| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 1,910,754 | C→T | 19.9% | S196S (TCC→TCT) | yndF → | putative spore germination lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,910,754 | 0 | C | T | 19.9% | 43.6 / 3.1 | 20 | S196S (TCC→TCT) | yndF | putative spore germination lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (9/7); new base T (2/2); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAATCAGGCTGAGCTGTCTTGTTTTATCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGCAGCAGCTTTTTACTGCAAAATGTGGTTGCGGCGAATGGAGCGGTCAAGTATTCCGGAGCAGCTGTAATCAACGGAAAATCG > BS168_reference_genome/1910627‑1910889 | aaaTCAGGCTGAGCTGTCTTGTTTTATCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGATTTCTTcc < 1:287016/139‑1 (MQ=255)aaaTCAGGCTGAGCTGTCTTGTTTTATCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAAttcttc < 3:33543/138‑1 (MQ=255) aGGCTGAGCTGTCTTGTTTTATCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTa < 5:293849/139‑1 (MQ=255) tCTTGTTTTATCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTcgc < 2:21890/138‑1 (MQ=255) tCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTTCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCTAGAATTCTTcc < 1:286845/113‑1 (MQ=255) tCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTTCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCTAGAATTCTTcc > 2:290441/1‑113 (MQ=255) tCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTTCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCTAGAATTCTTcc > 2:286845/1‑113 (MQ=255) tCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTTCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCTAGAATTCTTcc < 1:290441/113‑1 (MQ=255) tCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTg > 8:83674/1‑139 (MQ=255) aGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCt > 6:113911/1‑139 (MQ=255) agaCGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAAttctt < 3:271017/91‑1 (MQ=255) agaCGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAAttctt > 4:271017/1‑91 (MQ=255) aCGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGcagcag > 6:240593/1‑139 (MQ=255) ctctCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGCAGCAGCtt > 2:120717/1‑139 (MQ=255) gaACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGCAGCAGCTTTTTACTGCAAAAtgtg > 4:18721/1‑139 (MQ=255) cGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGCAGCAGCTTTTTACTGCAAAATGTGGtt > 4:202813/1‑139 (MQ=255) gCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGCAGCAGCTTTTTACTGCAAAATGTGGTTGCGGCGAATGGAg < 7:83674/139‑1 (MQ=255) ttCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGCAGCAGCTTTTTACTGCAAAATGTGGTTGCGGCGAATGGAGCGGt > 1:26517/1‑139 (MQ=255) ttAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGCAGCAGCTTTTTACTGCAAAATGTGGTTGCGGCGAATGGAGCGGTCAAGTATTCCGGag < 3:202813/139‑1 (MQ=255) tCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGCAGCAGCTTTTTACTGCAAAATGTGGTTGCGGCGAATGGAGCGGTCAAGTATTCCGGAGCAGCTGTAATCAACGGAAAATCg > 5:255872/1‑138 (MQ=255) | AAATCAGGCTGAGCTGTCTTGTTTTATCTGCTAAAGGGGAAGCAAGAGACGCTCTCCAGCTGAAAGAGAACGGAGAAATTCCGGCATTTCGGCTGATTGGATTAGGTGAAAATGAACATAAAGTTTCCAGAATTCTTCCCCCTATGACACTCGCGAAACTAATTGGAAAACTGCATTCTGGCAGCAGCTTTTTACTGCAAAATGTGGTTGCGGCGAATGGAGCGGTCAAGTATTCCGGAGCAGCTGTAATCAACGGAAAATCG > BS168_reference_genome/1910627‑1910889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |