BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,277	A→C	5.3%	intergenic (+66/–)	lacZalpha → / –	/vntifkey=4/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,277	0	A	C	5.3%	61056.8 / inf	21953	intergenic (+66/–)	lacZalpha/–	/vntifkey=4/–
Reads supporting (aligned to +/- strand): ref base A (9746/10593); new base C (1527/40); total (11298/10655)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 22159 total aligned reads displayed.

CCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTT  >  Exported/6139‑6415

  ttACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                          >  3:159239/1‑139 (MQ=255)

  ttACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                          >  7:208071/1‑139 (MQ=255)

          cTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACa                                                                                                                                  <  2:225781/139‑1 (MQ=255)

             tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                >  4:70546/1‑138 (MQ=255)

             tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                >  6:86457/1‑138 (MQ=255)

             tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                                               <  5:171607/139‑1 (MQ=255)

               cGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACaa                                                                                                                             >  2:37440/1‑139 (MQ=255)

                  tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCt                                                                                                                          >  4:117369/1‑139 (MQ=255)

                       cacaCCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGACAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                                      <  6:267694/138‑1 (MQ=255)

                            cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTTCTCCCGGCATCCGCTTACAGACACGCTGTGACCGtct                                                                                                                >  3:263954/1‑139 (MQ=255)

                            cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACa                                                                                                                              <  4:208777/125‑1 (MQ=255)

                              cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                                              <  6:155341/139‑1 (MQ=255)

                              cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                                              >  6:204693/1‑139 (MQ=255)

                                  tGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                                           <  5:110712/138‑1 (MQ=255)

                                  tGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                                           <  1:83419/138‑1 (MQ=255)

                                    gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                                         <  1:149567/138‑1 (MQ=255)

                                    gTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGGCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGc                                                                                                        <  7:4779/139‑1 (MQ=255)

                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                                                    >  7:10560/1‑109 (MQ=255)

                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAg                                                                                                                            <  3:28238/117‑1 (MQ=255)

                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTg                                                                                                      >  4:137596/1‑139 (MQ=255)

                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCCGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGACCTg                                                                                                      >  7:228652/1‑139 (MQ=255)

                                      gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCGCCCGGCATCCGCTTACAGCCAAGCTGTGACCGTCTCCGGGCGCTg                                                                                                      >  8:123266/1‑139 (MQ=255)

                                       cACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                                      <  1:189891/122‑1 (MQ=255)

                                         ctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCt                                                                                                                                      >  6:116752/1‑104 (MQ=255)

                                            tcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                                         >  5:277718/1‑130 (MQ=255)

                                             cAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAGCACCCGCTGACGCGCCCTGGCCGGGTTGTCTGCGCCCGGCAACCGCGTAGAGGCAAGCTGGGACCGGCGCCGGGCGGTGCCtgttg                                                                                               >  4:295155/1‑137 (MQ=255)

                                              aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGGCCGGCTTGTCTGCTCCCGGCCTACGCATACAGACAAGCGGGGACCCGCTACGGGAGCTGGCTgtgt                                                                                               >  3:205089/1‑138 (MQ=255)

                                              aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATgtgt                                                                                               >  4:186337/1‑138 (MQ=255)

                                              aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCGTACAGACAAGCTGTGACCGTCGCCGGGAGCTGCATGTGTc                                                                                              >  3:180776/1‑139 (MQ=255)

                                               gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATgtgt                                                                                               >  1:168919/1‑137 (MQ=255)

                                               gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCa                                                                                             <  1:284239/139‑1 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGGTTTTATGCTCCCCGCAACCGCATAACGACAACCAGTGCCCGGCTCCCGGAGCTGCATGTGTCAGAggg                                                                                        >  7:12788/1‑138 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                                         >  3:74914/1‑122 (MQ=255)

                                                    aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGt                                                                                        >  1:12344/1‑139 (MQ=255)

                                                    aTCTGCCCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                                           <  8:162201/120‑1 (MQ=255)

                                                     tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGTCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtt                                                                                       >  6:134235/1‑139 (MQ=255)

                                                     tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                >  2:77780/1‑98 (MQ=255)

                                                     tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtt                                                                                       >  8:268238/1‑139 (MQ=255)

