| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,271,424 | T→C | 100% | R78R (AGA→AGG) | uvrX ← | lesion bypass phage DNA polymerase; phage SPbeta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,271,424 | 0 | T | C | 100.0% | 98.7 / ‑6.6 | 33 | R78R (AGA→AGG) | uvrX | lesion bypass phage DNA polymerase; phage SPbeta |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (12/20); minor base A (1/0); total (13/20) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAA > BS168_reference_genome/2271292‑2271558 | tCCTGCATCTATAAAAGATTCATCAATTGAATACGTATGGACACTTTTCTCAGGAAGAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGTATTTACAATGTGTATCCTTgg < 1:50041/138‑1 (MQ=14)tCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGa > 6:200380/1‑139 (MQ=255) aaTTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGtct < 4:294672/138‑1 (MQ=255) gAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGAt < 7:123814/138‑1 (MQ=255) aaaacaTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGATTCATTTGTGGATTTACAATTTCTATACTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGAtt > 4:114749/4‑139 (MQ=11) aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa > 8:99955/1‑138 (MQ=37) aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa > 1:63679/1‑138 (MQ=37) aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa > 6:209233/1‑138 (MQ=37) tttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaaaa < 8:8978/139‑1 (MQ=37) ttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAg < 4:297110/100‑1 (MQ=255) ttCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAg > 3:297110/1‑100 (MQ=255) aGGAACAAATCTGTAAAACAGCTATGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTATGATTCCAACATCtttt > 2:154133/1‑139 (MQ=255) aaTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTg > 8:52338/1‑139 (MQ=21) aaTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTg > 2:169607/1‑139 (MQ=21) aTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTg > 2:113296/1‑138 (MQ=21) aaaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt < 5:34520/139‑1 (MQ=21) aaaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCGTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGAGCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt < 6:139473/139‑1 (MQ=255) aaaCAGCGTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTCTCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt < 2:248442/139‑1 (MQ=255) aaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATg < 5:237011/139‑1 (MQ=21) gCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc < 3:105938/139‑1 (MQ=21) cTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTg > 8:231002/1‑65 (MQ=255) cTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTg < 7:231002/65‑1 (MQ=255) cTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc > 6:65095/1‑138 (MQ=21) aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa < 3:200763/138‑1 (MQ=12) aTTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTacaa < 5:209233/139‑1 (MQ=12) gtgGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAg < 5:65095/138‑1 (MQ=255) gATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa < 2:267178/122‑1 (MQ=12) gATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa > 1:267178/1‑122 (MQ=12) cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc < 1:288890/138‑1 (MQ=255) cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc < 5:200380/138‑1 (MQ=255) cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc < 7:52338/138‑1 (MQ=255) cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc < 1:169607/138‑1 (MQ=255) tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa < 1:113296/138‑1 (MQ=255) | TCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAA > BS168_reference_genome/2271292‑2271558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |