BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	143,659	C→T	100%	S100F (TCT→TTT)	rpsE →	ribosomal protein S5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	143,659	0	C	T	100.0%	1285.3 / NA	351	S100F (TCT→TTT)	rpsE	ribosomal protein S5
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (184/167); total (184/167)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 351 total aligned reads displayed.

GTAAAGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTAAAC  >  BS168_reference_genome/143512‑143805

gTAAAGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTg                                                                                                                                                  <  2:1792834/150‑1 (MQ=255)

    aGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAgg                                                                                                                                              <  1:3102148/150‑1 (MQ=255)

      cacaAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTa                                                                                                                                            <  2:1500310/150‑1 (MQ=255)

        caAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACt                                                                                                                                          <  1:4317601/150‑1 (MQ=255)

        caAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACt                                                                                                                                          <  2:2571161/150‑1 (MQ=255)

          agaagTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGTACGTTTCGGTGCACGTAACATCTTGTTAAAACCTGCTTTTGAAGCTACTgc                                                                                                                                        >  1:851624/1‑149 (MQ=255)

                aCCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTAt                                                                                                                                  <  2:545879/150‑1 (MQ=255)

                  cAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCg                                                                                                                                >  2:3845108/1‑150 (MQ=255)

                     aaGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTg                                                                                                                             >  2:4352794/1‑150 (MQ=255)

                      aGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTgg                                                                                                                            >  2:2781235/1‑150 (MQ=255)

                         gATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAgg                                                                                                                         >  2:2354166/1‑150 (MQ=255)

                         gATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGCAGTTATCGCTGGAgg                                                                                                                         >  1:3992182/1‑150 (MQ=255)

                            tCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGccc                                                                                                                      <  1:2720979/150‑1 (MQ=255)

                              gCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGTAGGTACTGGAGTTATCGCTGGAGGCCCTg                                                                                                                    >  2:4929420/1‑150 (MQ=255)

                              gCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTg                                                                                                                    <  2:4445479/150‑1 (MQ=255)

                              gCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTg                                                                                                                    <  1:795209/150‑1 (MQ=255)

                                  aGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACg                                                                                                                >  2:1759074/1‑150 (MQ=255)

                                        tGAAGGTGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGGCGGGTCGGGGCAGGTAACATCTTGTTAAAACCTGCTTTTGGAGGTACTGGAGGGATCGGTGGCGGCCCTGGACGTGCGGt                                                                                                          >  2:478205/1‑150 (MQ=255)

                                         gAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTa                                                                                                         >  2:3930178/1‑150 (MQ=255)

                                         gAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTa                                                                                                         <  1:601705/150‑1 (MQ=255)

                                          aaGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTAc                                                                                                        >  2:1207418/1‑150 (MQ=255)

                                          aaGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTAc                                                                                                        >  2:1642946/1‑150 (MQ=255)

                                           aGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTATAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAAGCCCTGTACGTGCGGTACt                                                                                                       >  1:499356/1‑150 (MQ=255)

                                           aGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACt                                                                                                       >  1:2221578/1‑150 (MQ=255)

                                           aGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACt                                                                                                       >  2:735325/1‑150 (MQ=255)

                                               gCGAAAAACAATGTGGTTGAAGTACCACTGGTTGTAACTACAATTCCACACGAAATCCTTGGACGTTTCGGTGCAGGTAACATCTTGCTAAAACCTCTTGTTGAATGAATTGGAGTAATCGCGGGAGGCACAGTACGTGCGGTACTTGag                                                                                                   <  2:324941/150‑1 (MQ=255)

                                                cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATCCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagc                                                                                                  <  1:99596/150‑1 (MQ=255)

                                                  aaaaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagcta                                                                                                <  2:3116216/150‑1 (MQ=255)

                                                   aaaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagctag                                                                                               <  1:2257532/150‑1 (MQ=255)

                                                    aaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGGAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagctagc                                                                                              >  1:1380008/1‑150 (MQ=255)

                                                       gAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTgg                                                                                           >  2:1088049/1‑150 (MQ=255)

                                                         aTTTGATTGAATTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGACCTGTACGTGAGGTACTTGAGCTAGCTGgtg                                                                                         <  1:2612867/150‑1 (MQ=255)

                                                          tttGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgtgt                                                                                        <  2:4225170/150‑1 (MQ=255)

                                                            tgattgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAg                                                                                      >  2:4647341/1‑150 (MQ=255)

                                                             gattgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGc                                                                                     >  1:4937402/1‑150 (MQ=255)

                                                              attgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCt                                                                                    >  2:158130/1‑150 (MQ=255)

                                                              attgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCt                                                                                    <  1:1000800/150‑1 (MQ=255)

                                                               ttgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTg                                                                                   <  2:1348218/150‑1 (MQ=255)

                                                               ttgaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTg                                                                                   <  1:1081997/150‑1 (MQ=255)

                                                                 gaAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGat                                                                                 <  2:5014342/150‑1 (MQ=255)

                                                                   aGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGatat                                                                               >  1:2156448/1‑150 (MQ=255)

                                                                   aGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGatat                                                                               <  1:3320559/150‑1 (MQ=255)

                                                                   aGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGatat                                                                               <  1:1237045/150‑1 (MQ=255)

                                                                   aGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGatat                                                                               >  1:202044/1‑150 (MQ=255)

                                                                    gTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATc                                                                              <  1:3845108/150‑1 (MQ=255)

                                                                    gTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCCGATATc                                                                              >  2:4896093/1‑150 (MQ=255)

                                                                      aCCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCt                                                                            >  2:2855845/1‑150 (MQ=255)

                                                                       ccAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCtt                                                                           <  2:2933046/150‑1 (MQ=255)

                                                                        cAATGGTTGGAACTACATTTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCttt                                                                          >  2:429878/1‑150 (MQ=255)

                                                                         aaTGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTc                                                                         >  1:1714412/1‑150 (MQ=255)

                                                                          aTGGTTGGAACTACAATTCCACACGAAATCATCGTACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCt                                                                        <  1:268090/150‑1 (MQ=255)

                                                                          aTGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCt                                                                        >  1:3018883/1‑150 (MQ=255)

                                                                          aTGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTACCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCt                                                                        >  2:844531/1‑150 (MQ=255)

                                                                           tGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTa                                                                       <  2:2313804/150‑1 (MQ=255)

                                                                            ggTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTaa                                                                      >  1:470769/1‑150 (MQ=255)

                                                                             gTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAg                                                                     <  2:1070978/150‑1 (MQ=255)

                                                                              ttGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGt                                                                    >  2:2057528/1‑150 (MQ=255)

                                                                              ctGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGGACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGt                                                                    >  2:4716789/2‑150 (MQ=255)

                                                                                ggAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCt                                                                  <  1:3564620/150‑1 (MQ=255)

                                                                                 gAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                                 <  1:375192/150‑1 (MQ=255)

                                                                                 gAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                                 <  1:1332039/150‑1 (MQ=255)

                                                                                  aaCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCttt                                                                <  2:1428199/150‑1 (MQ=255)

                                                                                      aCAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGt                                                            >  2:932087/1‑150 (MQ=255)

                                                                                        aaTTCCACACAAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTc                                                          <  1:4511501/150‑1 (MQ=255)

                                                                                         aTTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCt                                                         >  2:4548744/1‑150 (MQ=255)

                                                                                         aTTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCt                                                         <  1:4834742/150‑1 (MQ=255)

                                                                                             cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAaca                                                     <  1:4409130/150‑1 (MQ=255)

                                                                                              acacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGTAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAacac                                                    >  2:365299/1‑150 (MQ=255)

                                                                                              acacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAacac                                                    <  2:4981287/150‑1 (MQ=255)

                                                                                                  gAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCg                                                <  2:243290/150‑1 (MQ=255)

                                                                                                  gAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCg                                                >  1:1991769/1‑150 (MQ=255)

                                                                                                  gAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCg                                                >  2:1916175/1‑150 (MQ=255)

                                                                                                  gAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGNNCTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTTTAACACACCg                                                >  1:907859/1‑150 (MQ=255)

                                                                                                    aaTCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGAt                                              >  2:2529081/1‑150 (MQ=255)

                                                                                                        atcGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAAc                                          <  1:2857555/150‑1 (MQ=255)

                                                                                                        atcGGACGGTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGGGGCCCTGTACGTGCGCTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAAc                                          >  2:3551322/1‑150 (MQ=255)

                                                                                                             aCGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGAt                                     >  1:2071753/1‑150 (MQ=255)

                                                                                                              cGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGAtt                                    <  1:2434587/150‑1 (MQ=255)

                                                                                                              cGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGAtt                                    >  1:2927478/1‑150 (MQ=255)

                                                                                                              cGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGAtt                                    >  2:4504671/1‑150 (MQ=255)

                                                                                                                  tCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTg                                >  2:4130076/1‑150 (MQ=255)

                                                                                                                   cGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGc                               <  2:215516/150‑1 (MQ=255)

                                                                                                                    ggTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGNTTCTAACACACCGATCAACATGATTCGTGCa                              >  1:1635594/1‑150 (MQ=255)

                                                                                                                     gTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCaa                             >  2:560654/1‑150 (MQ=255)

                                                                                                                          ggTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACtt                        >  2:3170087/1‑150 (MQ=255)

                                                                                                                           gTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTc                       >  2:1675351/1‑150 (MQ=255)

                                                                                                                           gTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTc                       <  1:4192770/150‑1 (MQ=255)

                                                                                                                              aCATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAg                    >  1:2506141/1‑150 (MQ=255)

                                                                                                                              aCATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAg                    <  1:3470287/150‑1 (MQ=255)

                                                                                                                              aCATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAg                    <  1:4886423/150‑1 (MQ=255)

                                                                                                                                      ttAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGt            >  2:2253627/1‑150 (MQ=255)

                                                                                                                                      ttAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGt            <  1:4464222/150‑1 (MQ=255)

                                                                                                                                        aaaaCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGa          >  1:3423600/1‑150 (MQ=255)

                                                                                                                                        aaaaCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGa          <  2:245778/150‑1 (MQ=255)

                                                                                                                                        aaaaCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGa          <  1:2781235/150‑1 (MQ=255)

                                                                                                                                             cTGCTTTTGAAGGTACTGGAGTTTTCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTa     >  1:4736637/1‑150 (MQ=255)

                                                                                                                                             cTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTa     <  1:546496/150‑1 (MQ=255)

                                                                                                                                              tGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTaa    <  2:3614523/150‑1 (MQ=255)

                                                                                                                                               gCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTaaa   <  2:3326899/150‑1 (MQ=255)

                                                                                                                                                cTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTAAAc  <  1:1515527/150‑1 (MQ=255)

GTAAAGCACAAGAAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAGTGAACTTAAAC  >  BS168_reference_genome/143512‑143805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`