BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	4,155,395	(A)_5→6	intergenic (‑186/+38)	trnY‑Lys ← / ← purA	tRNA‑Lys/adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	4,155,390	1	.	A	94.2%	1829.0 / 97.6	516	intergenic (‑181/+43)	trnY‑Lys/purA	tRNA‑Lys/adenylosuccinate synthetase
Reads supporting (aligned to +/- strand): ref base . (7/23); new base A (244/242); total (251/265)
Fisher's exact test for biased strand distribution p-value = 4.46e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 568 total aligned reads displayed.

GACTTAAATCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑C‑AAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCTGAGACACACGCTCA  >  BS168_reference_genome/4155241‑4155535

gACTTAAATCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaa‑c                                                                                                                                                     >  1:3427902/1‑150 (MQ=255)

gACTTAAATCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaa‑c                                                                                                                                                     >  1:4258062/1‑150 (MQ=255)

gACTTAAATCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaa‑c                                                                                                                                                     >  2:1841292/1‑150 (MQ=255)

gACTTAAATCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaa‑c                                                                                                                                                     <  1:3628669/150‑1 (MQ=255)

 aCTTAAATCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaa‑c‑a                                                                                                                                                   >  2:1381135/1‑150 (MQ=255)

    tAAATCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaa‑c‑aaaa                                                                                                                                                >  1:4320830/1‑150 (MQ=255)

     aaaTCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaa‑c‑aaaaa                                                                                                                                               >  2:1298353/1‑150 (MQ=255)

       aTCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGGGGCTTTC‑aaa‑c‑aaaaaa                                                                                                                                              >  2:236162/1‑149 (MQ=255)

       aTCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaa‑c‑aaaaaac                                                                                                                                             >  2:1561385/1‑148 (MQ=255)

        tCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaa‑c‑aaaaaaca                                                                                                                                            >  1:352381/1‑147 (MQ=255)

          tAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaa‑c‑aaaaaacaag                                                                                                                                          >  1:2742727/1‑145 (MQ=255)

           aGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGc                                                                                                                                         >  2:3943850/1‑150 (MQ=255)

            gTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGcc                                                                                                                                        >  2:1916041/1‑150 (MQ=255)

            gTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGcc                                                                                                                                        <  1:4379751/150‑1 (MQ=255)

              tctcATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGcccc                                                                                                                                      <  1:3727664/150‑1 (MQ=255)

              tctcATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGcccc                                                                                                                                      <  1:3285999/150‑1 (MQ=255)

               ctcATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGccccc                                                                                                                                     >  2:2043191/1‑149 (MQ=255)

                tcATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAC‑CAAAAAA‑CAAGCCCCtt                                                                                                                                    >  1:4609602/1‑150 (MQ=255)

                 cATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTa                                                                                                                                   <  2:2518992/150‑1 (MQ=255)

                 cATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTa                                                                                                                                   >  2:48852/1‑150 (MQ=255)

                   tCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATGTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTaaa                                                                                                                                 >  2:2829487/1‑150 (MQ=255)

                    cTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAAt                                                                                                                                >  2:4514522/1‑150 (MQ=255)

                    cTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAAt                                                                                                                                <  1:1329709/150‑1 (MQ=255)

                     ttGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCTGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATa                                                                                                                               >  2:4277435/1‑150 (MQ=255)

                         aCTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGGTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAgggg                                                                                                                           >  2:2113549/1‑150 (MQ=255)

                            ctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGcct                                                                                                                        >  2:3346942/1‑148 (MQ=255)

                              aTTGAAGCAGGTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTGTCAGACCAACTCATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGc                                                                                                                      >  1:754099/1‑150 (MQ=255)

                               ttGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCa                                                                                                                     >  1:3246019/1‑150 (MQ=255)

                                  aaGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                                  <  1:3937304/150‑1 (MQ=255)

                                  aaGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCCTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                                  >  2:4132756/1‑150 (MQ=255)

                                    gCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACat                                                                                                                <  1:1986993/150‑1 (MQ=255)

                                    gCAGTTTCCGTAACAACGAGATTTAAAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACat                                                                                                                <  1:827902/150‑1 (MQ=255)

                                      aGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGATGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACatat                                                                                                              <  1:3352733/150‑1 (MQ=255)

                                      aGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACatat                                                                                                              >  1:4794365/1‑150 (MQ=255)

                                       gTTTCCGAAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATAtt                                                                                                             <  1:3872021/150‑1 (MQ=255)

                                           ccGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTAt                                                                                                         <  1:1072994/150‑1 (MQ=255)

                                            cGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTAtt                                                                                                        <  1:4095869/150‑1 (MQ=255)

                                             gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTAttt                                                                                                       >  2:4681303/1‑150 (MQ=255)

                                             gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTAttt                                                                                                       >  2:3252588/1‑150 (MQ=255)

                                              tAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGTCCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTa                                                                                                      >  2:1679876/1‑150 (MQ=255)

                                              tAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTa                                                                                                      >  1:1488569/1‑150 (MQ=255)

                                               aacaacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAg                                                                                                     >  2:122240/1‑150 (MQ=255)

                                                  aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCCCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTc                                                                                                  >  2:1512093/1‑150 (MQ=255)

                                                   acGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCg                                                                                                 <  1:1402473/150‑1 (MQ=255)

                                                   acGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTATGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCg                                                                                                 >  1:1592288/1‑150 (MQ=255)

                                                    cGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                                <  2:2214185/150‑1 (MQ=255)

                                                      agaTTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATTTTCTATTTAGTTCGCAc                                                                                              >  1:1132525/1‑150 (MQ=255)

                                                       gaTTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTAATTTGTTCGCACg                                                                                             >  2:3151206/1‑150 (MQ=255)

                                                         tttATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGt                                                                                           <  1:1855330/150‑1 (MQ=255)

                                                           tataATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTac                                                                                         >  1:984122/1‑150 (MQ=255)

                                                             taataTCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTCTCCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCACACGGTacac                                                                                       <  1:4015716/150‑1 (MQ=255)

                                                                  tCACGAACAAATAGAAGTGGCAGTATCTTTTTTGT‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑C‑AAAAACCAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGc                                                                                  <  2:2282435/150‑1 (MQ=255)

                                                                   cACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTCCAGACATATTCTATTTAGTTCGCACGGTACACACTGCg                                                                                 <  1:1782042/150‑1 (MQ=255)

                                                                    aCGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGa                                                                                <  1:3007092/150‑1 (MQ=255)

                                                                      gAACAAATGGAGGTGGCAATACCTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAg                                                                              <  2:885197/150‑1 (MQ=255)

                                                                      gAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAg                                                                              >  1:2337938/1‑150 (MQ=255)

                                                                        aCAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTAC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACAAATTCTATTTAGTTCGCACGGTACACACTGCGAAGGa                                                                            <  1:2220129/150‑1 (MQ=255)

                                                                         cAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGGCATATTCTATTTAGTTCCCACGGTACACACTGCGGAGGAc                                                                           >  2:2935696/1‑150 (MQ=255)

                                                                         cAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGAc                                                                           <  2:3427902/150‑1 (MQ=255)

                                                                         cAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTAAAGGCGGCTTTC‑AAA‑CAAAAAA‑CAAGTCTCTTAAATAGGGGCTTGCAGACATATTGTATTTAGTTCGCACGGTACACACTGCGAAGGAc                                                                           <  1:4511814/150‑1 (MQ=255)

                                                                          aaaTAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACa                                                                          <  1:2429095/150‑1 (MQ=255)

                                                                           aaTAGAAGAGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATCTTCTATTTAGTTCGCACGGTACACGCTGCGAAGGACAt                                                                         >  2:1636632/1‑150 (MQ=255)

                                                                            aTAGAGGTGGCAATATTTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTCCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtt                                                                        <  1:574329/150‑1 (MQ=255)

                                                                            aTAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtt                                                                        <  2:3315516/150‑1 (MQ=255)

                                                                             tAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAC‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAttt                                                                       <  2:4397398/150‑1 (MQ=255)

                                                                              aGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtttg                                                                      >  2:4459663/1‑150 (MQ=255)

                                                                              aGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCCCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtttg                                                                      <  1:2481160/150‑1 (MQ=255)

                                                                               gAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATCTTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtttgt                                                                     <  1:3819280/150‑1 (MQ=255)

                                                                               gAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTCTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGGAGGACAtttgt                                                                     >  1:2097019/1‑150 (MQ=255)

                                                                                aaGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtttgtt                                                                    >  1:345545/1‑150 (MQ=255)

                                                                                aaGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtttgtt                                                                    >  2:2640118/1‑150 (MQ=255)

                                                                                   tGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAAGA‑AAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGTCATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATttgtttgt                                                                 >  1:4452923/1‑150 (MQ=255)

                                                                                   tGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTTTTTAGTTCGCACGGTACACACTGCAAAGGAGATttgtttgt                                                                 <  1:665328/150‑1 (MQ=255)

                                                                                   tGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATttgtttgt                                                                 >  2:737445/1‑150 (MQ=255)

                                                                                      cAATTACTTTTTTGG‑CTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                                              <  2:1120563/150‑1 (MQ=255)

                                                                                         tAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGGCATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGGGCgg                                                           >  1:3201743/1‑150 (MQ=255)

                                                                                         tAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTCCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCgg                                                           <  1:3135836/150‑1 (MQ=255)

                                                                                         tAACTTTTTTGG‑TTTTTTTTCATATTTCTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCgg                                                           <  2:3707426/150‑1 (MQ=255)

                                                                                           aCTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTc                                                         >  1:2935004/1‑150 (MQ=255)

                                                                                           aCTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATACTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTc                                                         >  1:2524696/1‑150 (MQ=255)

                                                                                              tttttGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGGGCGGTCTgg                                                      >  2:821116/1‑150 (MQ=255)

                                                                                                tttGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGGAGGACATTTGTTTGTGAGCGGTCTGGAc                                                    >  2:4230811/1‑150 (MQ=255)

                                                                                                    g‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGTCATTTGTTTGTGGGCGGTCTGGACCGAc                                                >  1:1179400/1‑150 (MQ=255)

                                                                                                               atttttttttCTCAGACCATCTTATATGCGGCTTTC‑AAT‑C‑AAAAATCATGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATa                                      <  2:645471/147‑1 (MQ=255)

                                                                                                               atatTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATa                                      >  2:3784812/1‑150 (MQ=255)

                                                                                                                 atTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGCACATTTGTTTGTGTGCGGTCTGGGCCGGCAGGGAAAATCGa                                    >  2:1727590/1‑150 (MQ=255)

                                                                                                                        tcccAGACCACCTTATATGCGGCTTCC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGa                             <  1:2829487/147‑1 (MQ=255)

                                                                                                                          tcAGACCACCTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAAt                           <  2:4605442/150‑1 (MQ=255)

                                                                                                                          tcAGACCAACTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAAt                           <  2:3485453/150‑1 (MQ=255)

                                                                                                                                  aCTTTTCTGCGGCTTTC‑AAC‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTCCAGACATTTTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCa                   <  1:732173/150‑1 (MQ=255)

                                                                                                                                  aCTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCa                   >  2:2445361/1‑150 (MQ=255)

                                                                                                                                   cTTATATGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAg                  >  2:3610474/1‑150 (MQ=255)

                                                                                                                                    ttATATGCGGCTTCC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGc                 <  1:2635705/150‑1 (MQ=255)

                                                                                                                                    ttATATGCGGCTGTCAAAA‑A‑AAAAA‑CAAGCCCCTTAAATGGGGGCTTCCAGACATATTCTATTTAGTTCGCGCGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGc                 <  1:1924739/150‑1 (MQ=255)

                                                                                                                                     tataTGCGGCTTCC‑AAA‑C‑AAAAACCAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCt                <  2:2578422/150‑1 (MQ=255)

                                                                                                                                        atGCGGCTTTC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCTgag             <  1:1307006/150‑1 (MQ=255)

                                                                                                                                                ttC‑AAA‑CAAAAAA‑CAAGCCCCTTAAATAGGGGCTTCCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCTGAGACACACGc     <  1:4147064/150‑1 (MQ=255)

                                                                                                                                                tac‑aaa‑caaaAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCTGAGACACACGc     <  1:48852/148‑1 (MQ=255)

                                                                                                                                                  c‑aaa‑caaaAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCTGGGTCCCCCGCTc   >  2:2775767/1‑150 (MQ=255)

                                                                                                                                                    aaa‑c‑aaaaaccaAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCTGAGACACACGCTCa  <  2:304731/150‑1 (MQ=255)

GACTTAAATCTAGTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAA‑C‑AAAAA‑CAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAGCTGAGACACACGCTCA  >  BS168_reference_genome/4155241‑4155535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`