Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	165,826	+C	intergenic (+1/‑4)	trnI‑Asn → / → trnI‑Thr	tRNA‑Asn/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,825	1	.	C	97.7%	290.8 / ‑0.4	88	noncoding (72/72 nt)	trnI‑Asn	tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (48/38);  total (49/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑C‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTG  >  BS168_reference_genome/165688‑165952                                                                                                                                           |                                                                                                                                 gagTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAg                                                                                                                                   >  5:366187/1‑136 (MQ=255)   gTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATTT‑‑TTCCGCAGTAGCTCTGTGGTAGAGCTATCCGCTGTTAACCGATCGGTCGTAGGTTCGACTCCTACCTGCGgcg‑c                                                                                                                                 >  1:390676/1‑135 (MQ=21)     aGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTATCCGAGCGGTCTTAGTTTCGAATCATACCTGCTGAG‑C‑aat                                                                                                                             >  5:46761/1‑134 (MQ=21)     aGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccat                                                                                                                              >  1:59037/1‑133 (MQ=255)       gACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑cc                                                                                                                                >  5:377512/1‑131 (MQ=255)       gACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑cc                                                                                                                                <  6:377512/132‑2 (MQ=255)        aCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGc                                                                                                                           <  5:154459/136‑1 (MQ=18)          gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCtt                                                                                                                         >  4:101333/1‑136 (MQ=18)          gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCtt                                                                                                                         <  4:418976/136‑1 (MQ=18)          gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCtt                                                                                                                         >  8:201472/1‑136 (MQ=18)          gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCtt                                                                                                                         <  2:269350/136‑1 (MQ=18)             gccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTcc                                                                                                                       >  6:224771/1‑135 (MQ=18)               cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCa                                                                                                                      >  5:85319/1‑134 (MQ=18)               cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCa                                                                                                                      >  4:181944/1‑134 (MQ=18)               cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCa                                                                                                                      >  3:172730/1‑134 (MQ=18)                   cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCTGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGGCGTATGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTc                                                                                                                <  1:161116/136‑1 (MQ=18)                   cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTc                                                                                                                <  6:366187/136‑1 (MQ=18)                         tattaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAgg                                                                                                          >  7:190815/1‑136 (MQ=18)                           ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTa                                                                                                        <  8:264089/136‑1 (MQ=18)                                   aCCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTc                                                                                                                >  2:83871/1‑120 (MQ=17)                                   aCCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTc                                                                                                                <  1:83871/120‑1 (MQ=17)                                   aCCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                >  7:318584/1‑136 (MQ=18)                                    ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                <  3:101333/135‑1 (MQ=18)                                    ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                <  7:308611/135‑1 (MQ=18)                                    ccATGTTTGTTTAGGATGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                <  2:392784/135‑1 (MQ=18)                                     cATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                                                                                               >  8:369382/1‑135 (MQ=18)                                      aTGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCGCCGTGGTCGAGCTATCGGCTGTTAACCGATCGGTCGTAGGGTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCGCAGCAGGTAGAGCACTTCCa                                                                                             <  3:118518/136‑1 (MQ=18)                                        gtttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATg                                                                                           >  2:309975/1‑136 (MQ=18)                                         tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATg                                                                                           <  7:369382/135‑1 (MQ=18)                                             tttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAg                                                                                      >  6:48972/1‑136 (MQ=18)                                             attATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAg                                                                                      <  6:407093/135‑1 (MQ=18)                                                              attaTTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGt                                                                       >  3:403823/4‑136 (MQ=255)                                                               ttaTTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGGCAGCGGtt                                                                      <  6:332280/134‑1 (MQ=255)                                                                     cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTc                                                                                                                <  2:261179/88‑1 (MQ=255)                                                                     cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTc                                                                                                                >  1:261179/1‑88 (MQ=255)                                                                     cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTa                                                                                                        >  7:77730/1‑96 (MQ=255)                                                                     cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTa                                                                                                        <  8:77730/96‑1 (MQ=255)                                                                     cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCAt                                                                                            <  6:341747/108‑1 (MQ=255)                                                                     cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCAt                                                                                            >  5:341747/1‑108 (MQ=255)                                                                     cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                >  3:50706/1‑136 (MQ=255)                                                                     cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                >  7:166392/1‑136 (MQ=255)                                                                        aGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTa                                                                                                        <  7:292772/93‑1 (MQ=255)                                                                        aGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTa                                                                                                        >  8:292772/1‑93 (MQ=255)                                                                        aGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGc                                                             >  1:205525/1‑136 (MQ=255)                                                                        aGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGc                                                             >  6:109481/1‑136 (MQ=255)                                                                        aGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGc                                                             >  2:307259/1‑136 (MQ=255)                                                                         gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGGCGTAGGGTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCt                                                            <  3:68980/136‑1 (MQ=255)                                                                                attGGTAGAGCTATCTGCTGTTAACCGATCGGTCGTAGGTTCGAATCATACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAg                                                      <  2:32840/133‑1 (MQ=37)                                                                                aGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGaa                                                       <  8:190815/134‑1 (MQ=255)                                                                                aGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGaa                                                       <  2:284537/134‑1 (MQ=255)                                                                                  tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCtt                                                   <  5:224771/136‑1 (MQ=255)                                                                                   ggTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTa                                                  <  8:318584/136‑1 (MQ=255)                                                                                    gTAGAGCTATCGGGTGTTAACCGATCGGTCGTAGGTTCGAATACTACCTGCTGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTTAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTa                                                  <  4:384415/135‑1 (MQ=37)                                                                                     tAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                >  7:178744/1‑136 (MQ=255)                                                                                       gagCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATg                                              >  5:312727/1‑136 (MQ=255)                                                                                       gagCTATCGGCTGTTAACCGATATTTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATg                                              >  7:320118/1‑136 (MQ=38)                                                                                          cTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCGATAGCTCAGCAGGTAGAGCACTTCAATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtat                                           >  5:259971/1‑136 (MQ=255)                                                                                             tCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtattat                                        >  3:194280/1‑136 (MQ=255)                                                                                             tCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtattat                                        >  2:285651/1‑136 (MQ=255)                                                                                              cGGCTGTTATCCGATCGGGCGGAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCATCCATAGCGCAGCATGTAGAGGAGTTCCATGGTAAGGAAGAGGTCCGCGGTTCGCGCCCGCTTGGAAGCTTAAATGAataatt                                       <  2:390676/136‑1 (MQ=25)                                                                                               ggCTGTTAACCTATCGGTCGTAGGTTCGATTCCTACATGCGGAGCC‑ATTGCTTCAATAGCTCACCAGGGAGAGCAATTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCGTGGAAGCTTAAATGTattatt                                       >  5:335817/1‑135 (MQ=37)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                             >  1:124003/1‑81 (MQ=255)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                             <  2:124003/81‑1 (MQ=255)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTattatt                                       <  6:296486/135‑1 (MQ=255)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTattatt                                       >  4:234174/1‑135 (MQ=255)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  1:3948/1‑136 (MQ=255)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  7:131664/1‑136 (MQ=255)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  4:394406/1‑136 (MQ=255)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      <  1:309975/136‑1 (MQ=255)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                      >  1:249558/1‑136 (MQ=255)                                                                                               ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGATTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGAAttatta                                      >  4:39286/1‑136 (MQ=255)                                                                                                 cTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCTGAGCC‑ATTGCTTCCATAGCTAAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCACGCTTGGAAGCTTATATGTATTTTTAc                                     >  5:345651/1‑135 (MQ=37)                                                                                                      aaCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAgg                                <  3:234174/135‑1 (MQ=255)                                                                                                       aCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTattatt                                       >  7:95224/1‑127 (MQ=255)                                                                                                       aCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTattatt                                       <  8:95224/127‑1 (MQ=255)                                                                                                       aCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGtt                              >  3:375356/1‑136 (MQ=255)                                                                                                          gtgcGGTCGTTGGTTAGAATCCTACCTGCGGAG‑CAATTGCTTCAATAGCTCAGCATGTAGATCACTTCCATGGGAAGGACGAGGTCAGCTGTTCGAGCCCTCTTGGAAGCTTAAATGTATTATTTCCAAGGTTtct                           <  6:46761/133‑1 (MQ=255)                                                                                                          gATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTtct                           <  7:201472/136‑1 (MQ=255)                                                                                                          gATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTtct                           <  2:59037/136‑1 (MQ=255)                                                                                                              ggTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                        >  8:269828/1‑135 (MQ=255)                                                                                                              ggTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                        >  6:36279/1‑135 (MQ=255)                                                                                                              ggTCGTAGGTTCGAATCCTACATGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                        >  5:139987/1‑135 (MQ=255)                                                                                                                 cGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAgg                    <  8:146139/136‑1 (MQ=255)                                                                                                                 cGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAgg                    >  8:152890/1‑136 (MQ=255)                                                                                                                 cGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAgg                    >  8:219049/1‑136 (MQ=255)                                                                                                                 cGTAGGGTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAgg                    <  5:288387/136‑1 (MQ=255)                                                                                                                   tAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgag                  <  3:45206/136‑1 (MQ=255)                                                                                                                             tCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                >  2:201535/1‑48 (MQ=37)                                                                                                                             tCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                <  1:201535/48‑1 (MQ=37)                                                                                                                             tCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAATCttttt        >  8:329920/1‑136 (MQ=255)                                                                                                                             tCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCttttt        >  1:161267/1‑136 (MQ=255)                                                                                                                             tCCTACCAGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTAGGAAGCTTAAATGAATTATTACCAAGGTTTCTCTTAAGGAGAAAGCttttt        >  4:284430/1‑136 (MQ=255)                                                                                                                                   cTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTg  <  8:320118/136‑1 (MQ=255)                                                                                                                                           |                                                                                                                                 GAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑C‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTG  >  BS168_reference_genome/165688‑165952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