| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,387,878:1 | +A | 100% | coding (711/735 nt) | yphF ← | putative lipoprotein of unknown function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,387,878 | 1 | . | A | 96.4% | 87.1 / ‑3.3 | 28 | coding (711/735 nt) | yphF | putative lipoprotein of unknown function |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (14/13); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑AAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAA > BS168_reference_genome/2387746‑2388010 | gtgtATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aa > 6:274606/1‑138 (MQ=255)gtgtATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aa > 1:66296/1‑138 (MQ=255) tATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aaaaa > 2:184654/1‑137 (MQ=255) tATGACGTTGTCCTATTTGAATATGACATATTTAGAAAATTTCTCATCCTAAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aaaaac < 8:56435/139‑3 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aaaaacag > 3:44463/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aaaaacag > 3:219007/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aaaaacag > 2:79050/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aaaaacag > 1:256458/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aaaaaaag > 4:67228/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGAAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aaaaacag > 4:131148/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATACTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑aaa‑aaaaacag > 7:116529/1‑135 (MQ=255) tGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATACTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑AAATAAAACAg > 8:16657/1‑139 (MQ=255) tGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCt > 2:127101/1‑139 (MQ=255) tCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGt > 8:273747/1‑139 (MQ=255) tCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGt > 8:164096/1‑139 (MQ=255) atatGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTa < 1:79050/139‑1 (MQ=255) atatGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTa > 3:133636/1‑139 (MQ=255) tGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTAcc < 1:127101/138‑1 (MQ=255) tGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTAcc < 7:273747/138‑1 (MQ=255) aCATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGta > 1:90461/1‑139 (MQ=255) tttCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCAtt < 3:205906/118‑1 (MQ=255) tttCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTACATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCAtt > 4:205906/1‑118 (MQ=255) aTCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgcg < 2:90461/137‑1 (MQ=255) aTCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgcg < 2:158269/137‑1 (MQ=255) aTCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAAcgc < 2:256458/136‑1 (MQ=255) aTCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGTGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTGATTTACCGTATATTTTACAGAAAACGCGGGGACaaa < 8:116529/138‑1 (MQ=255) atatAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGttt < 4:44463/138‑1 (MQ=255) aaaaaaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGc > 5:45624/1‑138 (MQ=255) aaaaaaCCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGc > 1:78514/1‑138 (MQ=255) aTTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGTTTGCATGCCAAATCATATCCTGGAtt > 4:82527/1‑139 (MQ=255) tttGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGAttt < 2:66296/139‑1 (MQ=255) aGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCggg > 7:3689/1‑139 (MQ=255) agaAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGttt > 7:29159/1‑138 (MQ=255) agaAAGGTTCATTCCTTTTTCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGttt > 1:158220/1‑138 (MQ=255) ttttCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGTGGACAAAGTGGTTTTCATGCCAAATCATATCATGGATTTCAGTCCCGGGTTTAAACGttttttttattt > 6:155806/1‑134 (MQ=255) ttttCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGcc > 2:115762/1‑91 (MQ=255) ttttCATATCATTTTCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGcc < 1:115762/91‑1 (MQ=255) tatCATTTTCTAAAA‑AAAACAGGTTCGTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGa < 7:16657/139‑1 (MQ=255) ttttCTAAAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCaa < 8:3689/139‑1 (MQ=255) | GTGTATGACGTTGTCCTATTTGAATATGACATCTTTAGAAAATTTCTCATCCTCAATCTATTAATATAAAAAAACCTTTCTCCCTATTTGTATGCAAGGGAGAAAGGTTCATTCCTTTTTCATATCATTTTCT‑AAA‑AAAACAGGTTCCTGTTTGTCATTTACCGTATATTTTACAGAAAACGCGGGGACAAAGGGGGTTTCATGCCAAATCATATCCTGGATTTCAGTCCCGGGTTTAAACGTTTTTTTGCTTTTTTTGAGGCAA > BS168_reference_genome/2387746‑2388010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |