BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	1,317,152:2	+T	90.6%	intergenic (+23/+5)	phrA → / ← yjpA	secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,317,152	2	.	T	90.6%	768.9 / 71.0	245	intergenic (+23/+5)	phrA/yjpA	secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
Reads supporting (aligned to +/- strand): ref base . (9/14); new base T (108/111); total (119/126)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

Only 100 of 256 total aligned reads displayed.

TAAATGGATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑C‑T‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCGT  >  BS168_reference_genome/1317003‑1317287

 aaaTGGATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑gg‑gg                                                                                                                                             >  2:4793627/1‑149 (MQ=255)

  aaTGGATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑gg‑ggt                                                                                                                                            >  2:3056336/1‑148 (MQ=255)

  aaTGGATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑gg‑ggt                                                                                                                                            >  1:1847362/1‑148 (MQ=255)

   aTGGATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑gg‑ggtc                                                                                                                                           <  2:4177936/150‑4 (MQ=255)

    tGGATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑gg‑ggtct                                                                                                                                          >  1:403598/1‑146 (MQ=255)

    tGGATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑gg‑ggtct                                                                                                                                          >  1:2761831/1‑146 (MQ=255)

      gATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑G‑t‑c‑ttt                                                                                                                                   >  1:4883644/1‑150 (MQ=255)

       aTGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑G‑t‑c‑tttt                                                                                                                                  <  1:1549964/150‑2 (MQ=255)

       aTGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑G‑t‑c‑tttt                                                                                                                                  >  2:4479232/1‑149 (MQ=255)

         gTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATGTCATATTGGGGCACGCAATCAAACATGATGCATAAATAAAGACCCTTA‑GG‑G‑‑G‑T‑C‑tttttt                                                                                                                                <  1:1985519/150‑1 (MQ=255)

          tCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑G‑T‑C‑tttttta                                                                                                                               >  2:2545872/1‑149 (MQ=255)

           cAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑G‑T‑C‑ttttttat                                                                                                                              <  1:2232776/150‑3 (MQ=255)

             ggTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑G‑T‑C‑ttttttattt                                                                                                                            <  2:3907056/150‑5 (MQ=255)

             ggTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑G‑T‑C‑ttttttattt                                                                                                                            >  1:2858345/1‑146 (MQ=255)

              gTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑G‑T‑C‑ttttttatttc                                                                                                                           <  1:724519/150‑6 (MQ=255)

               tttGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑G‑T‑C‑ttttttatttct                                                                                                                          <  2:2298827/150‑7 (MQ=255)

                ttgttgCTCGTTGCGGTCGGGTTCAGCTTTACTTAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATttctt                                                                                                                            <  2:3926500/150‑1 (MQ=255)

                  gttgCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GGTGT‑CTT‑T‑TTTATTTCTTCa                                                                                                                          >  1:4707908/1‑150 (MQ=255)

                  gttgCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCa                                                                                                                          <  1:1762539/150‑1 (MQ=255)

                  gttgCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCa                                                                                                                          >  2:1850641/1‑150 (MQ=255)

                   ttgCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAg                                                                                                                         <  1:3785414/150‑1 (MQ=255)

                   ttgCTCGTTGCGGTCGGGTACAGCTTTACAGAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAg                                                                                                                         <  1:312184/150‑1 (MQ=255)

                         gTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCa                                                                                                                   <  1:257254/150‑1 (MQ=255)

                         gTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCa                                                                                                                   >  2:1050570/1‑150 (MQ=255)

                          ttGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAATCACAGAAGGGAAAACATTTCATATTCCGGCACGCAATCAATCATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTTCAt                                                                                                                  <  2:2432677/150‑1 (MQ=255)

                          ttGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCAt                                                                                                                  <  2:2862381/150‑1 (MQ=255)

                            gCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTACGG‑GT‑CTT‑T‑TTTATTTCTTCAGCTTCCATTc                                                                                                                <  1:3857100/150‑1 (MQ=255)

                              ggTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCtt                                                                                                              >  1:2868442/1‑150 (MQ=255)

                               gTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCttt                                                                                                             >  1:3066385/1‑150 (MQ=255)

                               gTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCttt                                                                                                             <  1:3132743/150‑1 (MQ=255)

                               gTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCttt                                                                                                             >  2:2078626/1‑150 (MQ=255)

                                 cGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTa                                                                                                           >  2:3791244/1‑150 (MQ=255)

                                 cGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTa                                                                                                           <  2:2197840/150‑1 (MQ=255)

                                  gggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGTCCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGGTTCCATTCTTTTAt                                                                                                          >  1:2323357/1‑150 (MQ=255)

                                   ggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATc                                                                                                         <  1:3661439/150‑1 (MQ=255)

                                   ggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATc                                                                                                         <  1:4114365/150‑1 (MQ=255)

                                        aGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGAAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAg                                                                                                    <  1:2643957/150‑1 (MQ=255)

                                         gCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGc                                                                                                   <  1:1693650/150‑1 (MQ=255)

                                           tttACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTc                                                                                                 >  2:3681482/1‑150 (MQ=255)

                                            ttACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAAACAAACATGATGCATAAAAGAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCa                                                                                                <  1:1487401/150‑1 (MQ=255)

                                              aCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCaga                                                                                              >  1:4096033/1‑150 (MQ=255)

                                                 cAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCagaaga                                                                                           >  1:4981985/1‑150 (MQ=255)

                                                  aGGTGATGCTTCATGTAGGGGAAACAGCAAACACAGAAGGGAAAACATTTCACATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGAt                                                                                          <  2:141230/150‑1 (MQ=255)

                                                    gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATcc                                                                                        <  1:1896331/150‑1 (MQ=255)

                                                       aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACt                                                                                     >  1:4868405/1‑150 (MQ=255)

                                                       aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACt                                                                                     >  1:4400156/1‑150 (MQ=255)

                                                       aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACACTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACt                                                                                     <  1:857385/150‑1 (MQ=255)

                                                         ggTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTC‑TCG‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTg                                                                                   >  1:1048934/1‑150 (MQ=255)

                                                         ggTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCAGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GGAGTAC‑T‑T‑TTTATTTCTTCAGATTCCATTCTTTTATCGTCAGCTCAGAAGGTCCCCTTg                                                                                   >  1:480126/1‑150 (MQ=255)

                                                           ttCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGcc                                                                                 <  2:485332/150‑1 (MQ=255)

                                                                gCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAg                                                                            <  2:1632898/150‑1 (MQ=255)

                                                                 cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGc                                                                           <  1:4512444/150‑1 (MQ=255)

                                                                 cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGc                                                                           >  1:2993437/1‑150 (MQ=255)

                                                                 cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGc                                                                           >  2:4874168/1‑150 (MQ=255)

                                                                  aGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCg                                                                          >  2:2706480/1‑150 (MQ=255)

                                                                   ggTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGTCACGCAACCAATCATGATGCATAAAATAATACCCGTA‑GG‑GGCCTT‑T‑TTTATTCCTTAAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTTCCACCAGCgg                                                                         <  2:1459241/150‑1 (MQ=255)

                                                                     tGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGAt                                                                       >  1:877094/1‑150 (MQ=255)

                                                                      gAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATc                                                                      <  1:1252162/150‑1 (MQ=255)

                                                                      gAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATc                                                                      >  2:2914703/1‑150 (MQ=255)

                                                                       aaaCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATcc                                                                     <  1:2530560/150‑1 (MQ=255)

                                                                       aaaCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATcc                                                                     <  1:4216148/150‑1 (MQ=255)

                                                                          cAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCa                                                                  >  1:3269301/1‑150 (MQ=255)

                                                                              aaaCACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGc                                                              >  2:3962028/1‑150 (MQ=255)

                                                                               aaCACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGcc                                                             <  1:1438684/150‑1 (MQ=255)

                                                                                 cacaGAAGGGAAAACTTTTCATATTGCGGCACGCAATCAAACATGATGCATAATAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGa                                                           <  1:3341337/150‑1 (MQ=255)

                                                                                  acaGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGAt                                                          <  2:261287/150‑1 (MQ=255)

                                                                                   caGAAGGGAAAACATTTCATATTACGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTT‑GG‑GGTCTT‑T‑TTTATTTCTTCAACTTCCATTCTTTTATCGTCAGGTCAGAAGATCCACTTCCCACCAGCGGATCCGCAGGGCCGAtt                                                         >  1:2125391/1‑150 (MQ=255)

                                                                                     gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTc                                                       <  2:4577877/150‑1 (MQ=255)

                                                                                     gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GATCTT‑T‑TTTATTTCTTGAGCTTCCATTCTTTTATCGTCAGCTCTAAAGATCCACTTGCCACCAACGGATCCGCAAGGCCGATTTc                                                       >  1:261487/1‑150 (MQ=255)

                                                                                       aGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                                     >  2:551962/1‑150 (MQ=255)

                                                                                       aGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTC‑T‑TATTTATTTCTTCAGCTTCCATTCTTTTATCCTGAGGTCAGAAGATACACTTGCCACCAGCGGATCCCCATGGCCGNTTTCCg                                                     >  1:2823877/1‑150 (MQ=255)

                                                                                           aaaaCATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACTCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTTCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGc                                                 <  2:1910378/150‑1 (MQ=255)

                                                                                            aaccATTTCATATTGCGGCACGCAATCAAACATGATGAATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGcc                                                <  2:2006325/147‑1 (MQ=255)

                                                                                             aaCATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCct                                               <  2:3075024/150‑1 (MQ=255)

                                                                                                aTTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCtt                                            >  1:3603670/1‑150 (MQ=255)

                                                                                                    cATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAg                                        <  2:4110915/150‑1 (MQ=255)

                                                                                                      tatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTg                                      >  1:907504/1‑150 (MQ=255)

                                                                                                       atTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGa                                     <  2:2761831/150‑1 (MQ=255)

                                                                                                          gCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATc                                  >  2:2989110/1‑150 (MQ=255)

                                                                                                           cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCTGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCt                                 >  1:620053/1‑150 (MQ=255)

                                                                                                           cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCt                                 <  1:2498862/150‑1 (MQ=255)

                                                                                                            ggCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTg                                >  2:1948829/1‑150 (MQ=255)

                                                                                                            ggCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTg                                <  2:710073/150‑1 (MQ=255)

                                                                                                             gCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGc                               <  2:4096033/150‑1 (MQ=255)

                                                                                                             ccccGCAACCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGc                               <  2:1244680/147‑1 (MQ=255)

                                                                                                              cACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCt                              <  1:1860537/150‑1 (MQ=255)

                                                                                                                      cAAACATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGAc                      >  2:4361782/1‑150 (MQ=255)

                                                                                                                       aaaCATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACa                     >  1:3447486/1‑150 (MQ=255)

                                                                                                                          catcatGCATAAAAAAACACCCTTA‑GG‑GATCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAACGGATCCGCATGGCCGATTTTCGCTGCCTCTTCCAGTGAAACTGATTCGATGACATAc                  >  1:629310/1‑150 (MQ=255)

                                                                                                                          cATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATAc                  <  2:3269301/150‑1 (MQ=255)

                                                                                                                          cATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATAc                  >  2:4559672/1‑150 (MQ=255)

                                                                                                                          cATGATGCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATAc                  <  1:4559672/150‑1 (MQ=255)

                                                                                                                            tgatgCATAAAAAAATACCCTTA‑GG‑GAACTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGACAGCTCAGCAGATTCACTTGCATCCAGCGGATCCGCATGCTCGATTTCCGCTGGCTCTTCCAGTGAATCTGCTTCGATGACATACGc                >  1:1303079/1‑150 (MQ=255)

                                                                                                                                gCATAAAAAAAGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACTTACGCTccc            >  1:1062982/1‑149 (MQ=255)

                                                                                                                                     aaaaaaGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCtgt       >  1:792560/1‑150 (MQ=255)

                                                                                                                                     aaaaaaGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCtgt       >  2:939051/1‑150 (MQ=255)

                                                                                                                                     aaaaaaGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCACTGAATCTGCTTCGATGACATACGATCCGCCtgt       >  2:3425643/1‑150 (MQ=255)

                                                                                                                                       aaaaGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTgg     <  2:877094/150‑1 (MQ=255)

                                                                                                                                        aaaGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGc    >  1:2380907/1‑150 (MQ=255)

                                                                                                                                          aGACCCTTA‑GG‑GGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCGt  >  1:1428058/1‑150 (MQ=255)

TAAATGGATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTA‑GG‑G‑‑C‑T‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCGT  >  BS168_reference_genome/1317003‑1317287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`