BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,256	A→C	5.4%	intergenic (+45/–)	lacZalpha → / –	/vntifkey=4/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,256	0	A	C	5.4%	46411.0 / inf	19493	intergenic (+45/–)	lacZalpha/–	/vntifkey=4/–
Reads supporting (aligned to +/- strand): ref base A (8832/8956); new base C (1303/67); total (10430/9063)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 19853 total aligned reads displayed.

GCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTT  >  Exported/6118‑6394

gcgcCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGa                                                                                                                                            <  4:29555/139‑1 (MQ=255)

       aTGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGc                                                                                                                                       <  2:60360/137‑1 (MQ=255)

            gTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTg                                                                                                                                <  2:230406/139‑1 (MQ=255)

            gTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGAAGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTg                                                                                                                                <  3:140036/139‑1 (MQ=255)

                      cTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCa                                                                                                                       <  1:63378/138‑1 (MQ=255)

                              tCTGTGCGGTATTTCACCCCGCATATGGTGCACTCTCAGTACACTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGCCGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCTTAc                                                                                                              >  7:209708/1‑139 (MQ=255)

                                tgtgCGGTATTTCCCACCGCATATGGTGCCCTCTCAGTACAATCTTCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTATGCGACCGGCGACCCCTTTCAg                                                                                                            >  7:190306/1‑139 (MQ=255)

                                tgtgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCCCTTa                                                                                                               >  3:120965/1‑136 (MQ=255)

                                  tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGGTTGTGTGCTCCCGGCCTACCCTTACAGAc                                                                                                          >  6:23597/1‑139 (MQ=255)

                                  tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                          <  5:133011/139‑1 (MQ=255)

                                    cGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACa                                                                                                         <  1:215498/138‑1 (MQ=255)

                                     ggTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAg                                                                                                       <  7:109606/139‑1 (MQ=255)

                                       tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTACGCCAGCCCCGACACCCGCCACCACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGCCCAGCt                                                                                                     >  6:119509/1‑139 (MQ=255)

                                       tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACACGCt                                                                                                     >  6:290970/1‑139 (MQ=255)

                                       tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACCGGCAAGTCTTCTACCCGCATCCGCTTACAGACAAGCt                                                                                                     >  4:253576/1‑139 (MQ=255)

                                            cacaCCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTATGCTCCCGGCATCCGCTTACAGACAAGCTGGGAc                                                                                                >  2:40955/1‑139 (MQ=255)

                                            cacaCCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCAAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                 <  6:186717/138‑1 (MQ=255)

                                             acacCGCATATGGTTCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAc                                                                                                <  8:163803/138‑1 (MQ=255)

                                              cacCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCg                                                                                              <  2:278309/139‑1 (MQ=255)

                                                ccGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCAt                                                                                                                      <  8:224232/113‑1 (MQ=255)

                                                 cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                 <  7:250899/133‑1 (MQ=255)

                                                 cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGtct                                                                                           >  4:137993/1‑139 (MQ=255)

                                                 cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCCTCCGCTTACAGACAAGCTGGGACCgggg                                                                                           >  2:183731/1‑136 (MQ=255)

                                                 cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACCGGCTTGTCAGCTCCCCGCATCCGCTTACAGACAACCTGGGACCctct                                                                                           >  1:44807/1‑139 (MQ=255)

                                                   cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCt                                                                                                                 <  1:289484/115‑1 (MQ=255)

                                                   cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                         >  7:29252/1‑139 (MQ=255)

                                                   cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                         >  6:100094/1‑139 (MQ=255)

                                                      atGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGCCGGGCGTGTCTGCTCCCCGCCTCCGCTTACAGCCAAGCTGTGACCGTCTCCcgg                                                                                      >  4:72776/1‑139 (MQ=255)

                                                       tGGTGCACTCTCAGTCCAATCTGCTCTGATGACGCATAGTGAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                      <  6:68707/138‑1 (MQ=255)

                                                       tGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                      <  4:92241/138‑1 (MQ=255)

                                                         gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGTCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                    <  5:243493/138‑1 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                               <  8:102379/109‑1 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                           >  7:149990/1‑113 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                      <  7:86632/134‑1 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTg                                                                                 >  3:76513/1‑139 (MQ=255)

                                                           gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCAGCTCCCGGCATCCGCTTACAGACAATCTGTGACCGTCTCCGGGAGCTg                                                                                 >  5:279724/1‑139 (MQ=255)

                                                            cACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTg                                                                                 >  2:8313/1‑138 (MQ=255)

                                                            cACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCGGCTCCCCGCCTCCGCTTACAGACAAGCTGTGCCCGTCTCCGGGCGCGGc                                                                                >  4:275209/1‑139 (MQ=255)

                                                             gctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                               <  5:221712/138‑1 (MQ=255)

                                                             aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                               <  3:95228/139‑1 (MQ=255)

                                                              ctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCACCACCCGCTGACGCGCCCAGACGGGCGTGTATGCTCCCGGCATACGCTTAAAGCCCAGCTGTGCCCCTCGGCGGGCGCGGccg                                                                              >  3:162130/1‑137 (MQ=255)

                                                                    gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                         >  3:268243/1‑122 (MQ=255)

                                                                    gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATgtgt                                                                          >  2:163173/1‑137 (MQ=255)

                                                                    gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCa                                                                        <  3:246333/139‑1 (MQ=255)

                                                                    gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACGGGCTTGTCAGCACCCGGCATCCGCATACAGCCAAGCTGTGACCGTCTCCGGGAGCTGCATgtgt                                                                          >  3:57149/1‑137 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                           <  1:238497/99‑1 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGt                                                                   >  5:261625/1‑139 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCag                                                                       >  8:205774/1‑135 (MQ=255)

                                                                         aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCGTGTCTGCTCCCGGCAACCGCTTACAGCCAAGCTGTGACCGTCTCCGGGGGCGGCAAGTGTCAGAGGt                                                                   >  7:273857/1‑139 (MQ=255)

                                                                           cTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGACCGGCTTGTCTGCTACCCGCCTCCGCATACACACAACCTGTGACCGTCTCCGGGCGCTGGAGGTGTCAGAGGttt                                                                 >  2:130965/1‑139 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACa                                                                                                         <  5:175594/97‑1 (MQ=255)

                                                                             gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                 >  6:211957/1‑105 (MQ=255)

                                                                              ctctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCa                                                              <  5:36678/139‑1 (MQ=255)

                                                                               tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCACCACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGCGCTGCATGTGTCAGAGGTTTTCAc                                                             >  7:170999/1‑139 (MQ=255)

                                                                                   aTGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCATACAGACAAGCTGTGACCGTCTCCCGGAGCGGCATGTGTCAGAGGGTTTCACCGt                                                          >  4:188558/1‑138 (MQ=255)

                                                                                   aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGt                                                          >  1:193760/1‑138 (MQ=255)

                                                                                   aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGt                                                          >  7:67839/1‑138 (MQ=255)

                                                                                        gCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCGCCCGGCCTCCCCTTACAGACAAGCTGTGCCCCTCTCCGGGAGCGGCCTGTGTCAGAGGGTTTGACCGtcctccc                                                    >  8:28840/1‑137 (MQ=255)

                                                                                          aTAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCg                                                  <  7:48294/139‑1 (MQ=255)

                                                                                              ttAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGTCCGTCTCCGGGAGCTGCATGTTTCAGAGGTTTTCACCGTCATCACCCAAAc                                              >  3:38989/1‑139 (MQ=255)

                                                                                               tAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCCTCCCCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcg                                             >  7:230686/1‑139 (MQ=255)

                                                                                                aaGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                               >  4:53068/1‑72 (MQ=255)

                                                                                                 agccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACg                                                                                                                                          >  8:144440/1‑44 (MQ=255)

                                                                                                 agccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGt                                                          <  4:210673/124‑1 (MQ=255)

                                                                                                 agccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcgcg                                           <  8:246588/139‑1 (MQ=255)

                                                                                                   ccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTAAAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcgcgcg                                         <  4:232228/139‑1 (MQ=255)

