| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | BS168_reference_genome | 2,581,732 | (T)6→7 | intergenic (‑115/+39) | pstS ← / ← pbpA | phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,581,726 | 1 | . | T | 92.9% | 326.6 / 15.4 | 99 | intergenic (‑109/+45) | pstS/pbpA | phosphate ABC transporter (phosphate binding lipoprotein)/transpeptidase (penicillin‑binding protein 2A) |
| Reads supporting (aligned to +/- strand): ref base . (1/6); new base T (67/24); total (69/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.07e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 101 total aligned reads displayed.
CATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑G‑TTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCC > BS168_reference_genome/2581591‑2581849 | cATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑G‑ttt > 4:186032/1‑139 (MQ=255) tAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑G‑tttt‑t > 5:291594/1‑139 (MQ=255) aTTTGTTTTTTTTCAAGTCCTGAATTCCCCCTGCGTATATTGTGTAATTAGTCCTACACGTAAAGAGAAAAGGTTCTATGTAAAAAGGGTTTGATCCGAATCTAAAGGTTGTGTAAAT‑AAGTCC‑GATTGT‑TTGTAT‑aa > 6:247144/1‑138 (MQ=255) tttttttAATTTCCTGAATTCCCCCTGCGTATAATGTTTAATTTGACATACACGTATAGAGTAACGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑aaaaaa‑aa < 7:136547/138‑1 (MQ=255) tttCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGcc > 1:67404/1‑138 (MQ=255) tttCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGcc < 3:186032/138‑1 (MQ=255) aTTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGAAT‑AAAAAA‑AACGCCCACATCATGTGAg < 5:387997/138‑1 (MQ=255) ccTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGAGAGAG‑‑ttttt > 1:193988/1‑139 (MQ=255) gCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑tttttttt > 7:477975/1‑139 (MQ=255) gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCag > 1:185495/1‑138 (MQ=255) gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCag > 8:429432/1‑138 (MQ=255) gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCag > 4:336272/1‑138 (MQ=255) gtAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCag > 2:275650/1‑138 (MQ=255) tAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACTCACACATCATGTGAGC‑‑‑TTTTTTTTTAGTTATCag > 7:259684/1‑136 (MQ=255) tAATTGGTCAAACACTTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGACCCGAATGTTAAGGTTTTGTAAAT‑CAGGGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCaga < 5:158899/138‑1 (MQ=255) aTTTTTCATACAGGTTTAGATTATAGGTTCTTTTTAAAATGGTTTTTAACCTAAGGTTATGGTTTTTTAAAT‑CAGTTCTT‑TTTT‑TTTTAA‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCagaag < 8:161754/138‑1 (MQ=255) tttGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGGGCG‑‑GTTTTTTTTATTTTTGAGAATAc > 3:573257/1‑139 (MQ=255) tCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTtg > 3:446867/1‑139 (MQ=255) ccTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTtgt > 8:399006/1‑139 (MQ=255) ccTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTtgt > 7:36819/1‑139 (MQ=255) cacGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGCCGTTGTGttt > 4:3778/1‑139 (MQ=255) cacGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACCTTGTGttt > 7:270616/1‑139 (MQ=255) ataGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTTTCAGAAGACGTTGTGTTTTCTg > 1:196423/1‑138 (MQ=255) ataGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTg > 3:333090/1‑138 (MQ=255) agagTAATGGTTCTATTTAAAAAGGGTTTGAACCGAATGTTATGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTTTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCa < 8:477975/138‑1 (MQ=255) agTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCAttt > 4:198911/1‑139 (MQ=255) gTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTTGTTATCAGAAGACGTTGTGTTTTCTGCAtttt > 4:437494/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCT‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTTCATTttc > 5:337718/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCATAAGACGTTTTGTTTTCTTCATTttc > 8:222274/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc > 1:266239/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc > 6:283534/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc > 6:257700/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc > 2:496687/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc > 5:518402/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc > 5:194962/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttc > 4:283463/1‑139 (MQ=255) tAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑‑TTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct > 2:69150/1‑139 (MQ=255) atggTTCTATGTAAACCGGGTTTGAACCTAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt < 6:291594/137‑1 (MQ=255) aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt > 4:245058/1‑139 (MQ=255) aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct > 8:53098/1‑138 (MQ=255) aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct > 7:221236/1‑138 (MQ=255) aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct > 3:462573/1‑138 (MQ=255) aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttct > 4:179843/1‑138 (MQ=255) aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TGTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCAttttagt > 4:75770/1‑136 (MQ=255) aaGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TGTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt > 1:537054/1‑139 (MQ=255) aGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTttctt > 8:165784/1‑138 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 3:522673/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 5:568645/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 3:105219/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 5:444334/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 4:196446/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 5:362042/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 3:112739/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 8:108499/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 5:199432/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 1:13419/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAttt > 5:142759/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATAATAATACGTTGTGTTTTATTCATTTTATTCAAttt > 4:52552/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑‑TTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAAtttt > 2:33269/1‑139 (MQ=255) cTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑G‑TTTTATTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TATATTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTACTTCAAttt > 5:157353/1‑139 (MQ=255) aTGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg > 5:219854/1‑139 (MQ=255) aTGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCACACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTg > 3:368454/1‑139 (MQ=255) aaaacGTGTTTGAACATAATGTTAAGTTTTTGTAACT‑CCGTGC‑GTTTTT‑TTTTAA‑AAAAAA‑CACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCtt < 3:75770/139‑1 (MQ=255) aaaaGGTTTTGAACATAATGTTAAGTTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑‑TTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTa < 8:259684/139‑1 (MQ=255) aaaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCttt > 8:111095/1‑139 (MQ=255) aaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTTGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCtttt > 3:384258/1‑138 (MQ=255) aaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTa > 8:558232/1‑139 (MQ=255) aaaGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTa > 2:225177/1‑139 (MQ=255) gTTTGAACCGAATGGTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCTGGTGTGCG‑‑TGTTTTGGTTGTTTTCAGTAGACGGTGTGTGTTCTGCTTTTTGTTGAATTTTGGCTTTATTcgt > 8:508745/1‑137 (MQ=255) ttGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACTTTGTGTTTTCTGCATTTTCTTCAATTTTGTCGTTATTCtttt > 4:347582/1‑139 (MQ=255) ttGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCtttt > 7:406367/1‑139 (MQ=255) ttGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTATTTATTCtttt > 1:529446/1‑139 (MQ=255) ttGAACCGAATGTTAAGGTTTTGTAAAT‑AAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGAGT‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTAAATTTTGTCTTTATTCttt > 8:371437/1‑139 (MQ=255) aaCCTAATTTTCAGGTTTTTTAAAT‑CCGGGC‑T‑TTTT‑TTTTAA‑AAAAAACAACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCt < 3:52552/139‑1 (MQ=255) aaCCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCt < 6:568645/139‑1 (MQ=255) aaCCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAA‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGc < 8:406367/138‑1 (MQ=255) aaCCGAATGTTAAGGTTTTGTAAAT‑CAGGCCGG‑TTTT‑TTGAAA‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCt < 5:121644/139‑1 (MQ=255) aCCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTg < 6:219854/139‑1 (MQ=255) aCCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTg < 7:429432/139‑1 (MQ=255) cctattGTTACTGTTTTGTAAAT‑CAGTGG‑GTTTTT‑TTGTAT‑AAAAAA‑AAAGCCCACATCATGTGAGCGTTTTTTTTTTTAGTTATAAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCt < 5:274012/134‑1 (MQ=255) ctaaTTTTACGGTTTTTTAAATCCTGTGG‑T‑TTTT‑TTTTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAg < 1:418147/137‑1 (MQ=255) cgatttttACGGTTTTGTAAAT‑CAGGGC‑GTTTTT‑TTGTAA‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAg < 4:462573/133‑1 (MQ=255) cGATTGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAg < 3:217970/139‑1 (MQ=255) cGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAg < 6:142759/139‑1 (MQ=255) gAATGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAg > 7:200771/1‑138 (MQ=255) aaTGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTGTTTTTTCTTTTGCTGAGTa > 7:277336/1‑139 (MQ=255) aaTGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTa > 6:273541/1‑139 (MQ=255) aTGTTAAGGTTTTTTAAAT‑CTGTGCTT‑TTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc < 8:277336/139‑1 (MQ=255) aTGTTAAGGTTTTTTAAAT‑CAGGGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc < 8:385007/139‑1 (MQ=255) aTGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc < 5:273541/139‑1 (MQ=255) aTGTTAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAA‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑‑TTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc < 1:69150/138‑1 (MQ=255) aTGTAAAGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTTTAA‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc < 6:194962/139‑1 (MQ=255) ttttAAGGTTTTTTAAAT‑CAGTGG‑GTTTTT‑TTTTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc < 4:198089/136‑1 (MQ=255) ggTTATGGTTTTGAAAAT‑CGGTGG‑G‑TTTT‑TTTGATAAAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc < 2:196423/137‑1 (MQ=255) atgggtttGTAAAA‑CAGGGC‑GTTTTT‑TTGAAA‑AAAAAA‑CACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACcttct < 4:446867/134‑1 (MQ=255) aaGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc > 5:542809/1‑134 (MQ=255) aaGGTTTTGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTAc < 6:542809/134‑1 (MQ=255) ttGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTcc > 3:198089/1‑139 (MQ=255) ttGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTc < 2:67404/138‑1 (MQ=255) ttGTAAAT‑CAGTGC‑GTTTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTATTTGTcc > 5:151581/1‑139 (MQ=255) | CATAAGCACCAATTTGTTTTTTTTCATTTCCTGAATTCCCCCTGCGTATAATGTGTAATTTGTCCTACACGTATAGAGTAAAGGTTCTATGTAAAAAGGGTTTGAACCGAATGTTAAGGTTTTGTAAAT‑CAGTGC‑G‑TTTT‑TTGTAT‑AAAAAA‑AACGCCCACATCATGTGAGCG‑‑TTTTTTTTTAGTTATCAGAAGACGTTGTGTTTTCTGCATTTTCTTCAATTTTGTCTTTATTCTTTTGCTGAGTACCTTCTTTGTCC > BS168_reference_genome/2581591‑2581849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |