BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	166,037	+T	intergenic (+4/‑27)	trnI‑Gly → / → trnI‑Arg	tRNA‑Gly/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,037	1	.	T	95.8%	315.5 / 7.6	119	intergenic (+4/‑27)	trnI‑Gly/trnI‑Arg	tRNA‑Gly/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base . (5/0); new base T (48/66); total (53/66)
Fisher's exact test for biased strand distribution p-value = 1.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

GCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCG  >  BS168_reference_genome/165900‑166169

gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTat                                                                                                                                      <  2:416011/139‑2 (MQ=255)

gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTat                                                                                                                                      <  1:334388/139‑2 (MQ=255)

gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGAGATATGCGGAAGTAGTTCAGTGGTATAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTat                                                                                                                                      <  3:9168/139‑2 (MQ=255)

   tAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTatcc                                                                                                                                    >  1:338398/1‑135 (MQ=255)

    aaaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCGTCCGC‑TGC‑A‑TTTatact                                                                                                                                   >  5:124097/1‑134 (MQ=255)

     aaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTaa                                                                                                                                 <  1:548806/139‑1 (MQ=255)

        gTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACgg                                                                                                                              >  4:135681/1‑139 (MQ=255)

         tattatTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACggg                                                                                                                             <  5:444311/139‑1 (MQ=255)

         tattatTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACggg                                                                                                                             <  3:400341/139‑1 (MQ=255)

         tattatTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACggg                                                                                                                             <  5:74911/139‑1 (MQ=255)

           ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCATGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGC‑GGC‑A‑TTTATCATAACGGGa                                                                                                                            >  7:124337/1‑138 (MQ=21)

           ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCTTCCGC‑TCC‑A‑TTTATCCTAACGGGat                                                                                                                           >  3:33244/1‑139 (MQ=255)

            tattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGat                                                                                                                           <  4:143558/138‑1 (MQ=255)

            tattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGat                                                                                                                           >  3:143558/1‑138 (MQ=255)

                 ccAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGt                                                                                                                      <  1:412992/138‑1 (MQ=255)

                   aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGCATCCCGTCGTCCGCGCCC‑A‑TTAT‑CCTAACTGGATATTGTaaa                                                                                                                   >  4:410191/1‑139 (MQ=21)

                   aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTaaa                                                                                                                   <  3:28985/139‑1 (MQ=255)

                   aaGGGTTCTCATAAGGCGAAATCTTTTTTTAGTGCGATATGCGGAAGTAGTTCCGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTaaa                                                                                                                   <  5:134894/139‑1 (MQ=21)

                         tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATGCCGTCTTCCGC‑TCC‑A‑TTTATACTAACGGGATATTGTTaattgaat                                                                                                             >  7:397404/1‑139 (MQ=21)

                         tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAaattgaat                                                                                                             >  7:422348/1‑139 (MQ=255)

                         tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑ATTATACGAACGGGATATTTTAaattgaat                                                                                                             >  3:211767/1‑139 (MQ=18)

                         tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTGGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTTaattgaat                                                                                                             >  7:125349/1‑139 (MQ=21)

                           tcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAaattgaattg                                                                                                           >  6:284821/1‑139 (MQ=255)

                             aTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAaattgaattg                                                                                                           <  5:245252/137‑1 (MQ=255)

                                aGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGcc                                                                                                       <  6:359901/138‑1 (MQ=255)

                                aGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTATAAATTGAATTGCGccc                                                                                                      >  2:352032/1‑139 (MQ=255)

                                 ggAGAAATCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTATAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCg                                                                                                     <  1:313095/139‑1 (MQ=255)

                                 ggAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCg                                                                                                     <  7:145379/139‑1 (MQ=255)

                                    gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                                  <  5:577029/139‑1 (MQ=255)

                                    gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                                  <  7:29983/139‑1 (MQ=255)

                                    gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                                  <  6:187985/139‑1 (MQ=255)

                                     aaaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGCACACCACCTTGCGTAGGTGGGGGGCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGTGATATTGTATATTGATTTGCGCCCGTAg                                                                                                  <  4:455286/138‑1 (MQ=21)

                                     aaaGATTTTTTTATTGCGATATGCGGAAGAAGTTCAGTGGTACACCACCACCTTTCCAAGGTGGGTGTCGCGCTGTCGCATACCCTCTACCGC‑TAC‑A‑TATATGCTAACGGGAAATTTTAAATTAAATTTCGCCGCTAGc                                                                                                 >  6:47500/1‑139 (MQ=255)

                                         cTTTTTTTATTGCGATTTGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                             <  5:457299/139‑1 (MQ=255)

                                         cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                             <  5:301644/139‑1 (MQ=255)

                                            tttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAt                                                                                            <  1:116148/137‑1 (MQ=255)

                                                 aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                                      <  4:213944/138‑1 (MQ=255)

                                                 aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                                      <  1:155011/138‑1 (MQ=255)

                                                 aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                                      <  6:518014/138‑1 (MQ=255)

                                                    gCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTaa                                                                                                                                 >  3:490471/1‑92 (MQ=255)

                                                    gCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTaa                                                                                                                                 <  4:490471/92‑1 (MQ=255)

                                                        tatGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGttt                                                                              <  5:64248/139‑1 (MQ=255)

                                                              gAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTAc                                                                        <  3:402475/139‑1 (MQ=255)

                                                              gAAGGAGTTCAGGGGGAGACCACCACCTTGCCACGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAACTTGAATTGCGCCCGTAGCGCAATTGGATAGAGCGTTTGACTAc                                                                        <  8:318237/139‑1 (MQ=25)

                                                               aaGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACg                                                                       <  3:143009/139‑1 (MQ=255)

                                                                   agtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                                      <  4:551249/120‑1 (MQ=255)

                                                                    gtTCAGTTGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATACTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATc                                                                   >  6:246823/1‑138 (MQ=255)

                                                                    gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTTGAATACCGTCTTCCGC‑TCC‑A‑GATATAATAACGGGATATTTTAAATTTAATTTCGCCCGTCGCTCAAATTGATAGAGCGTATGTCTACggtta                                                                   >  4:115698/1‑135 (MQ=17)

                                                                    gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                                      <  8:388940/119‑1 (MQ=255)

                                                                    gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                                      >  7:388940/1‑119 (MQ=255)

                                                                    gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCTTCCGC‑GCC‑C‑TTTATCCTAACGGGATATTGTAAATTGGATTGCGCCCGTAGCTCCAATGGTTAGGGCGTGTGGCTACGGATc                                                                   >  7:542319/1‑138 (MQ=21)

                                                                     ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCAA‑TTAA‑ACTAAACGGATATTGTAAATTGAATTTCCCCCGTAGCTAAATTGGATATAGCGGTTGAATACGGATCa                                                                  >  8:328068/1‑138 (MQ=18)

                                                                     ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑C‑TTTATCCTAACGGGATATTGTTAATTGAATTGCGCCCGTAGCTCAATTGGTTAGAGCGTTTGACTACGGATCa                                                                  >  5:198055/1‑138 (MQ=25)

                                                                     ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑ATTTTT‑GCTAACGGGCTATTGTAAATTTAATTGCGCCCGTAGCTGAATTGGATAGAGCGGTTGACTGAGGATCa                                                                  >  4:499238/1‑138 (MQ=21)

                                                                     ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATGCTAACGGGATTTTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGCGCGGTTGACTACGGATCa                                                                  >  3:567932/1‑138 (MQ=25)

                                                                     ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                                 <  6:514542/139‑1 (MQ=255)

                                                                     ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                                 <  5:284821/139‑1 (MQ=255)

                                                                     ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                                 <  3:135681/139‑1 (MQ=255)

                                                                      gcAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTGCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                                 <  8:265319/137‑1 (MQ=255)

                                                                             tAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAg                                                         <  2:410515/139‑1 (MQ=255)

                                                                                 acacCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCGGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGt                                                     <  4:423538/139‑1 (MQ=255)

                                                                                    ccaccTTGCCAAGGTGGGGGTCGCGGGTTGGCATCCCGGCTTGAGC‑GCC‑ATTTTT‑ACGAACGGGATATTTTTAATTTAATTTAGCCCGGTGCGCAATTGGATATAGCGTTTGTCTACGGAGCAAAAAGTTTGGGGTTCg                                                  >  4:361301/1‑139 (MQ=16)

                                                                                    ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGGATACCCGCGTCCGC‑GAC‑A‑TTTATACTAACGGGATATTGTTAATTTAAATTCGCCCGTGGCGAAATTGGATAGATGGTTTGACTACGCGTCAAAAGGGTATGGGTTct                                                  >  6:41787/1‑138 (MQ=255)

                                                                                    ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCGTCCGC‑GCC‑A‑TTTATCCGAACGGGCTATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGCTAGAGCGTTTGACTACGGCTGAAAAGGTTAGGGGTTCg                                                  >  5:254975/1‑139 (MQ=25)

                                                                                    ccaccTTGCCAAGGTGGGGGTCGCGGGGTAGGATGACGGCTTACGG‑TCC‑A‑TATATACTAACGGGATTTTTTTAATTTATATTAGCCCGGTGCTCCATTGGGATGAGCGGTTGTGTACGGATGAAAAAGTTTTGGGttgc                                                  >  2:537390/1‑137 (MQ=255)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTTCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGGTAGGGGTTCgc                                                 >  2:335980/1‑138 (MQ=255)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                                 <  2:338398/139‑1 (MQ=255)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCA‑A‑TTTATCCTAACGGGATATTTTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                                 <  8:7605/139‑1 (MQ=255)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                                 <  5:424742/139‑1 (MQ=255)

                                                                                     caccTTGCCAAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGTTATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                                 <  1:411615/139‑1 (MQ=255)

                                                                                     caccTTGCCAAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                                 <  8:415808/139‑1 (MQ=255)

                                                                                       ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATGAAAAGGTTAGGGGTTCGAcg                                               >  8:70771/1‑138 (MQ=255)

                                                                                       ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGAct                                               <  4:33244/139‑1 (MQ=255)

                                                                                       ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCGTCCGC‑GCC‑A‑TTTATCCTAACGGGATATTGTTAATTGAATTGCGCCCGGAGCTCAATTGGAGAGAGCGGTTGACGACGGATCAAAAGGTTAGGGGTTCGAct                                               >  4:180979/1‑139 (MQ=25)

                                                                                          tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                                 <  8:260522/134‑1 (MQ=255)

                                                                                          tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcct                                            >  1:163040/1‑139 (MQ=255)

                                                                                          tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcct                                            >  7:278686/1‑139 (MQ=255)

                                                                                          tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCTTCCGC‑TCC‑A‑TTTATACTTACGGGTTATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcct                                            >  4:145539/1‑139 (MQ=25)

                                                                                                ggggggggTCGCGGGTTCGAATCCCGGCGTCCGC‑TCC‑A‑TATATCGTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGCTAGAGCGTTTGACTACGGATCAAAAGGTAAGGGGTTCGACTCCTCTCggg                                      <  2:320036/136‑1 (MQ=21)

                                                                                                ggTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCggg                                      <  2:43431/139‑1 (MQ=255)

                                                                                                ggTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCggg                                      <  3:410191/139‑1 (MQ=255)

                                                                                                ggTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTAATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCggg                                      <  7:539082/139‑1 (MQ=255)

                                                                                                 gtgtGGGTCGCGGGTTCGATTCACGTGTTCCGA‑TCC‑A‑TTTATCCTAACGTGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGATCTTTTGACTACGGATCAAAAGGTTAGTGTGTCGACTCCTCTCggg                                      <  6:331221/138‑1 (MQ=18)

                                                                                                     gggTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTTAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTGGCCTCCTCTCGGgcgcgc                                 >  8:52755/1‑139 (MQ=25)

                                                                                                     gggTCGCGGGGTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGTTATTGTTAATTGAATTTCGCCCGTAGCTCAATTGGATAGTGCGTTTGTCTACGGATGAAAAGGGTTGGGGTTGGGCTCCTCGCGGgcgccc                                 >  6:419036/1‑137 (MQ=21)

                                                                                                       gTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCa                               <  1:317703/139‑1 (MQ=255)

                                                                                                        tCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                                 >  7:256557/1‑104 (MQ=255)

                                                                                                        tCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                                 <  8:256557/104‑1 (MQ=255)

                                                                                                         cgcgGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                                >  6:163163/1‑72 (MQ=255)

                                                                                                         cgcgGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                                <  5:163163/72‑1 (MQ=255)

                                                                                                         cgcgGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCata                             <  2:155798/139‑1 (MQ=255)

                                                                                                           cgGGTTCGAATCCCGTCTTGCGC‑GCC‑A‑TTTATACTAAAGGGATATTTTAAATTTAATTTCGCCCGTAGCTCAATTGGTTAGAGCGTTTGACTACGGATAAAAAGGTTAGGGGTTGGCCTCCTCGAGGGCGCGCCctata                           >  4:380586/1‑138 (MQ=17)

                                                                                                                       ccGTCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaa                <  8:278686/138‑1 (MQ=255)

                                                                                                                          tCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaa                >  1:228841/1‑135 (MQ=255)

                                                                                                                          tCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaa                >  3:346190/1‑135 (MQ=255)

                                                                                                                          tCTTCCGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCAGATCTTTTaatagaa                >  1:424716/1‑135 (MQ=255)

                                                                                                                              ccGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAatagat           <  1:555284/136‑1 (MQ=255)

                                                                                                                               cGC‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAgg        >  4:104175/1‑138 (MQ=255)

                                                                                                                                 c‑TCC‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGaaa     >  5:82323/1‑139 (MQ=255)

                                                                                                                                     c‑A‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCg  >  1:485651/1‑139 (MQ=255)

GCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCC‑A‑TTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCG  >  BS168_reference_genome/165900‑166169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`