| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 3,935,823 | Δ1 bp | 100% | intergenic (+35/+1) | ywbE → / ← rlmI | conserved protein of unknown function/large subunit ribosomal RNA m5C1962 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 3,935,823 | 0 | T | . | 100.0% | 123.4 / NA | 28 | intergenic (+35/+1) | ywbE/rlmI | conserved protein of unknown function/large subunit ribosomal RNA m5C1962 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (15/13); total (15/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCATTTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCA > BS168_reference_genome/3935705‑3935953 | aaaTCAAACTTCAAACCGCATGGCATTAAAGTCCGTCTTGAAGTTGGCCGCAGCGCCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGAc < 5:57048/139‑1 (MQ=255) tCATCCGCATGGCATTAAAGTCCGTCTTGAAGAAGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTATTTCTGCAGAAGGACCACTTTCAgg > 5:107725/1‑138 (MQ=255) cGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCg > 1:123781/1‑138 (MQ=255) cGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCGTTTTCA‑TTATTTTTTTTGCAGAAGGACCACTTTGAGGTAGTTGCCTTGCGGAAATGGCg > 3:26829/1‑138 (MQ=255) cGTCTTGAAGATGGCCGCATCGGCCGCGACCACCAGCTCGATACTACAT‑AAAAAAAAGCAGCCCCGGAACAGGGCAGCGTTTTCA‑TTAATTTTAATGCAGAAGGCACCCTTTCAGGTAGTATCCTTCAGGAAATgacc > 6:168717/1‑135 (MQ=255) ggCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGa > 1:117259/1‑138 (MQ=255) gCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGGTTTGaaa > 1:143803/1‑139 (MQ=255) ccccGCGACCAGAATATCGTTCAAACAA‑AAAAAAACGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCgg < 2:143803/137‑1 (MQ=255) ccGCGACCCGAATTTCGTTTCTAAAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCa < 2:117259/139‑1 (MQ=255) gACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCg > 4:204653/1‑138 (MQ=255) agcagATCGTTTCTACATAAAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTaaa < 3:131555/139‑1 (MQ=255) cagATCTTTTCTAAAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTc < 7:251845/139‑1 (MQ=255) cagATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAAt < 2:123781/137‑1 (MQ=255) gTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCaa < 2:127342/138‑1 (MQ=255) ttCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGa > 1:7931/1‑139 (MQ=255) cAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCgtg < 5:204772/139‑1 (MQ=255) aaaaaaaaGCTGCCCCTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAg > 5:76608/1‑139 (MQ=255) ccccTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTACGGCAGCGTAAATTCCTCAATGATCGTGTAGCTTTAAttttt < 3:204653/138‑3 (MQ=255) ccccTGAACAGGGCAGCTTTTTCA‑TTATTTTTTCAGCAGAAGGACCACTTTCAGGTAGTTTCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTAAATGATCTTGTAGCGTTGATTTGtt > 8:31019/1‑139 (MQ=255) gggCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGa < 7:289995/79‑1 (MQ=255) gggCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGa > 8:289995/1‑79 (MQ=255) ggCATCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCCTAAATTACTCAATGATCGTGTAGCGTTCATTTGTTTATTTAAAg > 2:4804/1‑137 (MQ=255) ggCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAACTCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggc > 2:243551/1‑139 (MQ=255) ggCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAg > 6:262381/1‑137 (MQ=255) ggCAGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGGAGCGTTCATTTGTTTCTTTAAAggc > 5:292762/1‑139 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggcggc < 7:127931/139‑1 (MQ=255) aGCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAggcggc < 7:31019/139‑1 (MQ=255) gCTTTTTCA‑TTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCa < 5:262381/139‑1 (MQ=255) | AAATCAAACTTTCATCCGCATGGCATTAAAGTCCGTCTTGAAGATGGCCGCATCGGCCGCGACCAGCAGATCGTTTCTACAT‑AAAAAAAAGCTGCCCCTGAACAGGGCAGCTTTTTCATTTATTTTTTCTGCAGAAGGACCACTTTCAGGTAGTTGCCTTCCGGAAATGCCGAAATCGTTTTGAAATCCTCCGGCAGCGTAAATTCCTCAATGATCGTGTAGCGTTCATTTGTTTCTTTAAAGGCGGCA > BS168_reference_genome/3935705‑3935953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |