BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	1,317,158	(T)_5→6	coding (257/258 nt)	yjpA ←	putative enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,317,153	1	.	T	90.1%	474.7 / 46.4	142	intergenic (+24/+4)	phrA/yjpA	secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
Reads supporting (aligned to +/- strand): ref base . (10/4); new base T (42/86); total (52/90)
Fisher's exact test for biased strand distribution p-value = 7.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01

Only 100 of 146 total aligned reads displayed.

GATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GG‑‑C‑T‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCG  >  BS168_reference_genome/1317009‑1317286

gATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCCTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GG‑‑G‑t‑c‑ttt                                                                                                                                   <  1:2020815/150‑1 (MQ=255)

   gTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GG‑‑G‑T‑C‑ttt‑ttt                                                                                                                               <  2:931616/150‑1 (MQ=255)

     cAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GG‑‑G‑T‑C‑ttt‑tttat                                                                                                                             <  2:3362753/150‑3 (MQ=255)

      aGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACTTGGTGCATAAAAAAAGACCCTTAG‑GG‑‑G‑T‑C‑ttt‑tttatt                                                                                                                            >  1:1294559/1‑147 (MQ=255)

        gTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCCGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GG‑‑G‑T‑C‑ttt‑tttatttc                                                                                                                          >  1:30480/1‑145 (MQ=255)

          ttgttgCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATttctt                                                                                                                           <  1:2828977/150‑1 (MQ=255)

            tttgCTCGTTCCGGCCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCa                                                                                                                         <  2:1180737/149‑1 (MQ=255)

            gttgCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCa                                                                                                                         <  2:3851512/150‑1 (MQ=255)

               gCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCt                                                                                                                      <  2:3513392/150‑1 (MQ=255)

                 tCGTTGCGGTCGGGTCCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCtac                                                                                                                    <  2:360325/150‑3 (MQ=255)

                 tCGTTGCGGTCGGGTCCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTc                                                                                                                    <  1:686571/150‑1 (MQ=255)

                  cGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTcc                                                                                                                   <  2:3276049/150‑1 (MQ=255)

                   gTTGCGGTCGGGTTCAGCTTTCCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCa                                                                                                                  <  2:3021081/150‑1 (MQ=255)

                   gTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCa                                                                                                                  >  2:1538130/1‑150 (MQ=255)

                   gTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCa                                                                                                                  <  1:2470715/150‑1 (MQ=255)

                    ttGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCAt                                                                                                                 <  1:3506713/150‑1 (MQ=255)

                    ttGCGGGCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCAt                                                                                                                 <  1:4131283/150‑1 (MQ=255)

                    tggCGGTCGGGTTCAGCTTTCCGCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCAt                                                                                                                 <  2:1844969/148‑1 (MQ=255)

                      gCGGTCGGGTTCAGCTTCACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTC‑T‑TGTTT‑ATTTCTTCAGCTTCCATTc                                                                                                               >  2:2064343/1‑150 (MQ=255)

                       cGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTCCAGCTTCCATTCt                                                                                                              >  1:145076/1‑150 (MQ=255)

                        ggacGGGTTCAGCTTTACGAAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCtt                                                                                                             <  1:1268854/147‑1 (MQ=255)

                        ggTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCtt                                                                                                             >  2:1483227/1‑150 (MQ=255)

                        ggTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCtt                                                                                                             <  2:2962603/150‑1 (MQ=255)

                             ggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACTACAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAATAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATc                                                                                                        <  1:2235186/150‑1 (MQ=255)

                             ggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATc                                                                                                        <  2:1240015/150‑1 (MQ=255)

                              gTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCg                                                                                                       >  1:2090121/1‑150 (MQ=255)

                               ttCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                                      <  2:1344183/150‑1 (MQ=255)

                               ttCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                                      <  2:3122618/150‑1 (MQ=255)

                                  aGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTC‑TGT‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAg                                                                                                   >  1:4058606/1‑150 (MQ=255)

                                     tttACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATGAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc                                                                                                <  2:2728166/150‑1 (MQ=255)

