| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,633,093 | C→T | 100% | G43E (GGG→GAG) | yqxA ← | conserved protein of unknown function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,633,093 | 0 | C | T | 100.0% | 68.1 / NA | 23 | G43E (GGG→GAG) | yqxA | conserved protein of unknown function |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (4/19); total (4/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTTTGAAAACATATTGAATGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCCCCTTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCCGTGGTTTGCCTGCTGCATGCCGAACAGAACGCCTAAAAACATGATCGTGACAAGCAGTAAGCACTTTCCAA > BS168_reference_genome/2632959‑2633207 | cTTTTGAAAACATATTGAATGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCtt < 6:167660/139‑1 (MQ=255)cTTTTGAAAACATATTGAATGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGGCGGTGAGCGTAAACGCCTCCtt < 6:69291/139‑1 (MQ=255) aaaaCATATTGAATGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTg < 5:239615/138‑1 (MQ=255) aaaaCATATTGAATGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGa < 2:218082/139‑1 (MQ=255) aaaCATATTGAATGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGGGAGCGTAAACGCCTCCTTCAGTGa < 3:50304/138‑1 (MQ=255) gAATGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTGATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGa > 8:91866/1‑139 (MQ=255) aaTGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGa < 1:269037/138‑1 (MQ=255) aTGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATa < 2:233755/139‑1 (MQ=255) aTGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATa < 2:208772/139‑1 (MQ=255) aTGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATa < 8:274769/139‑1 (MQ=255) aTGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATa < 1:101900/139‑1 (MQ=255) tcCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGt < 7:9115/127‑1 (MQ=255) tcCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGt > 8:9115/1‑127 (MQ=255) cACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATAGCCtt < 6:295243/137‑1 (MQ=255) ttCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATAATCTTTTCCGTCGGTGAGCGTAAACGCCTCCATCAGTGAAGGGTAATGATAGGCTTTCATACt > 4:255570/1‑138 (MQ=255) ataAATCTTTTGCCGTGACTGTTTCTCGTAGGATTGAGGGTTCTTTTTCATTATCTTTTCCGTTGTTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCCGTGGTTTGCctgat < 4:8636/137‑3 (MQ=255) gTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGGGAGCGTAAACGCCTCCGTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCCGTGGTTTGCCTGCTGCATGCCGAACAGAACGCCTa < 6:210224/139‑1 (MQ=255) tttCTCCTAGGATGGAGGCTTCTTTTTCATTATCTTTTCCGTCGGGGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCCGTGGTTTGCCTGCTGCATGCCGAACAGAACGCCt < 5:96158/137‑1 (MQ=255) gATTGAGGCTGCTTTTTCATTATCTTTTCCTTCGGGGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCCGTGGTTTGCCTGCTGCATGCCGAACAGAACGCCTAAAAACATGATc < 6:237885/139‑1 (MQ=255) aGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCCGTGGTTTGCCTGCTGCATGCCGAACAGAACGCCTAAAAACATGATCGTGAc < 6:17285/139‑1 (MQ=255) cATTATCTTTTCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCCGTGGTTTGCCTGCTGCATGCCGAACAGAACGCCTAAAAACATGATCGTGACAAGCAGTAAGCa > 6:115448/1‑139 (MQ=255) ttgcGTCGGGGAGCGGAAACGCCTCCTTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCAGTGGTTTGCCTGCTGCATGCCGAACAGAACGCCTAAAAACATGATCGTGACAAGCAGTAAGCACTTTCCaa < 3:271376/135‑1 (MQ=255) ttCCGTCGGTGAGCGTAAACGCCTCCTTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCCGTGGTTTGCCTGCTGCATGCCGAACAGAACGCCTAAAAACATGATCGTGACAAGCAGTAAGCACTTTCCaa < 5:115448/138‑1 (MQ=255) | CTTTTGAAAACATATTGAATGTCTCCACCTTTTCCAGCTCTTTTTGTTTTTCTACTAAATCTTTTGCCGTGACTGTTTCTCCTAGGATTGAGGCTTCTTTTTCATTATCTTTTCCGTCGGTGAGCGTAAACGCCCCCTTCAGTGAAGGGTCATGATAGCCTTTCATACTCAGCATCCCGTGGTTTGCCTGCTGCATGCCGAACAGAACGCCTAAAAACATGATCGTGACAAGCAGTAAGCACTTTCCAA > BS168_reference_genome/2632959‑2633207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |