BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,033	A→C	5.0%	H49P (CAT→CCT)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,033	0	A	C	5.0%	57083.7 / inf	21157	H49P (CAT→CCT)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (7902/11675); new base C (1371/123); total (9289/11868)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 22204 total aligned reads displayed.

TGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAT  >  Exported/5895‑6171

 gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACAt                                                                                                                                            >  4:77883/1‑139 (MQ=255)

 gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACAt                                                                                                                                            >  7:248183/1‑139 (MQ=255)

  aTTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATc                                                                                                                                           <  1:28100/139‑1 (MQ=255)

      cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATcccc                                                                                                                                        >  7:18681‑M2/2‑138 (MQ=255)

      cGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTTGGAAAACCCGGGCGGTACCCAACATAATAG‑CCATGAAGCACATccccc                                                                                                                                       >  7:225556‑M2/2‑139 (MQ=255)

                 cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                             <  1:210016/138‑1 (MQ=255)

                 cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                             <  6:225753/138‑1 (MQ=255)

                 cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                             <  7:84204/138‑1 (MQ=255)

                 cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                                             <  1:75814/138‑1 (MQ=255)

                       tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACCTAGGGTCGGGGAAAACCCGGGCGGTAACCCACCTAAACGCCCTTGC‑GCACCTCCCCCAATAGCCACCAGGCggt                                                                                                                      >  6:6627/1‑137 (MQ=255)

                       tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGGCTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCCGCCCATCCCCCGTTCCCCAGCTGGGG‑t                                                                                                                      >  1:97173/1‑138 (MQ=255)

                       tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAACCCCTGGCGTTACCCAACTTAATCG‑CCTTGCCGCACATCCCCCGTTCGCCCGCTGGCG‑t                                                                                                                      >  8:168654/1‑138 (MQ=255)

                       tGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑t                                                                                                                      >  7:169262/1‑138 (MQ=255)

                         cAGGTCGACTCTTGAGGATCCCAGTGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGTGAAAAACCTGGCGTTACCCATCTTTATCT‑CCTTGCAGCACTTCCCCGTTTCGCCAGATTGCG‑Taa                                                                                                                    <  8:226283/138‑1 (MQ=255)

                         cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑Taa                                                                                                                    <  6:131843/138‑1 (MQ=255)

                               gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGaa                                                                                                             <  3:248781/139‑1 (MQ=255)

                               gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGaa                                                                                                             <  3:12113/139‑1 (MQ=255)

                               gACTCTAGAGGATCCCCGGGTACCGAGCTCGAAATCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCGGGCG‑TAATAGCGaa                                                                                                             <  2:155551/139‑1 (MQ=255)

                               gACTCTAGAGGAGCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGaa                                                                                                             <  3:205213/139‑1 (MQ=255)

                                       agGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCg                                                                                                     <  5:150497/139‑1 (MQ=255)

                                           tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCTGGCGTTACCCCACTTAATCG‑CCGTGCAGCCCCTCCCCCGTTCGCCCGCGGGCG‑GAATAGCGCAGAGGCCCGcccc                                                                                                 >  1:54528/1‑139 (MQ=255)

                                           tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGcccc                                                                                                 >  7:4354/1‑139 (MQ=255)

                                           tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACAGGGAAAACCCTTGCGTTACCCAACTTAATCG‑CCATTCAGCACATCCCCCATTCGCCCGCTGGCG‑TAATAGCGAATAGGCCCGCAcc                                                                                                 >  8:150420/1‑139 (MQ=255)

                                           tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGCCTGGGTAAACCCTGGCGATACCCAACTTAATAG‑CCATGCCGCCCCTCCCCCTGTGGAAAGCTGGCG‑TTATTACGAAGAGGCCCGcccc                                                                                                 >  2:278043/1‑139 (MQ=255)

                                            tcccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACGGGGAAAACCCTGGCGGTACCCCACATAAACG‑CCATGCAGCCCATCCCCCATTCGCCACCTGGCG‑GAATAGCGAAGAGGCCCGCCCCg                                                                                                >  3:182891/2‑139 (MQ=255)

                                            ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCG‑CCTTGCAGCCCAGCCCCCTTTCGCCAGCGGGCG‑GTATTACGCAGCGGCCCGCACCg                                                                                                >  4:127560/1‑139 (MQ=255)

                                            ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCAcc                                                                                                 >  4:220476/1‑138 (MQ=255)

                                            ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAATGGGAAAACCCTGGCGTTACCCAACTTAAACT‑CCTTGCAGCACATACCCCATTCGCCAGCAGGCG‑TAATATCGAAGAGGCCCGCAccc                                                                                                >  5:171144/1‑138 (MQ=255)

                                            ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACCTCGTGACTGGGCAAACCCAGGCGTTACCCAACTTAATAG‑CCATGCCGCACCTACCCCTTTAGCCACCTGGCG‑GAAAAGCGAAGAGGCCCCCACCg                                                                                                >  2:196775/1‑139 (MQ=255)

                                                gggTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCg                                                                                            <  8:41401/139‑1 (MQ=255)

                                                  gTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGcc                                                                                          <  4:279538/139‑1 (MQ=255)

                                                  gTACCGAGCTCGAATGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGcc                                                                                          <  3:66773/139‑1 (MQ=255)

                                                   tACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGACGAGGCCCGCACCGATCGccc                                                                                         <  1:255718/139‑1 (MQ=255)

                                                   tACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGccc                                                                                         <  2:3785/139‑1 (MQ=255)

                                                        aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATCC‑CCTTGCCGCCCATCCCCCATTCCCCAGCTGGCG‑GAATACCGAAGAGGCCCGCCCCCCTCGcccgaacc                                                                                    >  3:28762/1‑134 (MQ=255)

                                                        aGCTCGAATGCACTGGCCGTCGATTAACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCACCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                                    <  6:112723/139‑1 (MQ=255)

                                                                   aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                         <  8:204595/139‑1 (MQ=255)

                                                                   aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                         <  8:245168/139‑1 (MQ=255)

                                                                   aCTGGCCGTCGTTTTACAACGGCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGGG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                         <  3:160224/139‑1 (MQ=255)

                                                                   aCTGGCCGGCGTTTTACAACGGCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                                         <  1:147458/139‑1 (MQ=255)

                                                                    cTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGGGTTTCCCCACTTAATAG‑CCTTGCAGCACAACCCCCGTTAGCCAGCGGGGG‑TAATAGCGAAGAGGCCCGCCCCGATCGGCCTTCCCAACACTTgcccc                                                                        >  5:13161/1‑136 (MQ=255)

                                                                        ccGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCt                                                                    <  5:114947/139‑1 (MQ=255)

                                                                            cgtTTTACAACGTCGTGACAGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCCGCCCCTCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGCTCGCCCTTCCCCACAGTTGCGCAGCCGgaat                                                                >  1:165104/1‑139 (MQ=255)

                                                                                      cctcgtGACTGGGAAAACCCAGGCGTTTCCCAACTTAATAC‑CCGTGCAGCACATCCCCCGTTAGCCAGCAGGCGTTAATA‑CGAAAAGGCCCCCCCCGATCCCCCAACCCAACACTTTCGCAGCCTtaatggcgaatggc                                                      >  2:56761/3‑139 (MQ=255)

                                                                                        tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCg                                                                                            >  8:81666/1‑99 (MQ=255)

                                                                                        tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                    >  1:38051/1‑139 (MQ=255)

                                                                                        tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                    <  5:295488/139‑1 (MQ=255)

                                                                                        tcgtGACTGGGAAAACCCTGGCGTTACCCAACATAATCG‑CCATGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGAACGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                                    >  2:77435/1‑139 (MQ=255)

                                                                                         cgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGTCGcc                                                   >  5:273677/1‑139 (MQ=255)

                                                                                           tGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTg                                                 >  6:87475/1‑139 (MQ=255)

                                                                                             aCTGGGAAAACCCTGGCGTTACCCAAATTAATAG‑CCTTGCATCACATCCCCCTTTCGCCCGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTGCCAACAGTTTCGCAGCCGGAATGGCGAATTGCGCCTGAt                                               >  4:261288/1‑139 (MQ=255)

                                                                                                gggAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACCTCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCg                                            <  1:231672/139‑1 (MQ=255)

                                                                                                     aaCCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtt                                       <  6:247948/139‑1 (MQ=255)

                                                                                                     aaCCCTGGCGTTAACCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCT‑TAATAGCGAAGATGCCCGCACAGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtt                                       <  4:197541/139‑1 (MQ=255)

                                                                                                       cccTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                     <  3:267540/139‑1 (MQ=255)

                                                                                                         cTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct                                   <  7:131977/139‑1 (MQ=255)

