Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 2,369,193 G→A 7.7% P16P (CCC→CCT)  aroA ← 3‑phosphoshikimate 1‑carboxyvinyltransferase (5‑enolpyruvoylshikimate‑3‑phosphate synthase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome2,369,1930GA 7.7% 162.9 / 2.5 52P16P (CCC→CCT) aroA3‑phosphoshikimate 1‑carboxyvinyltransferase (5‑enolpyruvoylshikimate‑3‑phosphate synthase)
Reads supporting (aligned to +/- strand):  ref base G (28/19);  new base A (2/2);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTGCGGTCGTCATCAGA  >  BS168_reference_genome/2369060‑2369325
                                                                                                                                     |                                                                                                                                     
tgtgAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGaa                                                                                                                                   >  7:187331/1‑138 (MQ=255)
     acacCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTa                                                                                                                             <  8:97126/139‑1 (MQ=255)
        cccATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCCGCTCCCGGCAGAAAGTTTTTCACAGTTGTTGTGCCCTTCGCTAGCGCGCCAAACATGACGGAACGGTGAGAAATGGATTTATCACCAGGAATATGTAttt                                                                                                                          >  3:308993/1‑139 (MQ=255)
           aTTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTtctc                                                                                                                       >  1:79516/1‑139 (MQ=255)
            tttttCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTtctc                                                                                                                       >  8:192357/1‑138 (MQ=255)
                 cTAAAGGAATCGATCGTTCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTTCCTGCCGCTAGCGCGCCAAACATAACTGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTTTTTCTCCATGt                                                                                                                  <  4:89724/138‑1 (MQ=255)
                 cTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGt                                                                                                                  <  3:36921/138‑1 (MQ=255)
                 cTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGt                                                                                                                  <  3:133252/138‑1 (MQ=255)
                    aaGAAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGATAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTa                                                                                                                 <  6:252423/136‑1 (MQ=255)
                     aGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGt                                                                                                                  <  5:39050/134‑1 (MQ=255)
                     aGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGt                                                                                                                  >  6:39050/1‑134 (MQ=255)
                        aaTCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTg                                                                                                          <  7:225583/139‑1 (MQ=255)
                          tcgatcgTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCa                                                                                                        >  8:2070/1‑139 (MQ=255)
                                      aGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGtt                                                                                            >  2:163371/1‑139 (MQ=255)
                                           aTCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCAtt                                                                                       >  2:308164/1‑139 (MQ=255)
                                            tCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAat                                                                                                                                  <  6:334068/95‑1 (MQ=255)
                                            tCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAat                                                                                                                                  >  5:334068/1‑95 (MQ=255)
                                            tCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTg                                                                                                          >  1:393502/1‑119 (MQ=255)
                                            tCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTg                                                                                                          <  2:393502/119‑1 (MQ=255)
                                            tCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCAtt‑t                                                                                     >  3:154013/1‑139 (MQ=255)
                                                cTCCCGGCAGAAAGTTTTTCACAGTTGTTGTGCCCTTCGCTAGCGCGCCAAACATGACGGAACGGTGAGAAATGGATTTATCACCAGGAATATGTATTTCTCCGTTTAAGGCCTGCACCTTTTCTCTTTTCAtt‑atttt                                                                                 <  4:308993/139‑2 (MQ=255)
                                                  cccGGCAGAAAGTTTTTCACAGTTGTTGTGCCCTTCGCTAGCGCGCCAAACATGACGGAACGGTGAGAAATGGATTTATCACCAGGAATATGTATTTCTCCGTTTAAGGCCTGCACCTTTTCTCTTTTCATTATTTTcc                                                                                >  8:238008/1‑139 (MQ=255)
                                                  cccGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGtt                                                                                            <  2:416565/127‑1 (MQ=255)
                                                  cccGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGtt                                                                                            >  1:416565/1‑127 (MQ=255)
                                                                        ttgttgtGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAat                                                                                                                                  >  5:151441/1‑67 (MQ=255)
