Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,690,758 C→G 87.7% R214G (CGT→GGT)  codY → transcriptional regulator, GTP and BCAA‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,690,7580CG87.7% 119.5 / 5.7 44R214G (CGT→GGT) codYtranscriptional regulator, GTP and BCAA‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/2);  major base G (20/17);  minor base A (3/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

AAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGA  >  BS168_reference_genome/1690623‑1690879
                                                                                                                                       |                                                                                                                         
aaaGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGtt                                                                                                                        <  1:290997/139‑1 (MQ=255)
aaaGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATGTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGtt                                                                                                                        <  2:157651/139‑1 (MQ=255)
  aGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCt                                                                                                                      >  7:250734/1‑139 (MQ=255)
  aGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCt                                                                                                                      >  3:21300/1‑139 (MQ=255)
       tCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTAt                                                                                                                 >  3:331007/1‑139 (MQ=255)
             aaaTGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGaa                                                                                                           <  2:146671/139‑1 (MQ=255)
                      tCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCa                                                                                                   <  4:331648/138‑1 (MQ=255)
                      tCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCa                                                                                                   <  8:364694/138‑1 (MQ=255)
                      tCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCa                                                                                                   <  4:331007/138‑1 (MQ=255)
                                ttCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGga                                                                                         <  2:421913/138‑1 (MQ=255)
                                      cAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                   <  7:253086/138‑1 (MQ=255)
                                        gTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCg                                                                                 >  5:156914/1‑138 (MQ=255)
                                        gTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCg                                                                                 <  6:156914/138‑1 (MQ=255)
                                         tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCa                                                                                                   <  6:42044/119‑1 (MQ=255)
                                         tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCa                                                                                                   >  5:42044/1‑119 (MQ=255)
                                         tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt                                                                               >  3:90219/1‑139 (MQ=255)
                                         tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt                                                                               >  7:76818/1‑139 (MQ=255)
                                          gAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtg                                                                              >  1:326811/1‑139 (MQ=255)
                                           aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                   <  7:144468/133‑1 (MQ=255)
                                           aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                   <  6:75477/133‑1 (MQ=255)
                                           aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                   >  8:144468/1‑133 (MQ=255)
                                           aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc                                                                                   >  5:75477/1‑133 (MQ=255)
                                           aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                                                             >  2:106541/1‑139 (MQ=255)
                                           aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt                                                                             >  3:378790/1‑139 (MQ=255)
                                             cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCa                                                                                                   >  2:140640/1‑115 (MQ=255)
                                             cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCa                                                                                                   <  1:140640/115‑1 (MQ=255)
                                                gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                         <  4:21300/138‑1 (MQ=255)
                                                gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc                                                                         <  8:76818/138‑1 (MQ=255)
                                                     aaTTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCt                                                                   <  3:163166/139‑1 (MQ=255)
                                                       ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGCGCGCCGGTGTTATCGAGTc                                                                    >  4:163166/1‑136 (MQ=255)
                                                          aGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGAt                                                               >  8:129929/1‑138 (MQ=255)
                                                                      aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                  <  4:90219/139‑1 (MQ=255)
                                                                      aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa                                                  <  4:378790/139‑1 (MQ=255)
                                                                      aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGa                                                   >  3:254370/1‑138 (MQ=255)
                                                                         agCTTGACGGAAATGAAGGACTTCTTCTTGCAAGAAAAATAGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAgg                                               >  2:356347/1‑139 (MQ=255)
                                                                             tGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACt                                           >  3:411788/1‑139 (MQ=255)
                                                                                    aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa                                    <  1:339149/139‑1 (MQ=255)
                                                                                     aTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATc                                     >  3:164644/1‑137 (MQ=255)
                                                                                     aTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCAGTTCTGTTATTGTGAACGAACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATc                                     >  4:96830/1‑137 (MQ=255)
                                                                                                    ttgCAAGTAAAATTGCTGACCGTGGCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAAcaacaa                    <  8:140358/139‑1 (MQ=255)
                                                                                                        aaGTAAAATTGCTGACCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTAGCGAGTCTAGATCATTAGGATGGAATGGTACTTATATCAAGGTACTAAACAACAAATTc                >  7:401241/1‑139 (MQ=255)
                                                                                                                     gACCGTGTCGGCATTACCGGGTCTGTTATTTTGAACGCACTCTGAAAGCTGTAGAGAGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAATGTACTAAACAACAAATTCCTAATTGAAt      >  3:16711/1‑136 (MQ=255)
                                                                                                                      aCCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAg   >  4:379585/1‑138 (MQ=255)
                                                                                                                      aCCGTGTCGGCATTACCGGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGa  <  8:250734/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                         
AAAGCTGTCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGA  >  BS168_reference_genome/1690623‑1690879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