Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,142,219 T→G 17.5% F805V (TTT→GTT)  addA → ATP‑dependent deoxyribonuclease (subunit A)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,142,2190TG17.5% 75.7 / 4.7 42F805V (TTT→GTT) addAATP‑dependent deoxyribonuclease (subunit A)
Reads supporting (aligned to +/- strand):  ref base T (13/21);  new base G (6/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGACACTTCC  >  BS168_reference_genome/1142087‑1142346
                                                                                                                                    |                                                                                                                               
ttCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAg                                                                                                                           <  6:215123/139‑1 (MQ=255)
  ccTGCTGTTTATCGAACGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGc                                                                                                                          <  5:82195/138‑1 (MQ=255)
  ccTGCGGTTTATCGCCCGCATGCAGGAGCGGGGCGCTGATCTTGGTACGGCGAGCGCGCTCAGCGCGCAGGAGGCTGTGGGCCGCTTAATGACGATCCAGCGCTGCAAAGGGCTCGAATTTCCAGTCGTGTTTGttg                                                                                                                           >  7:338963/1‑135 (MQ=255)
                     aTGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTTTCAGGGATCGGCCGGAttttt                                                                                                       >  2:257484/1‑137 (MQ=255)
                           gAGCGGGGCGGTGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACatga                                                                                                <  2:122833/139‑1 (MQ=255)
                              cGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTTGCAGGTCTAGGGGGGCATTTGAACATGATgg                                                                                             >  7:162423/1‑139 (MQ=255)
                                 ggCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGCCGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTGTGTAGCAGGTGTCGGCCGGAATTTCAACATGATGGAtt                                                                                          >  7:325170/1‑139 (MQ=255)
                                     atgatCTTGGTAGGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTTTAGCAGGTGTCGGCCGGAATTTCAACAAGATGGATTTGa                                                                                       >  2:263521/1‑138 (MQ=255)
                                     atgatCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGaa                                                                                      <  3:89743/139‑1 (MQ=255)
                                        atCTTGGGACGGCGAGAGCGCTCAGCGAGCAGGAGGCTGTTGGCCGCTTACTGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAAc                                                                                     <  5:63495/137‑1 (MQ=255)
                                             ggTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGt                                                                              <  2:130769/139‑1 (MQ=255)
                                              gTACGGCGAGAGCGCTCAGCGAGCAGGAGGATTTTGTCCTCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCTGCCTGAATTTCAACATGATTGATTTGAACAAATCGt                                                                              <  8:318610/138‑1 (MQ=255)
                                                    cGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTTGCAGGTCTCGGCCGGAATTTAAACATGATGGATTTGAACCAATCGTTCctggt                                                                       >  8:208685/1‑137 (MQ=255)
                                                    cGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACctgct                                                                       >  4:389910/1‑139 (MQ=255)
                                                     gagaGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCACAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGGATCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACctgctg                                                                      <  1:343620/139‑1 (MQ=255)
                                                        agCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTTGCAGGTCGCGGCCGGAATTTAAACATTATGGTATTGAACAAATCGTTCCAGCGGGAt                                                                   >  4:135877/1‑139 (MQ=255)
                                                                           gaTGTTGTCCGCTTACTGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                                <  4:108743/139‑1 (MQ=255)
                                                                           gaTGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                                <  3:370788/139‑1 (MQ=255)
                                                                           gaTGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                                <  3:67904/139‑1 (MQ=255)
                                                                           gaTGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                                <  1:271346/139‑1 (MQ=255)
                                                                           gaTGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                                <  1:224944/139‑1 (MQ=255)
                                                                           gaTGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                                <  7:244162/139‑1 (MQ=255)
                                                                           gaTGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGGCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                                <  3:281376/139‑1 (MQ=255)
                                                                            aTGTTGTCCGCTTAATGACGATCCACAGCAGCATAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCAc                                               <  3:233741/139‑1 (MQ=255)
                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTTTAGCAGGTCTCGGCCGGAATTTCAACATGTTGGAGTTGTACAAAAAGTACCTGCGGGCTTAGGAGCGCGGATTTGGCACg                                              >  6:200623/1‑139 (MQ=255)
                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACg                                              <  3:389910/139‑1 (MQ=255)
                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACg                                              <  7:98532/139‑1 (MQ=255)
                                                                             tgttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACg                                              <  3:112512/139‑1 (MQ=255)
                                                                               ttgtCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGTTGTTGCAGGGGTGGGCCGGGATTTGCACATGATGGATTTGTACAAAAAGTACCTGCGGGTGAATGAGCCCGCAATTggggcga                                             >  3:367051/1‑133 (MQ=255)
                                                                                  tCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTTGCACGTGTCGGCCGGAATTTCAAAATAATGGATTTGTACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTTGCACGAAGta                                         >  7:123200/1‑139 (MQ=255)
                                                                                  tCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCCGGTCGCCGCCGGGATTTCAACATGATGGGTTTGAACAAATCGTACCGGCAGGCTTAGGAGGTCGGGTTTGGGACgtagta                                         >  7:341838/1‑139 (MQ=255)
                                                                                  tCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                                <  2:82867/132‑1 (MQ=255)
                                                                                  tCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCa                                                >  1:82867/1‑132 (MQ=255)
                                                                                      cTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGGGTGTTGCAGGTGTCGGGCGGCATTTGAACAATATTGTTTTTAAAAAATCGTACCAGCTGGATAAGGATCTCGGTTTTTGCACGTAGTatat                                      >  6:45228/1‑138 (MQ=255)
                                                                                                           caAAGGGCTCGAATTTCCAGTCGTGTTTGTTGCAGGTCTCGGCCGGAATTTGAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGc                >  5:170665/1‑139 (MQ=255)
                                                                                                           caAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTAAACATGATGGATTTGAACAAATAGTACCTGCTGGATAAGGAGCTCGGCTTTGGGACGAAGTATATTAATCCGCAATTACGCAACAg                 >  6:139536/1‑138 (MQ=255)
                                                                                                           caAAGGGCTCGAATTTCCAGTCGTGGTTGTTGCAGGTATCGGGCGGCATTTCAACAACATGGATTTTAACAAATCGTACATGGGGGTTAAGGAGGGCGGATTTTGCACGAAGTATATTCAAACCCAATTAAGCaaact                 >  4:296299/1‑134 (MQ=255)
                                                                                                                ggCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATcc           <  1:263521/139‑1 (MQ=255)
                                                                                                                  cTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGa         <  3:135877/139‑1 (MQ=255)
                                                                                                                          tCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGa         <  7:261548/131‑1 (MQ=255)
                                                                                                                          tCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGa         >  8:261548/1‑131 (MQ=255)
                                                                                                                           ccAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATTATTGATTTGAACAAATCGTACCTGCTGGATAATGAGCTAGGATTTGGCCCGAAGTATATTAAAACGCAATTAAGCATCAGCTATACGACACtaac  >  2:354495/1‑134 (MQ=255)
                                                                                                                                    |                                                                                                                               
TTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATGATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAATTTCCAGTCGTGTTTGTAGCAGGTCTCGGCCGGAATTTCAACATGATGGATTTGAACAAATCGTACCTGCTGGATAAGGAGCTCGGATTTGGCACGAAGTATATTCATCCGCAATTACGCATCAGCTATCCGACACTTCC  >  BS168_reference_genome/1142087‑1142346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