| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 1,749,907 | G→T | 31.1% | D164Y (GAC→TAC) | rnjB → | dual activity 5' exo‑and endoribonuclease J2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,749,907 | 0 | G | T | 31.1% | 482.9 / inf | 286 | D164Y (GAC→TAC) | rnjB | dual activity 5' exo‑and endoribonuclease J2 |
| Reads supporting (aligned to +/- strand): ref base G (128/69); new base T (58/31); total (186/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.71e-02 | |||||||||||
Only 100 of 295 total aligned reads displayed.
ACCGAAAAACTGATTTAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAGCGGCTGTCAGC > BS168_reference_genome/1749758‑1750056 | aCCGAAAAAGTGATTTAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCAATCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGgag < 2:477461/150‑1 (MQ=255)aCCGAAAAACTGATTTAAGAGAAATTCACTCCAAATCTGTTATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGgag < 2:1331306/150‑1 (MQ=255) gAAAAACTGATTTAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACt > 2:2501258/1‑150 (MQ=255) gAAAAACTGATTTAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACt > 1:449890/1‑150 (MQ=255) gAAAAACTGACTTAAGAGAAATTCACTCCAACTCTGTCATTACATTTGAATCAAGAAAGGTAGCGTTCTTTACAACGATTCACACCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACt > 1:551881/1‑150 (MQ=255) aaaaaCTGATTTAAGAGAAATTCACTCCAAATCTGTCACTACATTTGAATCAACACAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACtg < 2:335132/150‑2 (MQ=255) aCTGATTTAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATTGTTCTTTAGTACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTAGATCTATTGTATGCACGTGATACTTCaaa > 1:1082273/1‑150 (MQ=255) tGATTTAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAAtt < 2:1595785/150‑1 (MQ=255) tttAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGa > 1:1983212/1‑150 (MQ=255) ttAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGAc < 2:1147082/150‑1 (MQ=255) aaGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCa > 2:4956926/1‑150 (MQ=255) gagaAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCaaa > 1:1572699/1‑150 (MQ=255) gaAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAaca > 1:4712108/1‑150 (MQ=255) aaaTTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAacac > 2:3201373/1‑150 (MQ=255) aaaTTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAACTTTGACCAAacac > 2:3434929/1‑150 (MQ=255) aaTTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACAcc > 2:3632703/1‑150 (MQ=255) cTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACt > 2:1509497/1‑150 (MQ=255) cTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACt > 2:1811637/1‑150 (MQ=255) cAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTaa > 2:298580/1‑150 (MQ=255) cAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCCTTAAAACAGATNTTTCGTCTCTTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTag > 1:2888234/1‑149 (MQ=255) aaTCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATc > 1:497480/1‑150 (MQ=255) tGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAgtc > 1:66506/1‑148 (MQ=255) tGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGAc > 2:3270334/1‑150 (MQ=255) gTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACg > 2:721093/1‑150 (MQ=255) gTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACg > 1:4650401/1‑150 (MQ=255) tCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGAATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGt > 2:1037839/1‑150 (MQ=255) cATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTg < 2:1085493/150‑1 (MQ=255) aTTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACAt > 2:4845465/1‑150 (MQ=255) tttGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACAtt < 1:4203140/150‑1 (MQ=255) tGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTgg > 1:4801052/1‑150 (MQ=255) tGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTgg < 2:4819570/150‑1 (MQ=255) gAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGc > 2:3821206/1‑150 (MQ=255) aaTCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCg > 2:2387620/1‑150 (MQ=255) tCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCgag > 2:3151826/1‑150 (MQ=255) caacaaAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGCAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCgaga > 1:121802/1‑150 (MQ=255) aaGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGcc > 1:11650/1‑150 (MQ=255) aGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGAGAGATCGCCa > 1:1494456/1‑150 (MQ=255) tATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGa > 1:1025754/1‑150 (MQ=255) aTCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGAt < 2:2590067/150‑1 (MQ=255) tCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATc < 2:2435732/150‑1 (MQ=255) gTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCAGTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGGGACATTGGCGAGATCGCCAAGATCgg > 1:3418566/1‑150 (MQ=255) gTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCgg > 1:3551700/1‑150 (MQ=255) ttCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGc > 2:2869996/1‑150 (MQ=255) tCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCa > 1:2146207/1‑150 (MQ=255) tttAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAAt < 1:4878221/150‑1 (MQ=255) aGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAgc > 2:4138401/1‑150 (MQ=255) gAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAgcg < 2:3748718/150‑1 (MQ=255) aCGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAgcggc > 1:2980463/1‑150 (MQ=255) gATTCACAGCATGCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAGACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGt < 1:4329959/150‑1 (MQ=255) ttCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTgc > 1:3117084/1‑150 (MQ=255) cacaGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTgctc < 1:4162203/150‑1 (MQ=255) aGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTgctcgct > 1:4485286/1‑150 (MQ=255) cATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGctct < 2:1181325/150‑1 (MQ=255) cATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGctct < 1:1759661/150‑1 (MQ=255) aTTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTg > 2:542802/1‑150 (MQ=255) tCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTgat < 1:956040/150‑3 (MQ=255) gATTCTGTAGGTGTGAGCTTTAAAACATCTCTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCt < 2:3594888/150‑1 (MQ=255) gATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGANCGGCAATAGCGGCGTGCTCGCTCTGCTTTCt > 1:1615310/1‑150 (MQ=255) tCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGAc < 1:2707884/150‑1 (MQ=255) tCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGAc < 2:1965361/150‑1 (MQ=255) tCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGAc < 2:4384204/150‑1 (MQ=255) tCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGAc > 2:4922328/1‑150 (MQ=255) tGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATACCGGCGTGCTCGCTCTGCTTTCTGACAg < 1:2078455/150‑1 (MQ=255) gTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGt > 2:3249665/1‑150 (MQ=255) tAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTg > 1:3215481/1‑150 (MQ=255) aGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGc > 2:273137/1‑150 (MQ=255) aGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGc > 2:4333610/1‑150 (MQ=255) gtgtGAGCTTTAAAACATCACTTGGATCTATTGAATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCaa > 1:3068914/1‑150 (MQ=255) gtgAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAAt < 2:918892/150‑1 (MQ=255) tgAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATg < 1:3945426/150‑1 (MQ=255) tgAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGTCATTGGGGAGATCGCCAAGATCGACAATAGCGGCGTGCTCGCTCTGCTTTCTGTCAGTGCAAATg > 1:455186/1‑150 (MQ=255) tgAACTTTAAATCATCACTTGGATCTATTGTATGCACGGGTGACTTCAAATGTGACCAAACACCTGCACTTAATCAGATGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATg > 1:3680217/1‑150 (MQ=255) gAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGc < 2:3069193/150‑1 (MQ=255) accTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCg < 1:3355351/148‑1 (MQ=255) tttAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGaa > 2:4024219/1‑150 (MQ=255) tAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACg > 1:2998654/1‑150 (MQ=255) aaaaCATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGTACAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACgg < 1:3235350/150‑1 (MQ=255) tCACTTGGATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGt > 1:8563/1‑150 (MQ=255) tCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGt > 1:1927534/1‑150 (MQ=255) cACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGtt > 2:3678445/1‑150 (MQ=255) aCTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTa < 2:3556420/150‑1 (MQ=255) ggATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACAcc > 1:2323635/1‑150 (MQ=255) gATCTATTGTATGCACGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACAccc > 1:4940919/1‑150 (MQ=255) tCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCt > 2:4203053/1‑150 (MQ=255) cTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCtt > 1:651409/1‑150 (MQ=255) cTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCtt > 2:2800037/1‑150 (MQ=255) tATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTc < 1:3268441/150‑1 (MQ=255) ttGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCgg > 2:4463232/1‑150 (MQ=255) ttGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCgg < 2:652893/150‑1 (MQ=255) tGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGa > 1:2357003/1‑150 (MQ=255) tATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAg < 1:802882/150‑1 (MQ=255) tATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGACCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAg < 1:1257405/150‑1 (MQ=255) gCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAGCgg < 2:449890/150‑1 (MQ=255) gCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAGCgg < 2:587513/150‑1 (MQ=255) cACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAGCGGc < 2:844525/150‑1 (MQ=255) cACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAGCGGc > 1:4411131/1‑150 (MQ=255) cGGGATACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAGCGGCTg > 2:1866405/1‑150 (MQ=255) ggAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGACCGGGTTACACCCCTTCGGAAGCGGCTGTc > 2:30914/1‑150 (MQ=255) gaCTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAGCGGCTGTCAGc > 2:1730415/1‑150 (MQ=255) gaCATCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACTCCCCTTCGGAAGCGGCTGTCAGc > 1:2461548/1‑150 (MQ=255) | ACCGAAAAACTGATTTAAGAGAAATTCACTCCAAATCTGTCATTACATTTGAATCAACAAAGGTATCGTTCTTTAGAACGATTCACAGCATTCCTGATTCTGTAGGTGTGAGCTTTAAAACATCACTTGGATCTATTGTATGCACGGGAGACTTCAAATTTGACCAAACACCTGCACTTAATCAGACGTGCGACATTGGCGAGATCGCCAAGATCGGCAATAGCGGCGTGCTCGCTCTGCTTTCTGACAGTGCAAATGCGGAACGGCCGGGTTACACCCCTTCGGAAGCGGCTGTCAGC > BS168_reference_genome/1749758‑1750056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |