| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 3,807,717 | C→A | 11.4% | intergenic (‑342/+37) | murAB ← / ← tal | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase/transaldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 3,807,717 | 0 | C | A | 11.4% | 703.1 / 10.7 | 273 | intergenic (‑342/+37) | murAB/tal | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase/transaldolase |
| Reads supporting (aligned to +/- strand): ref base C (38/166); new base A (36/2); total (104/169) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.06e-20 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 287 total aligned reads displayed.
GGCTCGAGAGACATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAG > BS168_reference_genome/3807568‑3807866 | tCGAGAGACATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGc > 2:1950215/1‑150 (MQ=255) cGAGAGACATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGTGGCa > 1:462889/1‑150 (MQ=255) cGAGAGACATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCa < 2:3025467/150‑1 (MQ=255) gagagaCATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGGAATACAAAGGCCGGGGcca > 2:2240592/1‑148 (MQ=255) gagagaCATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCgggca > 1:4303131/1‑147 (MQ=255) agagaCATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCcgcgagaca > 1:2366994/1‑145 (MQ=255) gagaCATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAAc < 1:1203831/150‑1 (MQ=255) agaCATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACa < 1:1581382/150‑1 (MQ=255) gaCATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGGaaaaac > 1:2665583/1‑147 (MQ=255) aCATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGGaaaaaag > 2:1417070/1‑146 (MQ=255) aTCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAAAACAAAAGCCAAGGAAAAAAggaa > 2:2839957/1‑148 (MQ=255) tcgtcgTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCAt < 2:4013421/150‑1 (MQ=255) tcgtcgTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCAt < 2:3081084/150‑1 (MQ=255) cgtcATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAg < 2:1095718/150‑1 (MQ=255) tcATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCt < 1:5003503/150‑1 (MQ=255) tcATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCt > 2:4495240/1‑150 (MQ=255) tcATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCNGAAGATCCGNTGTATCAGGTCGGTGAAATACAANGGCCGCGGCAAACAGGCATANAGcn < 1:3489585/150‑2 (MQ=255) cATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGAGATAAAACTg > 2:3795279/1‑150 (MQ=255) tCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑tt < 1:4699355/150‑1 (MQ=255) ccGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑ttt < 2:4400985/150‑1 (MQ=255) gccaaaGAAGTTCGCAAACCCTTCATTCGGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTgcc < 1:1439157/147‑1 (MQ=255) cAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCtt < 1:269306/150‑1 (MQ=255) cAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGACCCGCTGTACCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCtt < 1:4884205/150‑1 (MQ=255) aGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCttt < 2:3439154/150‑1 (MQ=255) gTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTTGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTc < 2:511368/150‑1 (MQ=255) gTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTc < 2:2041204/150‑1 (MQ=255) ccGCATACCCTTCATTCCGATATCAGCTTCTCCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTACCAGGCCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCTCCCGTTTCat < 2:1510732/149‑1 (MQ=255) aCGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGGAAAAAGGGATAAAGCTG‑TTTGGCGCCCCCTTCat > 2:654140/1‑150 (MQ=255) cGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCatt < 2:2277626/150‑1 (MQ=255) cccTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGt < 1:2456522/150‑1 (MQ=255) cTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTc < 2:1870786/150‑1 (MQ=255) cATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAg < 2:3539517/150‑1 (MQ=255) cATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAg < 2:1181550/150‑1 (MQ=255) cATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGGAAACAGGAAAAAAAATG‑TGTGGCGCCCCCTTCCTTATTTTTTTCAg > 1:112644/1‑150 (MQ=255) aTTCCGATACCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGt < 2:3213447/150‑1 (MQ=255) ccGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGCAAACAAGCATAAAGCTG‑TTTGCCGCCCCCTTCATTATTTGTTCCAGTCTg > 2:3497905/1‑150 (MQ=255) gATATCAGCTTGTGCAGCGCAATCATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAATATGCGTTGTATCAGGTAAGTGAAATACAAACGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGcc < 1:4386076/150‑1 (MQ=255) atatCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGTAAACAGGGGAAAAGCTG‑TGGTACGCCCCCTTCTTTTTTTTTTTCACTCTcccc > 1:169054/1‑149 (MQ=255) atatCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCa < 2:942753/150‑1 (MQ=255) tatCAGCTTCTGCAGCCCAATGATTCCTTCTGTGTAAAGAGGCGTCAGCAGCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAg < 1:3712115/150‑1 (MQ=255) tatCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAg < 1:2248063/150‑1 (MQ=255) tatCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGGAAACAGGCATAAAAATGTTTTGTCGCCCC‑CTCCTTTTTTTTTTCTGTCTGcccg > 2:3276954/1‑148 (MQ=255) atCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGGGGCCAACAGGCATAAAGGTG‑TTTGCCGCCCCCTTCCTTTTTTGTTTCCGTCTTCCCgg > 2:4311359/1‑150 (MQ=255) cAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCCGCCAAAAAGAAAAAAACTG‑GTTTGCGCCCCCTTCCATATTTTTTCCTATCTGCCAAGaa > 1:1956108/1‑150 (MQ=255) aGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAAt < 2:1637342/150‑1 (MQ=255) ttCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGt < 2:4451683/150‑1 (MQ=255) tCTGCAGCCCAATGATTTCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGACCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTTCCCCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGtt < 2:2602049/150‑1 (MQ=255) tGCAGCACAATGATCCCTTCTTTGTAAAGCGGCGACAGCATCTGAAGATCCGCGGTATCAGGTCGGTGAAATACAAAGGTCGCGGCACACAGGCATAAAGCTG‑TTTGCCGCCGCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCg < 2:3999654/150‑1 (MQ=255) cAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATGTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGAt < 1:3678104/150‑1 (MQ=255) gCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATTCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCACCTCTTTCATTATTTGTTTCAGTCTGCCAGGAATTGTTCGATTc < 1:2627167/150‑1 (MQ=255) cccAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGGGGCAAAGAGGCATAAAGCTG‑TTTGCCGCCCCCTTCATTATTTGGTCCCGGCTGCCCGGGAATGTTCGCTTcc > 2:136222/1‑150 (MQ=255) cAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCtt < 2:4860947/150‑1 (MQ=255) cAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTACCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGCCATAAAGCTG‑TTTGCCACCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCtt < 1:3009292/150‑1 (MQ=255) aaTGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCttt < 2:4053563/150‑1 (MQ=255) aaTGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCttt < 1:672526/150‑1 (MQ=255) aaTGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGCAAACAGGCATAAAACTG‑TTTGCCGCCCCCTTCATTATTTGTTTCTGTCTGCCAGGAATTGTTCGGTTCCttt > 2:790682/1‑150 (MQ=255) ttCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGGGGGAAAAAGGGATAAAACAG‑TTTGCCGCCCCCTTCATTATTTTTTTCAGTCTGCCAGGGAATGTTTCGTTCCTTtgtttgttta > 2:2516898/1‑148 (MQ=255) ttCTCTGTAAAGCGGCGACAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTaa < 1:542129/150‑1 (MQ=255) tgtctGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAAc < 2:3682141/148‑1 (MQ=255) tctcTGTAAAGTGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAAc < 1:601750/150‑1 (MQ=255) tctcTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAAc < 1:1911767/150‑1 (MQ=255) tctcTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAAc < 1:2899555/150‑1 (MQ=255) tctGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGATG‑TTTGTCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAAATGTTCGATTCCTTTGTCTGTTAACgg > 2:2436676/1‑150 (MQ=255) ctGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACggg < 1:3910295/150‑1 (MQ=255) ctGTAAAGCGGCGCCAGCATCTGAAGAGCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGCCTGTTAACggg < 2:3583940/150‑1 (MQ=255) aaaGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGtt < 2:4725015/150‑1 (MQ=255) aGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTNAGTATTTGTNCCAGTCTGCCAGGAATTGTTCGANTCCTTTGTCTGTTAACGGGTNtttn < 1:3499256/150‑2 (MQ=255) ggcgTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTg < 2:2394763/150‑1 (MQ=255) tCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAgcg < 2:599085/150‑1 (MQ=255) aGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGGAAACAGGCATAAAGCTG‑TTTGCCGGCCCCTTCATTATTTGTTTCAGTCTGCCAGGAATTGTTCGGTTCCTTTGTCTGGTAACCGGTGTTTAGTgggggga > 2:2169533/1‑144 (MQ=255) aGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCCTTAATTGTTTCACTCTGCCAGGAATTTTTTGATTCCTTTGTCTGTTTACGGGGGTTTTGTGGGGGCa > 2:3651869/1‑150 (MQ=255) aGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCa < 2:1564514/150‑1 (MQ=255) aTCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGCAAACAGGGATAAAGCTG‑TTTGCCGCCCCTTTCATTTTTTGTTCCAGTCCGCCAGGAATTGTTCGATTCCCTTGTCTGTTAACGGGTGGTTAGTGTGGGGatga > 1:3423314/1‑150 (MQ=255) tCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGGGGCAAAAAGGCATAAAGCTG‑TTTGGCGCCCCCTTCATTATTTGTTTCAATCTGCCAAGAATTTTTTGATTCCTTTGTTTGTTAAACGGTGTTTAAGTAGGGCatgaa > 1:4021340/1‑150 (MQ=255) tCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCatgaa < 2:1689479/150‑1 (MQ=255) tCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCAGAGGCAAAAAGACATAAAGCAG‑GTTGCAGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGGATTGTTGGATTTGTTTGTCATTTCACGGGGGGTTAGTGAGCGGatgga > 2:1544736/1‑148 (MQ=255) tGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCatgaat‑g < 2:3926881/150‑1 (MQ=255) tGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGCAAACAGGCATAAAACTG‑TTTGGCGCCCCCTTCATTATTTGTTTCAGTCTGCCAGCAAATGTTTGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCatgaat‑g > 1:3954941/1‑150 (MQ=255) ccGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCa < 1:91295/150‑1 (MQ=255) gCTGTATCAGGTCGGTGAAATACAAAGTCCGCGGCAAACAGGCATAAACCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGc < 1:3442828/150‑1 (MQ=255) gCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGc < 2:3183187/150‑1 (MQ=255) gCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAACTG‑GTTGCCGCCCCCTTCCTTATTTGTTTCAGTCTGCCAGGAATTGTTTGATTCCTTTTTCTTTTAACGGGTGTTTTGTGAGCGCATGAAA‑GACttttaac > 2:2783273/1‑146 (MQ=255) tATCAGGTCGGTGAAATACAAAGGCCGCGGGAAACAGGCATAAAGCTG‑TTTTCCGCCCCCTTCATTTATTTTTCCCGTCTGCCAAGGATTGTTCGATTCCCTTGTCTGTTTTAGGGTGTTTAGTGAGCGCATGAAT‑GGCTTTCAGCGGcc > 1:1055512/1‑149 (MQ=255) tATCAGGCTGGTGAAATACAAAGGCCGGGGCAAACAGGCATAAAGCTG‑TTTGCCACCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCa < 1:1808399/150‑1 (MQ=255) aTCAGGTCGGTGAAATACAAAGGCCGCGGCAAAAAGGCATAAAACTG‑TTTTCCGCCCCCTTCATTATTTTTTTCACTCTGCCCGGGATTGTTCGATTCCTTTTTCTGTTTACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGGGGGAt > 1:2977536/1‑150 (MQ=255) ggTCGGTGAAATACAAAGGCCGTGGCAAAAAAGCATAAAAATG‑TTTGCCGCCCCCTTCATTTTTTTTTTCAGTCTTGCCGGAATTGTTCGATTTCTTTTTTTGTTTACGGGTGTTTTGTGAGGGCATGGATGGACTTT‑AGCGGCATtgtg > 2:4363566/1‑150 (MQ=255) gAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGatat < 1:3790175/150‑1 (MQ=255) aaTACAAAGGCCGTGGCAAACAGGCATAAAACTG‑GTTGTCGCCCCCTTCCTTTTTTGTTCCAGGCTGGCACGAATTGGTTGATTCCTTTGTCTGTTAACGGGGGTTTTGTGGGGGCATGAAA‑GAGTTTCCGCGGCATTGTGGCGGTATgt > 2:3887356/1‑149 (MQ=255) aTACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAg < 1:790682/150‑1 (MQ=255) aCAAAGCCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCTCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGcc < 1:4495240/150‑1 (MQ=255) cAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGccc < 2:2082774/150‑1 (MQ=255) cAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGccc < 2:431894/150‑1 (MQ=255) aaGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCct < 1:385951/150‑1 (MQ=255) aaGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCct < 1:2201444/150‑1 (MQ=255) ggCCGCGGCAAACAGCCATAAAGCTG‑TTTGCCACCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCctct < 1:3120682/150‑1 (MQ=255) ccGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTaa > 2:3334104/1‑150 (MQ=255) cccgGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTGTCATTATTCGTTCAAGACTTTCAGGAGTGTCTCGATTCCGTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAag > 1:1877192/3‑150 (MQ=255) gcgGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAaga > 2:475084/1‑150 (MQ=255) cgGCAACCAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAagag < 2:3519205/150‑1 (MQ=255) cgGCAAACAGGCATAAAGCTG‑TTTGCCCCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAagag < 2:1784669/150‑1 (MQ=255) | GGCTCGAGAGACATCGTCGTCATCCGGCAAAAGCAGTACGCATACCCTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAAT‑GACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAG > BS168_reference_genome/3807568‑3807866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |