BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	3,807,763	C→T	14.5%	W210* (TGG→TGA)	tal ←	transaldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	3,807,763	0	C	T	14.5%	834.1 / 41.6	292	W210* (TGG→TGA)	tal	transaldolase
Reads supporting (aligned to +/- strand): ref base C (81/164); new base T (45/2); total (126/166)
Fisher's exact test for biased strand distribution p-value = 8.32e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 297 total aligned reads displayed.

CTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCAATGAT  >  BS168_reference_genome/3807614‑3807912

  tCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGGAAACAGGGATAAAAGTG‑TTTGGCGCCCCCTTCCTTTTTTGTTTCa                                                                                                                                                     >  2:2201444/1‑150 (MQ=255)

   cATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAg                                                                                                                                                    <  2:1995718/150‑1 (MQ=255)

   cATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAg                                                                                                                                                    <  2:3844820/150‑1 (MQ=255)

    aTTCCGATACCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGt                                                                                                                                                   <  2:3213447/150‑1 (MQ=255)

      tCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCt                                                                                                                                                 <  2:1056295/150‑1 (MQ=255)

       ccGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGCAAACAAGCATAAAGCTG‑TTTGCCGCCCCCTTCATTATTTGTTCCAGTCTg                                                                                                                                                >  2:3497905/1‑150 (MQ=255)

         gATATCAGCTTGTGCAGCGCAATCATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAATATGCGTTGTATCAGGTAAGTGAAATACAAACGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGcc                                                                                                                                              <  1:4386076/150‑1 (MQ=255)

           tatCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGGAAACAGGCATAAAAATGTTTTGTCGCCCC‑CTCCTTTTTTTTTTCTGTCTGcccg                                                                                                                                            >  2:3276954/1‑148 (MQ=255)

            atCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGGGGCCAACAGGCATAAAGGTG‑TTTGCCGCCCCCTTCCTTTTTTGTTTCCGTCTTCCCgg                                                                                                                                           >  2:4311359/1‑150 (MQ=255)

            atCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGCGGAAAAGAGATAAAGCTC‑TGTGGTGCGCCTTTCATTATTTGTTTTATTCTTCCAgg                                                                                                                                           >  1:3903893/1‑150 (MQ=255)

             tCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTAAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCGTTTCATTATTTGTTCCAGTCTGCCAGGa                                                                                                                                          <  1:3744486/150‑1 (MQ=255)

               aGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAAt                                                                                                                                        <  2:1637342/150‑1 (MQ=255)

                   tCTGCAGCCCAATGATTTCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGACCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTTCCCCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGtt                                                                                                                                    <  2:2602049/150‑1 (MQ=255)

                     tGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCg                                                                                                                                  <  2:2490951/150‑1 (MQ=255)

                       cAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATGTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGAt                                                                                                                                <  1:3678104/150‑1 (MQ=255)

                         gcgcAATGATTCCTTCTGTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGAATAAAGCTG‑TTTGCCGATCCTTTCATTATTTGTTCCAGTCTGTCAGGAATTGTTCGATTc                                                                                                                              <  1:2627169/147‑1 (MQ=255)

                            cAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCtt                                                                                                                           <  2:2246047/150‑1 (MQ=255)

                            cAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTACCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGCCATAAAGCTG‑TTTGCCACCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCtt                                                                                                                           <  1:3009292/150‑1 (MQ=255)

                             aaTGATTCCTTCTCTGTAAAGCGGCGTCAGCACCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCttt                                                                                                                          <  2:3533027/150‑1 (MQ=255)

                              aTGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTg                                                                                                                         <  1:47397/150‑1 (MQ=255)

                               tGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGGGGGAAACAAGCATAAAACTG‑TTTGTCGCCCCCTTCTTTATTTGTTTCCGTCTGGCCGGGATTGTTTGGTTCCttttt                                                                                                                        >  1:553909/1‑148 (MQ=255)

                               tGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGt                                                                                                                        <  2:3073208/150‑1 (MQ=255)

                                      ttCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGGGGGAAAAAGGGATAAAACAG‑TTTGCCGCCCCCTTCATTATTTTTTTCAGTCTGCCAGGGAATGTTTCGTTCCTTtgtttgttta                                                                                                                 >  2:2516898/1‑148 (MQ=255)

                                       tgtctGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAAc                                                                                                                <  2:3682141/148‑1 (MQ=255)

                                       tctcTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAAc                                                                                                                <  1:2899555/150‑1 (MQ=255)

                                       tctcTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAAc                                                                                                                <  2:54919/150‑1 (MQ=255)

                                       tctcTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCAGGGCCAACACCGAGAAAACGG‑TTTTCCGCCCCCCTCAATATTTGTTTCTGTCTTCCAGGAATAGTTCTATGTCTTTTTCTGtttgc                                                                                                                >  1:3855435/1‑147 (MQ=255)

                                            gTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGgtg                                                                                                           <  1:2814191/150‑1 (MQ=255)

                                                   ggcgTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTg                                                                                                    <  2:2394763/150‑1 (MQ=255)

                                                         aGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCCTTAATTGTTTCACTCTGCCAGGAATTTTTTGATTCCTTTGTCTGTTTACGGGGGTTTTGTGGGGGCa                                                                                              >  2:3651869/1‑150 (MQ=255)

                                                            aTCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATGAAACTG‑CTTGCCGCCCCCTTCATTTTTTGTTTCCATCCGCCAGGAAATGTTCGCGTCCTTTTTCTGTTAACCGGTGTTTTTTGTGGGCatga                                                                                           >  1:2214870/1‑150 (MQ=255)

                                                            aTCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGCAAACAGGGATAAAGCTG‑TTTGCCGCCCCTTTCATTTTTTGTTCCAGTCCGCCAGGAATTGTTCGATTCCCTTGTCTGTTAACGGGTGGTTAGTGTGGGGatga                                                                                           >  1:3423314/1‑150 (MQ=255)

                                                              cTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCatgaat                                                                                         <  2:1411070/150‑1 (MQ=255)

                                                              cTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCatgaat                                                                                         <  2:3603080/150‑1 (MQ=255)

                                                               tGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAAAAGGCATAAAGCTG‑TTTGTCCCCCCTTTCATTATTTGTTTCACTCTGCCAGGAAATGTTTGGTTTCTTTTTCTGTTTAAGGGTGGTTAGTGGGCGCatgaatg                                                                                        >  1:3485478/1‑150 (MQ=255)

                                                               tGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGAGGCAAACAGGCATAAAACTG‑TTTGGCGCCCCCTTCATTATTTGTTTCAGTCTGCCAGCAAATGTTTGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCatgaatg                                                                                        >  1:3954941/1‑150 (MQ=255)

                                                                     tCCGGTGTATCAGGCGGGTGAAATACAAAGCCCGCGCCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTc                                                                                  <  1:207577/150‑1 (MQ=255)

                                                                       cGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAACTG‑TTTGCCGCCCCCTTCATTATTTTTTCCAGTCTGGCAGGGAATGTTCGGTTTCTTTTTCTGTTAACGGGTGTTTTGTGTGGGCATGAATGACTTTctg                                                                                >  2:4724301/1‑148 (MQ=255)

                                                                        gCTGTATCAGGTCGGTGAAATACAAAGTCCGCGGCAAACAGGCATAAACCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGc                                                                               <  1:3442828/150‑1 (MQ=255)

                                                                        gCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGc                                                                               <  2:3183187/150‑1 (MQ=255)

                                                                        gCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGc                                                                               <  2:2115058/150‑1 (MQ=255)

                                                                         cTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCg                                                                              <  2:823110/150‑1 (MQ=255)

                                                                            tATCAGGTCGGTGAAATACAAAGGCCGCGGGAAACAGGCATAAAGCTG‑TTTTCCGCCCCCTTCATTTATTTTTCCCGTCTGCCAAGGATTGTTCGATTCCCTTGTCTGTTTTAGGGTGTTTAGTGAGCGCATGAATGGCTTTCAGCGGcc                                                                           >  1:1055512/1‑149 (MQ=255)

                                                                             aTCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCAt                                                                          <  2:2801/150‑1 (MQ=255)

                                                                              tCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCACCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCAtt                                                                         <  1:1326588/150‑1 (MQ=255)

                                                                               cAGGTCGGTGAAATACAAAGGCCGCGGCAAAAAGGCAAAAAACTG‑TTTGCCGCCCCCTTCATTAATTGTTTCAATCTGCCCGGGAATGTTCGGTTCCTTTTTCTGTTTACGGGTGGTTTGGGTGGGCATGGATGACTTTTAGCGGCAAtg                                                                        >  2:4429722/1‑150 (MQ=255)

                                                                                aGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATtgt                                                                       <  2:3920970/150‑1 (MQ=255)

                                                                                 ggTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATtgtg                                                                      <  2:4373316/150‑1 (MQ=255)

                                                                                      gTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGat                                                                 <  2:3957755/150‑1 (MQ=255)

                                                                                       tGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGata                                                                <  1:1715412/150‑1 (MQ=255)

                                                                                        gAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGatat                                                               <  1:3790175/150‑1 (MQ=255)

                                                                                           aTACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAg                                                            <  1:790682/150‑1 (MQ=255)

                                                                                            tACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGc                                                           <  2:955642/150‑1 (MQ=255)

                                                                                             aCAAAGGCCGCGGCAAACAGGGATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGGGTTTAGGGGGGGCATGAAAGACTTTCTGCGGGATTGTGCCGATATGAggc                                                          >  2:1829439/1‑148 (MQ=255)

                                                                                             aCAAAGCCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCTCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGcc                                                          <  1:4495240/150‑1 (MQ=255)

                                                                                              cAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGccc                                                         <  2:431894/150‑1 (MQ=255)

                                                                                               aaaGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGcccc                                                        <  2:1552238/150‑1 (MQ=255)

                                                                                                  ggCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCCCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCctct                                                     <  1:2275662/150‑1 (MQ=255)

                                                                                                  ggCCGCGGCAAACAGCCATAAAGCTG‑TTTGCCACCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCctct                                                     <  1:3120682/150‑1 (MQ=255)

                                                                                                  gcccGCGGCAAACAGGCATAAAGCTG‑TTTGCCGTCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCctct                                                     <  1:1950215/148‑1 (MQ=255)

                                                                                                    ccGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTaa                                                   <  2:1729065/150‑1 (MQ=255)

                                                                                                    ccGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTaa                                                   >  2:4009900/1‑150 (MQ=255)

                                                                                                     cccgGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTGTCATTATTCGTTCAAGACTTTCAGGAGTGTCTCGATTCCGTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAag                                                  >  1:1877192/3‑150 (MQ=255)

                                                                                                      gcgGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAaga                                                 >  2:475084/1‑150 (MQ=255)

                                                                                                             aCAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCtt                                          >  1:1585776/1‑150 (MQ=255)

                                                                                                             aCAGGCATAAAGCTG‑TTTCCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCtt                                          <  1:1452761/150‑1 (MQ=255)

                                                                                                                ggCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTg                                       >  1:680696/1‑150 (MQ=255)

                                                                                                                    tAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCAc                                   <  2:3341048/150‑1 (MQ=255)

                                                                                                                      aaGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGt                                 >  1:3695568/1‑150 (MQ=255)

                                                                                                                       aGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGtg                                <  1:138155/150‑1 (MQ=255)

                                                                                                                         cTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGtgct                              <  2:189512/150‑1 (MQ=255)

                                                                                                                         cTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGtgct                              <  2:1268699/150‑1 (MQ=255)

                                                                                                                         cTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGtgct                              >  1:480603/1‑150 (MQ=255)

