BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,741	G→T	9.8%	intergenic (+26/‑130)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,741	0	G	T	9.8%	41554.7 / inf	14337	intergenic (+26/‑130)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (393/12171); new base T (1472/220); total (1919/12418)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 14731 total aligned reads displayed.

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  Exported/3603‑3882

 tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTgg                                                                                                                                              <  5:275181/139‑1 (MQ=255)

 tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTgg                                                                                                                                              <  7:190788/139‑1 (MQ=255)

   aGTTTCATCATGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGa                                                                                                                                             <  3:285771/138‑1 (MQ=255)

     tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAAAGTGAGGAAATAATTCTATGAGTCGC‑GGTTTTATAATTTGTaaa                                                                                                                                           >  7:117410/1‑139 (MQ=255)

        catcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTa                                                                                                                                       <  2:275911/139‑1 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGAAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTa                                                                                                                                       <  5:121697/138‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                     <  8:281931/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                     <  6:248507/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                     <  2:119241/139‑1 (MQ=255)

          gacaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                     <  3:10715/136‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACg                                                                                                                                   <  5:256681/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACg                                                                                                                                   <  1:264165/139‑1 (MQ=255)

            aaGCAATGAAACAAGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACg                                                                                                                                   <  8:221816/139‑1 (MQ=255)

                 aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACt                                                                                                                              <  7:208994/139‑1 (MQ=255)

                  tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGAAATTTAAACCTTAca                                                                                                                              >  8:81789/1‑137 (MQ=255)

                    aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTaaa                                                                                                                           <  4:244375/139‑1 (MQ=255)

                         cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                      <  6:52536/139‑1 (MQ=255)

                           ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                                    <  3:6287/139‑1 (MQ=255)

                               aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGTAATTTAAACGTTAATAAAGGGAAtt                                                                                                                    >  6:75960/1‑134 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  6:108272/139‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  7:15400/138‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  3:205026/138‑1 (MQ=255)

                                     cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                          <  8:134447/139‑1 (MQ=255)

                                     cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                          <  5:196409/139‑1 (MQ=255)

                                      aaattAAGTACCATTACTTATGATCAATTATTGGCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAAATCTATGAGTCGC‑ATTTTTA‑AAATTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatta                                                                                                         <  8:36025/136‑1 (MQ=255)

                                        tttCAGTACCATTAATAATGAGCAAGTATTGTCTATTTTTAATAGTAATCTCATATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTT‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  7:263055/139‑1 (MQ=255)

                                        tttATGTTCCTTTTCTTATGAGCAAGTTTTGTCTATTTTTTCTAGTTATATATTATTTAACTGGATTAAATAATTCTTTGAGTAGC‑TTTTTTA‑AATTTGGAATGTTACTCTTTACTAAAGGGAATGGAGATTAattatt                                                                                                       <  4:17525/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  1:118955/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  3:41634/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  8:258704/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:69694/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  6:293129/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  2:222982/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  1:206464/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTAATTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  6:12887/139‑1 (MQ=255)

                                          tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTA‑AATTTGTAAAGTTTCAAGTTAAAAAAGGGTATGGAGATAAATTATTAg                                                                                                     >  2:61381/1‑139 (MQ=255)

                                           aactACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                     <  8:11225/135‑1 (MQ=255)

                                             gTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATATGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                  <  2:147979/139‑1 (MQ=255)

                                             gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                  <  3:162826/139‑1 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTT‑AATTTTGAAATTTAAACGTTTGTTAATGGTATTGTGATAAATTATtatatataa                                                                                               >  7:74221/1‑138 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                <  3:228028/138‑1 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                <  4:197168/138‑1 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATAtac                                                                                               <  3:245004/139‑1 (MQ=255)

                                                  aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGAAAGTTAAACGTTTATTAAAGGAATTGAGAAAAAATAATTtatataaa                                                                                              >  5:47771/1‑136 (MQ=255)

                                                    tACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGTAATTTAAACGTTTCTAAATGGAATTGAGATAAATTATTTTATAtactaat                                                                                           >  6:148894/1‑137 (MQ=255)

                                                         aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                        <  4:223858/137‑1 (MQ=255)

                                                         aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                        <  3:183406/137‑1 (MQ=255)

                                                           gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTT‑AATTTTGTAATTTTAACGTTACTAAATGGAATGGAGATAAATTATTTTATATACTACTGACAGcg                                                                                    >  8:157505/1‑138 (MQ=255)

                                                           gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGATAGTTTCACGGTTATAAAGGGGATGGGGTTTAATTATTAGATTTAGTAATTACAGcg                                                                                    >  8:60569/1‑138 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  3:82938/139‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTA‑ATTTTGGTATTTTAAAC‑TTAATAAAGGGAATGGAGATAAATTATTTGATATTATTATGACAGCTTGCaa                                                                               >  3:264308/1‑138 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTT‑AATTTTGAAAGTTTCACGTTTATAAAGGGTATGGAGATTAATTATTTTATATACTAATGACATCttac                                                                                 >  8:239203/1‑134 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  1:36519/139‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  2:84131/139‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTACTAGATATACTACTGACAGCTTCCaag                                                                              <  1:185024/139‑1 (MQ=255)

                                                                  tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  8:201832/139‑1 (MQ=255)

                                                                  tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  6:200366/139‑1 (MQ=255)

                                                                  tATTGTCTATTTTTAATAGATATCTATTATTTAACGGGAGGAAATAATTCTATGAGGAGC‑TTTTTTA‑AATTTGGAAAGATACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  2:248635/139‑1 (MQ=255)

                                                                    ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                           <  4:79525/139‑1 (MQ=255)

                                                                       tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                        <  1:281168/139‑1 (MQ=255)

                                                                         tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAACTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCACGAAGCTaaa                                                                      <  7:33748/139‑1 (MQ=255)

                                                                            ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGTAAGTTTAACGGTAATAAATGGAATGGAGATAAATTATTTTATATAATACTGACAGCTTGCAAGAAGCAAAagag                                                                   >  3:125850/1‑139 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                   <  4:52508/138‑1 (MQ=255)

                                                                                 aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTA‑AATTTGGAAAGTTTCACGTTACTAAAGGGTATGGAGATAAATTATTATATATACTAATGACATCGTCCAAGAATCAAAAGAGGTacc                                                              >  8:179016/1‑139 (MQ=255)

                                                                                  tAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCt                                                             <  5:213569/139‑1 (MQ=255)

                                                                                  tAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTA‑AATTTGGAAAGTTACCCGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCt                                                             <  6:221028/139‑1 (MQ=255)

                                                                                       aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTA‑AATTTTGAAACTTTCACGTTTATAAATGGTATTGAGATAAATTATTATATATACTACTGACAGCTTACAAGAAGCTAAAGAGGTCCCGAgcgc                                                        >  7:170407/1‑139 (MQ=255)

                                                                                              attTAACGGGAGGAAATAATTCTATGAGTCGA‑TTTTTTA‑AATTTGGAAAGTTACACGTTAATAAAGGGAATGGATATAAATTATTAGATATACTACTGACATCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                  <  2:197890/138‑1 (MQ=255)

                                                                                               tttAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGTAATTTAAACGTTTATAAAAGGTATGGAGATAAATTATTTTATATAATAAAGACATCATCCAATAAGCTAAAGAGGTCCCAAGCGCCAACgggg                                                >  3:143798/1‑139 (MQ=255)

                                                                                                  aaCGGGAGGAAATAATTGTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGaa                                              <  5:137681/138‑1 (MQ=255)

                                                                                                   aCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                             <  1:95266/138‑1 (MQ=255)

                                                                                                   aCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                             <  4:146785/138‑1 (MQ=255)

                                                                                                        atgaaaTAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                       <  5:294847‑M1/137‑4 (MQ=255)

                                                                                                        acgaaaTAATGCAATGAGTCGC‑TTTTTTA‑AATTTGGAAAGATACACGTAACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                       <  4:292983‑M1/137‑4 (MQ=255)

                                                                                                         ggaAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggt                                      <  5:176153‑M1/139‑5 (MQ=255)

                                                                                                          gaAATAATTCTATGAGTCGC‑GGTTTTT‑AAAATGGAAAATTACCACTGACTAAAGGGGATGGAGATTAATTATTTTCTTTAAGAAAGGCAGCATCCAAAAACCGAAAGGGGTCCCGCGCGCCTGGCGGGACTTTggggg                                      >  4:103190/1‑134 (MQ=255)

                                                                                                            agacagtctatgaGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                   <  7:226470‑M1/133‑8 (MQ=255)

                                                                                                            aataatTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                   <  7:283480‑M1/139‑8 (MQ=255)

                                                                                                                   cTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTTCTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                             <  5:100538‑M1/138‑14 (MQ=255)

                                                                                                                      tgatgcgc‑tttTATA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  4:26910‑M1/134‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTA‑AATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  2:39854‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  7:135448‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  1:789‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  6:272574‑M1/139‑18 (MQ=255)

                                                                                                                       tattcgc‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  1:140807‑M1/135‑18 (MQ=255)

                                                                                                                       gAGTCGC‑TTTTTTA‑AATTTGTAAAGTTACACGTTACTAAAGGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAATAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  5:234612‑M1/138‑18 (MQ=255)

                                                                                                                       gAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  1:246026‑M1/138‑18 (MQ=255)

                                                                                                                       gAGTCGA‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGATTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  1:58252‑M1/138‑18 (MQ=255)

                                                                                                                        aGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTTACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  6:175924‑M1/139‑20 (MQ=255)

                                                                                                                        aGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  7:54683‑M1/139‑20 (MQ=255)

                                                                                                                        aGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  1:16161‑M1/139‑20 (MQ=255)

                                                                                                                                tttttA‑ATTTTGTAACTTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  1:225484‑M1/138‑26 (MQ=255)

                                                                                                                                tttttA‑AATTTGGAAATTTACACGTTTCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACTGCTTCGAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  2:171889‑M1/138‑26 (MQ=255)

                                                                                                                                tttttA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  5:88084‑M1/138‑26 (MQ=255)

                                                                                                                                 ttttA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  7:244098‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttA‑AATTTGGAAAGGTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  4:124246‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttA‑AATTGGGAAAGTTACACGTTACTACAGGGACTGGAGATAAATTATTAGATATACTACTGCCAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  1:100264‑M1/137‑26 (MQ=255)

                                                                                                                                     a‑aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta            <  7:92023‑M1/138‑31 (MQ=255)

                                                                                                                                           tGGAAAGTTACACGTTACTAATGGGAATGGAGATAAATTATTAGATATAGTACTGACAGCTTACAAGTAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaataaggaagagtatgagtattcaa       <  6:98698‑M1/138‑36 (MQ=255)

                                                                                                                                           tGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaa       <  5:155603‑M1/138‑36 (MQ=255)

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  Exported/3603‑3882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`