| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 1,349,495 | G→T | 70.8% | A325A (GCC→GCA) | pitB ← | putative low‑affinity inorganic phosphate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,349,495 | 0 | G | T | 70.8% | 420.6 / 256.1 | 240 | A325A (GCC→GCA) | pitB | putative low‑affinity inorganic phosphate transporter |
| Reads supporting (aligned to +/- strand): ref base G (46/24); new base T (92/78); total (138/102) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 242 total aligned reads displayed.
AGAATAAAACCATATTCGAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTGGGTTGTACTGACCGG > BS168_reference_genome/1349346‑1349644 | aaCCATATTCGAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATgg < 2:1093708/150‑1 (MQ=255) ttCGAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAg > 1:230973/1‑150 (MQ=255) gAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAGAATATCATATTTAAAATACAGTATGCGATGGCACCAAgagt > 2:2268705/1‑150 (MQ=255) gAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACGAAgtgt > 1:21297/1‑150 (MQ=255) gAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAgtgt > 1:4412670/1‑150 (MQ=255) aaTCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTc > 1:1016964/1‑150 (MQ=255) aaTCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTc < 2:3328552/150‑1 (MQ=255) aaTCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTc < 2:3778939/150‑1 (MQ=255) cccTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCt < 1:1924184/150‑1 (MQ=255) tCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGACACCAAGTGTCGCTGaa > 1:572075/1‑150 (MQ=255) acaTCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAAt > 2:637414/1‑150 (MQ=255) aTCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCg > 1:1184586/1‑150 (MQ=255) aTCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCg > 1:1700936/1‑150 (MQ=255) gAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAg > 2:2467261/1‑150 (MQ=255) gAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAg < 1:243925/150‑1 (MQ=255) aaTAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCg < 1:4784406/150‑1 (MQ=255) tAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtg < 1:2174404/150‑1 (MQ=255) aGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACTGCTCCTTTTACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAACGtga > 1:3405405/1‑150 (MQ=255) ggTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGtgat > 2:2099936/1‑150 (MQ=255) tCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGAc < 2:4019694/150‑1 (MQ=255) cTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGAcc > 1:3147752/1‑150 (MQ=255) tGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGAccc < 1:1989616/150‑1 (MQ=255) tcTTATTGTTTTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGAAAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCa < 1:653263/149‑1 (MQ=255) gCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAATCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCa < 1:1333339/150‑1 (MQ=255) ttATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATg < 2:2402059/150‑1 (MQ=255) tATTGTATTCGAGAGACCCGGATGGTTTTCCTGTTCCAACAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAATTATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAAGCGGAAGCGTGATGACCCATGt < 1:1884894/150‑1 (MQ=255) aTTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTa < 1:309148/150‑1 (MQ=255) aTTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTa < 1:1627536/150‑1 (MQ=255) tGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAAt > 1:1225197/1‑150 (MQ=255) gTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATg < 1:4136507/150‑1 (MQ=255) tATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGa > 1:3613208/1‑150 (MQ=255) tCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCa > 1:2364656/1‑150 (MQ=255) cGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCAt > 2:3760087/1‑150 (MQ=255) gagagaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCAtt < 2:1686072/150‑1 (MQ=255) gagaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCg < 1:3988685/150‑1 (MQ=255) gaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCt < 1:3527933/150‑1 (MQ=255) gaTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCt < 1:4892204/150‑1 (MQ=255) aTCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCtt < 2:2158749/150‑1 (MQ=255) ccGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTgg > 2:1840307/1‑150 (MQ=255) cGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGc < 2:436051/150‑1 (MQ=255) ggATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGcc < 2:4297646/150‑1 (MQ=255) tGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGTCAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTgggccta > 2:959497/1‑145 (MQ=255) gTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTAcc > 2:2987073/1‑150 (MQ=255) gTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTAcc > 2:1870643/1‑150 (MQ=255) tttCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTAcccc > 1:1742172/1‑150 (MQ=255) cTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGtt > 2:308423/1‑150 (MQ=255) cTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGtt < 2:679651/150‑1 (MQ=255) cTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGtt > 1:1525884/1‑150 (MQ=255) ttCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTAc < 2:1184586/150‑1 (MQ=255) tCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACg > 2:1437651/1‑150 (MQ=255) ccATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGc > 1:4280215/1‑150 (MQ=255) aaaaaaaCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCtttt < 2:2732025/150‑1 (MQ=255) aaaaaaCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTa > 1:4570195/1‑150 (MQ=255) aaaaaCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAc < 2:576579/150‑1 (MQ=255) aaaaCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcg > 1:2046789/1‑150 (MQ=255) aaaaCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAAAGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcg > 1:1495748/1‑150 (MQ=255) aaaaCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcg > 1:3310687/1‑150 (MQ=255) aaaCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcgc > 1:4812028/1‑150 (MQ=255) aaaCCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAcgc > 2:4077461/1‑150 (MQ=255) cGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTg < 2:847553/150‑1 (MQ=255) cGCTCCTTTGACACTAGCGGTGTGTCATTAAGATATCTTATTTGAATTATAGTATGCGTTGGCACCAACTGTCGCTGAATTCGGAAGCGTGATGACCCATGTTATGAGCATTCGCTTGGCCGTACCCCCGTTTTCGCCTTTTACGCGCTg > 1:3705032/1‑150 (MQ=255) gCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTgg > 1:3843723/1‑150 (MQ=255) gCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTgg > 1:2254324/1‑150 (MQ=255) ccTTTGACAGGAGCGGTGTGACATTAAAATATCATACTTAAAATAATGTATGCGATGTCACCAAGTGTCGCTGAAATCGCAAGCGTGGTGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTCTACGCCTTTTACGTGGTGGGAc > 2:4171712/1‑150 (MQ=255) ccTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGAc < 2:105789/150‑1 (MQ=255) ccTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGAc < 2:3942124/150‑1 (MQ=255) aaCAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATCTAATGAGCATTCGGTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCAcc < 1:1397244/149‑1 (MQ=255) aGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGAc > 1:109291/1‑150 (MQ=255) aGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGAc < 2:2886544/150‑1 (MQ=255) aGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGAc < 2:2592461/150‑1 (MQ=255) aGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGAc < 2:2083170/150‑1 (MQ=255) aaGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGc > 2:3013921/1‑150 (MQ=255) cGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCaa > 1:3546690/1‑150 (MQ=255) cGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTAAGCGGTGGGACGCACCGACGCCaa > 1:546811/1‑150 (MQ=255) gtgtGACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGa > 1:1495874/1‑150 (MQ=255) gtgtGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGa > 1:2992051/1‑150 (MQ=255) tgACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAAtt > 2:4733619/1‑150 (MQ=255) gACATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATtg < 2:4570199/150‑1 (MQ=255) cATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATtgat > 1:4314646/1‑150 (MQ=255) cATTAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATtgat > 1:4628660/1‑150 (MQ=255) cATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATtgat > 1:4198598/1‑150 (MQ=255) cATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATtgat > 2:1358294/1‑150 (MQ=255) tAAAATATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATgaa > 1:933286/1‑150 (MQ=255) aaaaTATCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATgaag > 1:804913/1‑150 (MQ=255) aaTATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATgaagaa > 1:306017/1‑150 (MQ=255) aaTATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATgaagaa > 2:388010/1‑150 (MQ=255) aaTATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATgaagaa > 2:4432377/1‑150 (MQ=255) atatCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGaaa < 2:2364656/150‑1 (MQ=255) atCATATTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATg > 2:3893751/1‑150 (MQ=255) tatTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGt < 2:360735/150‑1 (MQ=255) atTTAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCCGAAGCGTTGTGACACATGTAATGAGCATTCGCTTGGCCGTACCCCAGTATACGCCTTTTACGCGGTGAGACGCACCGACACCGAGAATTGATGAAGAAATAACGTg > 2:4997019/1‑150 (MQ=255) tttAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTgg > 1:2947032/1‑150 (MQ=255) tttAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTgg > 1:2677310/1‑150 (MQ=255) tAAAATAAAGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTGGGt < 2:729452/150‑1 (MQ=255) tAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTGGGt > 2:3486180/1‑150 (MQ=255) aaaaTAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTGGGtt < 2:4487520/150‑1 (MQ=255) aaaGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTGGGTTGTACt > 2:3284866/1‑150 (MQ=255) aGTATGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTGGGTTGTACTGa > 2:157582/1‑150 (MQ=255) gTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTGGGTTGTACTGAc > 1:4960633/1‑150 (MQ=255) tAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTGGGTTGTACTGAcc > 1:2742350/1‑150 (MQ=255) | AGAATAAAACCATATTCGAATCCCTCACATCTGAAATAGGTTCTGCTTATTGTATTCGAGAGATCCGGATGGTTTTCCTGTTCCATCAAAAAAACCGCTCCTTTGACAGAAGCGGTGTGACATTAAAATATCATATTTAAAATAAAGTAGGCGATGGCACCAAGTGTCGCTGAAATCGGAAGCGTGATGACCCATGTAATGAGCATTCGCTTGGCCGTACCCCAGTTTACGCCTTTTACGCGGTGGGACGCACCGACGCCAAGAATTGATGAAGAAATGACGTGGGTTGTACTGACCGG > BS168_reference_genome/1349346‑1349644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |