BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	3,532,283	C→G	9.6%	P59P (CCC→CCG)	BSU_34399 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	3,532,283	0	C	G	9.6%	365.4 / 8.6	221	P59P (CCC→CCG)	BSU_34399	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (88/80); new base G (3/23); total (113/108)
Fisher's exact test for biased strand distribution p-value = 8.28e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

GCCATTGTTGTGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTCCGC  >  BS168_reference_genome/3532134‑3532429

    ttgttgTGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑aa                                                                                                                                                  <  2:1364953/150‑1 (MQ=255)

      gttgtGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑aaaa                                                                                                                                                <  1:4434987/150‑1 (MQ=255)

        tgtgGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACACTG‑aaaaaa                                                                                                                                              >  1:1369114/1‑148 (MQ=255)

        tgtgGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACCGG‑AAAaga                                                                                                                                              >  2:4451873/1‑150 (MQ=255)

         gtgGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCGGG‑AAAagag                                                                                                                                             >  1:296973/1‑150 (MQ=255)

             cAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGt                                                                                                                                         <  2:2402131/150‑1 (MQ=255)

              aGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACCGG‑AAAAGAAAAATa                                                                                                                                        >  1:564133/1‑150 (MQ=255)

                cTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAAt                                                                                                                                      <  2:4637852/150‑1 (MQ=255)

                 ttGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAAtt                                                                                                                                     <  2:2498782/150‑1 (MQ=255)

                  tGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGG‑AAAAGAGAAGTAATtc                                                                                                                                    >  1:4586348/1‑150 (MQ=255)

                   gTATCATTATTTTGTCGTCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGAACTGGCATCATACTGTTTTTC‑GCGGCAAATGTTATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGG‑AAAAATACAGTAATtct                                                                                                                                   >  1:2322855/1‑150 (MQ=255)

                    tATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTTGACTGACATCATACTGTTTTTC‑GCGGCAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACAGG‑AAAagaaaagaaactatc                                                                                                                                  >  1:2269787/1‑145 (MQ=255)

                           attTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCg                                                                                                                           <  1:2744944/150‑1 (MQ=255)

                           attTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCg                                                                                                                           <  1:4087636/150‑1 (MQ=255)

                           attTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTAGCATCATACTGTTTTTC‑GCGGGAATGGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAAACGG‑AAAAGAGTAGTAATTCTCTTTTCCg                                                                                                                           >  2:3895815/1‑150 (MQ=255)

                            ttttGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGAAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTTTTTTCCgt                                                                                                                          <  2:1001115/150‑2 (MQ=255)

                             tttGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCg‑g‑g                                                                                                                       <  2:2103966/150‑1 (MQ=255)

                             tttGCCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCCCTGGACTGGCATCATCCTTTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCg‑g‑g                                                                                                                       <  1:1076729/150‑1 (MQ=255)

                              ttttgggCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACACAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑Gt                                                                                                                      <  1:1093480/145‑1 (MQ=255)

                                   ggCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTa                                                                                                                 <  1:2112755/150‑1 (MQ=255)

                                    gcggTGGTATGTTTACGGGACACAGCCTATTCCCAGCCTATGCGCTGGACTGGCTCCATACTGTTTTTC‑GCGGGAATTGTGAGGGTTCCCGCATACTCAGCAAAAAGAAACCCCGG‑AAAAGAGAAGTAATTCTTTTTCCCA‑G‑GTTTTTAt                                                                                                                <  2:330181/147‑1 (MQ=255)

                                    gCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTAt                                                                                                                >  1:3884818/1‑150 (MQ=255)

                                     ccGTGGTTTGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGCCTGGCATCTTACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGAAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATg                                                                                                               <  1:834060/150‑1 (MQ=255)

                                     ccGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACACGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTTTg                                                                                                               >  1:3241038/1‑150 (MQ=255)

                                           tATGTTTCCGGGACACAGCCTATTACCAGCCTATGCCCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATCCCCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTCCCG‑G‑GTTTTTATGTttatt                                                                                                         <  2:3441823/150‑1 (MQ=255)

                                            aTGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTttatta                                                                                                        >  2:2858058/1‑150 (MQ=255)

                                              gTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCACCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTAAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCT‑G‑GTTTTTATGTTTATtata                                                                                                      <  1:2616233/150‑1 (MQ=255)

                                                ttACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACAAGG‑GAAAGAGAAAAAATTCTCTTTTCCC‑G‑GTTTTTTTTTTTTTTTTAAt                                                                                                    >  1:5005921/1‑150 (MQ=255)

                                                taaggggACACAGTTTATTACAAGCCTATGCGTTGGTCTGGCACCAT‑CTTTTTTTCGGCGGGATTTGTGATGGTCCCCGCAACCCCAGAAAAAAAAAAACCGGA‑AAAAGAGAGGTAATTCTCTTTCCCG‑G‑GTTTTTATGTTTATTATAAt                                                                                                    <  2:1150977/146‑1 (MQ=255)

                                                 tACGGGACACAGTCTATTACCAGCCTTTGCGCTGGACTGGCATCATACTGTTTTTC‑GGGGGAATTGTGTTGGTCCCCGCATACCCAGCAAAAAGAAACCCCGG‑AAAAGAGAAGTATTTCTTTTTTCCG‑G‑GTTTTTATGTTTATTATAATc                                                                                                   <  2:3297334/150‑1 (MQ=255)

                                                 tACGGCACCCAGTCTATTACCAGCCTGTGCGCGGGACTGGCATCATACTGTTTTTC‑GGGGAATTTGTGATGGTCCCCGCATACCCAGCAAAAAAAAAACCGGG‑AAAAGAGAAGTATTTCTCTTTCCGG‑G‑GTTTTTATGTTTATTATAATc                                                                                                   <  2:4995154/150‑1 (MQ=255)

                                                  aCGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACACAG‑AAAAAAGAAGTAATTCTCTTTTCCT‑G‑GTTTTTATGTTTATTATAATCt                                                                                                  >  1:4748432/1‑150 (MQ=255)

                                                   cGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCtt                                                                                                 >  2:4853982/1‑150 (MQ=255)

                                                    gggACACAGTCTATTCCAAGCCTATGCGCTGGACTGGCATCATCCTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTc                                                                                                <  2:615192/150‑1 (MQ=255)

                                                     ggACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACCGG‑AAAAGAGAAGTAATTCTCTTTTTCC‑G‑GTTTTTATGTTTATTATAATCtttc                                                                                               >  1:4706287/1‑148 (MQ=255)

                                                         acaGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCcgcg                                                                                           >  2:1804007/1‑150 (MQ=255)

                                                         acaGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGG‑GAAAGAAAAATAAATTTCTTTTCCC‑G‑GGTTTTATGTTTATTATAATCTTCCcgct                                                                                           >  1:1486666/1‑149 (MQ=255)

                                                           aGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCcgcgcg                                                                                         >  2:1186343/1‑150 (MQ=255)

                                                           aGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACAGG‑ATAAGAGAAGTAAATCTCTTTTTCG‑G‑GTTTTTATGTTTTTTTGAATCTTGCcgcgct                                                                                         >  1:842771/1‑149 (MQ=255)

                                                             tCTATTACCAGCCTATGCGCTGGACTGACATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCGGG‑AAAAGAGAAGTAATTCTCTTTTCCC‑G‑TTTTTTATGTTTATTATAAACTTCCCCCGCGaa                                                                                       >  1:2477852/1‑150 (MQ=255)

                                                               tATTACCAGCCTATGCGCGGGCCGGCCACCATATTTTTTTTC‑GCGGGAATTGTGATGGTTCCCCCATACTCAGCAAAAAGAAAACCCGA‑AAAAGAGAAGTAATTCTCTTTCCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAata                                                                                     <  1:665832/150‑1 (MQ=255)

                                                                 ttACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTCCCCGCATACTCAGCAAAAAGAAAACCGGG‑AAAAGAGAAGTAATTCTCTTTCCCG‑A‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATc                                                                                   <  1:432450/150‑1 (MQ=255)

                                                                 tacccAGCCTATGCGCGGGATTGCAATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAACAAAAAAAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTCCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATc                                                                                   <  1:1130946/147‑1 (MQ=255)

                                                                    ccAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGtt                                                                                >  1:156245/1‑149 (MQ=255)

                                                                     cAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTCCCCGCAAACTCAACAAAAAGAAAACCCGG‑AAAAGAGAAGAATTTCTCTTTCCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCt                                                                               <  1:1677446/150‑1 (MQ=255)

                                                                      aGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTTTAATCTTCCCCCGCGAATATCGTCTg                                                                              >  1:1874326/1‑150 (MQ=255)

                                                                       gCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACAGG‑GAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTTTGTTTTTTTTAATTCTCCCGCGCGAATATCCTCTGt                                                                             >  1:467517/1‑150 (MQ=255)

                                                                        ccTATGCGGTGGACTGGCAACATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACACAGCAAAAAGAAAACCGGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑T‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTc                                                                            <  1:2230760/150‑1 (MQ=255)

                                                                              cgcTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTccc                                                                      >  1:3136312/1‑150 (MQ=255)

                                                                               gcTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCTGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATTTTTATTATAATCTTCCCCCGGGAATATCCTCTGTCATTCCCt                                                                     >  1:4507231/1‑150 (MQ=255)

                                                                               gcTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGG‑GAAAGAGAAGAAAATTTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTTCCGCGCGAATATCGTTTGTCAATTCCt                                                                     >  1:3341827/1‑150 (MQ=255)

                                                                                 tGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCa                                                                   <  2:1867944/150‑1 (MQ=255)

                                                                                 nggANTGGCATCATACTGTTTTTC‑GNGGGAATTGTGATGGTTCCCGCACNATCAGCAAANAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCa                                                                   >  1:3497391/2‑150 (MQ=255)

                                                                                     cTGGCATCATCTTGTTTTTC‑GCGGAAATTGTGATGGTCCCCGCATACACAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAg                                                               <  2:1874326/150‑1 (MQ=255)

                                                                                      tGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAgg                                                              >  1:3713697/1‑150 (MQ=255)

                                                                                       ggCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACACAGCAAAAAGAAAACCCGA‑AAAAGAGAAGTAATTCTTTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGa                                                             <  1:1733879/150‑1 (MQ=255)

                                                                                          atcatTGTGTTTTTC‑GGGGGAATTGTGATGGCCCCCGCATACACAGCAAAAAAAAAACCCGG‑AAAAGAGAAGTAATTCT‑TTTTCCG‑GAGTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGcc                                                          <  2:4919604/150‑1 (MQ=255)

                                                                                          atcatACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAAACCGG‑AAAAGAGAAGTAATTCTTTTTTCCG‑G‑GTTTTTATGTTTATTTTAATCTTCCCGCGGGAATATCGGCTGTCATTCCCTCATAAGGAGct                                                          >  1:3310085/1‑149 (MQ=255)

                                                                                            catACTGTTTTTC‑GCGGGAATTGTGATGGTCCCCGCAAACACAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCtt                                                        <  2:3469707/150‑1 (MQ=255)

                                                                                             atattttttttC‑GGGGGATTTGTGTTGGTCCCCGAATACAAAGAAAAAAGAAACCCCGG‑AAAAGAAAATTAATTCTCTTTCCAG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTc                                                       <  1:3910786/144‑1 (MQ=255)

                                                                                             atatTGTTTTAC‑GGGGGTATTGTGATGGTCCCCCCATCAACAGCAAAAAAAAACCGAGG‑AAAAAAAAACTAATTCTTTTTCCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTc                                                       <  1:1832435/150‑1 (MQ=255)

                                                                                             atACTTTTTTTC‑GGGGGAATTGTGATGGTTCCCGCATACACAGCAAAAAGAAAACCGGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTc                                                       <  2:2461646/150‑1 (MQ=255)

                                                                                              tACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTTCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTcc                                                      >  2:1375092/1‑149 (MQ=255)

                                                                                                  ttttttC‑GGGGGAATTGTGTTGGTTCCCGCATACTCAGCAAAAAAAAAACCGGG‑AAAAGAGAAGTAATTCTCTTTTCCT‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAAt                                                  <  2:559047/149‑1 (MQ=255)

                                                                                                  gTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTTCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAAt                                                  >  1:2147876/1‑150 (MQ=255)

                                                                                                   tttttC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑ATTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTTTGTCATTCCCTCATAAGGAACCTTTGAAATc                                                 >  2:4369678/1‑150 (MQ=255)

                                                                                                        c‑gcgGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCTGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCActtc                                            >  1:4214665/1‑150 (MQ=255)

                                                                                                          gggggAATTGTGATGGTCTCCGCATCCACAGAAAAAAAAAAACCAGA‑AAAAGAGAATTATTTCTTTTTTCTT‑T‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCActtct                                           <  1:4828424/148‑1 (MQ=255)

                                                                                                           cgGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCActtctt                                          <  2:3733577/150‑1 (MQ=255)

                                                                                                            ggttatcgtgATGCTTCCCACATACTCAAAAAAACGAAAACACGG‑AAAAATCATTTTTTTCT‑TTTTCCGTT‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACttcttc                                         <  1:1915257/143‑1 (MQ=255)

                                                                                                            gggATTTGTGATGGTTCCCGCATACCCAGCAAAAGGAAACCCGGA‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACttcttc                                         <  2:310061/150‑1 (MQ=255)

                                                                                                            gggAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACttcttc                                         >  2:2766471/1‑150 (MQ=255)

                                                                                                            gggAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCAGG‑AAAAGAGAAGTAATTCTCTTTTCCT‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACttcttc                                         <  2:323062/150‑1 (MQ=255)

                                                                                                            gggAATTGTGATGGTCCCCGCATCCTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACttcttc                                         <  2:756204/150‑1 (MQ=255)

                                                                                                             ggATTTGTGATGGTTCCCCCATACTCAGCAAAAAAAAAACCAGA‑AAAAGAGAAGTATTTCTTTTTTCAT‑A‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCg                                        <  1:4004300/150‑1 (MQ=255)

                                                                                                               agttGTGATGGTTCCCACATACACA‑AAAAAAAAAAACCCGGAAAAAGAGAAGTAATTCTTTTCTCTT‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGtt                                      <  1:2858058/148‑1 (MQ=255)

                                                                                                                aTTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑C‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTg                                     >  1:363825/1‑150 (MQ=255)

                                                                                                                   gtgATGGTCCCCGCATACACAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTCCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCg                                  <  2:1479872/150‑1 (MQ=255)

                                                                                                                    tgATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGa                                 >  2:878062/1‑150 (MQ=255)

                                                                                                                    tgATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGa                                 >  2:2040337/1‑150 (MQ=255)

                                                                                                                     gATGGTTCCCCCATACACAGAAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTCCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGAc                                <  1:3423198/150‑1 (MQ=255)

                                                                                                                     gATGGTCCCCCCAAACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTATTTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGAc                                <  2:336830/150‑1 (MQ=255)

                                                                                                                       tGGTTCCCGCATACTCAGCAAAAAGAAAACCGGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAAACACTTCTTCGTTGTCGACTc                              >  1:1850669/1‑150 (MQ=255)

                                                                                                                         gtccccGCATACTCAGAAAAAAGAAAACCCGA‑AAAAGAGAAGTAATTCTCTTTCCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCt                            <  2:4706287/147‑1 (MQ=255)

                                                                                                                          ttCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTTCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCCCGTATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCtt                           >  1:4765488/1‑150 (MQ=255)

                                                                                                                           tCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTc                          >  1:901148/1‑150 (MQ=255)

                                                                                                                            cccGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCa                         <  2:4153377/150‑1 (MQ=255)

                                                                                                                                cATACCCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCAtttt                     <  2:2182227/150‑1 (MQ=255)

                                                                                                                                 aTACTCAGCAAAAAGAAAACGTGG‑AAAAGAAAAGTAATTCTCTTTTCCG‑G‑TTTTTGATGTTTATTATAATTTTCCCGAGCGAATATCGTCTATCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCAttttt                    >  1:269955/1‑150 (MQ=255)

                                                                                                                                  tACACAGCAAAAAAAAAACCGGA‑AAAAGAGATTTATTTCTTTTTCCTG‑T‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTc                   <  2:1511632/150‑1 (MQ=255)

                                                                                                                                      cAGCAAAAAGAAAACCCGG‑GAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGaa               >  2:3719149/1‑150 (MQ=255)

                                                                                                                                       acaaaaaaaaaaaCCCGG‑AAAAGAGAATTAATTCTCTTTCCGT‑T‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGaaa              <  2:837951/147‑1 (MQ=255)

                                                                                                                                       aGCAAAAAGAAACCCCGG‑AAAAGAGAAGTATTTCTCTTCTCCG‑T‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGaaa              <  2:3959801/150‑1 (MQ=255)

                                                                                                                                       aGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGaaa              >  2:2375690/1‑150 (MQ=255)

                                                                                                                                         cAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCTGAAATg            >  2:1720268/1‑150 (MQ=255)

                                                                                                                                           aaaaGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTa          >  2:3538372/1‑150 (MQ=255)

                                                                                                                                           aaaaGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTa          >  1:2716176/1‑150 (MQ=255)

                                                                                                                                                  aaCCCGG‑AAAAGAGAAGTATTTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTCCg   <  2:4586348/150‑1 (MQ=255)

                                                                                                                                                  aaCCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTCCg   >  2:5016155/1‑150 (MQ=255)

                                                                                                                                                   aCCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCTGAAATGTAATTTCCGc  >  1:621695/1‑150 (MQ=255)

GCCATTGTTGTGGCAGCTTGTATCATTATTTTGTCGGCCGTGGTATGTTTACGGGACACAGTCTATTACCAGCCTATGCGCTGGACTGGCATCATACTGTTTTTC‑GCGGGAATTGTGATGGTTCCCGCATACTCAGCAAAAAGAAAACCCGG‑AAAAGAGAAGTAATTCTCTTTTCCG‑G‑GTTTTTATGTTTATTATAATCTTCCCGCGCGAATATCGTCTGTCATTCCCTCATAAGGAGCCTTCGAAATCACTTCTTCGTTGTCGACTCCTTCATTTTTCAGAAATGTAATTTCCGC  >  BS168_reference_genome/3532134‑3532429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`