Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	165,751	Δ1 bp	intergenic (+44/‑3)	rrnI‑5S → / → trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,751	0	C	.	100.0%	275.7 / NA	80	intergenic (+44/‑3)	rrnI‑5S/trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (34/46);  total (34/46)

CCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAG  >  BS168_reference_genome/165625‑165868                                                                                                                               |                                                                                                                       ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGTTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                             <  7:395154/136‑1 (MQ=255) ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                               >  5:332280/1‑134 (MQ=255) ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                               >  1:269350/1‑134 (MQ=255) ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                             <  4:327790/136‑1 (MQ=255) ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                             <  8:285714/136‑1 (MQ=255) ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCAAGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                             <  8:121724/136‑1 (MQ=255) ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCGGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                             <  1:267959/136‑1 (MQ=255) ccATACCGAAACCGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                             <  5:95530/136‑1 (MQ=21)  cATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTTATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                            <  3:264416/136‑1 (MQ=255)  cATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                            <  1:160265/136‑1 (MQ=255)  cATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                            <  4:86906/136‑1 (MQ=255)      ccGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                          >  7:264089/1‑134 (MQ=255)       cGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAg                                                                                                       <  4:393489/136‑1 (MQ=255)       cGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAg                                                                                                       <  4:363699/136‑1 (MQ=255)        gAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGt                                                                                                      >  7:146139/1‑136 (MQ=255)         aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTg                                                                                                     <  6:63845/136‑1 (MQ=255)         aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTg                                                                                                     <  3:57092/136‑1 (MQ=255)         aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTg                                                                                                     <  7:305684/136‑1 (MQ=255)         aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTg                                                                                                     <  2:312471/136‑1 (MQ=255)         aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTg                                                                                                     <  1:66001/136‑1 (MQ=255)           cacGGAAGTTAAGCTCTTCAGCGCCGATGGTAGGCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGt                                                                                                   <  5:133809/136‑1 (MQ=255)            acGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                    >  4:45206/1‑134 (MQ=255)            acGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTg                                                                                                     >  6:378537/1‑133 (MQ=255)            acGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTg                                                                                                     <  5:378537/133‑1 (MQ=255)            acGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                  <  5:50013/136‑1 (MQ=255)            acGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCAGCAGTAGCTCAGTgg                                                                                                    >  6:154459/1‑134 (MQ=255)            acGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTACGCAGTAGCTCAGTGGTa                                                                                                  >  1:392784/1‑136 (MQ=255)            acGGAAGTTAAGATCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                    >  6:388618/1‑134 (MQ=255)                acgTTAAGCTCTTACGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                              <  5:272930/134‑1 (MQ=25)                aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                              <  3:345418/136‑1 (MQ=255)                 aGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                              >  6:291742/1‑135 (MQ=255)                             cAGCGCCGATGGTAGTCGCGGGTTTCCCCCTGAGAGAGTACTACGCCGCCAAGCAGAATTATTAATTATACCAATTTTGTTTATGAAACAATAAAT‑T‑ATTCAGCACTATCTCAGTGGTAGAGATATCTGCTGTTa                                                                                  >  2:154419/1‑135 (MQ=16)                              aGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAc                                                                                <  1:265901/136‑1 (MQ=255)                              aGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAc                                                                                <  7:223490/136‑1 (MQ=255)                               gcgcCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAcc                                                                               <  5:388618/136‑1 (MQ=255)                                cgcCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAcc                                                                               >  3:418976/1‑135 (MQ=255)                                        gTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTACGCAGTAGCTCATTGGTATAGCTATCGGCTGTTAACCGATCGGTCg                                                                      >  1:32840/1‑136 (MQ=38)                                                gggTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCg                                                              <  3:88113/136‑1 (MQ=255)                                                gggTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCg                                                              <  5:291742/136‑1 (MQ=255)                                                          cTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCt                                                    <  4:361359/136‑1 (MQ=255)                                                             tgAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTGTAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTg                                                   <  1:21837/134‑1 (MQ=255)                                                                gagTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAg                                              >  5:366187/1‑136 (MQ=255)                                                                  gTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATTT‑‑TTCCGCAGTAGCTCTGTGGTAGAGCTATCCGCTGTTAACCGATCGGTCGTAGGTTCGACTCCTACCTGCGgcg‑c                                            >  1:390676/1‑135 (MQ=21)                                                                    aGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                              >  1:316604/1‑84 (MQ=38)                                                                    aGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                              <  2:316604/84‑1 (MQ=38)                                                                    aGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATc                                                                                          <  8:330651/88‑1 (MQ=38)                                                                    aGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATc                                                                                          >  7:330651/1‑88 (MQ=38)                                                                    aGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTATCCGAGCGGTCTTAGTTTCGAATCATACCTGCTGAG‑Caat                                         >  5:46761/1‑134 (MQ=21)                                                                    aGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccat                                         >  1:59037/1‑133 (MQ=255)                                                                      gACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑cc                                           >  5:377512/1‑131 (MQ=255)                                                                      gACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑cc                                           <  6:377512/132‑2 (MQ=255)                                                                       aCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGc                                       <  5:154459/136‑1 (MQ=18)                                                                         gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                              >  4:229775/1‑79 (MQ=38)                                                                         gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                              <  3:229775/79‑1 (MQ=38)                                                                         gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCtt                                     >  8:201472/1‑136 (MQ=18)                                                                         gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCtt                                     >  4:101333/1‑136 (MQ=18)                                                                         gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCtt                                     <  2:269350/136‑1 (MQ=18)                                                                         gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCtt                                     <  4:418976/136‑1 (MQ=18)                                                                            gccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTcc                                   >  6:224771/1‑135 (MQ=18)                                                                              cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                  >  4:181944/1‑134 (MQ=18)                                                                              cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                  >  3:172730/1‑134 (MQ=18)                                                                              cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                  >  5:85319/1‑134 (MQ=18)                                                                                  cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCTGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGGCGTATGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTc                            <  1:161116/136‑1 (MQ=18)                                                                                  cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTc                            <  6:366187/136‑1 (MQ=18)                                                                                        tattaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                      >  7:190815/1‑136 (MQ=18)                                                                                          ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTa                    <  8:264089/136‑1 (MQ=18)                                                                                                  aCCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTc                            <  1:83871/120‑1 (MQ=17)                                                                                                  aCCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTc                            >  2:83871/1‑120 (MQ=17)                                                                                                  aCCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt            >  7:318584/1‑136 (MQ=18)                                                                                                   ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt            <  7:308611/135‑1 (MQ=18)                                                                                                   ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt            <  3:101333/135‑1 (MQ=18)                                                                                                   ccATGTTTGTTTAGGATGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt            <  2:392784/135‑1 (MQ=18)                                                                                                    cATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc           >  8:369382/1‑135 (MQ=18)                                                                                                     aTGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCGCCGTGGTCGAGCTATCGGCTGTTAACCGATCGGTCGTAGGGTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCGCAGCAGGTAGAGCACTTCCa         <  3:118518/136‑1 (MQ=18)                                                                                                      tgtttgttTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCt                                                    <  3:127423/92‑1 (MQ=17)                                                                                                      tgtttgttTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCt                                                    >  4:127423/1‑92 (MQ=17)                                                                                                       gtttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATg       >  2:309975/1‑136 (MQ=18)                                                                                                        tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATg       <  7:369382/135‑1 (MQ=18)                                                                                                            tttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAg  >  6:48972/1‑136 (MQ=18)                                                                                                            attATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAg  <  6:407093/135‑1 (MQ=18)                                                                                                                               |                                                                                                                       CCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAG  >  BS168_reference_genome/165625‑165868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