BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	121	G→C	5.4%	intergenic (–/‑2857)	– / → eryR	–//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	121	0	G	C	5.4%	54583.1 / inf	19276	intergenic (–/‑2857)	–/eryR	–//vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (8214/9097); new base C (12/1359); total (8246/11030)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 19597 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  Exported/1‑259

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  2:49854‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  5:222195‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  3:1797‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  1:33047‑M1/123‑1 (MQ=255)

ataaatgcttcaataaTCCAACCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTCCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          <  8:28236‑M1/123‑1 (MQ=255)

     tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                                      <  2:99807‑M1/127‑1 (MQ=255)

     tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGa                                                                                                                                      <  7:162267‑M1/127‑1 (MQ=255)

      gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                                    <  5:180072‑M1/129‑1 (MQ=255)

       cttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAAt                                                                                                                                   <  4:209797‑M1/130‑1 (MQ=255)

        ttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAAt                                                                                                                                   <  4:183976‑M1/130‑1 (MQ=255)

          caataatCCATACTCCAAAGATGGAGAGTGCTTTAAATGACGCAAAAATTAATCTTTCTGGTGAACCTTATAAAATTTTCGTTGAATTAATAGAAACATAGAG‑GTAAAATTAGCAGAAACTTAATAGAAAGGAAATGa                                                                                                                                 >  8:180349/7‑138 (MQ=255)

          caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  7:292611‑M1/132‑1 (MQ=255)

          caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                 <  2:137651‑M1/132‑1 (MQ=255)

          caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGAAGTAACTTAATAGAACGTAAATGa                                                                                                                                 <  6:215244‑M1/132‑1 (MQ=255)

                tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                          >  2:288660/1‑139 (MQ=255)

                tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                          >  6:259753/1‑139 (MQ=255)

                tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGAAACTTAATAGAACGGAAATCAAAAAAGc                                                                                                                          <  1:165848/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATTAAAAAAGCCa                                                                                                                        <  8:141540/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  7:144815/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  5:81858/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  7:103217/139‑1 (MQ=255)

                  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        >  8:160505/1‑139 (MQ=255)

                  cATCCTCCAAAGTGGGAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCG‑GAAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                        <  1:227328/139‑1 (MQ=255)

                  cATCCTCAAAATTTGGAGATGTAGTTTTATGTCGCAAATATTAATGTTTCTGGTTAACCTTATAAAATTTTCGTTGATTTAAAAGAAACATAGCG‑GTAAAATTAGCAGTAACTAAATACACCGGAAATTAAAAAAGCCa                                                                                                                        <  8:81841/139‑1 (MQ=255)

                  cATCCTCAAAAGTGGGAGAGTGAGTTTTATGGCGCAAATATAAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTCGCAGTAACTTAATAGAACGGAAATGAAAAACGCCa                                                                                                                        <  3:297345/139‑1 (MQ=255)

                    tcctccAAAGTTGGAGTGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACATTATCAAATTTTCGTTGATTTAATAGAAACAACGGG‑GTAAAATTAGCAGTAAATTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      <  3:158288/139‑1 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAATAAAGCCAct                                                                                                                      >  3:72066/1‑139 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      >  6:60965/1‑139 (MQ=255)

                    tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                      <  1:90592/139‑1 (MQ=255)

                         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                          >  4:36361/1‑114 (MQ=255)

                                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                          >  1:248469/1‑139 (MQ=255)

                                 tagtgtgattTTTATGTCTAAAAATTTAATGTTTGTGGTGAAAATTAAAAAATTTTGTTTTATTAAATATAAACATTGCG‑TAAAAATTACCAGAAAATTAAAACACCGCAAATCAAAAACGCCACTCTCATATGCTAtt                                                                                                         <  4:15875/135‑1 (MQ=255)

                                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGACACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtc                                                                                                         >  7:66070/1‑138 (MQ=255)

                                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCa                                                                                                                        <  2:74259/124‑1 (MQ=255)

                                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         >  4:113499/1‑139 (MQ=255)

                                 gagaGTGAGTTTTATGTCACAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                         <  1:122894/139‑1 (MQ=255)

                                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGTCt                                                                                                     >  5:24309/1‑138 (MQ=255)

