BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	5,464	T→G	5.4%	intergenic (+733/‑424)	ampR → / → lacZalpha	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	5,464	0	T	G	5.4%	46410.0 / inf	21128	intergenic (+733/‑424)	ampR/lacZalpha	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base T (9882/9449); new base G (732/856); total (10774/10354)
Fisher's exact test for biased strand distribution p-value = 1.23e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 21220 total aligned reads displayed.

AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAG  >  Exported/5326‑5608

aaGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCt                                                                                                                                                  <  2:62932/139‑1 (MQ=255)

       aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                                           <  5:201747/139‑1 (MQ=255)

           gTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTGATGCTCGTCAgggggg                                                                                                                                       >  2:202820/1‑139 (MQ=255)

              tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCgg                                                                                                                                    >  1:17214/1‑139 (MQ=255)

              tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGGCAGGGGGGCg                                                                                                                                     <  7:75177/138‑1 (MQ=255)

              tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATATTTGTGATGCTCTTACGGGGGGccg                                                                                                                                    >  7:130970/1‑137 (MQ=255)

                     aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                                     >  8:57797/1‑131 (MQ=255)

                     aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAt                                                                                                                             >  1:21331/1‑139 (MQ=255)

                     aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTGATGCTCGTCAGGGGGGCGGAGCCGAt                                                                                                                             >  8:266621/1‑139 (MQ=255)

                          cAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCACCCGATGACGTGAGCGTCGAGTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTagtgaa                                                                                                                         >  3:224746/1‑133 (MQ=255)

                             ggTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAAc                                                                                                                     >  4:56813/1‑139 (MQ=255)

                                        gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                          <  7:94564/139‑1 (MQ=255)

                                        gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                          >  6:279148/1‑139 (MQ=255)

                                        gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                          <  4:126979/139‑1 (MQ=255)

                                            gcgcAAGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGcc                                                                                                      >  3:140603/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTTATTCTCGTCAGGGGGGCGGAGCCGATGGAAAAACGCCAGCAACGCGGccg                                                                                                     >  8:79761/1‑138 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGTTTCTCGGCAGGGGGGCGGAGCCGATGGTAAAACGCCCCCCACCCGGccc                                                                                                     >  2:282964/1‑138 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGaaa                                                                                                                        >  7:224999/1‑120 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCGCAACGCGGCCt                                                                                                     >  1:77157/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                                     >  7:18638/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                                     >  3:103760/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCGATGGAAAAACGCCAGCAACGCGGccg                                                                                                     >  1:57579/1‑138 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTGATGCTCGTCAGGGGGGCGGCGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                                     >  7:3863/1‑139 (MQ=255)

                                             agcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCTCCACCTCTTACTTGAGCGTCGAATTTTTTTATTCTCGTCAGGGGGGCCGAGCCTATGGAAAAACGCCACCAAACCGGccg                                                                                                     >  4:174204/2‑138 (MQ=255)

                                               cACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGGTTTGGGCAGCACGTCAGGGGGGCCGCGCCTAATGAAAAACGCCAGCCACGCGGCCatt                                                                                                   >  4:242160/1‑139 (MQ=255)

                                                 cGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGa                                                                                                                                   <  8:242726/105‑1 (MQ=255)

                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCgg                                                                                                                                    <  5:176739/103‑1 (MQ=255)

                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                                <  8:18117/139‑1 (MQ=255)

                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                                >  4:147847/1‑139 (MQ=255)

                                                   aGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                               <  8:41147/139‑1 (MQ=255)

                                                      gAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGCCTTGAGCGTCGATTTTTGTGTTTCTTCTCAGGGGGGCGGTGCCTATTGAAAAACGCCCGCAACGCGGCGGTTTTACGGt                                                                                            >  5:117672/1‑139 (MQ=255)

                                                       aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGtt                                                                                           >  1:39701/1‑139 (MQ=255)

                                                         cTTCCCGGGGGAAACGCCTTGTATCTTTATAGTCGTGTCGGGTTTCGCCACCTCTGACTTGCGCGGCGATTTTTGTGATGCTCGTCTGGGGGGGGGAGCCTATGTAAAAACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                                         <  1:25715/139‑1 (MQ=255)

                                                          ttCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                               >  5:260971/1‑132 (MQ=255)

                                                          ttCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCct                                                                                        >  3:279550/1‑139 (MQ=255)

                                                          ttCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCct                                                                                        >  4:281021/1‑139 (MQ=255)

                                                              aGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGTTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                                    <  5:278751/139‑1 (MQ=255)

                                                              aGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                                    >  6:117087/1‑139 (MQ=255)

                                                                   gAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTGATGCGGGGGAGGGGGGCGGGGCCTATGGAAAAACCCCCGCCACGCGGCCGTTTTAAGGGTCcgggccgtttg                                                                               >  7:108225/1‑139 (MQ=255)

                                                                    aaccGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGCGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCGCAACGCGGCCTTTTTACGGTTCcgggccgtttgg                                                                              >  7:264424/4‑138 (MQ=255)

                                                                    aaaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgc                                                                              >  7:20358/1‑139 (MQ=255)

                                                                          cTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTTATGGTGGTAATGGGGGCGGAGGCTATTGAAAAACGCCAGCAACCCCGCCTTTTTTCGGTTCcaggccatttgctggcct                                                                        >  4:80423/1‑139 (MQ=255)

                                                                                tCTTTTTAGTGCTGTCGGCTTTCGCCACCTCTGACTTGTGCGTCGA‑TTTTGTGTTGCTCGTCCGGGGGGCGGAGCTTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                                  <  1:100057/138‑1 (MQ=255)

                                                                                tCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGGAGCCAATGGAAAAACCCCAGCAACGCGGCCTTTTTACGGTTGCTggccgtttgctggccatttgct                                                                  >  2:250605/1‑139 (MQ=255)

                                                                                  tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTca                                                                >  3:70080/1‑139 (MQ=255)

                                                                                    tatgGTCCTGTCGGGGTCCGCCACCTGTGACTTGAGAGTCGTTTTTTTTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAc                                                               >  6:236963‑M2/1‑137 (MQ=255)

                                                                                    tatcggccTGGCGGGTTGCGCCACCTCTGCCTTGAGGGTCGATTTTTGTGATGCGCGTCTGGGGGGCGTAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTcac                                                               <  6:38378/132‑1 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCGGGCCTTTTGCTCAca                                                              <  1:153080‑M2/139‑3 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAc                                                               >  8:22171‑M2/1‑137 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTTTGATGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCGTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGGTgaca                                                              >  5:30047/1‑139 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTGCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAc                                                               >  3:51016‑M2/1‑137 (MQ=255)

                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggc                                                                          >  4:107990/1‑123 (MQ=255)

                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                          >  3:244593/1‑139 (MQ=255)

                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                          <  7:243472/139‑1 (MQ=255)

                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                          <  8:198976/139‑1 (MQ=255)

                                                                                             gTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACCCGGCCGTTTTACGGTTACTTGCCTTTTGGTGGCCTTTTGCTCACATGTTAttt                                                      >  2:160750/1‑138 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGt                                                                                                                                               >  6:187003/1‑48 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTcc                                                    >  5:220330/1‑139 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTcc                                                    >  3:261480/1‑139 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTAAGGGGGGCGGTGCCTAAGGAAAAACGCCAGCAACGCGGCCTTTTTTCGGTTGCTTGCCTTTTTCTGGGCTTTTGCTGACATGTTGtttac                                                    >  5:77280/1‑137 (MQ=255)

                                                                                                  gTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGGTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCg                                                <  6:259233/139‑1 (MQ=255)

                                                                                                   tttCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCGCCACGCGGCCGTTTTACGGGTCCTGGGCGTTTGCGGGCCGTTTGCTCACCTGTTCTTTCCTGCGt                                               >  8:160415/1‑139 (MQ=255)

                                                                                                   tttCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggccttttg                                                                    <  1:191469/118‑1 (MQ=255)

                                                                                                    ttCGCCACCTCTGACTTGAGCGGCGATTTTT‑TGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCCTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                             <  6:134585/139‑1 (MQ=255)

                                                                                                     tCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGGTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcg                                                                                                           >  2:226212/1‑77 (MQ=255)

