Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 376,018 Δ1 bp 100% intergenic (‑220/‑14) hxlA ← / → hxlR 3‑hexulose‑6‑phosphate synthase (HPS)/positive regulator of hxlAB expression (formaldehyde sensing)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome376,0180G.99.7% 1449.8 / ‑1.5 308intergenic (‑220/‑14)hxlA/hxlR3‑hexulose‑6‑phosphate synthase (HPS)/positive regulator of hxlAB expression (formaldehyde sensing)
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (149/158);  total (149/159)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 324 total aligned reads displayed.

TTTGAAGTCAACAAGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAGGGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTCAATGAATTAAA  >  BS168_reference_genome/375869‑376162
                                                                                                                                                     |                                                                                                                                                
  tGAAGTCAACAAGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggg                                                                                                                                                >  2:1836053/1‑150 (MQ=255)
     aGTCAACAAGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggggg                                                                                                                                             >  2:3480889/1‑150 (MQ=255)
     aGTCAACAAGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggggg                                                                                                                                             >  1:1313938/1‑150 (MQ=255)
       tCAACAAGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                           <  1:691667/150‑2 (MQ=255)
         aacaaGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggggggatt                                                                                                                                         >  1:3319904/1‑147 (MQ=255)
         aacaaGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggggggatt                                                                                                                                         >  1:1230045/1‑147 (MQ=255)
            aaGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTa                                                                                                                                     >  1:169348/1‑150 (MQ=255)
            aaGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTa                                                                                                                                     >  2:4832840/1‑150 (MQ=255)
            aaGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTa                                                                                                                                     <  1:820762/150‑1 (MQ=255)
             aGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTAt                                                                                                                                    >  2:4170585/1‑150 (MQ=255)
              gTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTAtt                                                                                                                                   <  1:2497380/150‑1 (MQ=255)
              gTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTAtt                                                                                                                                   <  1:880778/150‑1 (MQ=255)
               ttACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTTTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTg                                                                                                                                  <  1:1502925/150‑1 (MQ=255)
               ttACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTg                                                                                                                                  <  2:1634709/150‑1 (MQ=255)
                tACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGa                                                                                                                                 >  2:1874676/1‑150 (MQ=255)
                tACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGa                                                                                                                                 >  2:1227723/1‑150 (MQ=255)
                 aCTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAg                                                                                                                                <  2:2028860/150‑1 (MQ=255)
                  cTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGTGGGGATTCTATTGTGc                                                                                                                               >  2:2271457/1‑150 (MQ=255)
                    tGGAGGATACTGTGAGGAGAGATAATCAGATTTCTTACGTTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCg                                                                                                                             <  1:1978792/150‑1 (MQ=255)
                    tGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCg                                                                                                                             >  1:3420647/1‑150 (MQ=255)
                        ggaTACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATg                                                                                                                         >  2:4053896/1‑150 (MQ=255)
                        ggaTACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATg                                                                                                                         <  2:4675065/150‑1 (MQ=255)
                           tACTGTGAGGAGAGACAATCAGATTTTTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgac                                                                                                                      <  1:1009414/150‑1 (MQ=255)
                           tACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgac                                                                                                                      >  2:4341082/1‑150 (MQ=255)
                            aCTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgacg                                                                                                                     <  2:3038365/150‑1 (MQ=255)
                            aCTGTGAGGAGAGACAATCAGATTTCTTTCCCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgacg                                                                                                                     <  1:255660/150‑1 (MQ=255)
                              tgtgAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATCCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgacgac                                                                                                                   <  1:4859257/150‑1 (MQ=255)
                                   ggagAGACAATCAGATTTCTTTCCCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAg                                                                                                              <  2:4657132/150‑1 (MQ=255)
                                      gagaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGtt                                                                                                           >  2:2596690/1‑150 (MQ=255)
                                              cAGATTTCTTTCTCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCAGATGGACGACAAAAGGTTTAATtatg                                                                                                   >  2:3305676/1‑150 (MQ=255)
                                              cAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATtgtg                                                                                                   >  1:2208906/1‑150 (MQ=255)
                                                 aTTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                                <  1:3825458/150‑1 (MQ=255)
                                                 aTTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGAGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                                >  1:2321106/1‑150 (MQ=255)
                                                    tctttcGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAgg                                                                                             <  2:4742887/150‑1 (MQ=255)
                                                     ctttcGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAGAGGTTTAATTGTGAGAAGGa                                                                                            <  1:4551314/150‑1 (MQ=255)
                                                     ctttcGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGa                                                                                            >  1:2642850/1‑150 (MQ=255)
                                                      tttcGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGaa                                                                                           >  2:4114229/1‑150 (MQ=255)
                                                       ttcGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAAt                                                                                          <  1:3705419/150‑1 (MQ=255)
                                                         cGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTa                                                                                        <  2:633083/150‑1 (MQ=255)
                                                           cTTGATTTTATGAGTGAGTATAATGAAAAAANAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAAc                                                                                      <  1:1605063/150‑1 (MQ=255)
                                                            ttGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACg                                                                                     >  2:2792756/1‑150 (MQ=255)
                                                               aTTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCtt                                                                                  <  1:1794008/150‑1 (MQ=255)
                                                                ttttATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTg                                                                                 <  1:3623407/150‑1 (MQ=255)
                                                                     tgagtgagTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTg                                                                            >  2:1500205/1‑150 (MQ=255)
                                                                     tgagtgagTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTg                                                                            >  2:2472899/1‑150 (MQ=255)
                                                                       agtgagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGAt                                                                          <  1:4341082/150‑1 (MQ=255)
                                                                          gagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACAAACAAAAAGTTTACTAA‑GGGTAGGATTCTATTGAGCCGGATGTTCGACAAAAGGTTTAATTGTGAGAAAGAATTAACGCTTGCAGAGATTgg                                                                       >  1:2083435/1‑150 (MQ=255)
                                                                            gtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCg                                                                     >  1:732007/1‑150 (MQ=255)
                                                                            gtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCg                                                                     <  2:2720251/150‑1 (MQ=255)
                                                                              ataATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGt                                                                   >  2:2999909/1‑150 (MQ=255)
                                                                              ataATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGt                                                                   >  2:3670202/1‑150 (MQ=255)
                                                                                aaTGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGGTGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGGCGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTaa                                                                 >  2:1579519/1‑150 (MQ=255)
                                                                                  tGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAAt                                                               <  2:3029062/150‑1 (MQ=255)
                                                                                   gAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATg                                                              >  1:4980677/1‑150 (MQ=255)
                                                                                    aaaaaaGAGTCTGCAAGAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGAGATGCTATTGTGCCGGATGGACGGCAAAAGATTTAATTGTGAGAAGGAATTAACGCTTGCAGTGAGTGACGGTAAATgg                                                             >  2:2907360/1‑150 (MQ=255)
                                                                                      aaaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGaa                                                           >  2:822711/1‑150 (MQ=255)
                                                                                      aaaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGaa                                                           >  1:4201007/1‑150 (MQ=255)
                                                                                       aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGaaa                                                          >  2:3914017/1‑150 (MQ=255)
                                                                                       aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGaaa                                                          >  1:2806447/1‑150 (MQ=255)
                                                                                        aaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGaaaa                                                         >  2:136871/1‑150 (MQ=255)
                                                                                         agagTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAAt                                                        >  2:194844/1‑150 (MQ=255)
                                                                                          gagTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATg                                                       <  1:1090499/150‑1 (MQ=255)
                                                                                          gagTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATg                                                       <  2:1772605/150‑1 (MQ=255)
                                                                                            gTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCt                                                     <  1:445676/150‑1 (MQ=255)
                                                                                            gTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCt                                                     >  1:3030091/1‑150 (MQ=255)
                                                                                              cTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCa                                                   >  2:2617931/1‑150 (MQ=255)
                                                                                                gCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCAtt                                                 >  1:4575112/1‑150 (MQ=255)
                                                                                                 cAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCAttt                                                >  1:3222176/1‑150 (MQ=255)
                                                                                                 cAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCAttt                                                >  2:3779091/1‑150 (MQ=255)
                                                                                                 cAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCAttt                                                >  2:3873084/1‑150 (MQ=255)
                                                                                                 cAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCAttt                                                >  2:113770/1‑150 (MQ=255)
                                                                                                   aaaaaGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCAtttt                                               <  2:2050441/149‑1 (MQ=255)
                                                                                                     aaaGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATg                                            <  2:1548140/150‑1 (MQ=255)
                                                                                                        gtaagtaagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCa                                         >  1:3562528/1‑150 (MQ=255)
                                                                                                         taagtaagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCAt                                        >  2:941197/1‑150 (MQ=255)
                                                                                                         taagtaagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCAt                                        <  1:1874678/150‑1 (MQ=255)
                                                                                                          aagtaagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCAtt                                       <  2:207894/150‑1 (MQ=255)
                                                                                                            gtaagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTa                                     <  1:3879936/150‑1 (MQ=255)
                                                                                                             taagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAg                                    <  2:2418024/150‑1 (MQ=255)
                                                                                                             taagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAg                                    <  1:1919912/150‑1 (MQ=255)
                                                                                                             taagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAg                                    >  1:842061/1‑150 (MQ=255)
                                                                                                              aagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAgg                                   >  2:3521250/1‑150 (MQ=255)
                                                                                                                gtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGaa                                 >  2:394820/1‑150 (MQ=255)
                                                                                                                  gatgatACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGaaaa                               <  2:732007/150‑1 (MQ=255)
                                                                                                                    tgatACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAaaga                             >  2:4576883/1‑150 (MQ=255)
                                                                                                                      atACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAaagaag                           <  2:3155130/150‑1 (MQ=255)
                                                                                                                        aCGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGc                         <  2:2214750/150‑1 (MQ=255)
                                                                                                                        aCGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGc                         <  2:3420647/150‑1 (MQ=255)
                                                                                                                             tCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACaaa                    <  2:4999385/150‑1 (MQ=255)
                                                                                                                              cTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACaaaa                   <  2:541388/150‑1 (MQ=255)
                                                                                                                              cTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACaaaa                   >  2:806567/1‑150 (MQ=255)
                                                                                                                              cTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCAATTTATGGCATATAGGAAAAGAAGGCACaaaa                   <  1:3164451/150‑1 (MQ=255)
                                                                                                                               tAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAAc                  <  2:4273010/150‑1 (MQ=255)
                                                                                                                               tAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAAc                  <  1:4027607/150‑1 (MQ=255)
                                                                                                                                aaCATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACg                 >  1:3070543/1‑150 (MQ=255)
                                                                                                                                  cATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTGACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGt               <  1:2860891/150‑1 (MQ=255)
                                                                                                                                   aTACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAAGAAAAGAAGGCACAAAACGGtt              >  2:3216383/1‑150 (MQ=255)
                                                                                                                                     aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTCa            <  1:4642314/150‑1 (MQ=255)
                                                                                                                                        aaaaGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGTCATTTAGGAAAAGAAGGCACAAAACGGTTCAATg         >  1:43958/1‑150 (MQ=255)
                                                                                                                                        aaaaGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTCAATg         <  2:3651843/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TTTGAAGTCAACAAGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAGGGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTCAATGAATTAAA  >  BS168_reference_genome/375869‑376162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