BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,729	T→G	6.8%	intergenic (+14/‑142)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,729	0	T	G	6.8%	41021.5 / inf	13697	intergenic (+14/‑142)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (979/11466); new base G (1187/37); total (2169/11528)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 14363 total aligned reads displayed.

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  Exported/3591‑3872

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑ttt                                                                                                                                                >  5:282290/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑ttt                                                                                                                                                >  1:99204/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTATATGAGTCGC‑gtt                                                                                                                                                >  3:91979/1‑139 (MQ=255)

    tACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑tttt                                                                                                                                               <  2:156465/138‑1 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAATTAATTCTATGAGTCGC‑GTTTTTTAAtttt                                                                                                                                      >  8:205533/1‑138 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTTAtttt                                                                                                                                      >  4:256827/1‑138 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                      >  7:98779/1‑139 (MQ=255)

               aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGAATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                    <  3:294977/138‑1 (MQ=255)

                 tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAg                                                                                                                                 >  3:253786/1‑139 (MQ=255)

                     atcaAGCAATGAAAGACGCCAATGTAAACAATTTAAGTACCATTAATTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGATAGTTa                                                                                                                              <  5:106632/138‑1 (MQ=255)

                     atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac                                                                                                                             <  1:206652/139‑1 (MQ=255)

                     atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                              <  4:37186/138‑1 (MQ=255)

                      tcaAGCAATGCAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  3:212504/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  3:11100/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  8:165098/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  8:119928/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  1:227678/139‑1 (MQ=255)

                      tcaAGCAATGAAAAACGCCATAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  4:13586/139‑1 (MQ=255)

                      taaaGAAATTAAACAAGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATTATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  8:263944/137‑1 (MQ=255)

                      aaaaGCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                            <  2:202308/137‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                          <  7:157093/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                          <  7:247036/139‑1 (MQ=255)

                        aaGCAATGAAAAACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                          <  1:141678/139‑1 (MQ=255)

                             aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                     <  7:174831/139‑1 (MQ=255)

                              tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGTAATTTAAACCTTACt                                                                                                                     >  6:137100/1‑138 (MQ=255)

                              ataaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTa                                                                                                                    <  2:180310/137‑1 (MQ=255)

                                    acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                              <  5:1265/139‑1 (MQ=255)

                                      gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAATTTAAACGGTAATaaatctgtc                                                                                                            >  4:186399/1‑133 (MQ=255)

                                           aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                       <  4:115019/139‑1 (MQ=255)

                                            gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAATGGAATGGAGAt                                                                                                      <  3:274267/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                     <  5:3516/139‑1 (MQ=255)

                                             tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                     <  6:206798/139‑1 (MQ=255)

                                               aaCAATTTTAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaa                                                                                                    <  1:119536/138‑1 (MQ=255)

                                               aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                   <  4:86723/139‑1 (MQ=255)

                                               aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                   <  1:42561/139‑1 (MQ=255)

                                               aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                     <  1:286816/137‑1 (MQ=255)

                                                aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTTATTTTGGTATTTTCACGGTAAGAAAAGGAGAGGAGTTAAat                                                                                                  >  4:277723/1‑139 (MQ=255)

                                                aCAATTTAAGTACCATTACTTATGAGCAAGAATTGTCTATTTTAAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTT‑GAAAGATAAAACTATCTAAAGGGTATAGAGATtaaa                                                                                                  >  7:195006/1‑138 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  2:281827/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  5:95995/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  6:183716/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  5:55509/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  5:221261/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  1:100375/139‑1 (MQ=255)

                                                    tatAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                              <  6:58623/137‑1 (MQ=255)

                                                       aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                            <  1:62641/138‑1 (MQ=255)

                                                        aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                          <  5:160255/139‑1 (MQ=255)

                                                         gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAATGGAATGGAGATAAATTATTAGata                                                                                         <  7:40208/139‑1 (MQ=255)

                                                         gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                         <  3:189958/139‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                       <  1:268096/138‑1 (MQ=255)