                                                                                                           ggCGTTACCCAACTTAATCG‑CCTAGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                     >  1:122127/1‑135 (MQ=255)

                                                                                                           ggCGTTACCCAACTTAATCG‑CCGTGCAGCACAGCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc                                 <  2:247662/139‑1 (MQ=255)

                                                                                                              gTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                              <  5:53905/139‑1 (MQ=255)

                                                                                                              gTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                              <  7:205863/139‑1 (MQ=255)

                                                                                                              gTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                              <  5:254886/139‑1 (MQ=255)

                                                                                                              gTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTa                              <  8:103/139‑1 (MQ=255)

                                                                                                                 aCCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                            <  6:284783/138‑1 (MQ=255)

                                                                                                                 aCCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg                            >  8:61017/1‑138 (MQ=255)

                                                                                                                   ccAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                         <  7:152436/139‑1 (MQ=255)

                                                                                                                      aCTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                                     >  8:290338/1‑124 (MQ=255)

                                                                                                                       cTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcg                                                                          <  6:150592/86‑1 (MQ=255)

                                                                                                                       cTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCTCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgt                     >  2:124181/1‑139 (MQ=255)

                                                                                                                       cTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgt                     >  5:73954/1‑139 (MQ=255)

                                                                                                                       cTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgt                     >  3:86300/1‑139 (MQ=255)

                                                                                                                          aaTCG‑GCTTGCATCACCTCGCCCTTTCTCCAGGTGGCG‑TAATAGCGATGAGGCCCGCACCGATCGCCCTTCGCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTGCTTACGCATCtgtg                    <  4:98872/137‑1 (MQ=255)

                                                                                                                          aaTCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                    <  3:44191/137‑1 (MQ=255)

                                                                                                                          aaTCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                    <  8:185294/137‑1 (MQ=255)

                                                                                                                          aaTCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                    <  5:213134/137‑1 (MQ=255)

                                                                                                                          aaTCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                  <  4:105519/139‑1 (MQ=255)

                                                                                                                          aaTCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACACTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                    <  7:270545/137‑1 (MQ=255)

                                                                                                                           aTCC‑CCTTGCAGCACATCCCACTTTCGCCAGCGGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                  <  3:100544/138‑1 (MQ=255)

                                                                                                                                  ttGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt                         >  7:290484/1‑125 (MQ=255)

                                                                                                                                   tGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTca          >  7:62267/1‑139 (MQ=255)

                                                                                                                                    gcagccCATCCCCCTATCGCCAGCTGGCG‑TAACAGCGCAGCCGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCGGAAGGGCGAATGGCGCCTGATGCGGTATTCTCTCCTTACGCATCTGTGCGGTATTTcac         <  6:265341/139‑1 (MQ=255)

                                                                                                                                    gcagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTca          <  8:129179/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCGTTGTGCCACCTGTTG‑TAATAGCGAAGAGGCCCGCACCGATCGACCTTCCCTACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACTCATCTGTTCGGTATTTcac         <  6:46007/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCTGCTGGCG‑TAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  6:174699/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  2:175655/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGCGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcaca        <  5:93885/139‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCtgtg                    >  7:108979/1‑127 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcaca        <  1:1878/139‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  1:100087/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  8:269171/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  2:2213/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  3:140749/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  3:227264/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  5:235571/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  4:243386/138‑1 (MQ=255)

                                                                                                                                     cagcaCATCCCCCTTACGCCAGCTGGCG‑TAATAGCGACGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  5:193459/138‑1 (MQ=255)

                                                                                                                                     cagcaCAGCCCCCTTTCGCCATCTGGCG‑TAATAGCTATCAGGCCCGCACCGATCGCCCTTCCCAACAGTGGCTCAGCCTGAATGGCGAATGGCGCCTGATTCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcag         <  4:31926/138‑2 (MQ=255)

                                                                                                                                          caTCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCg                  >  4:55163/1‑124 (MQ=255)

                                                                                                                                           aTCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTcac         <  4:173171/132‑1 (MQ=255)

                                                                                                                                           aTCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCat  <  4:146588/139‑1 (MQ=255)

                                                                                                                                           aTCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCa   >  6:202385/1‑138 (MQ=255)

TGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCG‑CCTTGCAGCACATCCCCCTTTCGCCAGCTGGCG‑TAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCAT  >  Exported/5895‑6171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`