                                                                                                                          tG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGANCCCCTCTAAGAGCAGCTTCTGTCACGtgctg                             >  1:1626285/1‑150 (MQ=255)

                                                                                                                           g‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGtgctgc                            >  1:1669676/1‑150 (MQ=255)

                                                                                                                                gcctccCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGAt                        <  1:632424/150‑1 (MQ=255)

                                                                                                                                gccgccCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGTCTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGAt                        >  2:1576468/1‑150 (MQ=255)

                                                                                                                                gccgccCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGAt                        >  1:855649/1‑150 (MQ=255)

                                                                                                                                 cctccccTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTTGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATg                       <  2:4568505/147‑1 (MQ=255)

                                                                                                                                   gccCCTTTCATTATTTGTTCCAGTCTGCCAGGACTTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGc                     <  2:462889/150‑1 (MQ=255)

                                                                                                                                    ccccTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCg                    >  2:4960006/1‑150 (MQ=255)

                                                                                                                                      ccTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGa                  >  1:2417033/1‑150 (MQ=255)

                                                                                                                                         ttCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTg               <  2:2780476/150‑1 (MQ=255)

                                                                                                                                         ttCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTg               >  2:1502747/1‑150 (MQ=255)

                                                                                                                                          tCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGa              >  1:3542803/1‑150 (MQ=255)

                                                                                                                                           cATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGAc             <  2:65700/150‑1 (MQ=255)

                                                                                                                                           cATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGAc             <  1:1062759/150‑1 (MQ=255)

                                                                                                                                           cATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGAc             >  1:3199004/1‑150 (MQ=255)

                                                                                                                                              tattTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCt          >  2:754784/1‑150 (MQ=255)

                                                                                                                                                tttGTTCCAGTCTGCCACGAATTGTTCGATTACTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGAATTGTGCTGATATGAGCGCCTCTCAGAGCAGCTTCTGTCAGGTGCTGCGGATGGCGGATTGACGCTGc        >  1:1167647/1‑150 (MQ=255)

                                                                                                                                                  tGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCaa      <  1:2169533/150‑1 (MQ=255)

                                                                                                                                                  tGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCaa      >  1:1219356/1‑150 (MQ=255)

                                                                                                                                                   gTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCAAt     >  1:296011/1‑150 (MQ=255)

                                                                                                                                                   gTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCAAt     <  1:4915675/150‑1 (MQ=255)

                                                                                                                                                    ttCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCTTTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCAATg    <  2:1128223/150‑1 (MQ=255)

                                                                                                                                                    ttCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCAATg    <  1:505199/150‑1 (MQ=255)

                                                                                                                                                     tCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGTCGCTGCAATGa   >  1:4420591/1‑150 (MQ=255)

                                                                                                                                                     tCCACTCTGCCAGGAATTGTTCGATTCCTTTGTCTATTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCAATGa   <  1:4040965/150‑1 (MQ=255)

                                                                                                                                                      ccAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCAATGAt  <  1:2529679/150‑1 (MQ=255)

                                                                                                                                                      ccAGTCTGCCAGAAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCACTCTAAGAGCAGCTACTGTCACGTGCTCCAGATGGAGGATTGACGCTGCAATGAt  >  2:4097651/1‑150 (MQ=255)

CTTCATTCCGATATCAGCTTCTGCAGCCCAATGATTCCTTCTCTGTAAAGCGGCGTCAGCATCTGAAGATCCGCTGTATCAGGTCGGTGAAATACAAAGGCCGCGGCAAACAGGCATAAAGCTG‑TTTGCCGCCCCTTTCATTATTTGTTCCAGTCTGCCAGGAATTGTTCGATTCCTTTGTCTGTTAACGGGTGTTTAGTGAGCGCATGAATGACTTTCAGCGGCATTGTGCCGATATGAGCCCCTCTAAGAGCAGCTTCTGTCACGTGCTGCGGATGGCGGATTGACGCTGCAATGAT  >  BS168_reference_genome/3807614‑3807912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`