Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 2,378,369 A→G 6.3% F244F (TTT→TTC)  aroB ← 3‑dehydroquinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome2,378,3690AG 6.3% 296.5 / 8.0 96F244F (TTT→TTC) aroB3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base A (51/38);  new base G (3/3);  total (54/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

ACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGC  >  BS168_reference_genome/2378231‑2378504
                                                                                                                                          |                                                                                                                                       
aCGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCa                                                                                                                                         <  6:371997/139‑1 (MQ=255)
          tCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaag                                                                                                                                >  1:140233/1‑138 (MQ=255)
             aTTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagtaag                                                                                                                            <  4:143971/139‑1 (MQ=255)
                            gTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCtctt                                                                                                             <  1:366585/139‑1 (MQ=255)
                                tttCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCa                                                                                                                                         >  4:270559/1‑107 (MQ=255)
                                tttCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCa                                                                                                                                         <  3:270559/107‑1 (MQ=255)
                                tttCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCttt                                                                                                         >  6:213800/1‑139 (MQ=255)
                                     cTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCAt                                                                                                    >  1:236635/1‑139 (MQ=255)
                                         tatataAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATc                                                                                                                    >  7:1044/1‑119 (MQ=255)
                                         tatataAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATc                                                                                                                    <  8:1044/119‑1 (MQ=255)
                                         tatataAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCtctt                                                                                                             <  6:362453/126‑1 (MQ=255)
                                         tatataAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCtctt                                                                                                             >  5:362453/1‑126 (MQ=255)
                                             taAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCctgctg                                                                                             <  2:105013/138‑1 (MQ=255)
                                                aGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCCTATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAAc                                                                                          >  6:420801/1‑138 (MQ=255)
                                                aGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCtctt                                                                                                             >  7:267221/1‑119 (MQ=255)
                                                aGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCtctt                                                                                                             <  8:267221/119‑1 (MQ=255)
                                                aGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTGCTTTTTCATCCTGCTGAAc                                                                                          >  4:117955/1‑138 (MQ=255)
                                                aGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCACCTCTTCTTTTTCATGCTGCTGAACg                                                                                         >  6:234868/1‑139 (MQ=255)
                                                aGCAAACTGCATTCCGAGGGCTACGGCATCACCCTGAGAGATCTGACCATATCCAAATTCCGCATCAAAGGCATGCCCTAGCTTATGCACAAAGTTTAAGAAATCTCTAATACCCTCTTCTTTTTCATCATGCTGAAc                                                                                          >  6:204152/1‑138 (MQ=255)
                                                    aaCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAg                                                                                     >  7:26858/1‑139 (MQ=255)
                                                     aCTGCATTGCGAGGGCTACGGCATCAGCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                                    <  4:164188/139‑1 (MQ=255)
                                                     aCTGCATTCCTAGGTCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCTCCTCAACGGCATCGCCGAGCGTATGACCAAAGTTTAAGTAAGCTCTTATCCCATCATCTTTTTCATCATGCTGAACGAAAGa                                                                                    >  6:1106/1‑139 (MQ=255)
                                                     aCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                                    >  6:138264/1‑139 (MQ=255)
                                                     aCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                                    >  1:130712/1‑139 (MQ=255)
                                                     aCTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGATTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGa                                                                                    >  7:350702/1‑139 (MQ=255)
                                                         cATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagt                                                                                                                               <  7:88804/92‑1 (MQ=255)
                                                         cATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagt                                                                                                                               >  8:88804/1‑92 (MQ=255)
                                                           tcccGAGGGCTACGGCATCACCGTGAGTGATCTGTCCGTATCCATATTCAGCCTCAACGGCATGGCCGAGCGTATGTCCGAAGTTTAAGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCttt                                                                              >  6:226527/3‑139 (MQ=255)
                                                           tcccGAGGGCTACGGCATCACCGTGAGTGATCTGTCCGTATCCATATTCAGCCTCAACGGCATGGCCGAGCGTATGTCCGAAGTTTAAGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCttt                                                                              >  6:232133/3‑139 (MQ=255)
                                                                 gggCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTg                                                                         >  2:50444/1‑138 (MQ=255)
                                                                   gCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGaaa                                                                      <  8:26858/139‑1 (MQ=255)
                                                                   gCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGaaa                                                                      >  6:44203/1‑139 (MQ=255)
                                                                        ggCATCACCGTGAGTGATCTGCCCCTATCCATATTCCGCCTCAACGGCATGGCCGCGCGTATGCCCAAAGTTTAAGTACGCTCTTCTCCCCTCTTCGTTTTCAGCCGGCGGCCCGACAGCCGCGTTCGTTGaaagggct                                                                 >  4:233301/1‑134 (MQ=255)
                                                                            tCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTa                                                              >  7:110942/1‑138 (MQ=255)
                                                                             cACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGCGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAAGGACAGACGCTTTAATTGAAATGCCTTTaa                                                             >  5:59570/1‑138 (MQ=255)
                                                                             cACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTaa                                                             >  7:31532/1‑138 (MQ=255)
                                                                               ccGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTaaaaa                                                          <  6:353465/139‑1 (MQ=255)
                                                                               ccGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTaaaaa                                                          <  1:50444/139‑1 (MQ=255)
                                                                                  tgagtgaTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTATTTGAAATGCCTTTAAAAATCa                                                       >  8:359987/1‑139 (MQ=255)
                                                                                    agtgaTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagta                                                                                                                              <  1:199851/66‑1 (MQ=255)
                                                                                    agtgaTCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagta                                                                                                                              >  2:199851/1‑66 (MQ=255)
                                                                                         tCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCAtt                                                >  2:117952/1‑139 (MQ=255)
                                                                                         tCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCAt                                                 >  3:353607/1‑138 (MQ=255)
                                                                                         tCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCAt                                                 >  1:330874/1‑138 (MQ=255)
                                                                                          cTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCAtt                                                >  6:214491/1‑138 (MQ=255)
                                                                                          cTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTAATACTGCTGAACTAAAGACGCTTTAATTTAAATGCATTTAAAAAAAATGTCAtt                                                >  1:365578/1‑138 (MQ=255)
                                                                                             cccATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAg                                             <  5:213800/138‑1 (MQ=255)
                                                                                             cccATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAg                                             <  8:31532/138‑1 (MQ=255)
                                                                                              ccatatccatatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGATTAAGTAAGCTCTTATCCCCTCTTCTTTTTCTTCCTGCTGTTCGACAGACTCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCt                                           <  5:204152/139‑1 (MQ=255)
                                                                                                       tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagt                                                                                                                               <  5:74637/46‑1 (MQ=255)
                                                                                                       tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagt                                                                                                                               >  6:74637/1‑46 (MQ=255)
                                                                                                       tatTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGCACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAg                                  >  5:75990/1‑139 (MQ=255)
                                                                                                       tatTCAGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAg                                  <  3:262549/139‑1 (MQ=255)
                                                                                                         ttCCGCCTCAACGGCATGGCCGAGCGTATGCCCTAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTg                                <  4:98913/139‑1 (MQ=255)
                                                                                                         ttCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTg                                <  1:214700/139‑1 (MQ=255)
                                                                                                            cGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTTATTGAAATGCCTTTAAAAATCATGTCATTCAGGTGAGCATTAGTGata                             >  4:48968/1‑139 (MQ=255)
                                                                                                            cGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGata                             >  1:380140/1‑139 (MQ=255)
                                                                                                            cGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGat                              <  8:110942/138‑1 (MQ=255)
                                                                                                            cGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATACTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGata                             >  5:75706/1‑139 (MQ=255)
                                                                                                             gCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTg                                <  2:311786/135‑1 (MQ=255)
                                                                                                             gCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTg                                >  1:311786/1‑135 (MQ=255)
                                                                                                              ccTCAACGTCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  5:356278/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGTCCGAAGTTTAAGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCTTTTATTGAAATCCCTTTAAAAATCATGTCATTCAGCTGATCACTGGTg                                >  2:171135/1‑134 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGTCCGAAGTTTAAGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCTTTTATTGAAATCCCTTTAAAAATCATGTCATTCAGCTGATCACTGGTg                                <  1:171135/134‑1 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagta                                                                                                                              >  4:81602/1‑40 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTtaagta                                                                                                                              <  3:81602/40‑1 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATTTCATTAATCTGATCATTAGTTatat                            >  8:347024/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAg                                             <  8:183547/121‑1 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAg                                             >  7:183547/1‑121 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            <  8:258082/138‑1 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  7:258082/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  7:21614/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  7:15415/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  8:98315/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  1:328355/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  2:109750/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  2:258107/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  5:18666/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            <  2:328355/138‑1 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGatat                            >  2:335775/1‑138 (MQ=255)
                                                                                                              ccTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATATCATTCAGCTGATCATTAGTGatat                            >  8:204443/1‑138 (MQ=255)
                                                                                                                 cAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATACTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACg                        <  6:75706/139‑1 (MQ=255)
                                                                                                                  aaCGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACg                        <  6:400402/138‑1 (MQ=255)
                                                                                                                  aaCGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACg                        <  5:71402/138‑1 (MQ=255)
                                                                                                                   aCGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCa                      <  7:189271/139‑1 (MQ=255)
                                                                                                                     ggCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCagt                    <  4:353607/139‑1 (MQ=255)
                                                                                                                     ggCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCagt                    <  2:330874/139‑1 (MQ=255)
                                                                                                                         tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCagtgagt                <  1:258107/139‑1 (MQ=255)
                                                                                                                         tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCagtgagt                >  2:237695/1‑139 (MQ=255)
                                                                                                                         tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCagtgagt                <  6:18666/139‑1 (MQ=255)
                                                                                                                         tGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCGTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACTCagtgagt                <  5:1106/139‑1 (MQ=255)
                                                                                                                              gAGCGTATGTCCGAAGTTTAAGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCTTTTATTGAAATCCCTTTAAAAATCATGTCATTCAGCTGATCACTGGTGATATCACGCAGTGAGCGGata           <  5:226527/139‑1 (MQ=255)
                                                                                                                              gAGCGTATGTCCGAAGTTTAAGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCTTTTATTGAAATCCCTTTAAAAATCATGTCATTCAGCTGATCACTGGTGATATCACGCAGTGAGCGGata           <  5:232133/139‑1 (MQ=255)
                                                                                                                               aGCGTATGACCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTATTTCATCCTGCTGAACGACAGACGCTTTAATTGAAAAGCCTTTAAAAATCATGTCATTCAGAAGAAAATAAGTCATATCACGCAGACAGTGGatat          >  6:135337/1‑139 (MQ=255)
                                                                                                                                     tGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTcagca    <  8:21614/139‑1 (MQ=255)
                                                                                                                                     tGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTcagca    <  8:15415/139‑1 (MQ=255)
                                                                                                                                       cccAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTcagcag   <  2:31597/138‑1 (MQ=255)
                                                                                                                                       cccAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCagcagc  <  8:350702/139‑1 (MQ=255)
                                                                                                                                       cccAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCagcagc  <  6:75990/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
ACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACGGCATGGCCGAGCGTATGCCCAAAGTTTAAGTAAGCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGC  >  BS168_reference_genome/2378231‑2378504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