                                      ttACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCa                                                                                               >  2:1340596/1‑150 (MQ=255)

                                        tcTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCaga                                                                                             <  2:69413/149‑1 (MQ=255)

                                         cTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCagaa                                                                                            <  2:3361771/150‑1 (MQ=255)

                                          tCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCagaag                                                                                           >  1:1816682/1‑150 (MQ=255)

                                            aGGTGATGGTCCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGCCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGAt                                                                                         <  1:2678572/150‑1 (MQ=255)

                                              gTGATGGTACATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCTTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATcc                                                                                       <  1:1874891/150‑1 (MQ=255)

                                                gATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAACCATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCAc                                                                                     <  2:3427137/150‑1 (MQ=255)

                                                gATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGGAGATCCAc                                                                                     >  1:4250539/1‑150 (MQ=255)

                                                gATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGGAGATCCAc                                                                                     >  1:1025993/1‑150 (MQ=255)

                                                 aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACt                                                                                    <  2:2568963/150‑1 (MQ=255)

                                                  tGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACtt                                                                                   <  1:3492104/150‑1 (MQ=255)

                                                    gTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGc                                                                                 >  1:620846/1‑150 (MQ=255)

                                                         tGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGccacca                                                                            <  1:3222566/150‑1 (MQ=255)

                                                           cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGc                                                                          <  2:3781811/150‑1 (MQ=255)

                                                             ggTGAAACAGCAACCACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCgg                                                                        <  2:1094092/150‑1 (MQ=255)

                                                               tGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAGAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGAt                                                                      <  1:3729491/150‑1 (MQ=255)

                                                                  aaCAGCAAACACGGAAGGGAAAACATTTCCTATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCg                                                                   <  2:643885/150‑1 (MQ=255)

                                                                  aaCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCg                                                                   <  2:18521/150‑1 (MQ=255)

                                                                    cAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGGTCCACTTGCCACCAGCGGATCCGCa                                                                 <  1:1209065/150‑1 (MQ=255)

                                                                    cAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa                                                                 <  1:4545791/150‑1 (MQ=255)

                                                                    cAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCCCAGAAGATCCTCTTGCCACCAGCGGATCCGCa                                                                 <  1:4670353/150‑1 (MQ=255)

                                                                      gCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGAGCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATg                                                               <  1:389127/150‑1 (MQ=255)

                                                                      gCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTC‑T‑T‑TTTCATTTCTTCAGATCCCATTCTTTTATCCTCAGCTCTGAAGATCCACTTGCCACCTGCGGATCCGCATg                                                               >  2:2910994/1‑150 (MQ=255)

                                                                        aaccacaGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGc                                                             <  2:1255166/147‑1 (MQ=255)

                                                                        aaaCACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGc                                                             >  1:2221308/1‑150 (MQ=255)

                                                                         aaCACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCTTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGcc                                                            <  1:2477793/150‑1 (MQ=255)

                                                                         aaCACAGAAGGGAAAACATTGCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATCCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGcc                                                            <  2:4704320/150‑1 (MQ=255)

                                                                             caGAAGGGAAACCATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGAtt                                                        <  2:4393399/150‑1 (MQ=255)

                                                                             caGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGCCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGAtt                                                        <  1:4810702/150‑1 (MQ=255)

                                                                               gAAGGGAAAACATTTCATATTGCGGCAGGCAACCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTc                                                      <  2:3216544/150‑1 (MQ=255)

                                                                               gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGGGGATCCGCCTGGCCGATTTc                                                      >  1:698192/1‑150 (MQ=255)

                                                                               gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTc                                                      >  2:3855646/1‑150 (MQ=255)

                                                                               gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTc                                                      <  1:1421172/150‑1 (MQ=255)

                                                                               gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTC‑T‑TATTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTc                                                      >  1:2229109/1‑150 (MQ=255)

                                                                                   ggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACTCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                                  <  2:3604656/150‑1 (MQ=255)

                                                                                   ggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTTTCGTCAGCTCAGAAGCTCCACTTGCCACCAGCGGATCCGCATGGCCGAATTCCGCt                                                  >  1:1806976/1‑150 (MQ=255)

                                                                                   ggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                                  >  2:2861189/1‑150 (MQ=255)

                                                                                   ggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                                  <  1:2842438/150‑1 (MQ=255)

                                                                                   ggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                                  >  2:4096592/1‑150 (MQ=255)

                                                                                       aaCATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCct                                              >  2:9548/1‑150 (MQ=255)

                                                                                          aTTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCtt                                           <  1:3136276/150‑1 (MQ=255)

                                                                                            ttCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCCCCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTcc                                         >  1:691605/1‑150 (MQ=255)

                                                                                             tCATATTGCGGCACGCAATCAAACATGATGCATAACAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTCCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCa                                        <  2:433796/150‑1 (MQ=255)

                                                                                              cATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAg                                       <  1:4337059/150‑1 (MQ=255)

                                                                                               atatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGt                                      >  2:3969165/1‑150 (MQ=255)

                                                                                               atatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGt                                      >  2:3804294/1‑150 (MQ=255)

                                                                                                tatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGGTTTCCGCTGCCTCTTCCAGTg                                     >  1:3224286/1‑150 (MQ=255)

                                                                                                 atTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGa                                    >  2:4169911/1‑150 (MQ=255)

                                                                                                 atTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGa                                    <  2:352092/150‑1 (MQ=255)

                                                                                                       gCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGc                              <  2:3166985/150‑1 (MQ=255)

                                                                                                        catgCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCt                             <  2:4790911/147‑1 (MQ=255)

                                                                                                         aCGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCtt                            <  2:2224324/150‑1 (MQ=255)

                                                                                                          cGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTc                           >  1:4788839/1‑150 (MQ=255)

                                                                                                          cGCAAACAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑TTTTCTTCCGCTTCCATTCTTTTACCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTc                           <  1:4148636/150‑1 (MQ=255)

                                                                                                           gCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg                          >  2:3183474/1‑150 (MQ=255)

                                                                                                           gCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCg                          >  1:2367631/1‑150 (MQ=255)

                                                                                                            cAATCAAACATGGTGCATAAAAAAAGACCCTTAGAGGT‑CTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATGCACCTTCCATCAGCTGGTCCGCATGGTCGGTATCCGCTGCCTCTTCCAGTGGATCTGCTTTGa                         >  1:4343258/1‑150 (MQ=255)

                                                                                                            cAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCGACTTGCGACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGa                         >  1:1138364/1‑150 (MQ=255)

                                                                                                             aaTCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt                        <  1:216125/150‑1 (MQ=255)

                                                                                                              aTCAAACATGATGCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATg                       >  2:3922800/1‑150 (MQ=255)

                                                                                                                        atgCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTc             <  1:2649686/150‑1 (MQ=255)

                                                                                                                         tgCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTcc            <  2:3908254/150‑1 (MQ=255)

                                                                                                                          gCATAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCg           >  1:80748/1‑150 (MQ=255)

                                                                                                                            aTAAAAAAAGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTCCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGcc         <  1:2341816/150‑1 (MQ=255)

                                                                                                                               aaaaaaGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCtgt      <  2:2329934/150‑1 (MQ=255)

                                                                                                                                aaaaaGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGCTGACATACGCTCCGCCtgtg     >  2:347903/1‑150 (MQ=255)

                                                                                                                                aaaaaGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCtggg     >  2:3064096/1‑148 (MQ=255)

                                                                                                                                 aaaaGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTgg    >  2:2391103/1‑150 (MQ=255)

                                                                                                                                  aaaGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCCATGACATACGCTCCGCCTGTGGc   >  1:3375517/1‑150 (MQ=255)

                                                                                                                                   aaGACCCTTAG‑GGGTCTT‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGACCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCg  <  2:1000216/150‑1 (MQ=255)

GATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAG‑GG‑‑C‑T‑T‑TTT‑ATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCG  >  BS168_reference_genome/1317009‑1317286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`