| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,286,175 | C→T | 6.3% | Q469* (CAA→TAA) | sprA → | serine‑type phage integrase; phage SPbeta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,286,175 | 0 | C | T | 6.3% | 208.5 / 3.1 | 63 | Q469* (CAA→TAA) | sprA | serine‑type phage integrase; phage SPbeta |
| Reads supporting (aligned to +/- strand): ref base C (22/37); new base T (2/2); total (24/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01 | |||||||||||
GAGAACTTCATCTTCGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAGCTATTAAGGATGGTCTTAAAAGATGTTATTGT > BS168_reference_genome/2286040‑2286299 | gagaACTTCATCTTCGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAAt < 1:268290/139‑1 (MQ=255)gagaACTTCATCTTCGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAAt < 1:412169/139‑1 (MQ=255)gagaACTTCATCTTCGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAAt < 1:65440/139‑1 (MQ=255)gagaACTTCATCTTCGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAAt < 7:244956/139‑1 (MQ=255) agaACTTCATCTTCGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAAt > 7:34154/1‑138 (MQ=255) cTTCATCTTCGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGa > 4:425247/1‑138 (MQ=255) ttCATCTTCGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGaaa > 4:299833/1‑139 (MQ=255) cGATATATATGAATGTGGCATTTACCGTGATGAACTTTTTTTGAAGAGTAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACa < 1:426431/137‑1 (MQ=255) cGATAAATATGAAAGTGTCATTTACAGTGATGAACTTTTTTTTAAGAGAAAAGCTGCATTAGAAGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGa < 8:222784/135‑1 (MQ=255) cGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAACGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGa < 8:222788/135‑1 (MQ=255) agATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGa < 3:295456/134‑1 (MQ=255) tAAATATGAAAGTGGCATGTACAGTTATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTa < 2:361080/137‑1 (MQ=255) aaTATGAAAGTGGCATTTACTGTGATTTACTTTTTTTGAAGAGAATAGCTGCATTAGATGAAGATTTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATa < 2:145200/138‑1 (MQ=255) tataaGTGTCATTTACAGTGATGAACTTTTTTTGAAGAGAAACGCTCCATTAGATGAAGACTATAAAGATCTCCAGAATGCAAAGAATGAGCTCAATGTATTACAAGATACACAATCAGAAATAGACATTAATACAGt < 4:260178/135‑1 (MQ=255) tGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATa < 7:181864/118‑1 (MQ=255) tGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATa > 6:237329/1‑118 (MQ=255) tGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATa > 8:181864/1‑118 (MQ=255) tGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATa < 5:237329/118‑1 (MQ=255) gCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGaaa < 7:11741/114‑1 (MQ=255) gCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGaaa > 8:11741/1‑114 (MQ=255) cATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAaacaa > 6:70618/1‑138 (MQ=255) tACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACata > 4:298223/1‑138 (MQ=255) aCAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGa > 4:144786/1‑112 (MQ=255) aCAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGa < 3:144786/112‑1 (MQ=255) aGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACataaat > 3:5505/1‑138 (MQ=255) aGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATAAACAATCAGAAATAGACAGTAATACAGTGAGAAACAACataaat > 7:167662/1‑138 (MQ=255) tgatgaACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACATAATCAGAAATAGACAGTAATACAGTGAGAAACAACataaataa > 5:320262/1‑138 (MQ=255) tgatgaACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACATAATCAGAAATAGACAGTAATACAGTGAGAAACAACataaataa > 5:314359/1‑138 (MQ=255) tgatgaACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATa > 6:23492/1‑106 (MQ=255) tgatgaACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATa < 5:23492/106‑1 (MQ=255) atgaACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGa < 8:151727/106‑1 (MQ=255) atgaACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGa > 7:151727/1‑106 (MQ=255) atgaACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATaaataaaa > 7:22645/1‑138 (MQ=255) atgaACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATaaataaaa > 3:131614/1‑138 (MQ=255) atgaACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATaaataaaa > 5:24198/1‑138 (MQ=255) tttttGCAGAGAACAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc < 4:226624/138‑1 (MQ=255) tttttGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACATAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc < 6:314359/138‑1 (MQ=255) tttttGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACATAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc < 6:320262/138‑1 (MQ=255) tttttGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc > 5:422842/1‑138 (MQ=255) tttttGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc < 3:425247/138‑1 (MQ=255) tttttGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc < 3:298223/138‑1 (MQ=255) tttttGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc < 8:34154/138‑1 (MQ=255) tttttGAAGAGAAAAGCTGCATTAGATGAAGAATATAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc < 1:131387/138‑1 (MQ=255) tttttGAAGAGAAAAGCTGCATTAGATGAAGAAGTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc < 1:370389/138‑1 (MQ=255) tatttGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAAAGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGAc < 7:375835/136‑1 (MQ=255) ttAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCt < 3:299833/139‑1 (MQ=255) agaaTTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACAtt < 4:295716/121‑1 (MQ=255) agaaTTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACAtt > 3:295716/1‑121 (MQ=255) agaaTTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGaa > 1:180700/1‑138 (MQ=255) aTTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGaaa > 3:21053/1‑136 (MQ=255) aaGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACg < 7:184457/138‑1 (MQ=255) aaGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACg < 6:24198/138‑1 (MQ=255) aaGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACg < 3:400635/138‑1 (MQ=255) agagCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAg < 7:226599/139‑1 (MQ=255) agagCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAg < 8:112050/139‑1 (MQ=255) agagCTCCAGAATGCAAAGAATGAGCTCAATGGACTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAg < 2:169272/139‑1 (MQ=255) atcaaaaTAATGACCTCAATGGCTTACCAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATATAATAATTGACCAGTAGCACATTGAATCAAGCTCAGAGAAAAAGAACGAGCTATTAAGGAt < 2:292049/135‑1 (MQ=255) aTGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAGCTATTAAGGAt < 5:70618/139‑1 (MQ=255) aaTGGATTACAAGATACACAATCAGAAATAGACTGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAGCTATTAAGGATGGTCTTAAAAGATGTTa < 4:5505/139‑1 (MQ=255) aaTGGATTACAAGATACACAATAATAAATAGACAGTAATACAGTTAGAAACAACATAAATAAAATTATTGAACAGTATCACATTGAATCAAGATCAGAGAAAAAGAACGAGCTATTAAGGATGGTCTTAAAAGATGTTa < 8:167662/139‑1 (MQ=255) aTTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAGCTATTAAGGATGGTCTTAAAAGATGTTATTGt > 4:319162/1‑138 (MQ=255) tACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAGCTAtt > 2:313966/1‑102 (MQ=255) tACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAGCTAtt < 1:313966/102‑1 (MQ=255) | GAGAACTTCATCTTCGATAAATATGAAAGTGGCATTTACAGTGATGAACTTTTTTTGAAGAGAAAAGCTGCATTAGATGAAGAATTTAAAGAGCTCCAGAATGCAAAGAATGAGCTCAATGGATTACAAGATACACAATCAGAAATAGACAGTAATACAGTGAGAAACAACATAAATAAAATAATTGACCAGTATCACATTGAATCAAGCTCAGAGAAAAAGAACGAGCTATTAAGGATGGTCTTAAAAGATGTTATTGT > BS168_reference_genome/2286040‑2286299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |