Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | BS168_reference_genome | 2,990,552 | G→A | 6.1% | A194V (GCG→GTG) | maeB ← | NADP‑dependent malic enzyme (conversion of malate into pyruvate, anabolic) |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | BS168_reference_genome | 2,990,552 | 0 | G | A | 6.1% | 218.9 / 2.8 | 65 | A194V (GCG→GTG) | maeB | NADP‑dependent malic enzyme (conversion of malate into pyruvate, anabolic) |
Reads supporting (aligned to +/- strand): ref base G (33/28); new base A (2/2); total (35/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-01 |
TGAACTTCGCCACTTCATTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGATCATCATGGAAAACCGGGATGTTTGTTTCTT > BS168_reference_genome/2990418‑2990687 | tGAACTTCGCCACTTCATTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGcccc > 8:253545/1‑139 (MQ=255) tGAACTTCGCCACTTCATTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCAcc > 6:338258/1‑139 (MQ=255) tCGCCACTTCATTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGcc > 1:26999/1‑139 (MQ=255) cGCCACTTCATTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCg > 1:308321/1‑139 (MQ=255) cGCCACTTCATTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCg > 1:313974/1‑139 (MQ=255) ccACTTCATTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATCTCGCCAACCCCATAAAGATTAACAAGCTTGATAATCGCAATGACAGCCGCACCCGCGCCgat > 5:315659/1‑137 (MQ=255) aTTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGAc < 8:365104/138‑1 (MQ=255) aTTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGAc < 5:338258/138‑1 (MQ=255) tCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATgg < 3:406799/139‑1 (MQ=255) ttCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGAtt > 7:355280/1‑139 (MQ=255) gggCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTc < 2:26999/139‑1 (MQ=255) gcCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGa < 8:15725/139‑1 (MQ=255) gcCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGa < 8:137538/139‑1 (MQ=255) gcCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCGTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGa < 6:417684/139‑1 (MQ=255) tttGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCACACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGa > 6:82547/1‑120 (MQ=255) tttGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCACACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGa < 5:82547/120‑1 (MQ=255) ttGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGGTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACaa > 5:20797/1‑139 (MQ=255) gAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGAc < 7:253545/139‑1 (MQ=255) tCGCACATCACTATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAAttg < 5:202207/94‑3 (MQ=255) tCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATgg > 6:202207/1‑94 (MQ=255) cacaTCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTa > 1:384368/1‑139 (MQ=255) cacaTCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCCTTCACAAGACCTGCTa > 3:399357/1‑139 (MQ=255) cacaATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACgggt > 4:75049/1‑137 (MQ=255) caATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc < 5:172138/139‑1 (MQ=255) caATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc < 3:364/139‑1 (MQ=255) caATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGAAATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc < 5:234370/139‑1 (MQ=255) caATGTCGCGAACGCCATAATGAAGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc < 3:41014/139‑1 (MQ=255) aaTGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc > 5:162820/1‑138 (MQ=255) tGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAAt > 5:5805/1‑139 (MQ=255) tGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAAt > 5:368294/1‑139 (MQ=255) gcgAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCa < 2:313974/139‑1 (MQ=255) gcgAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCa < 2:308321/139‑1 (MQ=255) gcgAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCa < 8:399305/139‑1 (MQ=255) cgAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGAc < 7:27649/81‑1 (MQ=255) cgAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGAc > 8:27649/1‑81 (MQ=255) cgAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc > 7:70959/1‑131 (MQ=255) cgAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc < 8:70959/131‑1 (MQ=255) cgAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAg > 8:403080/1‑139 (MQ=255) cgAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAg > 7:361644/1‑139 (MQ=255) aaCGCCATAATGAGGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGAGTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTa < 4:38135/139‑1 (MQ=255) atgaAGTAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGatca < 1:139765/139‑1 (MQ=255) tgaAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGAc > 4:9615/1‑50 (MQ=255) tgaAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGAc < 3:9615/50‑1 (MQ=255) tgaAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGa < 4:13311/80‑1 (MQ=255) tgaAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGa > 3:13311/1‑80 (MQ=255) tgaAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc > 8:269638/1‑116 (MQ=255) tgaAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc < 7:269638/116‑1 (MQ=255) tgaAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGatcat > 1:202835/1‑139 (MQ=255) tgaAGAAGCTTGATAATCGCAATGCCAGCCACACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc > 8:274875/1‑116 (MQ=255) tgaAGAAGCTTGATAATCGCAATGCCAGCCACACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc < 7:274875/116‑1 (MQ=255) aagaagCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGtt > 1:362582/1‑42 (MQ=255) aagaagCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGtt < 2:362582/42‑1 (MQ=255) aagaagCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc < 4:423804/114‑1 (MQ=255) aagaagCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTAc > 3:423804/1‑114 (MQ=255) aagaagCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGAtcatca > 3:152842/1‑139 (MQ=255) aagaagCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGAtcatca > 3:280390/1‑139 (MQ=255) aagaagCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGAtcatca > 1:259717/1‑139 (MQ=255) gCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGa > 3:299209/1‑73 (MQ=255) gCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGa < 4:299209/73‑1 (MQ=255) cTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGATCATCATGGaa > 2:61781/1‑138 (MQ=255) tCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGATCATCATGGAAAACCGGGAt < 6:5805/139‑1 (MQ=255) tCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGATCATCATGGAAAACCGGGAt < 6:368294/139‑1 (MQ=255) gCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCCCCTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGATCAACATGGAAAACCGGGAtgt > 1:142972/1‑139 (MQ=255) cAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGATCATCATGGAAAACCGGGAtgtt > 7:293985/1‑139 (MQ=255) gCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGGCATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTCCCGTGCTGCTCATCATGGAATACCGGGATGTTTGTTTCtt > 1:78717/1‑139 (MQ=255) | TGAACTTCGCCACTTCATTTTTGACATCGTTCATGCCGTTCGGGCGCCCTTCATAAATGGCCCCTTTGGAATCGCACATCACAATGTCGCGAACGCCATAATGATGAAGAAGCTTGATAATCGCAATGCCAGCCGCACCCGCGCCGTTTGCGACAACTTTAATGGATGACATGGATTTTCCAGACAGTTTCAGCGCGTTCACAAGACCTGCTACGGTTACAATGGCAGTACCGTGCTGATCATCATGGAAAACCGGGATGTTTGTTTCTT > BS168_reference_genome/2990418‑2990687 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |