BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	1,317,153:1	+T	100%	intergenic (+24/+4)	phrA → / ← yjpA	secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,317,153	1	.	T	91.2%	789.8 / 63.9	239	intergenic (+24/+4)	phrA/yjpA	secreted inhibitor of the activity of phosphatase RapA (quorum sensing)/putative enzyme
Reads supporting (aligned to +/- strand): ref base . (10/11); new base T (105/113); total (115/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 245 total aligned reads displayed.

GATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑C‑T‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCGT  >  BS168_reference_genome/1317009‑1317287

  tGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑G‑t‑c‑ttttt                                                                                                                                 >  2:1151039/1‑148 (MQ=255)

  tGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑G‑t‑c‑ttttt                                                                                                                                 <  1:2179588/150‑3 (MQ=255)

   gTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATGTCATATTGGGGCACGCAATCAAACATGATGCATAAATAAAGACCCTTAGGG‑‑G‑T‑C‑tttttt                                                                                                                                <  1:1985519/150‑1 (MQ=255)

    tCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑G‑T‑C‑tttttta                                                                                                                               >  2:2545872/1‑149 (MQ=255)

     cAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑G‑T‑C‑ttttttat                                                                                                                              <  1:2232776/150‑3 (MQ=255)

      aGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑G‑T‑C‑ttttttatt                                                                                                                             >  2:2194702/1‑147 (MQ=255)

       ggTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑G‑T‑C‑ttttttattt                                                                                                                            >  1:2858345/1‑146 (MQ=255)

        gTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑G‑T‑C‑ttttttatttc                                                                                                                           <  1:724519/150‑6 (MQ=255)

         tttGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑G‑T‑C‑ttttttatttct                                                                                                                          <  2:577979/150‑7 (MQ=255)

            gttgCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCa                                                                                                                          >  2:1850643/1‑150 (MQ=255)

            gttgCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCa                                                                                                                          <  1:1762539/150‑1 (MQ=255)

             ttgCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGCCCCTTAGGGGTCTT‑T‑TTTATTTCTTCAg                                                                                                                         <  1:3785416/150‑1 (MQ=255)

             ttgCTCGTTGCGGTCGGGTACAGCTTTACAGAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAg                                                                                                                         <  1:312184/150‑1 (MQ=255)

                  cGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTcc                                                                                                                    <  1:3561662/150‑1 (MQ=255)

                   gTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCa                                                                                                                   <  1:257254/150‑1 (MQ=255)

                    ttGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCAt                                                                                                                  <  2:2862381/150‑1 (MQ=255)

                       cGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCt                                                                                                               <  2:337614/150‑1 (MQ=255)

                        ggTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCtt                                                                                                              >  2:1940811/1‑150 (MQ=255)

                        ggTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCtt                                                                                                              >  1:2868442/1‑150 (MQ=255)

                         gTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCttt                                                                                                             <  1:3132743/150‑1 (MQ=255)

                         gTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCttt                                                                                                             >  1:3066385/1‑150 (MQ=255)

                           cGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTa                                                                                                           <  2:244939/150‑1 (MQ=255)

                            gggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTAt                                                                                                          <  2:1949851/150‑1 (MQ=255)

                             ggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATc                                                                                                         <  1:4114365/150‑1 (MQ=255)

                             ggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATc                                                                                                         >  1:4336942/1‑150 (MQ=255)

                             ggTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATc                                                                                                         <  2:465625/150‑1 (MQ=255)

                               ttCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGt                                                                                                       <  2:3509314/150‑1 (MQ=255)

                                   gCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGc                                                                                                   <  1:1693650/150‑1 (MQ=255)

                                      ttACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAAACAAACATGATGCATAAAAGAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCa                                                                                                <  1:1487401/150‑1 (MQ=255)

                                        aCTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCaga                                                                                              >  1:4096033/1‑150 (MQ=255)

                                            aGGTGATGCTTCATGTAGGGGAAACAGCAAACACAGAAGGGAAAACATTTCACATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGAt                                                                                          <  2:141230/150‑1 (MQ=255)

                                              gTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATcc                                                                                        <  2:374750/150‑1 (MQ=255)

                                               tGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCa                                                                                       <  1:3376857/150‑1 (MQ=255)

                                                gATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCAc                                                                                      <  1:1610995/150‑1 (MQ=255)

                                                gATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTC‑TCT‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCAc                                                                                      >  1:1907394/1‑150 (MQ=255)

                                                gATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTC‑T‑TGTTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATacac                                                                                      >  1:88782/1‑150 (MQ=255)

                                                 aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACt                                                                                     <  2:2342116/150‑1 (MQ=255)

                                                 aTGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACACTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACt                                                                                     <  1:857385/150‑1 (MQ=255)

                                                   ggTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTC‑TCG‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTg                                                                                   >  1:1048934/1‑150 (MQ=255)

                                                      tCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGcca                                                                                >  1:2784281/1‑150 (MQ=255)

                                                       cATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGccac                                                                               >  1:4904748/1‑150 (MQ=255)

                                                        aTGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGccacc                                                                              >  1:4872049/1‑150 (MQ=255)

                                                         tGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGccacca                                                                             >  2:893378/1‑150 (MQ=255)

                                                         tGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGccacca                                                                             >  1:4517548/1‑150 (MQ=255)

                                                          gCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAg                                                                            <  2:1632898/150‑1 (MQ=255)

                                                           cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGc                                                                           >  2:4874168/1‑150 (MQ=255)

                                                           cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGc                                                                           <  1:504545/150‑1 (MQ=255)

                                                           cAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGc                                                                           >  1:2993437/1‑150 (MQ=255)

                                                           cAGGTAAAACAGAAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGc                                                                           <  1:3209811/150‑1 (MQ=255)

                                                            aGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCg                                                                          >  2:2706480/1‑150 (MQ=255)

                                                             ggTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGTCACGCAACCAATCATGATGCATAAAATAATACCCGTAGGGGCCTT‑T‑TTTATTCCTTAAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTTCCACCAGCgg                                                                         <  2:1459241/150‑1 (MQ=255)

                                                             ggTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCgg                                                                         <  1:2370421/150‑1 (MQ=255)

                                                                gAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATc                                                                      >  2:2914703/1‑150 (MQ=255)

                                                                gAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATc                                                                      <  1:1252162/150‑1 (MQ=255)

                                                                 aaaCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATcc                                                                     <  1:4216148/150‑1 (MQ=255)

                                                                 aaaCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATcc                                                                     <  1:2530560/150‑1 (MQ=255)

                                                                  aaCAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCg                                                                    <  2:411588/150‑1 (MQ=255)

                                                                           cacaGAAGGGAAAACTTTTCATATTGCGGCACGCAATCAAACATGATGCATAATAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGa                                                           <  1:3341337/150‑1 (MQ=255)

                                                                               gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTc                                                       <  2:4510440/150‑1 (MQ=255)

                                                                               gAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGATCTT‑T‑TTTATTTCTTGAGCTTCCATTCTTTTATCGTCAGCTCTAAAGATCCACTTGCCACCAACGGATCCGCAAGGCCGATTTc                                                       >  1:261487/1‑150 (MQ=255)

                                                                               gAAGGGAAAACATTTCATATTGCAGCACGCAATCAAACATAATGTATAAAAAATAACCCTTAGGGGTCTT‑T‑TTTATTGCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTc                                                       <  1:1398320/150‑1 (MQ=255)

                                                                                aaGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCTATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGACGTTTTcc                                                      >  1:360716/1‑150 (MQ=255)

                                                                                 aGGGAAAACATTTCATATTGCGGCACGCAATTAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                                     >  1:3938941/1‑150 (MQ=255)

                                                                                 aGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                                     >  1:1477829/1‑150 (MQ=255)

                                                                                 aGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCg                                                     <  2:3101532/150‑1 (MQ=255)

                                                                                 aGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTC‑T‑TATTTATTTCTTCAGCTTCCATTCTTTTATCCTGAGGTCAGAAGATACACTTGCCACCAGCGGATCCCCATGGCCGNTTTCCg                                                     >  1:2823877/1‑150 (MQ=255)

                                                                                   ggAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCt                                                   <  1:4667760/150‑1 (MQ=255)

                                                                                    gAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTg                                                  <  2:2670260/150‑1 (MQ=255)

                                                                                     aaaaCATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGc                                                 <  1:4471037/150‑1 (MQ=255)

                                                                                         cATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCctct                                             <  2:4099626/150‑1 (MQ=255)

                                                                                               atatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATTCGCATGGCCAATTTCCGCTGCCTCTTCCAGt                                       >  1:642580/1‑150 (MQ=255)

                                                                                                tatTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTg                                      >  1:907504/1‑150 (MQ=255)

                                                                                                tatTGCGGCACGCAATCAAACATGATGCAT‑AAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGa                                     <  2:2040375/150‑1 (MQ=255)

                                                                                                   tGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAAt                                   <  2:3913459/150‑1 (MQ=255)

                                                                                                    gCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATc                                  >  2:2989110/1‑150 (MQ=255)

                                                                                                     cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCTGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCt                                 >  1:620053/1‑150 (MQ=255)

                                                                                                     cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCt                                 <  2:2773980/150‑1 (MQ=255)

                                                                                                     cGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCt                                 <  1:2498862/150‑1 (MQ=255)

                                                                                                      ggCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTg                                <  2:710073/150‑1 (MQ=255)

                                                                                                       ccccGCAACCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGc                               <  2:1244680/147‑1 (MQ=255)

                                                                                                        cACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCt                              <  2:2262798/150‑1 (MQ=255)

                                                                                                        cACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCt                              <  1:1860537/150‑1 (MQ=255)

                                                                                                             aaTCAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGAt                         >  2:4568279/1‑150 (MQ=255)

                                                                                                                cAAACATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGAc                      >  2:4361782/1‑150 (MQ=255)

                                                                                                                   aCATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATa                   <  2:4868405/150‑1 (MQ=255)

                                                                                                                   aCATGATGCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATa                   <  2:3949822/150‑1 (MQ=255)

                                                                                                                     atgatgCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACg                 >  2:4030652/1‑150 (MQ=255)

                                                                                                                      tgatgCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGc                >  2:3847289/1‑150 (MQ=255)

                                                                                                                       gatgCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCt               >  1:825483/1‑150 (MQ=255)

                                                                                                                       gatgCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGATCAGAAGATCCACTTGCCACCAGCGGATCCACATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGGTTCGATGACATACGCt               >  1:3753247/1‑150 (MQ=255)

                                                                                                                          gCATAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACTTACGCTccc            >  1:1062982/1‑149 (MQ=255)

                                                                                                                            aTAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGcc          <  1:1850641/150‑1 (MQ=255)

                                                                                                                             tAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCt         >  2:447549/1‑150 (MQ=255)

                                                                                                                             tAAAAAAAGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCt         >  1:4842439/1‑150 (MQ=255)

                                                                                                                               aaaaaaGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCtgt       <  2:3066385/150‑1 (MQ=255)

                                                                                                                               aaaaaaGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCtgt       >  2:939051/1‑150 (MQ=255)

                                                                                                                                  aaaGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGc    >  1:4738017/1‑150 (MQ=255)

                                                                                                                                  aaaGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGc    >  1:2380907/1‑150 (MQ=255)

                                                                                                                                   aaGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCg   >  1:2643685/1‑150 (MQ=255)

                                                                                                                                    aGACCCTTAGGGGTCTT‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCGt  >  1:1428058/1‑150 (MQ=255)

GATGTCAGGTTTGTTGCTCGTTGCGGTCGGGTTCAGCTTTACTCAGGTGATGGTTCATGCAGGTGAAACAGCAAACACAGAAGGGAAAACATTTCATATTGCGGCACGCAATCAAACATGATGCATAAAAAAAGACCCTTAGGG‑‑C‑T‑T‑TTTATTTCTTCAGCTTCCATTCTTTTATCGTCAGCTCAGAAGATCCACTTGCCACCAGCGGATCCGCATGGCCGATTTCCGCTGCCTCTTCCAGTGAATCTGCTTCGATGACATACGCTCCGCCTGTGGCGT  >  BS168_reference_genome/1317009‑1317287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`