Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 143,659 C→T S100F (TCT→TTT)  rpsE → ribosomal protein S5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome143,6590CT96.7% 304.7 / 0.6 91S100F (TCT→TTT) rpsEribosomal protein S5
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (34/54);  total (36/55)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

CCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTT  >  BS168_reference_genome/143529‑143791
                                                                                                                                  |                                                                                                                                    
ccAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAg                                                                                                                                 <  8:310051/136‑1 (MQ=255)
 cAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAgg                                                                                                                                >  5:102894/1‑136 (MQ=255)
 cAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAACTACCAATGGTTGGAACTACAATACCACACGACATCATCGGACGTTTCTGTGAAGGTAACATCTTGTTAAAACCTGCTTTAGAAgg                                                                                                                                >  5:179161/1‑136 (MQ=255)
  aGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGTCGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGt                                                                                                                               <  4:304342/136‑1 (MQ=255)
  aGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAgg                                                                                                                                >  7:339763/1‑135 (MQ=255)
  aGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGt                                                                                                                               <  6:19296/136‑1 (MQ=255)
       cGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGt                                                                                                                               >  6:298686/1‑131 (MQ=255)
       cGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGt                                                                                                                               <  5:298686/131‑1 (MQ=255)
       cGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTgg                                                                                                                          <  8:143949/136‑1 (MQ=255)
         aTTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCTGTGCAGGTAACATCTTGGTAAAACCTGCTTTTGATGGTACTGGAg                                                                                                                        >  5:263307/1‑136 (MQ=255)
            cGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTa                                                                                                                     >  3:188337/1‑136 (MQ=255)
                aaGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGc                                                                                                                 <  4:188337/136‑1 (MQ=255)
                 aGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCt                                                                                                                <  7:26096/136‑1 (MQ=255)
                 aGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCt                                                                                                                <  1:151824/136‑1 (MQ=255)
                   cTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTgg                                                                                                              <  1:247189/136‑1 (MQ=255)
                      ttGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAgg                                                                                                           <  4:144782/136‑1 (MQ=255)
                      ttGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAgg                                                                                                           <  3:152098/136‑1 (MQ=255)
                       tGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGc                                                                                                          >  3:93307/1‑136 (MQ=255)
                         aaGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGATTCGGTGCAGGTAACATCTTGTTAAAAACTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGccc                                                                                                        >  1:322780/1‑136 (MQ=255)
                          aGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCt                                                                                                       >  4:294897/1‑136 (MQ=255)
                              gCGAAAAAGAATTTGATTGAAGTACCAAAGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTAc                                                                                                   >  4:230476/1‑136 (MQ=255)
                               cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTAc                                                                                                   <  4:48083/135‑1 (MQ=255)
                               cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTAc                                                                                                   <  3:292272/135‑1 (MQ=255)
                               cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTAc                                                                                                   <  7:276544/135‑1 (MQ=255)
                               cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACg                                                                                                  <  2:335839/136‑1 (MQ=255)
                                gAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                 >  4:146513/1‑136 (MQ=255)
                                gAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                 <  1:109836/136‑1 (MQ=255)
                                gAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                 <  5:208771/136‑1 (MQ=255)
                                gAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                 >  1:344971/1‑136 (MQ=255)
                                   aaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCg                                                                                              >  6:68072/1‑136 (MQ=255)
                                   aaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCg                                                                                              >  3:158775/1‑136 (MQ=255)
                                    aaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCgg                                                                                             <  8:339763/136‑1 (MQ=255)
                                    aaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCgg                                                                                             <  4:221790/136‑1 (MQ=255)
                                         tttGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACtt                                                                                        <  3:256526/136‑1 (MQ=255)
                                            gattgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGag                                                                                     >  7:288897/1‑136 (MQ=255)
                                                 aaGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagctagc                                                                                >  1:315124/1‑136 (MQ=255)
                                                    tACCAATGGTTTGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCACTGGAGGACCTGTACGTGCGGTACTTGAGCTAGCTgg                                                                             <  7:284123/136‑1 (MQ=255)
                                                    tACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                 >  3:193257/1‑116 (MQ=255)
                                                    tACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                 <  4:193257/116‑1 (MQ=255)
                                                    tACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTgg                                                                             >  2:363521/1‑136 (MQ=255)
                                                         aTGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAg                                                                        >  8:213429/1‑136 (MQ=255)
                                                         aTGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAg                                                                        <  8:288897/136‑1 (MQ=255)
                                                          tGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGc                                                                       <  2:344971/136‑1 (MQ=255)
                                                            gTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTg                                                                     <  1:359100/136‑1 (MQ=255)
                                                               ggAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGt                                                                                                                       >  6:273207/1‑83 (MQ=255)
                                                               ggAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGt                                                                                                                       <  5:273207/83‑1 (MQ=255)
                                                               ggAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGat                                                                   <  6:80864/135‑1 (MQ=255)
                                                                 aaCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATc                                                                >  1:392749/1‑136 (MQ=255)
                                                                 aaCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGAACGTGCGGTACTTGagct                                                                                   <  2:133782/117‑1 (MQ=255)
                                                                 aaCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGAACGTGCGGTACTTGagct                                                                                   >  1:133782/1‑117 (MQ=255)
                                                                    tACACTTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCtt                                                             <  4:226694/136‑1 (MQ=255)
                                                                      cAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCttt                                                            <  8:153066/135‑1 (MQ=255)
                                                                      cAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCtt                                                             <  3:294897/134‑1 (MQ=255)
                                                                      cAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCtt                                                             <  4:359671/134‑1 (MQ=255)
                                                                      cAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCGTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCtt                                                             <  5:264825/134‑1 (MQ=255)
                                                                      cAATTACACACGAATTCATATGTCGTTTCGGTTCAGGTATCTTCTTGTTAAAACCTTCTTTTGAATGTACTTGAGTTATCGCTGGAGTCCGTGTACGTTCGTTACTTTAGCTATCTGGTGTAGCTGATATCCtt                                                             <  4:107293/134‑1 (MQ=255)
                                                                          tCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAg                                                       >  4:166706/1‑136 (MQ=255)
                                                                            cacaCGAAATCATCTGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGt                                                      <  3:405258/135‑1 (MQ=255)
                                                                            cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGt                                                      <  6:69393/135‑1 (MQ=255)
                                                                            cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGt                                                      <  5:361738/135‑1 (MQ=255)
                                                                            cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTc                                                     <  7:120944/136‑1 (MQ=255)
                                                                            cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACATGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTAAGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTc                                                     >  5:229035/1‑136 (MQ=255)
                                                                            cacaCGAAATCATCGGACGGTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATAGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGt                                                      <  4:76272/135‑1 (MQ=255)
                                                                              cacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTAc                                                                                                   <  1:110458/88‑1 (MQ=255)
                                                                              cacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTAc                                                                                                   >  2:110458/1‑88 (MQ=255)
                                                                              cacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                   >  5:152654/1‑136 (MQ=255)
                                                                              cacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                   >  7:117253/1‑136 (MQ=255)
                                                                              cacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                   >  3:209257/1‑136 (MQ=255)
                                                                               acGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCttt                                                  <  5:68072/136‑1 (MQ=255)
                                                                                 gAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCtt                                                             >  6:212440/1‑123 (MQ=255)
                                                                                 gAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCtt                                                             <  5:212440/123‑1 (MQ=255)
                                                                                     tcatcGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTc                                            <  2:315124/136‑1 (MQ=255)
                                                                                      catcCGACGTTTCGGTGCAGGTATCATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTCTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTACTAAGTCTTTAGGATc                                            >  7:278280/1‑135 (MQ=255)
                                                                                           gACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAaca                                       >  7:315730/1‑135 (MQ=255)
                                                                                            aCGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAacaca                                     <  3:40927/136‑1 (MQ=255)
                                                                                             cGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAacacac                                    <  6:152654/136‑1 (MQ=255)
                                                                                                    gTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCaa                             >  6:171803/1‑136 (MQ=255)
                                                                                                       cAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACAt                          <  1:363521/136‑1 (MQ=255)
                                                                                                             aCATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCg                    >  3:287190/1‑136 (MQ=255)
                                                                                                             aCATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCg                    >  3:404716/1‑136 (MQ=255)
                                                                                                               aTCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTg                  <  7:213429/136‑1 (MQ=255)
                                                                                                               aTCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTg                  <  3:411205/136‑1 (MQ=255)
                                                                                                               aTCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTg                  <  4:404716/136‑1 (MQ=255)
                                                                                                               aTCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTg                  <  1:412242/136‑1 (MQ=255)
                                                                                                                  ttGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCttt                                                  >  2:251670/1‑101 (MQ=255)
                                                                                                                  ttGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCttt                                                  <  1:251670/101‑1 (MQ=255)
                                                                                                                  ttGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCaa               >  7:64751/1‑136 (MQ=255)
                                                                                                                     ttAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAaca             <  3:235960/135‑1 (MQ=255)
                                                                                                                           ccTGCTTTTGAAGGTTCTGGAGTTATCGAGGGTGGCCGTGTAAGTGCGGTAATTGAGCTAGCTGGTGTAGCTGATATCCTATCTAATTATTTAGGTTCTAACAAACCGAGCATCCTGATTCGTGCAACACTTGAAg      <  6:263307/136‑1 (MQ=255)
                                                                                                                               cTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGttt  <  1:343992/136‑1 (MQ=255)
                                                                                                                               cTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGttt  <  8:64751/136‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                    
CCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTT  >  BS168_reference_genome/143529‑143791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