| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 1,023,150 | C→A | 34.0% | intergenic (+50/‑200) | yhdF → / → bcaP | putative NAD(P)‑dependent dehydrogenase/branched‑chain amino acid transporter or methylthioribose‑degradation metabolite exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,023,150 | 0 | C | A | 34.0% | 98.8 / 14.2 | 63 | intergenic (+50/‑200) | yhdF/bcaP | putative NAD(P)‑dependent dehydrogenase/branched‑chain amino acid transporter or methylthioribose‑degradation metabolite exporter |
| Reads supporting (aligned to +/- strand): ref base C (11/29); new base A (19/3); total (31/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGAATAATGACCATTG > BS168_reference_genome/1023024‑1023284 | tgctgGCGTCTGACGCATCTTCCTATATGACAGGGCAGACCATTAATGTGTATGGCGGCCGTGTTTTTTTAACGTTAAAAACGAAAAAACAACTCAAAAGCTTGGTTTTTTTTT‑TTGGTAAAAATTaaaaaaaaaaaat > 8:239888/1‑138 (MQ=255)tgctgGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTGTTTTTTTAAAGTAAACAAAGAAAAACCCCCTCAAGACC‑TGGGGTTTTTTG‑TGGGGTAAAATATAAAAATaaaaaa > 5:147283/1‑138 (MQ=255)tgctgGCGTCTGACGAATCTTCCGATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTGTTTTTTGAACGTAATCAAACAAAAACCCCCTCAACACC‑GGGGTTTTTTTG‑TGGTGTAAAAATtaaaaataaaaatt > 7:124798/1‑137 (MQ=255) ctgGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACCGAATCAACCAAAAACCCCCACAAGCGC‑GGGGTTTTTTTG‑TGGTGTAAAATTTAAAAAAAAAAAAatt > 5:197509/1‑139 (MQ=255) ctgGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTAAACGTAATAAACCAAAAAACAACGCAAGAGC‑AGGTTTTTTGTG‑TGGTGAAAAATTTAAAAATAAAAAAatt > 5:178835/1‑139 (MQ=255) ctgGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTGTTTTTTAAACGTAATAAAACAAAAAAAACCACAAAAGC‑TGGTTTTTTTTGTTGTTGAAAAATTTAAAAAAAAAAAAtt > 2:53830/1‑139 (MQ=255) tCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTAAAAGTAATAAACGAAAAACAAACTCAAGACC‑TGGTTTTTTTTG‑TGGTGAAAAAATTAAAAAAAAAAAATTTTTAG‑ATTTTTTTat > 7:11481/1‑137 (MQ=255) cTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TAGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑tt < 3:193511/138‑1 (MQ=255) atatGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTAAAAGTAATCAACCAAAAAAAACCTCAAGAGC‑GGGTTTTTTTTG‑TGGTGTAAAATTTCAAAATAAAAAATTTTTAG‑ATTTTTGTTACAA‑ATTTg > 5:303930/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTTTTTCAACGTAATCAAAGAAAAACCAGCTCAAGAGC‑TGGTTTTTTTTG‑TGGTGAAAAAATTAAAAATAAAAAATTTTTAG‑ATTTTTATAACAA‑TTTTTTTTTAAttt > 5:82727/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTTTTTCAACCGAAACAACCAAAAAAAAACACAAGAGA‑AGGTGTTTTGTG‑TGTTGTAAAATTTAAAAAATAAAAATATTTAG‑AATTTTTTAACAATTTTTGTTTTAAtt > 8:347488/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTTTTTAAAAGTAATAAAAGAAAAAACACCACAAGAGC‑TGGTTTTTTTTT‑TGGTGAAAAATTTAAAAAAAAAAAAATTTTAG‑ATTTTTTTTACAA‑TTTTTTTTTTAttt > 6:280660/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTATTTGAACGTAATAAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTAAAAATAAAAAATTTGTCG‑ATTTTTGTATAAA‑TTTTTTTTTGAttt > 7:79321/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTATTTGAACGTAATAAACGAAAAAACAGCTCAAGAGC‑GGGTTTTTTTTG‑TGGTGTAAAATTTAAAAATAAAAAATTTTTAG‑ATTTTTATAACAA‑TTTTTTTTTTAttt > 7:369091/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATAAACGAAAAAACACCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTAAAAATAAAAAATTTGTAG‑ATTTTTATAACAA‑ATTTTTTTTAAttt > 8:417683/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTTTTATTTAAACCTAATCAACCAAAAACCACCTCAAGAGC‑TGGTTTTTTTTG‑TGGTGAAAAAATTAAAAATAAAAAAATTTTAG‑ATTTTTTTAACAA‑ATTTTTTTTAAttt > 6:329003/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTGTTTTTTGAACGTTATCAACGAAAAACCACCGCAAGAGC‑TGGTTTTTTGTT‑TGGTGTAAAATTTAAAAATAAAAAAATTGTGG‑ATTTTTATTAAAA‑ATTTTTTTTTAttt > 7:181616/1‑139 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTGTTTTTTGAACGGAAATAACCAAAAAACAACAGAACAGC‑GGGTTTTTTTTT‑TGGTGGAAAAATTAAAAATAAAAAAATTTTTG‑ATTTTTGTTATAA‑Ttttttttttgttt > 4:269567/1‑134 (MQ=255) ggCAGACCATTCATGTGAATGGCGGCCGTGTTATTTAAACGTAATAAAAGAAAAAACAGCTCAAGGGC‑GGGGTTTTTGTG‑TGGGGTAAAATTTAAAAAAAAAAAATTTGTGG‑ATTTTTATAAAAA‑ATTTTTTTTTAttt > 5:373758/1‑139 (MQ=255) cAGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCAttttt > 7:417436/1‑139 (MQ=255) aGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTg < 3:1194/139‑1 (MQ=255) aCCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCACCTCAAGAGC‑TGGGTTTTTGTG‑TGGTGAAAAATTTAAAAATAAAAAATTTTTCG‑ATTTTTATAAAAA‑TTTTGTTTTAAttttttg > 1:1346/1‑136 (MQ=255) aCCATTCATGTGAATGGCGGCCGTGTTATTTAAAAGTAATAAAAGAAAAAACAGCGCAAGAGC‑TGGGTTTTTTTG‑TTGTGTAAAATTTAAAAAAAAAAAAATTTTGG‑ATTTTTTTTAAAA‑ATTTTTTTTTAttttttg > 4:416361/1‑136 (MQ=255) aCCATTCATGAGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCa < 5:276252/139‑1 (MQ=255) aTTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAAtt < 4:82927/139‑1 (MQ=255) tCATGTGAATGGCGGCCGTTTTATTTAAACCTAATAAACCAAAAACCCCCACAAGCGC‑GGGTGTTTTTGG‑TGGGGAAAAATTTCAAAAAAAAAAAATTTTGG‑CTTTTTATAACAA‑ATTTTTTTTTATTTTTGCAAtttt > 6:362581/1‑139 (MQ=255) tCATGTGAATGGCGGCCGTGTTTTTTTAAAGTTATAAAAGAAAAAACCACACAAGAGC‑GGGGTTTTTTGG‑TGGTTTTAAAATTTAAAATAAAAAAATTGTGGTATTTTTTTAA‑AA‑TTTTTTTTTTATTTTTTGAAtttt > 7:164463/1‑139 (MQ=255) cATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAAtttt < 2:227635/138‑1 (MQ=255) cATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAAtttt < 5:280660/138‑1 (MQ=255) cATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGGTTTCATTTTTGCAAtttt < 5:380500/138‑1 (MQ=255) aTGTGACTGGCGGACGTTTTATTTCAACGTAATCAACGAAAAACCCGCTCAAGAGC‑TGGTTTTTTGTG‑TTGTGAAAAATTTAAAAATAAAAAATTTGTCG‑ATGTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTtat < 7:347488/139‑1 (MQ=255) aTGTGAATGGCGGCCGTTTTATTTAAACTTAATAAACTAAAAACATTCTCACTAGA‑TGGTTTTTTTTT‑TGGTTAAAAATTGAAAAAT‑AAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTtat < 8:124798/138‑1 (MQ=255) tgAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTtatata < 1:354102/139‑1 (MQ=255) tgAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTtatata < 8:181616/139‑1 (MQ=255) tgAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTtatata < 7:239888/139‑1 (MQ=255) tgAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTtatata < 3:416361/139‑1 (MQ=255) aaTGGCGGCCGTTTTATTTGAAAGTAATCAACGAAAAAACACCTCAAGAGC‑GGGGTTTTTGTG‑TGGGGAAAAAATTAAAAAAAAAAAATTTTTCG‑ATTTTTTTTAAAA‑TTTTTTTTTAATTTTTTTAATTTtatatat > 3:82927/1‑138 (MQ=255) aaTGGCGGCCGTGTTATTTGAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGGTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTTACAA‑TTTTGTTTTCATTTTTGCAATTTtattttt > 7:116236/1‑134 (MQ=255) tGGCGGCCGTTTTATTTAAACGTAATAAACCAAAAAACAACGCAATAGC‑TGGGTTTTTTTG‑TGGTGTAAAATTTAAAAATAAAAAATTTTTTG‑ATTTTTATAAAAA‑ATTTTTTTTATTTTTTTGAATTTtttaaatta > 6:102443/1‑136 (MQ=255) gcggcCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATataata < 7:96618/138‑1 (MQ=255) gcggcCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATataata < 7:417683/138‑1 (MQ=255) gcggcCGTTTTATTTCAAAGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAAATTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATCTataata < 6:186630/138‑1 (MQ=255) cggcCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATAtt < 5:285473/139‑1 (MQ=255) ccGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAg < 3:272134/139‑1 (MQ=255) ttttATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAg < 8:417436/139‑1 (MQ=255) cGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAAt > 1:155231/1‑139 (MQ=255) aTCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATataata > 1:143885/1‑116 (MQ=255) aTCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATataata < 2:143885/116‑1 (MQ=255) accaaaaaCCAGCTCAAGAGC‑TGTTTTTTTGTG‑TGGTTAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCAAACAA‑TTTTGTTTTCATTTTTGCAATTTTAAATATAATATTGAGTAGGAGAAAGTAATGATTcgcg < 6:373758/136‑1 (MQ=255) cGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGATTGATTCGCGa > 8:396507/1‑139 (MQ=255) gAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTTAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTATAACAA‑TTTTTTTTTCATTTTTGCATTTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGAc < 4:98765/139‑1 (MQ=255) gAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGAc < 5:92757/139‑1 (MQ=255) aaaaaCCCGCGCAAGCGC‑TGGTTTTTTGTG‑TGGTTAAAAATTACAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAACGGAATGATTCGCGAcc < 5:362581/139‑1 (MQ=255) aaaaaCCAGCTCAAGAGC‑TGGTTTTTTGTG‑TTGTTAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAAAAA‑TTTTGTTTTAATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGAcc < 3:269567/139‑1 (MQ=255) aaaaaCCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGAcc < 6:303930/139‑1 (MQ=255) aaaaaCCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTAATAAATAATATTGAGTAGGAGAAAGGAATGATTCGCGAcc < 3:81912/139‑1 (MQ=255) aaaaaCCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTAATTTTTGCAATTTTATATATAATATTGAGTAGGATAAAGGAATGATTCGCGAcc < 8:369091/139‑1 (MQ=255) aaaaaCCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTATAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGAcc < 1:53830/139‑1 (MQ=255) aaaaCCAGATCAATAGC‑TGGTTTTTTGTG‑TGGTTAAAAATTTCAAAATAAAAAATTTTGCG‑TTTTTTCAAACAT‑TTTTTTTTTCATTTTTGAAATTTTATATATAATATTGAGTAGGAGAAAGGAAGTATTCGCGAcc < 6:147283/138‑1 (MQ=255) aaaCCAGCTAAAGAGG‑TGGTTTTTTGTG‑TGTTTAAAAATTTAAAAATAAAAAATTTGTCT‑ATTTTTCTAAAAT‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGATAAAGGAATTATTCGCGACCAg < 8:11481/139‑1 (MQ=255) gCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACaaa > 1:38734/1‑139 (MQ=255) ggTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGaa > 5:111324/1‑138 (MQ=255) tggtgAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGCCCAGTACAAATATAGTAGAAAAAAAAGaataa > 5:422027/1‑139 (MQ=255) tggtgAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGaataa > 4:301202/1‑139 (MQ=255) aTTTCAAAATAAAAAATTTGTCT‑ATTTTTCTAACAA‑TTTTGTTTTAATTTTTCCAATTTTATATATAATATTGAGTAGGAGAACGTTTTGATCCGCGACCAGTACAAATATAGTAGAAAAAAAAGAATAATGACCATTg < 5:102443/139‑1 (MQ=255) | TGCTGGCGTCTGACGAATCTTCCTATATGACAGGGCAGACCATTCATGTGAATGGCGGCCGTTTTATTTCAACGTAATCAACGAAAAACCAGCTCAAGAGC‑TGGTTTTTTGTG‑TGGTGAAAAATTTCAAAATAAAAAATTTGTCG‑ATTTTTCTAACAA‑TTTTGTTTTCATTTTTGCAATTTTATATATAATATTGAGTAGGAGAAAGGAATGATTCGCGACCAGTACAAATATAGTAGAAAAAAAAGAATAATGACCATTG > BS168_reference_genome/1023024‑1023284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |