Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,463,035 G→C 5.6% G153G (GGC→GGG)  ykwB ← putative acetyltransferase / amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,463,0350GC 5.6% 807.9 / 6.3 295G153G (GGC→GGG) ykwBputative acetyltransferase / amidohydrolase
Reads supporting (aligned to +/- strand):  ref base G (126/135);  new base C (2/26);  total (128/167)
Fisher's exact test for biased strand distribution p-value = 1.17e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 317 total aligned reads displayed.

CCAAATAATTGCTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATGATTTTT  >  BS168_reference_genome/1462887‑1463184
                                                                                                                                                    |                                                                                                                                                     
ccAAATAATTGCTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGNACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGgc                                                                                                                                                      <  1:910369/150‑1 (MQ=255)
 cAAATAATTGCTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGgcc                                                                                                                                                     <  2:2786369/150‑1 (MQ=255)
   aataatTGCTGACTACACGAATAGGTGAACGCCCGCAACGGGGGAGAAAGGACAGCACAGGGTCGTTTTTCATGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGgccgc                                                                                                                                                   >  1:1905054/1‑150 (MQ=255)
    ataatTGCTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCATCCATCACATCTCCAACTTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGgccgcc                                                                                                                                                  <  1:2275738/150‑1 (MQ=255)
      aatTGCTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGgccgccgc                                                                                                                                                >  1:4501099/1‑150 (MQ=255)
       atTGCTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGgccgccgcc                                                                                                                                               >  1:3991857/1‑150 (MQ=255)
         tGCTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGccgccgccgg                                                                                                                                             >  1:3854508/1‑149 (MQ=255)
          gCTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGCACAGCACAGGGTCGTCTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGTCGCCGCCGCCgag                                                                                                                                            >  1:4156782/1‑150 (MQ=255)
          gCTGACTACACGAATAGGTGAACGCCCCCAAGGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGCCCGCCGCCgag                                                                                                                                            <  2:50239/150‑1 (MQ=255)
           cTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTGTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGGGTATTTATAATACAAAGGAATGCGGCAGCCGCCgaga                                                                                                                                           >  1:1226052/1‑150 (MQ=255)
            tGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGaa                                                                                                                                          >  1:578871/1‑150 (MQ=255)
              aCTACACGAATAGGTGAACGCCCGCACGGGAGGAGAAAGGACAGCCCAGGGTCGTTTTTCCTTCCTTCCATCACATCTTCAACCTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAgg                                                                                                                                        <  2:458308/150‑1 (MQ=255)
               cTACAAGAATATGTGAACCCCCCCAGAGGAGAAGAAAGGACAGCCCAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAATGTACTGAACAGCGCTGATGTGGTGTGTGTATTTTTAATAAAAAGGAATGGGCCCGCCGCCGAGAAGGc                                                                                                                                       <  1:1781669/150‑1 (MQ=255)
                   acGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTc                                                                                                                                   <  2:4299534/150‑1 (MQ=255)
                    cGAATAGGTGAACCCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGCCCGCCGCCGAGAAGGCGTTcc                                                                                                                                  <  1:3900623/150‑1 (MQ=255)
                     gAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCa                                                                                                                                 <  1:309295/150‑1 (MQ=255)
                     gAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGACCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCa                                                                                                                                 <  1:4188876/150‑1 (MQ=255)
                       aTAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGt                                                                                                                               <  2:772777/150‑1 (MQ=255)
                       aTAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGt                                                                                                                               >  2:4169702/1‑150 (MQ=255)
                       aTAGGTGAACGCCCACAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGt                                                                                                                               <  1:1709756/150‑1 (MQ=255)
                        tAGGTGAACGCCCGCAACGGAGGAGAAAGGCCAGCACAGGGTCGTTTTTCCTGTCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTc                                                                                                                              <  2:3521243/150‑1 (MQ=255)
                        tAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGGACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCTGCCGAGGAAGCGTTCCAGTc                                                                                                                              >  1:459550/1‑150 (MQ=255)
                        tAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTc                                                                                                                              <  2:3630156/150‑1 (MQ=255)
                          ggTGCACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCtt                                                                                                                            <  1:141237/150‑1 (MQ=255)
                          ggTGAACGCCCGCAAGGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCtt                                                                                                                            <  2:3828336/150‑1 (MQ=255)
                          ggTGAACGCCCGCAACGGAGGAGAAAGAACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCtt                                                                                                                            <  2:480164/150‑1 (MQ=255)
                          gatGAACTCCCGCAACGGAGAAGAAAGTACAGCACAGTTTCTTTTTTCTTGCCTTCCATCCCATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCTTATTTATAATACAAAGGAATGCGCCTGCCGCCGAGAAGGCGTTCCAGTCtt                                                                                                                            <  2:2892846/148‑1 (MQ=255)
                           gtcaaCCCCCGCATCGGAGGAGAAAGGACAGCCCAGGATCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTAAACAGCGCTGATCTCGTGTCCTTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCttt                                                                                                                           <  1:2530937/147‑1 (MQ=255)
                                cGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTACAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCa                                                                                                                      <  2:327785/150‑1 (MQ=255)
                                 gCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCaa                                                                                                                     >  1:4357835/1‑150 (MQ=255)
                                 gCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTCCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCaa                                                                                                                     <  2:3993208/150‑1 (MQ=255)
                                    cacAACGGAGGAGAAAGGACAGCACAGGGTCGTGTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAg                                                                                                                  >  2:3178630/3‑150 (MQ=255)
                                    cGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAg                                                                                                                  >  2:2834976/1‑150 (MQ=255)
                                     gCAAGGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGt                                                                                                                 <  2:2145615/150‑1 (MQ=255)
                                     gCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGt                                                                                                                 <  2:1796774/150‑1 (MQ=255)
                                       aaCGGAGGAGAAAGGACAGCACAGGTTCGTTTTCCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATTCGGCCGCCGCCGAGAAGGCGTTGCAGTGTTTTATCAACAGTCa                                                                                                               <  2:3663157/150‑1 (MQ=255)
                                               agaAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAATAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATAc                                                                                                       >  1:809123/1‑150 (MQ=255)
                                                 aaaGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACAt                                                                                                     >  1:3655913/1‑150 (MQ=255)
                                                  aaGGACAGCACAGGGTCTTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATc                                                                                                    <  2:2318245/150‑1 (MQ=255)
                                                     gACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTa                                                                                                 <  2:4897277/150‑1 (MQ=255)
                                                        aGCACAGGGTCGTTTTTCTTGCCTTGCATCACTTCTTCAACGTAGTGAACAGCGCTGATCTGGTGTGCGTATTTATAATACAAAGGAATGCGTCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATtc                                                                                              <  1:4211462/150‑1 (MQ=255)
                                                        aGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATtc                                                                                              >  1:3750207/1‑150 (MQ=255)
                                                         gCACAGGGTCGTTTTCCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATtca                                                                                             <  2:1209801/150‑1 (MQ=255)
                                                             aGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAg                                                                                         >  1:843710/1‑150 (MQ=255)
                                                              gggTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGc                                                                                        >  1:3255605/1‑150 (MQ=255)
                                                                gTCGTTTTTCCTGCCTTCCATCACATCTTCACCGTACTGAACAGCGCTGATCTCGTGTGCGTTTTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCa                                                                                      <  1:2935850/150‑1 (MQ=255)
                                                                gTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCa                                                                                      >  1:4985542/1‑150 (MQ=255)
                                                                   gTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCAttt                                                                                   <  1:469317/150‑1 (MQ=255)
                                                                   gTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCAttt                                                                                   >  2:372849/1‑150 (MQ=255)
                                                                    ttttcCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCAtttt                                                                                  <  2:3071391/150‑1 (MQ=255)
                                                                      tttCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTcc                                                                                >  1:2219664/1‑150 (MQ=255)
                                                                      tttCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTcc                                                                                >  2:2042316/1‑150 (MQ=255)
                                                                       ttCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCg                                                                               >  1:1306551/1‑150 (MQ=255)
                                                                          cTGCCTTCCATCACATCTTCAAGGTACTGAACAGCGCTGATCTGGTGTGCGTATTTATAATAAAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATTAGCCATTTTCCGAtt                                                                            <  1:3366959/150‑1 (MQ=255)
                                                                             ccTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAGTGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCa                                                                         >  2:1905652/1‑150 (MQ=255)
                                                                               ttCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAg                                                                       >  1:982277/1‑150 (MQ=255)
                                                                                tCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGc                                                                      <  2:3893372/150‑1 (MQ=255)
                                                                                 ccATCACATCTTCAGCGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGGGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCt                                                                     <  1:4644121/150‑1 (MQ=255)
                                                                                  taTCCCATTTTCAACTTTTTGAACAGTGTTGTTCTCGTGTGTATATTTATAATTCAAAGAAATGCGTCTGCTCACGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCtt                                                                    <  2:4531079/149‑1 (MQ=255)
                                                                                  cATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTTTTTATAATACAAAGGAATGCGCCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCtt                                                                    <  1:3401885/150‑1 (MQ=255)
                                                                                    tCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTc                                                                  >  2:4340722/1‑150 (MQ=255)
                                                                                    tCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTc                                                                  <  1:1567309/150‑1 (MQ=255)
                                                                                         tCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCCATaa                                                             >  1:215514/1‑150 (MQ=255)
                                                                                                      gAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAg                                                <  2:1517344/150‑1 (MQ=255)
                                                                                                        aCAGCGTTGATCTGGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGaa                                              <  2:2474857/150‑1 (MQ=255)
                                                                                                        aCAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGaa                                              >  1:100852/1‑150 (MQ=255)
                                                                                                         cAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGaaa                                             <  1:4786392/150‑1 (MQ=255)
                                                                                                         cAGCGCTGATCTCGTGTGCGTATTAATAATACAAAGGAATGCGGCCGCCGCCGAGAACGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGaaa                                             <  1:3183985/150‑1 (MQ=255)
                                                                                                          aGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAat                                            <  2:4660214/150‑1 (MQ=255)
                                                                                                            cgcTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAatat                                          <  2:571456/150‑1 (MQ=255)
                                                                                                            cgcTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAatat                                          <  1:1962990/150‑1 (MQ=255)
                                                                                                              cTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATcc                                        >  1:3512569/1‑150 (MQ=255)
                                                                                                               tGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCa                                       >  2:610092/1‑150 (MQ=255)
                                                                                                                 aTCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACt                                     <  2:1395538/150‑1 (MQ=255)
                                                                                                                  tctgGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTa                                    <  2:4430559/150‑1 (MQ=255)
                                                                                                                  tctcGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTa                                    >  1:361748/1‑150 (MQ=255)
                                                                                                                    tggtgtGCGTATTTATAATACAAAGGAATGCGGCCCCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACa                                  <  1:2405842/148‑1 (MQ=255)
                                                                                                                     cGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACat                                 >  2:1725658/1‑150 (MQ=255)
                                                                                                                      gtgtGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACata                                <  2:4853654/150‑1 (MQ=255)
                                                                                                                       tgtgCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACatat                               <  2:4087762/150‑1 (MQ=255)
                                                                                                                       tgtgCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACatat                               >  1:647744/1‑150 (MQ=255)
                                                                                                                        gtgCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCACTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACAtata                              >  2:1269647/1‑150 (MQ=255)
                                                                                                                           cGTATTAATAATACAAAGGAAGGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAg                           <  2:136045/150‑1 (MQ=255)
                                                                                                                            gTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGt                          <  1:4848719/150‑1 (MQ=255)
                                                                                                                             tATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTa                         <  2:956066/150‑1 (MQ=255)
                                                                                                                              aTTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTAt                        >  2:1463424/1‑150 (MQ=255)
                                                                                                                                ttATAATACAAAGGAATGCGGCCGCCGCCGAGTAGGCGTGCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCCATTCCACGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTAttt                      >  2:4998553/1‑150 (MQ=255)
                                                                                                                                      taCAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCCGCTCATACATCGTATTCATCAGCCATATTCCGATTCCAAGCTTTCGATAATGAGTAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGa                >  1:3438088/1‑150 (MQ=255)
                                                                                                                                       aCAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAAGAGAAATATCCACTACATATACAGAATTTCCATAAg               >  2:3331886/1‑150 (MQ=255)
                                                                                                                                        cAAAGGAATGCGGCCCCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAgg              <  1:2073222/150‑1 (MQ=255)
                                                                                                                                           aGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTc           <  2:702689/150‑1 (MQ=255)
                                                                                                                                             gAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCAt         <  1:2820266/150‑1 (MQ=255)
                                                                                                                                              aaTGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATg        >  1:3056417/1‑150 (MQ=255)
                                                                                                                                               aTGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATGa       >  1:1354856/1‑150 (MQ=255)
                                                                                                                                               aTGCGGCCCCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATGa       <  2:1583332/150‑1 (MQ=255)
                                                                                                                                                tGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGAATCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATGAt      >  1:616342/1‑150 (MQ=255)
                                                                                                                                                 gCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATGAtt     >  1:3268924/1‑150 (MQ=255)
                                                                                                                                                  cGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATGAttt    >  2:3863538/1‑150 (MQ=255)
                                                                                                                                                   ggCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATGAtttt   >  1:4885142/1‑150 (MQ=255)
                                                                                                                                                    gccgccgccGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATGAttttt  <  2:843710/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCAAATAATTGCTGACTACACGAATAGGTGAACGCCCGCAACGGAGGAGAAAGGACAGCACAGGGTCGTTTTTCCTGCCTTCCATCACATCTTCAACGTACTGAACAGCGCTGATCTCGTGTGCGTATTTATAATACAAAGGAATGCGGCCGCCGCCGAGAAGGCGTTCCAGTCTTTTATCAACAGTCAGCTCATACATCGTATTCATCAGCCATTTTCCGATTCCAAGCTTTCGATAATGAGGAGAAACAGAAATATCCACTACATATAAAGTATTTCCATGAGGTTCATGATTTTT  >  BS168_reference_genome/1462887‑1463184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