BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,783	G→T	5.1%	intergenic (+68/‑88)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,783	0	G	T	5.1%	45249.1 / 304.0	13938	intergenic (+68/‑88)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (4093/8684); new base T (904/188); total (5025/8913)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 14335 total aligned reads displayed.

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG  >  Exported/3645‑3923

 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                              <  5:81879/138‑1 (MQ=255)

 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                              <  4:253786/138‑1 (MQ=255)

 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                                              <  1:92359/138‑1 (MQ=255)

      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTGTTTTATATGTATATTTAAAAGGTAAAAAAAGGAATGGATATAAATTAATTGataaa                                                                                                                                         >  8:15520/1‑136 (MQ=255)

               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                                                                 <  5:267174/137‑1 (MQ=255)

                 gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAAATTTGTAAGATAACCGGGAATAAAGGGGATTGAGAAAAAATAATAGATATAATAATGAAAGcg                                                                                                                             >  8:237514/1‑138 (MQ=255)

                  aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGTAAGTTTCACGGTTCTAAAGGGTATGGAGATTAATTTTTTTATATACTACTGACAGcat                                                                                                                            >  8:80573/1‑137 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                         <  3:133995/139‑1 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                         <  4:35840/139‑1 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                         <  4:82321/139‑1 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGACGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGGAGAGTTACACGTTTCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                         <  8:259391/139‑1 (MQ=255)

                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                                                        <  2:213612/139‑1 (MQ=255)

                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTTAACGGTACGAAAGGGAATTGTGTTAAATTATTTTATATTATAAAGACAGCTTGCa                                                                                                                         >  7:131857/1‑138 (MQ=255)

                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                       <  4:27921/139‑1 (MQ=255)

                       gTATTGTATATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                       <  6:46237/139‑1 (MQ=255)

                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGATGAAATAATTCTATGAGTAGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                       <  7:225195/138‑1 (MQ=255)

                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                      <  6:213246/139‑1 (MQ=255)

                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                      <  3:3002/139‑1 (MQ=255)

                          ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                                                                    <  3:114972/139‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                            <  1:159263/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                            <  6:263786/138‑1 (MQ=255)

                                       aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTTAACGGTTCTAAAGGGTTTTGTGATAAAATATTTTATTTAAAACTGCCAGCTTCCAAGAAGCGAAATAGGTgcc                                                                                                       >  5:61944/1‑139 (MQ=255)

                                        tAGTTATCTATTATTTACGGGGTGGTAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTAGTGACAGCTTCAAAGAAGCTAAAGAGGTCCCt                                                                                                      <  8:111718/139‑1 (MQ=255)

                                             aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGGAAGTTACACGTTTCGAAAGGGAATGGGGATAAATTATTTGATATACTTAGGACACCGTCCCAGCAGCGAAAGAGGTCCCGAgcgc                                                                                                 >  4:138272/1‑139 (MQ=255)

                                              agacagattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGcc                                                                                                <  3:276612/133‑1 (MQ=255)

                                                     tttAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTCCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                                                          <  5:123634/138‑1 (MQ=255)

                                                       tAACGGGAGGAATTAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGATTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGa                                                                                        <  5:142606/138‑1 (MQ=255)

                                                                gaAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAATTTTAAAC‑TTAATAAATGGTATGGAGATAAAATTTTATATAAAATAAAGAAACCTTACAAGAAAAAAAAGATGTACCAACAGCCTACGGGGAAattttggt                                                                               >  8:37549/1‑133 (MQ=255)

                                                                    taatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                                                          <  5:246361‑M1/139‑10 (MQ=255)

                                                                         cTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                      <  7:208209‑M1/138‑14 (MQ=255)

                                                                         cTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                                                      <  7:188902‑M1/138‑14 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  8:220314‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  1:139513‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACAGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  4:186585‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGA‑TTTTTTAAATTTGGAAAGTTTCACGTTACTAAAGTGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCATGAAGCAAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  7:258049‑M1/139‑18 (MQ=255)

                                                                             tagTCTC‑TTTTTTAAATTTGGAAAGTTACAAGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  5:185386‑M1/137‑18 (MQ=255)

                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  7:149308‑M1/138‑18 (MQ=255)

                                                                             gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                  <  6:286618‑M1/138‑18 (MQ=255)

                                                                              aGTCGA‑GTTTTTAAATTAGGAAAGATACACGTTACTAAAGGGAATGGATAAAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  8:280397‑M1/139‑20 (MQ=255)

                                                                               gTCGC‑TTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  1:183737‑M1/138‑20 (MQ=255)

                                                                               gTCGC‑TTTTTTAAATTTGGAAATTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  5:205623‑M1/138‑20 (MQ=255)

                                                                               gTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag                                                               <  1:50740‑M1/139‑21 (MQ=255)

                                                                               gTCGC‑TTTTATAAATTTGGAAAGTTACACGTTACTAAAGGGATTGTACATAAATTATTAGATATCCTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  2:237456‑M1/138‑20 (MQ=255)

                                                                               agtcctttttttaaatttGGAAAGTTACACGTTACATAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGACGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                <  4:208342‑M1/133‑20 (MQ=255)

                                                                                tCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATAAACTACTGACAGCTTCCACGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag                                                               <  6:6807‑M1/138‑21 (MQ=255)

                                                                                  gC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAATTTATTAGATATACTACTGACAGCTTCCTAGAAGCTAAAGAGGTCCCTAGCGCCTACGTGGAATTTGgggtacattgaaaaaggaatagt                                                            <  7:71605‑M1/139‑24 (MQ=255)

                                                                                     ttttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  1:203498‑M1/139‑26 (MQ=255)

                                                                                      tttttAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  3:214788‑M1/138‑26 (MQ=255)

                                                                                       ttttATATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  1:242758‑M1/137‑26 (MQ=255)

                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGTGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  2:288937‑M1/137‑26 (MQ=255)

                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  7:9041‑M1/137‑26 (MQ=255)

                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCCAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  6:121183‑M1/137‑26 (MQ=255)

                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  7:23925‑M1/137‑26 (MQ=255)

                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  2:46001‑M1/137‑26 (MQ=255)

                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  4:115577‑M1/137‑26 (MQ=255)

                                                                                       ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAAAGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                          <  1:31360‑M1/137‑26 (MQ=255)

                                                                                           aaaTTTTGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                                     <  3:236868‑M1/138‑31 (MQ=255)

                                                                                           aaaTTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                                     <  1:224320‑M1/138‑31 (MQ=255)

                                                                                            aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                   <  2:261727‑M1/139‑33 (MQ=255)

                                                                                            aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                   <  3:71731‑M1/139‑33 (MQ=255)

                                                                                                 ggAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaaca                                              >  2:141457‑M1/1‑102 (MQ=255)

                                                                                                  gAAAGTTACACGTTACTAAAGGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGCGGTCCCTAGCGCCTACGGGGAATTTGgtgtacattgaaaaaggaagagtatgagtattcaaca                                              <  4:23187‑M1/138‑38 (MQ=255)

                                                                                                          ctcgtTACTAAATGGAAGGGACATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                     <  1:278200‑M1/137‑47 (MQ=255)

                                                                                                          cacTTTACTAATGGGAATGGAGATAAATTATTAGATATACTACTGCCATCTTCCTAGATGCTAAAGAGGTCGCTAGCGCCTACGGGGAATTTGtggtagattgaaaaaggaagagtatgagtattcaacatttccgtgt                                     <  3:192926‑M1/139‑47 (MQ=255)

                                                                                                          cacGTTACTAAAGGTAATGGAGATAAATTATTAGAAATACAACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                     <  6:239644‑M1/139‑47 (MQ=255)

                                                                                                          cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAGGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                     <  1:100169‑M1/139‑47 (MQ=255)

                                                                                                          cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAATCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                     <  7:168970‑M1/139‑47 (MQ=255)

                                                                                                            cGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                   <  5:288270‑M1/139‑49 (MQ=255)

                                                                                                            agtTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                   <  6:163122‑M1/138‑49 (MQ=255)

                                                                                                             gTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgc                                  <  5:102695‑M1/139‑50 (MQ=255)

                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                               >  7:30552‑M1/1‑86 (MQ=255)

                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                               >  2:165425‑M1/1‑86 (MQ=255)

                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                >  1:119812‑M1/1‑86 (MQ=255)

                                                                                                                 cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                                                          >  4:160827‑M1/1‑86 (MQ=255)

                                                                                                                       ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccc                        >  1:72975‑M1/1‑80 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGTTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  6:189791‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGTTCACTAGCGCCTACGGGGAATTTGgggtaaattcaaaaaggaagagtatgagtattcaacatttccttgtcgccattattcactt                      >  3:51382‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtctcccttattccctt                      >  8:200092‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  6:92091‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  2:91334‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  2:67938‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  5:42458‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  8:177164‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  3:93095‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                      >  5:162335‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                     >  7:113699‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                                                     >  5:226000‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                                                        >  8:134876‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                >  4:60620‑M1/1‑78 (MQ=255)

                                                                                                                         aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                            >  7:51458‑M1/1‑78 (MQ=255)

                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                    >  2:190759‑M1/1‑76 (MQ=255)

                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                    >  6:141440‑M1/1‑76 (MQ=255)

                                                                                                                           tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctttt                    >  3:17188‑M1/1‑76 (MQ=255)

                                                                                                                              agaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctgattcccttttttg                 >  8:59348‑M1/1‑73 (MQ=255)

                                                                                                                                       attaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcatttt        >  3:254662‑M1/1‑64 (MQ=255)

                                                                                                                                       attaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcatttt        >  3:153043‑M1/1‑64 (MQ=255)

                                                                                                                                       attaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                            >  7:278572‑M1/1‑64 (MQ=255)

                                                                                                                                        ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                                                          <  2:11427/56‑1 (MQ=255)

                                                                                                                                        ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg       >  5:99769‑M1/1‑63 (MQ=255)

                                                                                                                                          aGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgcc     >  1:260462‑M1/1‑61 (MQ=255)

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTG  >  Exported/3645‑3923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`