                                                                                                    cagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCga                                        <  4:163993/139‑1 (MQ=255)

                                                                                                    cagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCga                                        <  3:226991/139‑1 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGGCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTGTCCGGGAGCGGCATGTGTCAGAGGTTTTGACCGTCATCCCCGCAACGGGCgcga                                      >  8:43279/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                      >  7:131479/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCt                                                                                                                 >  6:158431/1‑64 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                      >  5:58906/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                      >  3:285085/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGGCATCACCGAAACGCGCgaga                                      >  8:270240/1‑139 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCCGCATCCGCTTACAGACAAGCGGTGACCGTCTCCGGGAGCTGCAAGTGTCAGAGGGTTTCCCCGTCCTCACCCAAAcgcgcgggc                                      >  6:89489/1‑136 (MQ=255)

                                                                                                      gccCCGACACCCGCCAACACCCGCTGACGCGCCCAGACCGGCGTGTCTGCTCCCCGCATCCCCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGGAAGTGTCAGAGGTTTTCACCGGCCTCACCCAAACCCGCcagc                                      >  2:206164/1‑138 (MQ=255)

                                                                                                       ccccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGCGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGAc                                     >  5:80288/1‑139 (MQ=255)

                                                                                                       ccccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGAc                                     >  1:173144/1‑139 (MQ=255)

                                                                                                       ccccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCCAAACGCGCGAGAc                                     >  2:102535/1‑139 (MQ=255)

                                                                                                        cccGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCTCCCGGCCTCCGCGTTAAGCCAACCTGTGACCCTCGCCGGGCGCTGCATGGGTCGGAGGTTTTCACCGTGCTCCCCCGAAcgcgcggggcg                                    >  6:13816/1‑134 (MQ=255)

                                                                                                          cGACACCCGCCAACAGCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGaa                                  <  1:100150/139‑1 (MQ=255)

                                                                                                            ACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGGTTGTATGCTCCCGGCCTACGCTTTCAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGGCAGAGGTGTTCACCGTGATCACCGAAACGCGGGAGACGAAAg                                <  6:49699‑M1/139‑1 (MQ=255)

                                                                                                                CCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGcc                            <  1:152614‑M1/139‑1 (MQ=255)

                                                                                                                 CGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                 <  1:84341‑M1/69‑1 (MQ=255)

                                                                                                                 CGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCt                           >  5:113453‑M1/1‑139 (MQ=255)

                                                                                                                 CGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCGGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGATGTTTTCACCGTCATCACCTAAACGCTCGAGACGAAAGGGCCt                           >  5:152223‑M1/1‑139 (MQ=255)

                                                                                                                  GCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                          <  6:255462‑M1/59‑1 (MQ=255)

                                                                                                                  GCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGt                                                                   >  4:116216‑M1/1‑98 (MQ=255)

                                                                                                                      acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                      <  3:209293/139‑1 (MQ=255)

                                                                                                                           cGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGc                 <  6:272947/139‑1 (MQ=255)

                                                                                                                            gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCCCCGCAACGCGCGAGACGAAAGGGCCTCGTGATACGcc                >  8:64482/1‑139 (MQ=255)

                                                                                                                            gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGcc                >  3:18738/1‑139 (MQ=255)

                                                                                                                            gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCGGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGcc                >  2:250809/1‑139 (MQ=255)

                                                                                                                                   cgcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGACACGCGCGAGACGAAAGGGCCTCGTGATACGCCTAttttt         <  3:100698/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACATGCTGTGACCGTCTCCGGGTGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACTCGCGAGACTATAGGGCCTCGTGATAAGCCTATTTTta        <  8:185110/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  8:123631/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        <  5:20276/139‑1 (MQ=255)

                                                                                                                                    gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCGCGTGATACGCCTATTTTta        <  1:125710/139‑1 (MQ=255)

                                                                                                                                     cccTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCTAACCGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTtat       <  1:26223/139‑1 (MQ=255)

                                                                                                                                        tGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta        >  8:84184/1‑135 (MQ=255)

GCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTT  >  Exported/6118‑6394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`