                                                            ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTGTAAGTTTAACGTTTATAAATGGAATGGATATAAATTATTTtatataa                                                                                      >  2:88452/1‑138 (MQ=255)

                                                                  cTTATGAGCTAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTTTTAATTTA‑ACGGTTATAAATGGAATTGAGATTAATTATTATATataataatt                                                                                 >  6:11788/1‑135 (MQ=255)

                                                                  cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                <  6:257795/139‑1 (MQ=255)

                                                                  cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                <  8:225702/139‑1 (MQ=255)

                                                                   atATGAGGTAGTATTGTGTAGTATTATTAGTGATCTATTAGTTAAGGGGAGGATATTATTCAATGAGTCGC‑TGTTTTAAATTTGGAAAGTTACACGTTACTCAAGGGAATGGAGATATATTATTAGATTTTCTTCTGAc                                                                               <  4:232377/138‑1 (MQ=255)

                                                                    tATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTTATTTTGAAATTTTCACCTTTATTAATGGTATGGAGATAAATTATTATATATAGAACTGACa                                                                              >  6:280607/1‑139 (MQ=255)

                                                                       gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTTAAGTTTAACCTTTATAAAAGGGATGGTTGTAAATTTTTATTTATAAAAATGACAGca                                                                           >  6:80971/1‑138 (MQ=255)

                                                                       gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAACTTTCACGGTTAGAAAAGGTATTGTGTTAAATTATTTTATATTCTAATTACAGcg                                                                           >  7:266698/1‑138 (MQ=255)

                                                                        aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTGAAAGTTTAACGTTTATAAATGGAATGGTGATAAAATATTAGATTTAAAACTTACAgagt                                                                          >  1:82031/1‑136 (MQ=255)

                                                                           aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                       <  3:130172/139‑1 (MQ=255)

                                                                           aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                       <  2:253822/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                     <  8:284311/139‑1 (MQ=255)

                                                                             gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTACTAGATATACTACTGACAGCTTCCaag                                                                     <  1:185024/139‑1 (MQ=255)

                                                                                 tttCTTTTTTTTATATTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                  <  5:14296/136‑1 (MQ=255)

                                                                                        ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAAGTTTCAAGATAATAAAAGGGATGGTGATTAAATTTTTGATTTAAAAATGAAAGCATCCAAAAAACTaaaa                                                            >  8:184138/1‑136 (MQ=255)

                                                                                         tttAATATTTATATTTTATTTATCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAATGTTACACGTTAATAAAGGGAATGGAGATAAATTATTTGATATACTACTTAAAGATTCCAAGAAGCTAAtgag                                                          <  4:160508/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                          <  7:287328/138‑1 (MQ=255)

                                                                                         tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                          <  5:200939/138‑1 (MQ=255)

                                                                                             aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTccc                                                     <  2:126195/139‑1 (MQ=255)

                                                                                                gTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                  <  3:50993/139‑1 (MQ=255)

                                                                                                        ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTGGAAAGTTTAAAGTTTCTAAAGGGTATTGAGATAAATTATTAGATTTACAAATGACAGCTTCCAAGAAGCTAAATAGGTGCCTAGCGCCTACg                                          >  3:148928/1‑139 (MQ=255)

                                                                                                          attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAGAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                         <  7:61879/138‑1 (MQ=255)

                                                                                                           tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                        <  5:76354/138‑1 (MQ=255)

                                                                                                               aCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AAt                                   <  6:35666/138‑1 (MQ=255)

                                                                                                                   gaggaAATAATTCTATGAGTCGC‑GTTTTTAAATATGGAAAGTTACCCCGTACAAAAGTGGATGGTGATAAATTATTATATAAAAGACTGACACCGTACAAGCAGCGAAAAAGCTCCCAAGCGCCAACGGGG‑CATTTggg                              >  8:62449/1‑137 (MQ=255)

                                                                                                                    aggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGggg                             <  2:6460‑M1/139‑4 (MQ=255)

