                                                  tGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCt                                                                 >  4:198878/1‑139 (MQ=255)
                                                        gTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAGACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACt                                                           <  8:219564/139‑1 (MQ=255)
                                                         tattatCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAgg                                                                     >  5:175202/1‑128 (MQ=255)
                                                         tattatCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAgg                                                                     <  6:175202/128‑1 (MQ=255)
                                                         tattatCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTc                                                          >  1:85483/1‑139 (MQ=255)
                                                         tattatCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTc                                                          <  5:319287/139‑1 (MQ=255)
                                                              tCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCt                                                                                                      <  6:406528/90‑1 (MQ=255)
                                                              tCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCt                                                                                                      >  5:406528/1‑90 (MQ=255)
                                                              tCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAgg                                                                     >  7:243685/1‑123 (MQ=255)
                                                              tCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAgg                                                                     <  8:243685/123‑1 (MQ=255)
                                                              tCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTAt                                                     >  1:56497/1‑139 (MQ=255)
                                                              tCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTAt                                                     >  7:303198/1‑139 (MQ=255)
                                                                  aaaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCtt                                                 >  3:216038/1‑139 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGtata                                                                                                                                  >  1:250911/1‑57 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGtata                                                                                                                                  <  2:250911/57‑1 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTACACTGCGCTCCAGCGGATAAAAACGCCAGGTTCTTGTACTCGGTATCCtt                                                 >  7:2932/1‑138 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACTGCGCTCCAGCGGATAAAATCTGCAGGTTCTTGTACTCGGTATCCtt                                                 >  3:275910/1‑138 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCtt                                                 >  8:30172/1‑138 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCtt                                                 >  7:38907/1‑138 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCtt                                                 >  4:150751/1‑138 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCtt                                                 >  4:207208/1‑138 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTCATTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCtt                                                 >  4:328868/1‑138 (MQ=255)
                                                                   aaGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGATTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCtt                                                 >  6:230092/1‑138 (MQ=255)
                                                                   aaGATACAAGTCACAGACATTTTTAATCGAAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCATGCTTTAAACGGCGCTCCCGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCAtt                                                 >  6:163637/1‑138 (MQ=255)
                                                                        aCAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTc                                           >  2:152487/1‑139 (MQ=255)
                                                                          aaGTCACCGACATTTTTAATCGCAAGATCTCCGTCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCAAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCtt                                         >  8:74794/1‑139 (MQ=255)
                                                                           aGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCttt                                        >  8:303038/1‑139 (MQ=255)
                                                                               aCCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCttttt                                      >  2:327233/1‑137 (MQ=255)
                                                                                ccGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAg                                   >  3:145086/1‑139 (MQ=255)
                                                                                ccGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGAATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAg                                   >  7:135785/1‑139 (MQ=255)
                                                                                ccGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGAATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAg                                   >  7:141790/1‑139 (MQ=255)
                                                                                 cGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGTGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGc                                  >  7:420662/1‑139 (MQ=255)
                                                                                 cGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGc                                  >  4:241160/1‑139 (MQ=255)
                                                                                                  aGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGCTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGCGTACCACTTTTGCGCgt                 <  5:230092/139‑1 (MQ=255)
                                                                                                         cGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGCGTACCACTTTTGCGCGTCAGTCAc          >  7:170346/1‑139 (MQ=255)
                                                                                                            cTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGTGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGCGTACCACTTTTGCGCGTCAGTCACGAt       <  8:420662/139‑1 (MQ=255)
                                                                                                            cTGGAATGTTTGAATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGCGTACCACTTTTGCGCGTCAGTCACGAt       <  8:135785/139‑1 (MQ=255)
                                                                                                            cTGGAATGTTTGAATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGCGTACCACTTTTGCGCGTCAGTCACGAt       <  8:141790/139‑1 (MQ=255)
                                                                                                                 aTGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGCGTACCACTTTTGCGCGTCAGTCACGATCTGaa  >  4:317253/1‑139 (MQ=255)
                                                                                                                        |                                                                                                                                   
CTGACATCTCCAGCCAGTCCTTGACCTCGCTGCCTGTCAGCTTGACGATTTGAACAGTATTATCGTAAAGATACAAGTCACCGACATTTTTAATCGCAAGATCTCCGGCTGGAATGTTTGTATAGTAATTGGCTCCGTTTCTGCCGCCTGCTTTAAACGGCGCTCCAGCGGATAAAATCGGCAGGTTCTTGTACTCGGTATCCTTCATTTCTTTTTCAGCGTACCACTTTTGCGCGTCAGTCACGATCTGAA  >  BS168_reference_genome/858065‑858316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: