| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | BS168_reference_genome | 3,935,825 | Δ1 bp | coding (1190/1191 nt) | rlmI ← | large subunit ribosomal RNA m5C1962 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 3,935,823 | 0 | T | . | 96.8% | 232.4 / 3.8 | 63 | intergenic (+35/+1) | ywbE/rlmI | conserved protein of unknown function/large subunit ribosomal RNA m5C1962 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); major base . (32/28); minor base A (2/0); total (35/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
TGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCATTTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGC > BS168_reference_genome/3935688‑3935949 | tGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCat > 5:149699/1‑136 (MQ=255) gATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCattattt > 1:149909/1‑132 (MQ=255) atTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTAtttttt > 1:274964/1‑136 (MQ=255) atTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGATGCCCCTGAACAGGGCAGCTTTTTCA‑TTAttttt > 3:338315/1‑135 (MQ=255) tttGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGCCCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCt > 6:58372/1‑136 (MQ=255) ttGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCATTTTGA‑TTATTTTTTCt > 6:274563/1‑135 (MQ=255) gACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGCCCAGCAGCTCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGACCATGGCAGCGTTTTCA‑GTATTTTTTCTGcg > 3:230935/1‑135 (MQ=255) gACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCCGATCGTTTCTACAT‑AAAAAAAACCTGCCCCTGAACAGGGCAGCATTTTCA‑TTATTGTTTCTg > 2:142784/1‑134 (MQ=255) gACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTg > 3:202126/1‑134 (MQ=255) aaaaCAACCTTTCACCCGCATGCCAATAAAGGCCGTCTTGACGAGGGCCCCCGCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAg < 3:146276/136‑1 (MQ=255) aaTCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCCTCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAATCAGACCCGGAACAGGGCAGCTGTTTCA‑TTATTTTTTCTGCAGCAgg > 2:7981/1‑136 (MQ=255) ccGCATGGCATTAACGGCCGCCTTCACGAGGGCCGCAGCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTa < 1:3340/136‑1 (MQ=255) cGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCAGCAGAAGGACCACGTT‑CAGGtct > 7:395001/1‑134 (MQ=255) ggCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGcc > 4:82243/1‑136 (MQ=255) ttttcaaGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCg < 8:130347/131‑1 (MQ=255) tCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGcc > 3:375575/1‑135 (MQ=255) cTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGGAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCCCTTT‑CAGGTAGTTGCCTTCCGGAAATGCCg > 4:69775/1‑135 (MQ=255) cTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCg > 4:384196/1‑135 (MQ=255) cTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCg > 1:313684/1‑135 (MQ=255) cTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAACTGCCCCTGAAAAGGGAAGCTTTTT‑AATTATTTTTTGAGAAATATGCCCACTTT‑GAGAGAGTTGACATCCGGAAAAGCCg > 2:393397/1‑135 (MQ=255) aTGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAg > 8:4063/1‑93 (MQ=255) aTGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAg < 7:4063/93‑1 (MQ=255) ggCCGCACCCCCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGttt < 5:259164/135‑1 (MQ=255) cGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGCCCACTTT‑CCGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGa > 6:2433/1‑135 (MQ=255) cGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTATTTGCCTTCCGGAAATGCCTAAATAGTTTTGa > 6:16909/1‑135 (MQ=255) gCATCGGCCGCGACCAGCAGATCGTATCTACAT‑AAAAAAAAACTGCCCCTGAACAGGGCAGCTATTT‑AATTATTTTTTCTTCAGAAGGACAACTTT‑AAGTTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGaa > 2:177392/1‑135 (MQ=255) gcccGCGACCCCCAGTTGCTTTCTAAAA‑AAAAAACAGCTGCCCCTCAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAAtcctc < 1:393397/134‑1 (MQ=255) gCCGCGACCAGCAGATCTTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAAtcctcc < 4:375575/136‑1 (MQ=255) cccGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAAtcctcc < 1:296442/135‑1 (MQ=255) ccGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCg > 8:279715/1‑136 (MQ=255) gcgACCAGCAGATCGTTTCTACATAAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAg > 6:180480/1‑79 (MQ=255) gcgACCAGCAGATCGTTTCTACATAAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAg < 5:180480/79‑1 (MQ=255) gcgACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCGTTTTGATTT‑TTTTTTCTGCCGAAGGACCACTTT‑CAGGTAGTTGCCGTCCGGTAATGCCGAAATCGGTTTGAAATCCTCCGGc > 5:267555/1‑136 (MQ=255) gACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAAACGGGCAGCTTTTTGA‑TTATTTTTTTTTCATAATGACCAATTTACGGGTA‑TTTCCTTACCGAAATTCCCTAAACCTTTTTTAATCCCACGGCAg > 2:203959/1‑136 (MQ=255) aCCAGCTGAGAGTTTATACAT‑AAAAAAACGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CTGGTAGTTGCCTTCCGGAAATGCAGAAATCGTTTTGAAATCCTCCGGCAGc < 3:72080/136‑1 (MQ=255) cagcagATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGt < 7:22590/136‑1 (MQ=255) agcagATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTa < 7:3364/136‑1 (MQ=255) agcagATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTATGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGt > 1:352417/1‑135 (MQ=255) ttCGTTTATACAT‑AAAAAAACGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTc < 4:48239/135‑1 (MQ=255) cGTTTCTACAA‑AAAAAAACCCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCt < 4:19504/136‑1 (MQ=255) gTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CATGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCt < 8:114534/135‑1 (MQ=255) tCTAAAA‑AAAAAAAACCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGTCCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCaa < 5:274563/135‑1 (MQ=255) aaaaaaGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTa < 3:82243/136‑1 (MQ=255) aaaaaaGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTa < 3:384196/136‑1 (MQ=255) aaaaaGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAg < 1:358315/136‑1 (MQ=255) aaaGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCg > 1:363995/1‑136 (MQ=255) ccccTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCAtt < 1:320389/135‑1 (MQ=255) cTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGtt > 1:7195/1‑136 (MQ=255) cTGAAAAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGCAGGACCACTTT‑CAGGTAGTTGCCGTCCCGAAAAGCCGAAATAGGTTAGAAATACTCCGGCAGCGTAAATTCCACAATGAGCGAGTTGCGTTCCATTGtt > 6:130318/1‑136 (MQ=255) cAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGAGCGTGTAGCGGTCATGTGGTTCttt > 6:126431/1‑136 (MQ=255) gggCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTaa < 8:185445/136‑1 (MQ=255) ggCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTaaa < 4:274175/136‑1 (MQ=255) cAGCTTTTTCA‑TTATTTTTTCTGCAGCAGGACCACGTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCCGCGTAAATTCCTCAATGATCGTGTAGCGTGCATTTGTTTCTTTAAAgg > 7:11518/1‑136 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAGATCCTCCGGCAGCGTAAATTCCTCAATGAGCGTGTAGCGTTCATTTGTTTCTTTAAAggc > 2:33907/1‑136 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAAtcctcc < 6:223880/83‑1 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAAtcctcc > 5:223880/1‑83 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGt < 2:368109/91‑1 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGt > 1:368109/1‑91 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCgt > 1:374764/1‑109 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCgt < 2:374764/109‑1 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggc < 7:279715/136‑1 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggc > 5:102307/1‑136 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggc > 1:27798/1‑136 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggc < 2:313684/136‑1 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggc < 1:142784/136‑1 (MQ=255) | TGAAGGATATTTTGACGAAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCATTTATTTTTTCTGCAGAAGGACCACTTT‑CAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGC > BS168_reference_genome/3935688‑3935949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |