BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	166,037:1	+T	100%	intergenic (+4/‑27)	trnI‑Gly → / → trnI‑Arg	tRNA‑Gly/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,037	1	.	T	100.0%	722.7 / NA	206	intergenic (+4/‑27)	trnI‑Gly/trnI‑Arg	tRNA‑Gly/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (92/114); total (92/114)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

CCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGC  >  BS168_reference_genome/165890‑166178

ccGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTatc                                                                                                                                              >  2:2536440/1‑148 (MQ=255)

 cGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTatcc                                                                                                                                             <  2:291316/150‑4 (MQ=255)

  gCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTatcct                                                                                                                                            <  2:1445571/150‑5 (MQ=255)

  gCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTatcct                                                                                                                                            <  2:1843116/150‑5 (MQ=255)

   cTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTatccta                                                                                                                                           <  2:1107953/150‑6 (MQ=255)

    ttGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTCTTTTATTGCGATATGCGGGAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCTCTCCATTTATCCTaa                                                                                                                                          >  2:1385284/1‑150 (MQ=25)

     tGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAAc                                                                                                                                         >  2:4135326/1‑150 (MQ=255)

        aaGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACggg                                                                                                                                      <  1:189519/150‑1 (MQ=255)

         aGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGa                                                                                                                                     <  1:4611728/150‑1 (MQ=255)

         aGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGa                                                                                                                                     >  1:3949940/1‑150 (MQ=255)

         aGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGa                                                                                                                                     <  2:3949940/150‑1 (MQ=255)

            taaaaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGatat                                                                                                                                  <  1:475153/148‑1 (MQ=255)

                aTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTa                                                                                                                              <  1:4597948/150‑1 (MQ=255)

                    attattACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAaatt                                                                                                                          >  1:2799592/1‑150 (MQ=255)

                         taCCAAGGTTTCTCATAAGGAGATAGCTTTTTTTATTGCGATATGGGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAATGGGATATTGTAaattgaatt                                                                                                                     <  1:1048610/150‑1 (MQ=25)

                         taCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAaattgaatt                                                                                                                     <  1:3254692/150‑1 (MQ=255)

                          aCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAaattgaattg                                                                                                                    >  2:532416/1‑150 (MQ=255)

                           ccAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTgc                                                                                                                   <  1:1007050/150‑1 (MQ=255)

                           ccAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTgc                                                                                                                   >  2:2494485/1‑150 (MQ=255)

                            cAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTgcg                                                                                                                  <  1:2328540/150‑1 (MQ=255)

                            cAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTgcg                                                                                                                  <  1:2848853/150‑1 (MQ=255)

                            cAAGGTTTCTCATAAGGAGAAAGCTTGTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAAGTGGGGGTCGGGGGATCGTATGCCGTCTTCCGCTCCATTTATCCTAAAGGAGTATTGTATATTGGATTgcg                                                                                                                  >  2:3896217/1‑150 (MQ=21)

                              aGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGcc                                                                                                                <  2:2716901/150‑1 (MQ=255)

                                  ttCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTa                                                                                                            <  1:4135326/150‑1 (MQ=255)

                                     tcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                                         <  2:1349486/150‑1 (MQ=255)

                                     tcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGGCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGAAAATTGAATTGCGCCCGTAGCt                                                                                                         >  1:3162147/1‑150 (MQ=255)

                                      cttAAGGAGAATTCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTc                                                                                                        <  1:2196174/148‑1 (MQ=255)

                                      cATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTc                                                                                                        >  1:4915522/1‑150 (MQ=255)

                                       aTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCa                                                                                                       >  1:1592960/1‑150 (MQ=255)

                                       aTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCACGTAGCTCa                                                                                                       >  2:4355062/1‑150 (MQ=255)

                                         aaGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAt                                                                                                     <  2:4004370/150‑1 (MQ=255)

                                          aGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAtt                                                                                                    <  1:2996358/150‑1 (MQ=255)

                                          aGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAtt                                                                                                    >  1:3133711/1‑150 (MQ=255)

                                          aGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAtt                                                                                                    >  1:3873408/1‑150 (MQ=255)

                                            gagaAAGCTTTTTTTATTGCGATATGTGGAAGTAGTTCAGTGGGAGAACACCACCTTGCCAATGCGGGGACCGCGGGTTCGCATCCCGCCTTCCGCTCCATTTATCCTAACGCGGTATTGTAGATTGAATTGCGCCCGCAGCTCAATTgg                                                                                                  >  1:262674/1‑150 (MQ=255)

                                             agaAAGCTTTTTTTATTGCGATATGCGGGAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCAATTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGa                                                                                                 >  2:1697327/1‑150 (MQ=255)

                                                       ttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGttt                                                                                       <  1:3089166/150‑1 (MQ=255)

                                                       ttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGCGTTCGAATCCCGTCTTTCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGttt                                                                                       <  1:2296270/150‑1 (MQ=255)

                                                         ttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGa                                                                                     <  1:2692138/150‑1 (MQ=255)

                                                         ttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGa                                                                                     <  2:4154366/150‑1 (MQ=255)

                                                           aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCTATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACt                                                                                   >  1:3992359/1‑150 (MQ=255)

                                                           aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACt                                                                                   <  2:2955640/150‑1 (MQ=255)

                                                           aTTGCGATATGCGGAAGCAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCTCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGAGATATTGTTAATTGAATTGCGCCCGTAGCTCAATTGGATAGAACGTTTGACt                                                                                   >  2:4977229/1‑150 (MQ=25)

                                                            ttGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTa                                                                                  >  2:2156582/1‑150 (MQ=255)

                                                                  tatGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGTCTACGGATc                                                                            >  1:3459310/1‑150 (MQ=255)

                                                                    tGCGGAAGTAGTTCAGTGGTAGAACACCACCNTGCCAAGGAGTAGGTCTCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTTCGGATCaa                                                                          <  1:1648149/150‑1 (MQ=25)

                                                                      cGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaaaa                                                                        >  1:1709225/1‑150 (MQ=255)

                                                                      cGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaaaa                                                                        <  1:3609642/150‑1 (MQ=255)

                                                                        gAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAgg                                                                      <  1:4789797/150‑1 (MQ=255)

                                                                           gtagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTCACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTa                                                                   <  1:1386113/150‑1 (MQ=255)

                                                                           gtagtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTa                                                                   <  2:1684376/150‑1 (MQ=255)

                                                                             agtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAgg                                                                 <  1:1192359/150‑1 (MQ=255)

                                                                             agtTCAGTGGTACAACCCCCCCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAgg                                                                 <  1:2898567/150‑1 (MQ=37)

                                                                                tCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGt                                                              <  1:748577/150‑1 (MQ=255)

                                                                                tCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGt                                                              <  2:3210851/150‑1 (MQ=255)

                                                                                 cAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGtt                                                             <  1:3698530/150‑1 (MQ=255)

                                                                                   gTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCg                                                           <  2:4656919/150‑1 (MQ=255)

                                                                                        aGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGGAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcc                                                      >  1:2104673/1‑150 (MQ=255)

                                                                                           acacCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCctct                                                   <  1:709355/150‑1 (MQ=255)

                                                                                            caccacCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCtctc                                                  <  2:4267017/150‑1 (MQ=255)

                                                                                             accaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGCCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGCTTAGGGGTTCGACTCCTCTCg                                                 <  1:2911050/150‑1 (MQ=255)

                                                                                              ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCgg                                                <  1:4182273/150‑1 (MQ=255)

                                                                                              acaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCgg                                                <  1:4355062/149‑1 (MQ=255)

                                                                                               caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCggg                                               <  1:1677968/150‑1 (MQ=255)

                                                                                                accTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgc                                              >  1:3358900/1‑150 (MQ=255)

                                                                                                  cTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgcgc                                            >  2:1274843/1‑150 (MQ=255)

                                                                                                       cAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCat                                       <  2:2253213/150‑1 (MQ=255)

                                                                                                         aGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCatat                                     <  2:1076986/150‑1 (MQ=255)

                                                                                                         aGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCatat                                     >  2:3469524/1‑150 (MQ=255)

                                                                                                         aGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCatat                                     <  2:1938328/150‑1 (MQ=255)

                                                                                                         aGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCatat                                     <  1:2494485/150‑1 (MQ=255)

                                                                                                         aGGTGGGGGCCGCGGGTTCGATTCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTCCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGTTTAGGGGTTCGACTCCTCTCGGGCGCGCCatat                                     <  1:546770/150‑1 (MQ=21)

                                                                                                            tGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCtt                                  <  2:3282085/150‑1 (MQ=255)

                                                                                                            tGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCtt                                  <  1:141450/150‑1 (MQ=255)

                                                                                                               gggTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTa                               <  1:638290/150‑1 (MQ=255)

                                                                                                                   cgcgGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatag                           <  1:3365376/150‑1 (MQ=255)

                                                                                                                   cgcgGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatag                           >  1:3048160/1‑150 (MQ=255)

                                                                                                                    gcgGGTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaataga                          >  2:2026456/1‑150 (MQ=255)

                                                                                                                       ggTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaata                       <  1:2156582/150‑1 (MQ=255)

                                                                                                                       ggTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGACGCGCCATATCTTTTaatagaata                       >  1:587687/1‑150 (MQ=255)

                                                                                                                        gTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaatag                      <  2:2475560/150‑1 (MQ=255)

                                                                                                                        gTTCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaatag                      <  1:2568277/150‑1 (MQ=255)

                                                                                                                          tCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGTCTACGGATCAAAGGGGTAGAGGTTCGACTCCTCTCCGACGCGCCATATCTTCTAATAGAatagat                    >  1:839371/1‑150 (MQ=18)

                                                                                                                          tCGAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAatagat                    <  2:742331/150‑1 (MQ=255)

                                                                                                                            gAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAatagatag                  <  2:2799592/150‑1 (MQ=255)

                                                                                                                            gAATCCCGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAatagatag                  >  1:3788377/1‑150 (MQ=255)

                                                                                                                                cccGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGaaa              >  2:3174428/1‑150 (MQ=255)

                                                                                                                                cccGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGaaa              >  2:4614555/1‑150 (MQ=255)

                                                                                                                                  cGTCTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATc            >  1:1499245/1‑150 (MQ=255)

                                                                                                                                     cTTCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCggg         <  2:1473112/150‑1 (MQ=255)

                                                                                                                                      ttCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGa        <  2:1623086/150‑1 (MQ=255)

                                                                                                                                      ttCCGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGa        >  2:4518119/1‑150 (MQ=255)

                                                                                                                                        ccGCTCCATTTATCGTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGAGAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCACTCGGGCGCGCCATATCTCTTAATAGAATAGCTAGGAAATCGGGAAg      <  2:3694114/150‑1 (MQ=17)

                                                                                                                                         cGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGTGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGt     >  1:957339/1‑150 (MQ=255)

                                                                                                                                         cGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGt     <  2:48951/150‑1 (MQ=255)

                                                                                                                                         cGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGt     >  1:1825199/1‑150 (MQ=255)

                                                                                                                                         cGCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCACAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGt     <  1:2044064/150‑1 (MQ=255)

                                                                                                                                          gCTCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTa    <  2:262674/150‑1 (MQ=255)

                                                                                                                                           cTCCATTTATCCTAACGGGATATTGTAAATTGAATTGTGCCCGTAGCTCAATTGGATAGTGCGTTTGTCTACGGATCAAAAGGTTAGGGGTTCGACTTCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAg   <  1:5007549/150‑1 (MQ=17)

                                                                                                                                            tCCATTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGc  <  1:4494107/150‑1 (MQ=255)

CCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCATTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAATCGGGAAGTAGC  >  BS168_reference_genome/165890‑166178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`