                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTCACGCGCCCTGACCGGCTTGTCTGCAACCCGCATCAGCATACAGCCACCCAGGCACCGTCTCCGGGAGCAGCCTGGGTCagagcgttt                                                                                     >  3:223494/1‑133 (MQ=255)

                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTACCGGCATCCGCTTACAGCCAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGgttt                                                                                     >  8:205153/1‑138 (MQ=255)

                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTGAGAGGGTTTc                                                                                    >  4:274104/1‑139 (MQ=255)

                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCCCTTACAGACAAGCTGTGCCCGTCTCCGGGAGCGGCATGTGTCAGAGGgttt                                                                                     >  7:189158/1‑138 (MQ=255)

                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCATGTCTGCTCCCGGCCTACGCTTACAGCCAAGCTGTGCACGTCACCGGGCGCGTCATGTGTCAGAGGtttt                                                                                     >  8:246048/1‑138 (MQ=255)

                                                        gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCAGCCGGGCTTGTATGCTCCCCGCAACCGCATACAGACCAGCTGTGACCGGCTCCGGGAGCTGCATGTGTAAGAGGgttt                                                                                     >  4:50919/1‑138 (MQ=255)

                                                          tctTATGCCGCATAGTTAAGCCAGCCCCGACCCCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCCTCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTAAc                                                                                  >  5:56780/1‑139 (MQ=255)

                                                          tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTTTTCTCCCGGCATCCGCTTACAGa                                                                                                                                >  8:83172/1‑93 (MQ=255)

                                                          tctGATGCCGCATAGTTAAGCCAGCCCCGACACCAGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCACCCCGCATCCGCTTACAGACAAGCTTTGACCGTCGCCGGGAGCTGCATGTGGAACAGGTTTTCAc                                                                                  >  7:273436/1‑139 (MQ=255)

                                                             gATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCAGCACCCGGCCTCCGCTTACACACAACCAGTGACCGTCTCCGGGAGCTGCATGTTTCAGAGGTTTTCACCg                                                                                >  5:150691/1‑138 (MQ=255)

                                                                   gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCAc                                                                         >  7:292248/1‑139 (MQ=255)

                                                                   gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCCCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGtcatacc                                                                         >  5:240995/1‑136 (MQ=255)

                                                                      tAGTTAAGCCAGCCCCGACTCCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACACACAACCTGTGACCGTCTCCGGGAGCTGCATTTGTCAGAGGTTTTAACCGTCATAAccc                                                                       >  8:62666/1‑137 (MQ=255)

                                                                        gTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCCGGCTTGTCTGCTCCCGGCATCCGCTTAAAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTGACCGTCATCACCGcaa                                                                    >  1:212913/1‑139 (MQ=255)

                                                                        gTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGaa                                                                     <  4:90297/138‑1 (MQ=255)

                                                                              ccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcgcgcg                                                              >  8:142923/1‑139 (MQ=255)

                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGTCGGGCTTGTCTGCTCCCGGCATACGCTTACAGCCAAGCGGTGACCGTCTACGGGAGCCGCAGGTGTCAGAGGTTTTCACCGTCATAACCGAACCGAGCgaga                                                           >  7:66712/1‑139 (MQ=255)

                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCTTCCGCt                                                                                                                                      <  2:200118/64‑1 (MQ=255)

                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                                      >  4:161325/1‑80 (MQ=255)

                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                                                    <  5:219257/98‑1 (MQ=255)

                                                                                 gccCCGACACCCGCCAACACCCGCTGACGCGCCCGGCCGGGCGTGTATTCTCCCCGCCTCCGCGTAAAGACCAGCAGGTACCGGCGCCGGGGGCTGCATGTGTCAGCGGTTTTGAACGGGCTCACCGCAAcccgcgggc                                                           >  2:97470/1‑136 (MQ=255)

                                                                                  ccccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCt                                                                                                                                      <  5:97155/63‑1 (MQ=255)

                                                                                   cccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCGGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGAc                                                          >  1:25799/1‑138 (MQ=255)

                                                                                     cGACACCCGCCAAGACCCGCGGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGCCCGGCTCCGGGAGCGGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGaa                                                       <  3:5544/139‑1 (MQ=255)

                                                                                     cGACACCCGCCAACAGCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGaa                                                       <  1:100150/139‑1 (MQ=255)

                                                                                     cGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCGGCATGTGTCAGTGGTTTTCACCGTCATCACCGAAACGCGCGAGACGaa                                                       <  5:3424/139‑1 (MQ=255)

                                                                                     cGACACCCGCCAACACCCGCCGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGaa                                                       <  2:180776/139‑1 (MQ=255)

                                                                                       ACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGCGGGTTTGACCGGCATCACCGACACGCGCGAGACGAAAg                                                     <  6:287973‑M1/139‑1 (MQ=255)

                                                                                        CACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAgg                                                    >  8:28701‑M1/1‑139 (MQ=255)

                                                                                          CCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGc                                                  >  4:229973‑M1/1‑139 (MQ=255)

                                                                                             gCCAACACCCGCTGACGCGGCCGGTCGGGCTTGTCTGCTCCCGGGATCCGCTTAAAGACAAGCTGTGCCCGTCTCCGGGGGCTGGATGTGTACGAGGTTTTGACCGTCATACCCGAAAAGCGCGAGACGAAAGGGCCTc                                               >  6:228479/1‑139 (MQ=255)

                                                                                                 acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                                           <  8:102320/139‑1 (MQ=255)

                                                                                                     ccGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCCCCGAAACGCGCGAGACGAAAGGGCCTCGTGATACg                                       <  3:137113/139‑1 (MQ=255)

                                                                                                     ccGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACg                                       <  3:94862/139‑1 (MQ=255)

                                                                                                     ccGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACg                                       <  2:128193/139‑1 (MQ=255)

                                                                                                       gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                                      >  3:35963/1‑58 (MQ=255)

                                                                                                           aCGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAGAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTa                                   <  5:199054/137‑1 (MQ=255)

                                                                                                             gcgcCCTGACGGGCTTGTCTGCTACCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTAtttt                               >  7:86028/1‑139 (MQ=255)

                                                                                                               gcCGTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTAACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  2:158444/139‑1 (MQ=255)

                                                                                                               gcCCTGACTGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  3:241611/139‑1 (MQ=255)

                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  1:236285/139‑1 (MQ=255)

                                                                                                               gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  8:224056/139‑1 (MQ=255)

                                                                                                                   tGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTa                                   >  1:61736/1‑129 (MQ=255)

                                                                                                                   tGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAgg                         >  5:259727/1‑139 (MQ=255)

                                                                                                                     aCGGGCTTGTCTGCTCGCGGCATGCGCTTACAGACAAGATGTGACCGTCTCCGGGAGCTGCTTGTGTCAGAGGTTTTGACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGtt                       <  2:55133/139‑1 (MQ=255)

                                                                                                                          cTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                  <  6:249841/139‑1 (MQ=255)

                                                                                                                          cTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCAGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                  >  4:249783/1‑139 (MQ=255)

                                                                                                                           ttGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                  >  3:116932/1‑138 (MQ=255)

                                                                                                                           ttGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                  >  2:247259/1‑138 (MQ=255)

                                                                                                                             gTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  6:179480/139‑1 (MQ=255)

                                                                                                                             gTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  2:272733/139‑1 (MQ=255)

                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  7:168654/138‑1 (MQ=255)

                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  7:14687/138‑1 (MQ=255)

                                                                                                                              tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCGCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  2:101630/138‑1 (MQ=255)

                                                                                                                                  cTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                                                    >  7:250353/1‑49 (MQ=255)

                                                                                                                                  cTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGataa          >  8:42096/1‑139 (MQ=255)

                                                                                                                                   tCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACg                                       <  6:65274/109‑1 (MQ=255)

                                                                                                                                   tCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGataa          >  5:12911/1‑138 (MQ=255)

                                                                                                                                   tCCCGGCATCCGCTTACAGACAAGCGGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  6:46905/119‑1 (MQ=255)

                                                                                                                                       ggCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGACACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATg     >  5:77703/1‑139 (MQ=255)

                                                                                                                                       ggCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGCTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATg     >  1:43435/1‑139 (MQ=255)

                                                                                                                                        gCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATgg    <  8:182752/139‑1 (MQ=255)

CCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTT  >  Exported/6139‑6415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`