BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	3,770,066	(A)_8→9	intergenic (‑258/+38)	ywzF ← / ← csbD	conserved protein of unknown function/stress response protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	3,770,058	1	.	A	95.5%	747.0 / 24.3	201	intergenic (‑250/+46)	ywzF/csbD	conserved protein of unknown function/stress response protein
Reads supporting (aligned to +/- strand): ref base . (4/5); new base A (88/104); total (92/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

GATTTCTTCAAATGATGTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑AAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGCATGTCCGTTC  >  BS168_reference_genome/3769910‑3770201

 aTTTCTTCAAATGATGTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑aa                                                                                                                                                >  1:281971/1‑150 (MQ=255)

  tttCTTCAAATGATGTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTACTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑aaa                                                                                                                                               <  1:2127089/150‑1 (MQ=255)

     cttcAAATGATGTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑aaaaaa                                                                                                                                            <  1:2304615/150‑1 (MQ=255)

       tcAAATGATGTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑aaaaaa‑aa                                                                                                                                         <  2:3585182/150‑1 (MQ=255)

        cAAATGATGTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑aaaaaa‑aaa                                                                                                                                        <  1:2694685/150‑2 (MQ=255)

            tgatgTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑aaaaaa‑aaagaca                                                                                                                                    <  1:4434925/150‑6 (MQ=255)

             gatgTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACaa                                                                                                                                    <  1:63194/150‑1 (MQ=255)

             gatgTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACaa                                                                                                                                    <  2:1684959/150‑1 (MQ=255)

              atgTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAg                                                                                                                                   <  1:991550/150‑1 (MQ=255)

              atgTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAg                                                                                                                                   <  1:3745638/150‑1 (MQ=255)

               tgTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGa                                                                                                                                  >  1:3013032/1‑150 (MQ=255)

                gTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGaa                                                                                                                                 <  2:638376/150‑1 (MQ=255)

                 ttATGAAGGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGaaa                                                                                                                                <  2:3270164/150‑1 (MQ=255)

                  tataAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAAc                                                                                                                               <  1:404161/150‑1 (MQ=255)

                      aaaGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACAttt                                                                                                                           <  2:1512269/150‑1 (MQ=255)

                       aaGTTTCCAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTc                                                                                                                          <  2:565419/150‑1 (MQ=255)

                        aGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑AAAAAA‑AAGACAAGAAACATTTCGt                                                                                                                        <  2:2661823/150‑1 (MQ=255)

                         gTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGt                                                                                                                        >  2:2319331/1‑150 (MQ=255)

                           ttACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTTTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACACTTCGttt                                                                                                                      >  1:412557/1‑150 (MQ=255)

                              cAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAAATTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GG‑G‑AAAAAA‑AAGACAAGAAACATTTCGTTTCCTg                                                                                                                  >  1:2915693/1‑150 (MQ=255)

                               aaCTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTg                                                                                                                  >  2:367246/1‑150 (MQ=255)

                                aCTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGc                                                                                                                 >  1:3101317/1‑150 (MQ=255)

                                      gTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTc                                                                                                           <  1:2919371/150‑1 (MQ=255)

                                        catcatTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCg                                                                                                         <  1:2254373/150‑1 (MQ=255)

                                        catcatTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTAAATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCg                                                                                                         >  1:3790456/1‑150 (MQ=255)

                                         atcatTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGt                                                                                                        >  2:2459311/1‑150 (MQ=255)

                                           catTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGTCCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATCTCGTTTCCTGCCTCTTCCGTAc                                                                                                      >  1:2003804/1‑150 (MQ=255)

                                              tatataGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTACTTTTTTTGTTATATTTATTCACATCAGCAAAT‑GCTA‑AAAAAA‑AAGACAAGAAACATTTCGTTTCCTCCCTCTTCCGTACGAt                                                                                                   >  1:1595161/1‑150 (MQ=255)

                                               atataGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGAtt                                                                                                  <  1:407021/150‑1 (MQ=255)

                                                 ataGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTaa                                                                                                >  2:4733755/1‑150 (MQ=255)

                                                 ataGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTaa                                                                                                <  1:3107404/150‑1 (MQ=255)

                                                 ataGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTaa                                                                                                <  1:4687060/150‑1 (MQ=255)

                                                    gaagaGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCt                                                                                             <  2:2028745/150‑1 (MQ=255)

                                                     aagaGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCtt                                                                                            <  1:3303589/150‑1 (MQ=255)

                                                     aagaGGCCTATTAATACAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCtt                                                                                            <  2:2106023/150‑1 (MQ=255)

                                                      agagGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCttt                                                                                           <  2:227440/150‑1 (MQ=255)

                                                       gagGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTa                                                                                          >  1:3553280/1‑150 (MQ=255)

                                                        agGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTAt                                                                                         <  2:4413284/150‑1 (MQ=255)

                                                          gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCt                                                                                       >  2:4474455/1‑150 (MQ=255)

                                                          gCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATGAATCTTTATCt                                                                                       >  1:970299/1‑150 (MQ=255)

                                                           ccTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTAATCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTg                                                                                      >  1:1873073/1‑150 (MQ=255)

                                                             tATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGaa                                                                                    <  2:500768/150‑1 (MQ=255)

                                                             tATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGaa                                                                                    >  1:452664/1‑150 (MQ=255)

                                                              aTTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGgaa                                                                                   >  1:4351668/1‑150 (MQ=255)

                                                              aTTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACCATTAATCTTTATCTGaaa                                                                                   >  1:2186940/1‑150 (MQ=255)

                                                                tAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCATATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAAt                                                                                 >  1:2775368/1‑150 (MQ=255)

                                                                tAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAAt                                                                                 <  2:3843213/150‑1 (MQ=255)

                                                                 aaTTCAAAGAATATTTAAAGAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAAtt                                                                                <  1:1745825/150‑1 (MQ=255)

                                                                   ttCAAAGAGTATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTa                                                                              <  2:3162478/150‑1 (MQ=255)

                                                                   ttCAAAGAATATTTAAAAAATTTAGTTACCTTGATCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTa                                                                              <  2:1733952/150‑1 (MQ=255)

                                                                      aaaGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATcc                                                                           <  2:4554743/150‑1 (MQ=255)

                                                                        aGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCtt                                                                         <  1:3412020/150‑1 (MQ=255)

                                                                          aaTATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTgg                                                                       >  1:2622482/1‑150 (MQ=255)

                                                                             atTTAAAAAATTTAGTTACCTTGTCCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCtt                                                                    >  1:1299415/1‑150 (MQ=255)

                                                                                 aaaaaaTTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTccc                                                                <  1:4474455/150‑1 (MQ=255)

                                                                                  aaaaaTTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCg                                                               <  2:3176020/150‑1 (MQ=255)

                                                                                  aaaaaTTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCg                                                               <  2:672023/150‑1 (MQ=255)

                                                                                  aaaaaTTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCg                                                               >  1:2078742/1‑150 (MQ=255)

                                                                                  aaaaaTTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCg                                                               <  1:2309178/150‑1 (MQ=255)

                                                                                     aaTTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGTCTTTCCCGata                                                            >  1:583607/1‑150 (MQ=255)

                                                                                      aTTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGatat                                                           <  2:4646270/150‑1 (MQ=255)

                                                                                       tttAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATc                                                          <  2:3091563/150‑1 (MQ=255)

                                                                                         tAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCt                                                        >  1:1096817/1‑150 (MQ=255)

                                                                                         tAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCt                                                        <  2:3013032/150‑1 (MQ=255)

                                                                                           gTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGTAAAA‑AC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAAATTATCCTTGGCTTTCCCGATATCCttt                                                      >  1:1155419/1‑150 (MQ=255)

                                                                                           gTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGGAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCttt                                                      >  1:258800/1‑150 (MQ=255)

                                                                                            ttACCTTGTCCAAATCAGCTTCTTTTTTTGTTATATTTATACACATAAGAAAAAGGT‑A‑AAAAAA‑AAGACAAGAAAAATTTCGTTTCCTGCCTCTTCCGTACGATTATTCTTTTTCTGAAAATTAATCCTTGGCTTTCCCGCTATCCtttt                                                     >  1:335418/1‑150 (MQ=255)

                                                                                               ccTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGa                                                  <  1:498401/150‑1 (MQ=255)

                                                                                               ccTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCCATATCCTTTTGGa                                                  >  1:3267275/1‑150 (MQ=255)

                                                                                                cTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGAt                                                 <  2:1537546/150‑1 (MQ=255)

                                                                                                 ttGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGAtt                                                <  1:1329077/150‑1 (MQ=255)

                                                                                                 ttGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGAtt                                                <  2:2132405/150‑1 (MQ=255)

                                                                                                  tGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGAttt                                               <  1:3047799/150‑1 (MQ=255)

                                                                                                     ccAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGc                                            <  2:2775368/150‑1 (MQ=255)

                                                                                                      cAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGcc                                           <  1:2372870/150‑1 (MQ=255)

                                                                                                        aaTCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCTTGCCTCTTCCGTACGATTAATCTTTATCTTAAAATTTATCCTTGGCTTTCCCGCTATCCTTTTGGTTTTCGCCtt                                         >  1:731521/1‑150 (MQ=255)

                                                                                                        aaTCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑AAAAAACAAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCtt                                         <  2:1246223/150‑1 (MQ=255)

                                                                                                                  tttttttGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCCATATCCTTTTGGATTTCGCCTTTTGCCTTATc                               <  1:3821990/150‑1 (MQ=255)

                                                                                                                   ttttttGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTTCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCt                              <  1:360393/150‑1 (MQ=255)

                                                                                                                   ttttttGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGGAAATTTATCCTTGGCCTTCCCGATATCCTCTTGAATTTCCCCTTTTGCCTTATCt                              >  1:1392871/1‑150 (MQ=255)

                                                                                                                    tttttGTTGTATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGACACATTTCATTTCCTGCCTCTTCCGTTCGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCtt                             <  2:1365405/150‑1 (MQ=255)

                                                                                                                    tttttGTTATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGGTATCCTTTTGGATTTTGCCTTTTGCCTTATCtt                             >  1:4637832/1‑150 (MQ=255)

                                                                                                                          ttATATTTATTCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTTATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGc                       >  1:370757/1‑150 (MQ=255)

                                                                                                                               tttattCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCa                  >  1:1947306/1‑150 (MQ=255)

                                                                                                                               tttattCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCa                  >  1:2273934/1‑150 (MQ=255)

                                                                                                                                ttattCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAg                 <  1:425617/150‑1 (MQ=255)

                                                                                                                                 tattCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGc                <  1:4874188/150‑1 (MQ=255)

                                                                                                                                 cattCACATCAGAAAAA‑GC‑G‑AAAAAAGAAGACAAGAAACATTTCGTTTCCTGCCTCATCCGTACGATTAATCTTTATCTGAAAATTTAGCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGc                <  1:865991/149‑1 (MQ=255)

                                                                                                                                   ttCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCTTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCt              >  1:3691419/1‑150 (MQ=255)

                                                                                                                                   ttCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCt              <  2:3142358/150‑1 (MQ=255)

                                                                                                                                    tCACATCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTCTTGCCTTCAGCCtc             >  2:2480043/1‑149 (MQ=255)

                                                                                                                                     cacaTCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACTTTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGc            <  1:2540504/150‑1 (MQ=255)

                                                                                                                                     cacaTCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGc            >  2:1169954/1‑150 (MQ=255)

                                                                                                                                      acaTCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATTATTTTTGCCTTCTGCCTGCa           >  2:158934/1‑150 (MQ=255)

                                                                                                                                      acaTCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGCa           <  2:3716815/150‑1 (MQ=255)

                                                                                                                                       caTCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAATCATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGATAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGCAt          <  2:346625/150‑1 (MQ=255)

                                                                                                                                       caTCAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGCAt          <  2:2200700/150‑1 (MQ=255)

                                                                                                                                          cAGAAAAA‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGCATGTc       >  1:1315646/1‑150 (MQ=255)

                                                                                                                                               aaa‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGTAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGCATGTCCGTTc  >  1:3171830/1‑150 (MQ=255)

                                                                                                                                               aaa‑GC‑GAAAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGCATGTCCGTTc  <  2:412557/150‑1 (MQ=255)

GATTTCTTCAAATGATGTTATAAAAGTTTACAACTTATGTCATCATTATATAGAAGAGGCCTATTAATTCAAAGAATATTTAAAAAATTTAGTTACCTTGACCAAATCAGCTTCTTTTTTTGTTATATTTATTCACATCAGAAAAA‑GC‑G‑AAAAAA‑AAGACAAGAAACATTTCGTTTCCTGCCTCTTCCGTACGATTAATCTTTATCTGAAAATTTATCCTTGGCTTTCCCGATATCCTTTTGGATTTCGCCTTTTGCCTTATCTTTTTTGCCTTCAGCCTGCATGTCCGTTC  >  BS168_reference_genome/3769910‑3770201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`