                                                                        ttgttgtGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAat                                                                                                                                  <  6:151441/67‑1 (MQ=255)
                                                                                  tgccGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTcaga                                               >  4:375468/1‑136 (MQ=255)
                                                                                     cGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCg                                            <  2:79516/139‑1 (MQ=255)
                                                                                     cGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCg                                            <  2:50983/139‑1 (MQ=255)
                                                                                     cGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCAGAAAAAGTTTCATATTCCGCCCg                                            >  2:128803/1‑139 (MQ=255)
                                                                                            gcgcCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCTGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCa                                     <  7:2070/139‑1 (MQ=255)
                                                                                                 aaaCATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCa                                <  1:308164/139‑1 (MQ=255)
                                                                                                        aCAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAACTTTCATATTCCGCCAGGGCTTCAATGCTTTGTTCt                         >  2:130344/1‑139 (MQ=255)
                                                                                                            agCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGccc                     >  6:113334/1‑139 (MQ=255)
                                                                                                            agCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGccc                     >  6:184939/1‑139 (MQ=255)
                                                                                                            agCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGccc                     >  5:139640/1‑139 (MQ=255)
                                                                                                            agCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGccc                     >  3:385539/1‑139 (MQ=255)
                                                                                                            agCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGccc                     >  7:382811/1‑139 (MQ=255)
                                                                                                            agCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCAGT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATACCGCCCGGGCATCAATGCATAGTTCTGgcc                     >  8:116151/1‑139 (MQ=255)
                                                                                                            agCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCACGTATCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTACATATTCCGCCCGGGCTTCAATGCATTGTTCTGcc                      >  1:364787/1‑138 (MQ=255)
                                                                                                              cGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCtt                   >  7:158598/1‑139 (MQ=255)
                                                                                                              cGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTAATATTCCGCACGGGCTTCAATGCATTGTTCTGCCCtt                   >  5:235318/1‑139 (MQ=255)
                                                                                                                 tGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTg                <  5:184939/139‑1 (MQ=255)
                                                                                                                 tGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTg                <  5:113334/139‑1 (MQ=255)
                                                                                                                 tGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTg                >  4:249160/1‑139 (MQ=255)
                                                                                                                 tGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTg                <  8:187331/139‑1 (MQ=255)
                                                                                                                 tGAGAAATGGATTAATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTATCATT‑TTTTCCAACTCAATCAGCATAAAAAGTTTAATATTCCTCCAGGGCTTCAATGCATTGTTCTGCCCtattt                >  4:216112/1‑138 (MQ=255)
                                                                                                                     aaaTGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTGCGgt            <  1:163371/139‑1 (MQ=255)
                                                                                                                      aaTGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTGCGgt            <  6:139640/138‑1 (MQ=255)
                                                                                                                           aTTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTGCGGTCGtca       >  2:251934/1‑138 (MQ=255)
                                                                                                                              tATCACCAGGAATATGTATTTCTCCGTTTAAGGCCTGCACCTTTTCTCTTTTCATTATTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCGTTTGCGGTCGtcatc     <  7:238008/138‑1 (MQ=255)
                                                                                                                               aTCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTGCGGTCGTCATCAGa  >  8:371848/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TGTGAACACCCATTTTTCTAAAGCAATCGATCGTGCTCAGACAATCTGCTCCCGGCAGAAAGTTTTTAACTGTTGTTGTGCCTGCCGCTAGCGCGCCAAACATAACAGAGCGGTGAGAAATGGATTTATCACCGGGAATATGTATTTCTCCATGTAAGGTCTGCACCTTATCTCGTTTCATT‑TTTTCCACCTCAATCAGCATAAAAAGTTTCATATTCCGCCCGGGCTTCAATGCATTGTTCTGCCCTTTTGCGGTCGTCATCAGA  >  BS168_reference_genome/2369060‑2369325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