                                                                                                                    aggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGggg                             <  6:157123‑M1/139‑4 (MQ=255)

                                                                                                                    aggaAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAAATTAAACGTTAATAAAAGGGATTGATATTAAATTTTATATTTAAAAATTACATCTTACAAGAAGCTAAATAGGGGCCTAACGCCAAACGGG‑AAttttggg                             >  2:43467/1‑135 (MQ=255)

                                                                                                                     ggaAATAATTCTATGAGTCGC‑GTTTTTTTATTTTGTATGTTACACGTTACTAAAGGGAATGGGGATTAATTATTAGATATACTACTGACAGCTTCCAAGCAGCGAAAGCGGTACCTAGCGCCTAGGGGG‑AATTTggggt                            >  7:138896/1‑135 (MQ=255)

                                                                                                                     ggaAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGGAAGTTAAACGGTTCAAAAAGGAATTGAGATTAAATATTATATATAATAATGACACCTTACCACAACGAAAAGAGGTACCGAGCCCCGTCGGGGCAATTTgggt                             >  6:230184/1‑136 (MQ=255)

                                                                                                                             ttCTATTATGCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattga                     <  4:211047‑M1/138‑12 (MQ=255)

                                                                                                                               cTATGAGTCGC‑ATATTTAAATTTGGAAACTTACACGTTACTACAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaa                   <  8:82979‑M1/138‑14 (MQ=255)

                                                                                                                                  tGAGTCTC‑TTTTTTAAATTTGTAAAGTTACACTTTACTAAAGGTAATGTAGATAAATTATTAGATATACTACTGACAGCTTCAAAGAATCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTTgggtacattgaaaaagg               <  7:211952‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTATATTTGGATAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTTAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaagg               <  7:79870‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAATTTTGGAAAGTCACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTCCTGACAGCTTCCCAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaagg               <  2:229691‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaagg               <  7:229223‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaagg               <  1:164093‑M1/139‑18 (MQ=255)

                                                                                                                                  tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaagg               <  1:249182‑M1/139‑18 (MQ=255)

                                                                                                                                  tGACTCGC‑TTTTTTAAATTTGGAAATTTACACGTTACTAAAGGGAATTCACTAAAATTATTAGATATACATCTTACACCTTCCAACAAGCTAACGAGGTCCCTAGCGCATACGGTG‑AATTT‑‑‑‑‑GGGGTACATTGAAAAAgg          <  4:61770/139‑1 (MQ=255)

                                                                                                                                   gAGTCGG‑TTTTTTAATTTTGGAAAGTTACACGTTACTAAAGGGTTTGGAGATAAATTATTAGTTATACTACTGACACCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACTGGG‑AATTT‑‑‑‑‑GGGGTACATTGAAAAAgg          <  3:234925/138‑1 (MQ=255)

                                                                                                                                   gAGTCGA‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaagg               <  3:16793‑M1/138‑18 (MQ=255)

                                                                                                                                    attcgC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaaggaa             <  5:180495‑M1/137‑20 (MQ=255)

                                                                                                                                    aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAATAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaaggaa             <  8:243307‑M1/139‑20 (MQ=255)

                                                                                                                                    aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaaggaa             <  7:101528‑M1/139‑20 (MQ=255)

                                                                                                                                    aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaaggaa             <  7:121159‑M1/139‑20 (MQ=255)

                                                                                                                                    aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaagga              <  8:141420‑M1/138‑19 (MQ=255)

                                                                                                                                     gTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaaggaag            <  5:242434‑M1/139‑21 (MQ=255)

                                                                                                                                     gTCGA‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaaggaag            <  3:72502‑M1/139‑21 (MQ=255)

                                                                                                                                      tCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGACTGGAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaaggaag            <  8:37564‑M1/138‑21 (MQ=255)

                                                                                                                                        gC‑TTTTTTAAATTTGGAACGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGgggtacattgaaaaaggaagag          <  5:262549‑M1/138‑23 (MQ=255)

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGG‑AATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  Exported/3591‑3872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`