BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,214	C→T	11.0%	intergenic (–/‑764)	– / → eryR	–//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,214	0	C	T	11.0%	132.2 / 4.8	46	intergenic (–/‑764)	–/eryR	–//vntifkey=4
Reads supporting (aligned to +/- strand): ref base C (17/23); new base T (2/3); total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATG  >  Exported/2098‑2344

tCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCtttatttta                                                                                                              <  5:284126/139‑1 (MQ=255)

 cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTATCCCTCATCTCGTCGCTATATATCtttatttta                                                                                                              >  1:140554/1‑138 (MQ=255)

 cGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttatttaat                                                                                                             >  3:96221/1‑138 (MQ=255)

   ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCa                                                                                                           >  4:69883/1‑139 (MQ=255)

          agtcaagttatatgtatctataatgatttgtggaagttttgccacaacagttggtttatttacaatcttttttttattagccgtcaaatttctccctcatcttgTCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                     <  5:78085‑M2/35‑1 (MQ=255)

                    gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATaa                                                                                                        <  8:48284/125‑1 (MQ=255)

                    gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATaa                                                                                                        >  7:48284/1‑125 (MQ=255)

                          tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACATTC‑TTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgc                                                                                    >  4:287643/1‑139 (MQ=255)

                          tGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                   <  3:256517/139‑1 (MQ=255)

                            tATCTATAATGATTTGTGGAAGCTTAGCCACAACAGGTGGTTTATTTACAATC‑TTTTTTTTCTTAGCCGGCAAATTTCTCCCTCCTCTCGTCTCTTTATATCTTTCTTTTATCATAAAGGAATATTTGAACCGTcgcg                                                                                   <  7:144383/138‑1 (MQ=255)

                              tCTATAATGCTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTT‑TTTGCCGTCAAATTTCTCCCTGTTGTAGTCTCTTTAGTTCATTTTTTTAGCATATAGGCGTTTTTGAACCGTCGCGCggg                                                                               >  2:229838/1‑139 (MQ=255)

                              tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCGAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                     <  4:54448/118‑1 (MQ=255)

                              tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCGAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                     >  3:54448/1‑118 (MQ=255)

                              tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                                                <  4:191880/107‑1 (MQ=255)

                              tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttattt                                                                                                                >  3:191880/1‑107 (MQ=255)

                              tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  7:189293/1‑139 (MQ=255)

                               cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              >  4:149893/1‑139 (MQ=255)

                                  ttaTGATTTGTGGTAGTTTTTCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCTTATAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             <  2:140554/136‑1 (MQ=255)

                                  taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             <  5:165308/138‑1 (MQ=255)

                                  taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTTTATATTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              >  7:188421/1‑137 (MQ=255)

                                  taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             <  4:245940/138‑1 (MQ=255)

                                  taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCACCCTCAACTTGACACTTTATATCATTATTTTATAATAAAGTATTATTTGAACCGTAGCGCGGGa                                                                              >  6:196918/1‑137 (MQ=255)

                                  taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  3:148831/136‑1 (MQ=255)

                                  taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  6:229101/136‑1 (MQ=255)

                                  taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             <  3:149893/137‑1 (MQ=255)

                                       tgtggaagttttgccacaacagttggtttatttacaatctttttttattagccgtcaaatttctccctcatctcgTCTCGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                                                                    <  2:187288‑M2/36‑1 (MQ=255)

                                       tgtggaagttttgccacaacagttggtttatttacaatctttttttattagccgtcaaatttctccctcatctcgTCTCGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                                                                    >  1:187288‑M2/76‑111 (MQ=255)

                                       aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGtt                                                                        <  1:25688/138‑1 (MQ=255)

                                        tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttatttta                                                                                                              >  6:288646/1‑98 (MQ=255)

                                        tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttatttta                                                                                                              <  5:288646/98‑1 (MQ=255)

                                          tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTACATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATg                                                                    >  5:160314/1‑139 (MQ=255)

                                            tgGAGGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTATATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGAt                                                                  <  8:234033/139‑1 (MQ=255)

                                            tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              >  1:175428/1‑127 (MQ=255)

                                            tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  2:175428/127‑1 (MQ=255)

                                            tgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGCCAGGTTTATGa                                                                   >  7:174238/1‑138 (MQ=255)

                                            tgGAAGTTATGCCACAACAGTTGGATTATTTACAATC‑TTATTTTTATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGa                                                                   <  4:149236/138‑1 (MQ=255)

                                                      gCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCTCTCAGCTCGTCTCTTTATATCTTTATTTTATGATAAAGGAGTATTTGAACCGTGGCGCGGGACAGGTTTATGATAGGGATAttt                                                        >  6:152371/1‑139 (MQ=255)

                                                                   gTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTAATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  4:96221/139‑1 (MQ=255)

                                                                    tttatttaCCATC‑TTTTTTTTATTAGCCTTCAACTTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTCTCATACAGGAGTAGTTGAACCGGCGCTCGGGACAGGTTTATTATAGGGATATGTTATTGAATAATTGa                                          <  5:288320/139‑1 (MQ=255)

                                                                    tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  3:69883/139‑1 (MQ=255)

                                                                     ttatttaCAATC‑TTTTTTTTATTAGCCGTCATATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  5:196918/138‑1 (MQ=255)

                                                                      tatttaCAATCTTTTTTTTTATTAGCCGTCATATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  2:91625/138‑1 (MQ=255)

                                                                              aTC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATTATATGGATATTTTTTTGAATAATTTATGGTATAAgg                                >  6:292533/1‑139 (MQ=255)

                                                                               aatctttttttATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             <  7:111085/90‑1 (MQ=255)

                                                                               aatctttttttATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             >  8:111085/5‑94 (MQ=255)

                                                                                                             ccTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATg  <  5:292533/139‑1 (MQ=255)

TCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATG  >  Exported/2098‑2344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`