BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,770,869	C→T	R174C (CGT→TGT)	rsiV →	anti‑sigma(V) factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,770,869	0	C	T	99.2%	477.6 / NA	134	R174C (CGT→TGT)	rsiV	anti‑sigma(V) factor
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (83/50); minor base G (1/0); total (84/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

GTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCCGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATCCAAAT  >  BS168_reference_genome/2770739‑2771003

gTCCCCATCCAAAAACAAAAAAGGGCAGCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTCCTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTCCACCCACAGCTGTTatat                                                                                                                                 >  8:561173/1‑138 (MQ=255)

gTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTatat                                                                                                                                 >  3:501285/1‑138 (MQ=255)

gTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTatat                                                                                                                                 >  1:323827/1‑138 (MQ=255)

gTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTatat                                                                                                                                 >  7:30042/1‑138 (MQ=255)

gTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTatat                                                                                                                                 >  4:269957/1‑138 (MQ=255)

gTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTatat                                                                                                                                 >  6:567982/1‑138 (MQ=255)

gTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTatat                                                                                                                                 >  2:15504/1‑138 (MQ=255)

gTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTatat                                                                                                                                 >  8:422648/1‑138 (MQ=255)

gTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTatat                                                                                                                                 >  8:391261/1‑138 (MQ=255)

 tCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTa                                                                                                                               >  8:495745/1‑139 (MQ=255)

           aaaacaaaaaAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACGAAGCTGTTATATTACAATTGACa                                                                                                                      >  3:475417/1‑138 (MQ=255)

           aaaacaaaaaAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACa                                                                                                                      >  8:61550/1‑138 (MQ=255)

           aaaacaaaaaAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACa                                                                                                                      >  2:308514/1‑138 (MQ=255)

           aaaacaaaaaAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATATACTCTCAATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATAGAct                                                                                                                      >  3:443744/1‑137 (MQ=255)

            aaacaaaaaAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACaaa                                                                                                                    <  2:384635/139‑1 (MQ=255)

               caaaaaTGGGCATCTCATCATGTACAGTGATTACTAGACGGGCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAg                                                                                                                 <  1:455871/139‑1 (MQ=255)

                  aaaaGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAAt                                                                                                              >  1:512176/1‑139 (MQ=255)

                   aaaGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATg                                                                                                             <  4:501285/139‑1 (MQ=255)

                   aaaGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATg                                                                                                             <  1:308514/139‑1 (MQ=255)

                        gCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGCCACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATAtt                                                                                                        <  2:253959/139‑1 (MQ=255)

                               aGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTttactta                                                                                                 <  7:422648/139‑1 (MQ=255)

                               aGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTttac                                                                                                    <  7:399700/136‑1 (MQ=255)

                                 cATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACa                                                                                               >  8:509816/1‑139 (MQ=255)

                                  aTCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACAt                                                                                              >  3:193944/1‑139 (MQ=255)

                                    cTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACAtt                                                                                             >  5:140310/1‑138 (MQ=255)

                                      aCAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTaa                                                                                           >  5:399451/1‑138 (MQ=255)

                                       cAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTaa                                                                                           <  7:509816/137‑1 (MQ=255)

                                          tGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGc                                                                                      <  8:404946/139‑1 (MQ=255)

                                          tGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGc                                                                                      <  8:30042/139‑1 (MQ=255)

                                          tGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGc                                                                                      <  4:294118/139‑1 (MQ=255)

                                          tGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGc                                                                                      <  4:279385/139‑1 (MQ=255)

                                          tGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCAACTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGc                                                                                      <  7:128470/139‑1 (MQ=255)

                                            aTTACGCGACGGTCACAGACACGCCAGAGTTACTCTCGATCCGGCGCAATATTGAAACCCCACAAGCAGCTTCCTACACACAAAGCGGTTATATTACAATTGCCAAAAAGAATGATATTTTAATTACATTAAAAAGCt                                                                                     >  6:434102/1‑138 (MQ=255)

                                            aTTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCt                                                                                     >  4:154783/1‑138 (MQ=255)

                                            aTTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCt                                                                                     >  3:381691/1‑138 (MQ=255)

                                            aTTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCt                                                                                     >  5:48177/1‑138 (MQ=255)

                                               aCGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATg                                                                                                             >  7:68798/1‑111 (MQ=255)

                                               aCGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTAtt                                                                                 >  8:27093/1‑139 (MQ=255)

                                                 gagaCGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCa                                                                               <  4:203448/139‑1 (MQ=255)

                                                      ggTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCCTCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGAt                                                                          >  5:22991/1‑139 (MQ=255)

                                                      ggTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTCCACACACAGCTGTTATATTACAATTGCCAAAAAGAATGATATTTTACTTACATTAAAATGCTTATTCAAAGAt                                                                          >  8:13846/1‑139 (MQ=255)

                                                      ggTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGAt                                                                          >  3:102622/1‑139 (MQ=255)

                                                      ggTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGAt                                                                          >  1:481894/1‑139 (MQ=255)

                                                      ggTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGAt                                                                          <  7:391261/139‑1 (MQ=255)

                                                      ggTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGAt                                                                          <  7:495745/139‑1 (MQ=255)

                                                      ggTCACAGACACACCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGAt                                                                          >  4:435374/1‑139 (MQ=255)

                                                       gTCACAGTCACGCCAGATTTACTCTCTATCCGGCGCAATATTGTAACAAGACAATCTTGTTCCTACACACTAATGTGTTATATTACAATTGACATAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATg                                                                         <  2:266226/139‑1 (MQ=255)

                                                          acaGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAg                                                                       <  6:381285/138‑1 (MQ=255)

                                                           caGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAgcg                                                                     <  6:343133/139‑1 (MQ=255)

                                                           caGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAgcg                                                                     <  1:168330/139‑1 (MQ=255)

                                                           caGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAgcg                                                                     <  5:1800/139‑1 (MQ=255)

                                                                acGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGAt                                                                          <  3:36652/129‑1 (MQ=255)

                                                                acGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGAt                                                                          >  4:36652/1‑129 (MQ=255)

                                                                  gCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTatat                                                               >  5:334326/1‑138 (MQ=255)

                                                                  gCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTatat                                                               >  3:285168/1‑138 (MQ=255)

                                                                  gCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTatat                                                               >  2:145597/1‑138 (MQ=255)

                                                                  gCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTatat                                                               >  7:227903/1‑138 (MQ=255)

                                                                  gCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTatat                                                               >  4:352269/1‑138 (MQ=255)

                                                                  gCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTatat                                                               >  6:137955/1‑138 (MQ=255)

                                                                           aCTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAACGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGtcat                                                      <  7:561173/138‑1 (MQ=255)

                                                                           aCTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGtcat                                                      <  6:399451/138‑1 (MQ=255)

                                                                            ctctctATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGtcatc                                                     <  3:292247/138‑1 (MQ=255)

                                                                            ctctctATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGtcat                                                      >  2:64873/1‑137 (MQ=255)

                                                                            ctctctATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGtcat                                                      >  6:338798/1‑137 (MQ=255)

                                                                              ctctATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGt                                                  >  7:365043/1‑139 (MQ=255)

                                                                                 tATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACaaa                                                                                                                    <  8:303878/70‑1 (MQ=255)

                                                                                 tATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGAt                                                                          <  2:96029/112‑1 (MQ=255)

                                                                                 tATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGtca                                                       >  6:354835/1‑131 (MQ=255)

                                                                                 tATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGt                                                  <  7:428212/136‑1 (MQ=255)

                                                                                 tATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCa                                                >  1:519089/1‑138 (MQ=255)

                                                                                       gcgcAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACAt                                          >  7:291768/1‑138 (MQ=255)

                                                                                       gcgcAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACAt                                          >  1:197988/1‑138 (MQ=255)

                                                                                        cgcAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCa                                        <  5:137955/139‑1 (MQ=255)

                                                                                        cgcAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCa                                        <  5:367028/139‑1 (MQ=255)

                                                                                         gcAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCa                                        >  2:229555/1‑138 (MQ=255)

                                                                                           aaTATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCa                                        >  6:435974/1‑136 (MQ=255)

                                                                                           aaTATTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCa                                        >  2:251009/1‑136 (MQ=255)

                                                                                             tatTGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGaa                                   >  3:379784/1‑139 (MQ=255)

                                                                                               ttGAAACAACACAAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAAc                                  <  6:140310/138‑1 (MQ=255)

                                                                                                         acaAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAg                       <  1:108401/139‑1 (MQ=255)

                                                                                                         acaAGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAg                       <  8:227903/139‑1 (MQ=255)

                                                                                                            aGCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCa                     >  5:503596/1‑138 (MQ=255)

                                                                                                             gCATCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCaaa                   <  6:503596/139‑1 (MQ=255)

                                                                                                               aTCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATg                 <  5:567982/139‑1 (MQ=255)

                                                                                                                tCTTCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCATAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATg                 >  8:441254/1‑138 (MQ=255)

                                                                                                                  ttCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATgaag              >  7:63915/1‑139 (MQ=255)

                                                                                                                  ttCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATgaag              >  8:195735/1‑139 (MQ=255)

                                                                                                                  ttCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATgaag              >  2:167919/1‑139 (MQ=255)

                                                                                                                   tCCTACACACAAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATgaag              >  4:54208/1‑138 (MQ=255)

                                                                                                                        cacacaAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATgaagg             >  6:376018/1‑134 (MQ=255)

                                                                                                                          cacaAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATcc      <  2:197988/139‑1 (MQ=255)

                                                                                                                           acaAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATcc      >  5:410485/1‑138 (MQ=255)

                                                                                                                            caAAGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATCCaa    <  7:298615/139‑1 (MQ=255)

                                                                                                                             aaaGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAg                       >  2:469778/1‑119 (MQ=255)

                                                                                                                             aaaGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATCCaaa   >  7:473331/1‑139 (MQ=255)

                                                                                                                             aaaGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATCCaaa   >  4:143965/1‑139 (MQ=255)

                                                                                                                              aaGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATCCaaa   >  5:30460/1‑138 (MQ=255)

                                                                                                                               aGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATCCatat  >  8:47572/1‑138 (MQ=255)

                                                                                                                               aGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATCCaaat  >  2:376334/1‑138 (MQ=255)

                                                                                                                               aGCTGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATCCaaat  >  5:551468/1‑138 (MQ=255)

GTCCACATCCAAAAACAAAAAAGGGCATCTCAGCATCTACAGTGATTACGAGACGGTCACAGACACGCCAGATTTACTCTCTATCCGGCGCAATATTGAAACAACACAAGCATCTTCCTACACACAAAGCCGTTATATTACAATTGACAAAAAGAATGATATTTTACTTACATTAAAAAGCTTATTCAAAGATGAGCGCTATATCAAAGTCATCAGTCAAAACATCAAAGAACAAATGAAACAGCAAATGAAGGAAGATCCAAAT  >  BS168_reference_genome/2770739‑2771003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`