BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	166,037	+T	intergenic (+4/‑27)	trnI‑Gly → / → trnI‑Arg	tRNA‑Gly/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,037	1	.	T	99.6%	957.5 / ‑5.8	269	intergenic (+4/‑27)	trnI‑Gly/trnI‑Arg	tRNA‑Gly/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base . (0/0); major base T (143/125); minor base C (1/0); total (144/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 282 total aligned reads displayed.

GCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTC  >  BS168_reference_genome/165888‑166180

 cccGCTTGGAAGCTTAAATGTATTATTACCAAGGTGTCTCATAAGGAGAAAGCTTTTTTTGTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTat                                                                                                                                                 <  2:1457294/150‑2 (MQ=255)

    gCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTatcct                                                                                                                                              <  2:1235534/150‑5 (MQ=255)

     cTTGGAAGCTTAAATGTTTTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTGTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTatccta                                                                                                                                             <  2:1985654/150‑6 (MQ=255)

      ttGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCCCTCCATTTATCCTaa                                                                                                                                            >  2:2687128/1‑150 (MQ=255)

          aaGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGGAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACggg                                                                                                                                        >  1:3604533/1‑150 (MQ=255)

           aGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGa                                                                                                                                       >  2:2337649/1‑150 (MQ=255)

            gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGat                                                                                                                                      >  1:4293451/1‑150 (MQ=255)

            gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGat                                                                                                                                      <  2:1944988/150‑1 (MQ=255)

            gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGGCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGat                                                                                                                                      >  1:1483212/1‑150 (MQ=255)

            gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGGCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGat                                                                                                                                      >  2:1800765/1‑150 (MQ=255)

             cTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGata                                                                                                                                     <  2:394541/150‑1 (MQ=255)

              taaaaTGTATTATTACCAAGGTTTCTCATAAGGAGATAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCGGCTCCATTTATCCTAACGGGatat                                                                                                                                    <  1:531404/148‑1 (MQ=255)

                  aTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTa                                                                                                                                >  1:2040809/1‑150 (MQ=255)

                     tattatTACCAAGGTTCCTCACAAGGAGAAAGGTTTTTTTATTGCGACATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGGCAAGGTGGGGGTCGCGGGTTCGAATGCCGTCTTCCGCTCCATCTATGCTAACGGGATATTGGAaat                                                                                                                             >  1:2969272/1‑150 (MQ=18)

                       ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACCCCACCCTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTGCGCTCCATTTATCCTAACGGGATATTGTAaattg                                                                                                                           <  1:3195507/150‑1 (MQ=25)

                       ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAaattg                                                                                                                           <  2:3849539/150‑1 (MQ=255)

                         attaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAaattgaa                                                                                                                         >  1:1202732/1‑150 (MQ=255)

                             ccAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTACCCTAACGGGATATTGTAAATTGAATTgc                                                                                                                     >  1:4780108/1‑150 (MQ=255)

                               aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAGTTGAATTgcgc                                                                                                                   >  2:2881719/1‑150 (MQ=255)

                                aGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGcc                                                                                                                  <  2:1614534/150‑1 (MQ=255)

                                 ggcttCCCATAAGGAGAATGCTTTTTTTATTGCGATATGCGGAAGTAGTCCAGTGGTAGACCCCCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGccc                                                                                                                 <  1:3962829/147‑1 (MQ=25)

                                     tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                                             >  1:923611/1‑150 (MQ=255)

                                      ctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGc                                                                                                            >  1:3892288/1‑150 (MQ=255)

                                       tcATAAGGAGAAGGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                                           <  1:2550888/150‑1 (MQ=255)

                                       tcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                                           <  1:2618786/150‑1 (MQ=255)

                                       tcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAAGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                                           >  1:1276608/1‑150 (MQ=255)

                                          tAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                                        >  1:2018896/1‑150 (MQ=255)

                                             ggAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTg                                                                                                     >  1:89089/1‑150 (MQ=255)

                                              gagaATGCTTTTTTTAGTGCGATATGGGGAAGTATTTCAGTGGTAGAACACCACCTTGCCAAGGGGGGGGTCGGGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTgg                                                                                                    <  1:3390726/150‑1 (MQ=25)

                                              gagaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGGATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTgg                                                                                                    >  1:4137261/1‑150 (MQ=255)

                                                gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGAt                                                                                                  >  1:4262281/1‑150 (MQ=255)

                                                agaaGCTTTTTTTATTGCGATATGCGGTAGTAGTACAGTGGTAGAACACCACCTTGTCCAGGTGGGGGCCGCGGGTTCGAATCCCGTCCTCCGCCTGATTTATCCTAACGGGATATTGTAGATTGCATTCCGGCCGTAGCTCAATTGGAt                                                                                                  <  2:1487668/148‑1 (MQ=21)

                                                    gCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAAAGGCGCCCGTAGCTCACTTGGATagag                                                                                              >  1:3216353/1‑150 (MQ=25)

                                                     ttttttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGc                                                                                             >  2:3745442/2‑150 (MQ=255)

                                                     cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGc                                                                                             >  1:2824770/1‑150 (MQ=255)

                                                      tttttttATTGCGATATGCGGAAGTGGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCGTTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCg                                                                                            <  1:3053997/150‑1 (MQ=255)

                                                      tttttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCg                                                                                            >  1:3186037/1‑150 (MQ=255)

                                                      tttttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCg                                                                                            >  1:3540969/1‑150 (MQ=255)

                                                          tttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTg                                                                                        <  1:1839522/150‑1 (MQ=255)

                                                           ttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTgg                                                                                       >  2:1049361/1‑149 (MQ=255)

                                                            tATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTGAATTGAATTGCGCCCGTAGCTAAATTGGATAGAGCGGTTGAc                                                                                      >  1:1136212/1‑150 (MQ=25)

                                                             aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACCCCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACt                                                                                     <  2:3266227/150‑1 (MQ=255)

                                                              ttGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGAGATAATGGAAATTGAATTGCGCCCGTAGCTCAATTGGATAGGGCCTTTGACTa                                                                                    >  1:3118480/1‑150 (MQ=21)

                                                                gCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACg                                                                                  <  1:160256/150‑1 (MQ=255)

                                                                gCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACg                                                                                  >  2:388374/1‑150 (MQ=255)

                                                                  gATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGGCTACGGa                                                                                >  2:3336932/1‑150 (MQ=255)

                                                                     atGCGGAAGTAGTTCAGTGGTAGAACCCCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCa                                                                             <  2:4361873/150‑1 (MQ=255)

                                                                     atGCGAAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGCTAGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGCATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCa                                                                             >  1:3770626/1‑150 (MQ=25)

                                                                         ggAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGCGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAg                                                                         >  1:1954213/1‑150 (MQ=255)

                                                                             gtagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTa                                                                     <  2:4498186/150‑1 (MQ=255)

                                                                              tagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTATCGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAg                                                                    >  1:86313/1‑150 (MQ=255)

                                                                               agtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAgg                                                                   >  1:1728560/1‑150 (MQ=255)

                                                                                gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAATGGATAGAGCGTTTGGCTACGGATCAAAAGGTTAggg                                                                  >  1:2781514/1‑150 (MQ=255)

                                                                                 ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAgggg                                                                 <  1:1800765/150‑1 (MQ=255)

                                                                                  tCAGTGGTAGAACACCACCTTGCCAGGGTGGGGGTCCCGGGTTCGAATCCCGTCTCCCGCTCCATTTATCCTAACGGGTTATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGt                                                                <  2:2542056/150‑1 (MQ=37)

                                                                                  tCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGGTAGGGGt                                                                >  1:395607/1‑150 (MQ=255)

                                                                                    aGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAACCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTc                                                              <  2:2131747/150‑1 (MQ=255)

                                                                                     gTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCg                                                             <  1:3315994/150‑1 (MQ=255)

                                                                                      tGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTCCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                                            <  2:78234/150‑1 (MQ=255)

                                                                                        gTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGAct                                                          <  2:3032600/150‑1 (MQ=255)

                                                                                        gTAGAACACCACCTTGCCAAGGGGGGGGTCGCGGGTTCGAATCCCGCCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGAct                                                          <  1:4370986/150‑1 (MQ=255)

                                                                                             acacCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCctct                                                     >  1:1083849/1‑150 (MQ=255)

                                                                                             acacCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGACATTGTAAATTGAATTGCCCCCGTAGCTCAATTGGAAAGAGCGTTTGACTACGGATCATAAGGATAGGGGTTCGACTCctct                                                     >  1:4493962/1‑150 (MQ=37)

                                                                                              ccccaccTTGCCAGGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCtctc                                                    <  2:3005145/148‑1 (MQ=255)

                                                                                              ccccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCtctc                                                    <  1:4008389/148‑1 (MQ=255)

                                                                                              caccacCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGGTCGACTCCtctc                                                    >  1:2661025/1‑150 (MQ=255)

                                                                                                ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGGCTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCgg                                                  >  2:4133477/1‑150 (MQ=255)

                                                                                                  accTTGCCAAGGTGGGGGTCGCGGGTCCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgc                                                <  1:4861941/150‑1 (MQ=255)

                                                                                                  accTTGCCAAGGTGGGGGCCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgc                                                <  1:1392123/150‑1 (MQ=255)

                                                                                                   ccTTGCCAAGGTGGGGGTCGCGGGTTCGACTCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgcg                                               <  1:4800351/150‑1 (MQ=255)

                                                                                                     ttGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgcgcg                                             >  1:4610198/1‑150 (MQ=255)

                                                                                                         cAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCat                                         <  2:4536449/150‑1 (MQ=255)

                                                                                                         cAAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCat                                         <  1:3008425/150‑1 (MQ=255)

                                                                                                          aaGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGGTAGGGGTTCGACTCCTCTCGGGCGCGCCata                                        >  2:235219/1‑150 (MQ=255)

                                                                                                            ggTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGGTAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATc                                      >  1:2288644/1‑150 (MQ=255)

                                                                                                             gTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCt                                     >  1:3924666/1‑150 (MQ=255)

                                                                                                              tGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCtt                                    >  2:915267/1‑150 (MQ=255)

                                                                                                                ggggTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCtttt                                  <  1:2488056/150‑1 (MQ=255)

                                                                                                                   gTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGGGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaat                               >  1:2238954/1‑150 (MQ=255)

                                                                                                                    ccgcgGGTTCGAATCCCGCCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaata                              <  1:1382061/149‑1 (MQ=255)

                                                                                                                      gcgcGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGCATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaataga                            >  1:2604236/1‑150 (MQ=255)

                                                                                                                        gggTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAGATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaat                          <  1:2698105/150‑1 (MQ=255)

                                                                                                                         ggTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaata                         >  1:4681983/1‑150 (MQ=255)

                                                                                                                            tCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAatagat                      >  2:2624243/1‑150 (MQ=255)

                                                                                                                              gactCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAatagatag                    <  1:804778/147‑1 (MQ=255)

                                                                                                                              gAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAatagatag                    >  2:1124478/1‑150 (MQ=255)

                                                                                                                                aTCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGa                  <  1:3819/150‑1 (MQ=255)

                                                                                                                                 tCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGaa                 >  1:4396739/1‑150 (MQ=255)

                                                                                                                                  cccGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTCGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGaaa                >  1:1354406/1‑150 (MQ=255)

                                                                                                                                   ccGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAAt               <  2:4105730/150‑1 (MQ=255)

                                                                                                                                    cGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATc              >  1:2405791/1‑150 (MQ=255)

                                                                                                                                      tCTTCCGCCCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCgg            <  1:3352510/150‑1 (MQ=255)

                                                                                                                                         tCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGaa         <  2:1728560/150‑1 (MQ=255)

                                                                                                                                         tCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGaa         >  1:2165215/1‑150 (MQ=255)

                                                                                                                                            gCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATGGGAAATCGGGAAGTa      <  2:4821617/150‑1 (MQ=255)

                                                                                                                                            gCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTa      <  2:1276608/150‑1 (MQ=255)

                                                                                                                                               ccATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCt   >  1:2086936/1‑150 (MQ=255)

                                                                                                                                               ccATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCt   <  2:1202732/150‑1 (MQ=255)

                                                                                                                                               ccATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCt   <  2:4744688/150‑1 (MQ=255)

                                                                                                                                               ccATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCt   <  1:641867/150‑1 (MQ=255)

GCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGCTC  >  BS168_reference_genome/165888‑166180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`