                                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                    >  7:249934/1‑139 (MQ=255)

                                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                    >  5:282979/1‑139 (MQ=255)

                                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                    >  3:220355/1‑139 (MQ=255)

                                        tgtTTTATGTCGCAAATATAAATGTTTCTGGGCAACCTTATAAAATTTTCGTTGATTTAATAGAAACATCGCG‑GTAAAATTAGCAGAAACTTAATAGAACGTAAATGAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  8:121955/138‑1 (MQ=255)

                                        cgtTTTATGTCGCAAATATTAATGTTTCTGGTGACCCTTATCAAATTTTCGTTGTTTTAAAAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  3:138620/138‑1 (MQ=255)

                                        atgtttATGTCGCAAATATTAATGTTACTGGTGAACCTTATAAAATTTTCGTTGATTTAATACAAAAATAGCG‑GAAAAATTAGCATAAACTAATTATAACGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  2:209402/136‑1 (MQ=255)

                                         tttttATGTCTAAAATATTTATTTTTGTGTTTAAACTTATAAAATTTTCTTTTATTTAATATAAACTTAGCG‑TTAAAATTAGCATTAAATTAATAGAACGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  2:187973/137‑1 (MQ=255)

                                         tttttATGTCGCAAATATTAATGTTTCTGTTTAACCTTATCAAATTTTCGTTTATTTAATATAAACATAGCT‑GTAAAATTAGCATTAACTTAATATAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                  <  3:118769/137‑1 (MQ=255)

                                              ttGTCCCAAATATTAATGTTTCTGTTGAACCTTATAAATTTTTCGTTTATTTAAAATAAAAAAAGCG‑TTAAAATTAGCAGTAAATTAATACACCGCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCt                                                                                             <  7:123845/137‑1 (MQ=255)

                                                 tccaaaaTATTATTGTTTCTGGTTAACATTATAAAATTTTCGTTTATTAAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAg                                                                                         <  8:40846/135‑1 (MQ=255)

                                                   gCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  3:200047/1‑138 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGACCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGAG‑GTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGACACTCTCATATGATATTGCCTACAAACCTTTAGc                                                                                        >  8:6193/1‑137 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTCCCAACCTTTAGc                                                                                        >  4:231351/1‑137 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCGACATGTAGc                                                                                        >  5:39725/1‑137 (MQ=255)

                                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                        >  1:100158/1‑137 (MQ=255)

                                                       ttatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAAAATAGCG‑GTAAAATTAGAAGTAACTTAATAGAACGGAATTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  7:274035/138‑1 (MQ=255)

                                                       ctatTACTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACAACGCG‑GTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  5:119893/138‑1 (MQ=255)

                                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  1:69898/139‑1 (MQ=255)

                                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  7:232115/139‑1 (MQ=255)

                                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACCCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  8:19995/139‑1 (MQ=255)

                                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTAAATACAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  5:158323/139‑1 (MQ=255)

                                                       atatTAATGTTTCGGGTGAACCTTATCAAATTTTCGTTTATTAAATAGAAACATCGCG‑GTAAAAATAGCAGTAACTTAATACAACGTAATTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                   <  4:278727/139‑1 (MQ=255)

                                                            aaTCTTTCTGGTGAACCTTATCAAATTTTCGTTGATTAAATACAAACATACCG‑GTAAAATTAGCAGTAACTTAATAGAACGCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  8:260201/139‑1 (MQ=255)

                                                             aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  3:278191/138‑1 (MQ=255)

                                                                tttCTGGTGAACATTATAAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                           <  3:258183/138‑1 (MQ=255)

                                                                 ttCAGGTGAACCAAATCAAATTTTCGTTCAAATAAAAGAAACAAAGCG‑CTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAAAGCCACACTCATATGCTATAGGCTACCAACCTTAAGCGAGAATGCCTTAATc                                                                         >  4:59538/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  4:254010/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  1:273552/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  1:144090/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                        >  5:102195/1‑139 (MQ=255)

                                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATca                                                                        >  6:166934/1‑138 (MQ=255)

                                                                  tCTGGTGAACATTATCAAATTTTATTTGATTTAATAGAAACATAGAG‑GTAAAATTAGCAGTAACTTAATAGAACTGAAATGAAAAAAGCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATTACTTAATcc                                                                        >  6:141396/1‑139 (MQ=255)

                                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACttctgtct                                                                       >  1:183829/1‑133 (MQ=255)

                                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                       >  4:80606/1‑139 (MQ=255)

                                                                     ggTGAAGCTTATAAAATTTTCGTTTATTAAAAACAAAAATAGCG‑GTAAAATTAGCATTAACTTAATAGACCCGAATTTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                      <  6:191675/138‑1 (MQ=255)

                                                                      gtcaaCCTTATAAAATTTTCGTTTATTTAATACAAACATAGCG‑TTAAATTAACCATTAACTTAATAGAACGCAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  2:232376/136‑1 (MQ=255)

                                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                                      <  2:165938/137‑1 (MQ=255)

                                                                      gTGAAACTAATAAAATTTACGTTTATTTAATAGAAACA‑ACCGTGAAAAATAACAAGTAACTAAATACAACGCAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                    <  6:38015/139‑1 (MQ=255)

                                                                       tGAACATTATCATATTTTGGTTGATTTAATAGAAAAATTGCG‑GTAAAATTAGCATTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTTCCTACCTTTAGCGAGAATGACTTAATCCTGTAc                                                                   <  2:26012/139‑1 (MQ=255)

                                                                           ccTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGc                                                                >  7:198016/1‑138 (MQ=255)

                                                                              tATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              >  8:219155/1‑121 (MQ=255)

                                                                              tATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCAt                                                             >  7:83451/1‑138 (MQ=255)

                                                                              tATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                            >  8:23892/1‑139 (MQ=255)

                                                                               aTCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTGGCGAGAATGACTTaa                                                                           <  6:121492/123‑1 (MQ=255)

                                                                                     ttttCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCa                                                                                                                        <  1:51612/72‑1 (MQ=255)

                                                                                       ttCTTTTATTTAATACAAACATAGCG‑GAAAAATACGCCTAAAATTAATATACCGGAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    <  2:110655/138‑1 (MQ=255)

                                                                                       ttCGTTCATTTAATATAAAAATAGCG‑GTAAAATTAGCAGTAACTTAATAGACCGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                    <  7:179021/138‑1 (MQ=255)

                                                                                         cGTTTATTAAAAAGAAAAATCGCG‑TAAAAATTAGCATTAACTAAAAACACCGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGa                                                 <  1:204719/139‑1 (MQ=255)

                                                                                                        aaCATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                              <  5:13610/95‑1 (MQ=255)

                                                                                                          cATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCaa                                >  5:264523/1‑139 (MQ=255)

                                                                                                           atcgCG‑GTAAAATTAGCAGTAACTTACTAGAACGCAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAAc                               <  6:84254/136‑1 (MQ=255)

                                                                                                            tAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                    >  5:204007/1‑69 (MQ=255)

                                                                                                            tAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACt                              >  4:136754/1‑139 (MQ=255)

                                                                                                             aGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                             >  3:91280/1‑139 (MQ=255)

                                                                                                               cG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCa                           <  7:47088/139‑1 (MQ=255)

                                                                                                                    aaatatAGCAGATACTTAATTGATCTTAATGGAAATACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGTATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATa                       <  3:47991/134‑1 (MQ=255)

                                                                                                                           gCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAtt                >  7:216227/1‑139 (MQ=255)

                                                                                                                                aaCTTAATAGAACCGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc            <  6:28149/138‑1 (MQ=255)

                                                                                                                                 aCTTATTAGAACGGATTTGAAAAAAGCCACTCTCATATGGTATTGGCTACCAAGCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGTCGTATATGAGGCGCGAACTTCAATGAAGTTATTTGCGtt          >  6:236720/1‑139 (MQ=255)

                                                                                                                                 aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt          >  8:100662/1‑139 (MQ=255)

                                                                                                                                     aaTAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGttt      >  5:61799/1‑139 (MQ=255)

                                                                                                                                       tAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTc    <  3:239122/139‑1 (MQ=255)

                                                                                                                                         gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc  >  6:101154/1‑139 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCG‑GTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGC  >  Exported/1‑259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`