                                                                                                      cGCCACTTCTGACTTGAGCGTCGATTTTTGTGTTGCTCGTCTGGGGGGCGGAGCCTTTGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                             <  3:159278/138‑1 (MQ=255)

                                                                                                      cGCCACCTCTGACTTGAGCTGCGATTTTTGTGTTGCTCGTCAGGGGGGCGGAGCCTAGGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                             <  4:99887/138‑1 (MQ=255)

                                                                                                      cGCCACCTCTGACTTGAGCGTGGATTTTTGTGATGCTGGTCAGGGGGGGGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                             <  3:29834/138‑1 (MQ=255)

                                                                                                      cGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                             <  4:218815/138‑1 (MQ=255)

                                                                                                      cGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                             <  3:205691/138‑1 (MQ=255)

                                                                                                       gCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGTTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATc                                           <  1:98841/139‑1 (MQ=255)

                                                                                                          aactctGAGTTGTGCGTCGATTTTTGTGATGCTCGTCCGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATcccc                                        <  6:156121/137‑1 (MQ=255)

                                                                                                            ctctGACTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTTTCCCCt                                       >  6:61296/1‑138 (MQ=255)

                                                                                                              ctGACTTGAGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGGTGGCCTTTTGCTAACATGTTGTTTCATGCCTTATACCCTGAt                                    >  6:212861/1‑139 (MQ=255)

                                                                                                               tGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAATAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAtt                                   <  5:133485/139‑1 (MQ=255)

                                                                                                               tGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAtt                                   <  3:121147/139‑1 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGa                            >  1:234868/1‑139 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGa                            >  7:53982/1‑139 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGa                            >  5:55546/1‑139 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGGGGTGCTCGGGAGGGGGGCGGAGGCGATGGAAAAACGCCAGCAACGCGGCGGTTTTACGGTTGCGGGCCGTTTTGTGGCCGTTTGCTGCCATGTTGATTCCTGCGTTATGCCCGGTTTCtggt                              >  4:258846/1‑135 (MQ=255)

                                                                                                                       gcggcgATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  6:84925/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGTGATGCTCGTCAGTGGGGCGGAGCCTATGGAAACACGCCAGCAACGAGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  2:182033/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  7:283864/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  3:183478/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  7:1679/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  7:149899/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  5:98682/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGGGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  1:281931/139‑1 (MQ=255)

                                                                                                                       gCGTCGATTTTTGGGATGCGCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  8:116451/139‑1 (MQ=255)

                                                                                                                       gCGTAGATTTTTTTGATGCTCGTCCGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           <  3:89238/139‑1 (MQ=255)

                                                                                                                        cGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                           >  2:82294/1‑138 (MQ=255)

                                                                                                                        cGTCGATTTTTGGGATGCTGGTCAGGGGGGCGGAGCCGATGGAAAAACTCCAGCCACGCGGCCTTTTTACGGTTCCTGGCCGATTGCTGGCCGTTTGCTCCCATTTTCTTTACGTCCTTATCCCCGGATGCGGTGGAt                           >  8:288319/1‑138 (MQ=255)

                                                                                                                         gTCGTTTTTTGTGATGCGCGTCTGGGGGGCGGAGCCTATGCAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa                         <  1:124628/139‑1 (MQ=255)

                                                                                                                           cGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAcc                       >  2:4349/1‑139 (MQ=255)

                                                                                                                           cGATTTTTGTGAGGCGCGTCAGGGGGGCGGAGCCTAGGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAcc                       <  5:152384/139‑1 (MQ=255)

                                                                                                                           cGATTTTGGTGATGCTCGGCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAcc                       <  4:199366/139‑1 (MQ=255)

                                                                                                                                  tgtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAATACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa                 <  5:240720/138‑1 (MQ=255)

                                                                                                                                  tgtgATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa                 <  4:33980/138‑1 (MQ=255)

                                                                                                                                  tgtgATGCTCGGCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa                 <  2:129564/138‑1 (MQ=255)

                                                                                                                                        gCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCtt          >  8:139676/1‑139 (MQ=255)

AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAG  >  Exported/5326‑5608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`