| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,115,511 | G→T | 25.9% | H387N (CAT→AAT) | cwlS ← | peptidoglycan hydrolase (cell wall‑binding d,l‑endopeptidase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,115,511 | 0 | G | T | 25.9% | 64.8 / 4.2 | 38 | H387N (CAT→AAT) | cwlS | peptidoglycan hydrolase (cell wall‑binding d,l‑endopeptidase) |
| Reads supporting (aligned to +/- strand): ref base G (16/11); new base T (2/9); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTGGTATAAACAAAAAAGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCATGACATTCCAATACCCTGCCGTACTC > BS168_reference_genome/2115375‑2115641 | gTCTGGTATAAACAAAAAAGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGATTTGCTGAGATTGGAAATGTCGACGCCAGATGAGATTGAAGGaa > 1:180658/1‑139 (MQ=255)gTCTGGTATAAACAAAAAAGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATTaa > 7:384940/1‑139 (MQ=255) tCTGGTATAAACAAAAAAGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAAt < 3:399356/139‑1 (MQ=255) gTATAAACAAAAAAGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATaaaa > 5:177769/1‑139 (MQ=255) tataAACAAAAAAGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAAt > 1:48923/1‑139 (MQ=255) aaaaaaaTTCAATAAAATGGTTTTTTTTAATAGTCTTTTTTAAAATAAACTTCTGCCCCCCAGGTACCGGTGTTTCAATTACTATTTGCTTATATTGTAAATGTCGACGCCCGATTAGCTTGCTTTAATAAAATCAccg < 8:103686/138‑1 (MQ=255) aaaaGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACcgccgc > 6:378457/1‑139 (MQ=255) aaaaGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACcgccgc > 6:219699/1‑139 (MQ=255) aaaaGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACcgccgc < 5:378457/139‑1 (MQ=255) aaaaGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGAGGAGCTTGCATGAATAAAATCACcgccgc > 2:191863/1‑139 (MQ=255) ccTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGATATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAg > 3:240319/1‑138 (MQ=255) aa‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATTAATAAAATCACCGCCGCCTAGATAAATcc < 8:384940/139‑1 (MQ=255) aa‑GGCTTTTTGTCATACTAATTTTTAAAAATAACTTCTTGCGCCCATGTCCCGCTGTTACCAATACGAATTGGTGTGATTGTAATTGGCGCCGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATcc < 1:342913/139‑1 (MQ=255) ccggttttttGACATAATAATTTTAAAAAAAAACTTCTGGCCCCCAAGACCCGCTGTTAAAAAAACTAATTGCTGTGTTGGTAATTGCCGACGCCCTATGAGCTTGCATTAATAAAAACACCGCCGCCTAGATAAATcc < 4:240319/134‑1 (MQ=255) gCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCa > 8:301327/1‑139 (MQ=255) gCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGATGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATTAATAAAATCACCGCCGCATAGATAAATcacc > 8:307134/1‑138 (MQ=255) gTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTgag < 2:48923/138‑1 (MQ=255) aCTCATTTTTAAAAATAATTTCTTGCCCCAAAGACCCGCTGTTTCAAATACGAATTGCTGAGATTGCAAATGGCGACGCCCGATGAGCTTGCATGAATAAAAACACCGCCGCCTAGATAAATCCCCATGTGAGACGGGc < 3:89610/139‑1 (MQ=255) aaaaaaaaCTTCTTGCGCCCACCGACCGCTGTTTCCAAAACGAATTGCTGACATTGCAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTtata < 1:354550/139‑1 (MQ=255) aaaaTAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACTAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTtata < 6:177769/138‑1 (MQ=255) cttGCGCCCAAGTACGGCTGTTTCCAATACGAATTGCTGAGATTGTAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAaag < 7:128277/138‑1 (MQ=255) aactACCGCTGTTTCAAATAGGAATGGCTGAGATTGCAAATGGCGACGCCCGATGAGCTTGCATTAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGt < 7:134442/136‑1 (MQ=255) aGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGAGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGtc > 1:345716/1‑139 (MQ=255) aaTACGAATTGGTGAGATTGGAAATGTCGACGCCCGATGAGCTGGCATGAATAAAATCACCGGCGCCGAGATTAATACCCATGTGAGCCGGGCCGGGTTTATATGTGGTTAAGAAGACAAAGTCTCCTAAGGACggctg > 6:269524/1‑139 (MQ=255) aTTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACt > 3:405731/1‑139 (MQ=255) aTTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACt > 3:369233/1‑139 (MQ=255) aTTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACt > 7:113522/1‑139 (MQ=255) gatgcgAAATGCCCACGCCCGCTCAGCTTGCATTAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCAt < 1:403910/134‑1 (MQ=38) aTTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCAt > 5:77854/1‑138 (MQ=255) aTTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCAt > 5:84047/1‑138 (MQ=255) ttGTAAATGACCACGCCCGATGAGCTTGAATTAAAAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCATGa < 7:301327/139‑1 (MQ=255) tccaaaTGTCTCCGCCCGATGAGCTTCCATTAAAAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCATGa < 7:307134/135‑1 (MQ=255) ggcgacgaCCGATGTGCTTGCATGAATAAAATCACCTCCGCCTAGATATATCCCAATGTGAGACGGGACTGATTTATATGTGGTAAAGAAGACAAATTCTCCTACTGACGTCTGGCTGACTTTCTGCATGACATTCCa < 8:230441/136‑1 (MQ=255) ccaCGCCCTATCAGATTAAATTAAAAAACCCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCATGACATTCCAATa < 5:269524/137‑1 (MQ=37) cGACGCCCGAGGAGCTTGCATGAATAAAATCACCGCCTCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCATGACATTCCAATa < 8:4037/139‑1 (MQ=255) aCGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCATGACATTCCAATAcc < 5:219699/139‑1 (MQ=255) cccGATGAGCTTGCATGAATAAAATCACCGCCGCATAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAACTCTCCTACTGCCGGCTGGCTGACTTTCTGCATGACATTCCAATACCCTg > 6:283598/1‑139 (MQ=255) gCTTGCATTATTAAATACACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCATGACATTCCAATACCCTGCCGTACTc < 2:345716/139‑1 (MQ=255) | GTCTGGTATAAACAAAAAAGCCTATAAAA‑GGCTTTTTGTCATACTCATTTTTAAAAATAACTTCTTGCGCCCAAGTACCGCTGTTTCCAATACGAATTGCTGAGATTGGAAATGTCGACGCCCGATGAGCTTGCATGAATAAAATCACCGCCGCCTAGATAAATCCCCATGTGAGACGGGCCGGATTTATATGTGGTAAAGAAGACAAAGTCTCCTACTGACGGCTGGCTGACTTTCTGCATGACATTCCAATACCCTGCCGTACTC > BS168_reference_genome/2115375‑2115641 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |