BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,922	C→T	27.3%	intergenic (–/‑1056)	– / → eryR	–//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,922	0	C	T	27.3%	33491.7 / inf	16115	intergenic (–/‑1056)	–/eryR	–//vntifkey=4
Reads supporting (aligned to +/- strand): ref base C (6654/5018); new base T (2576/1817); total (9266/6849)
Fisher's exact test for biased strand distribution p-value = 6.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-09

Only 100 of 16303 total aligned reads displayed.

TATATTTTAGTAAACTCCCCGTTAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCT  >  Exported/1773‑2071

      ttAGTAAACTCCCCGTTAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAg                                                                                                                                                 >  2:46160/1‑150 (MQ=255)

        aGTAAACTCCCCGTTAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGGTTTTCGCAGGACCCTTTTTTAGATCGAACATAATTGATAAGAACAAATAAACGCTTGGGTCCAACTTTATAGTAATTAAta                                                                                                                                               >  1:676289/1‑150 (MQ=255)

        aGTAAACTCCCCGTTAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGta                                                                                                                                               >  2:363300/1‑150 (MQ=255)

        aGTAAACTCCCCGTTAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGta                                                                                                                                               <  2:3970401/150‑1 (MQ=255)

         gTAAACTCCCCGTTAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGtat                                                                                                                                              >  2:1838835/1‑150 (MQ=255)

              cTCCCCGTTAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGt                                                                                                                                         <  2:2635753/150‑1 (MQ=255)

                      tAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTaaa                                                                                                                                 >  2:4480421/1‑150 (MQ=255)

                      tAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTaaa                                                                                                                                 >  1:2804933/1‑150 (MQ=255)

                      tAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTaag                                                                                                                                 >  2:4973205/1‑149 (MQ=255)

                        gCTTAACAGGTCTTTGTAAGCAATTAAACGACCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATACCCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAAt                                                                                                                               <  2:235102/150‑1 (MQ=255)

                         cTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATc                                                                                                                              >  2:1315228/1‑150 (MQ=255)

                         cTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGGTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCCTGGGACCAACTTTATAGCAATTAGTATATGGTCATTTAAAATc                                                                                                                              >  1:3041277/1‑150 (MQ=255)

                          ttAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTAAATGGTCATTTAAAATCt                                                                                                                             <  2:4761649/150‑1 (MQ=255)

                              cAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTGGTATATGGTCATTTAAAATCTTTAc                                                                                                                         <  2:3076134/150‑1 (MQ=255)

                              cAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTAc                                                                                                                         <  2:4112324/150‑1 (MQ=255)

                               aGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTAcc                                                                                                                        >  2:4901904/1‑150 (MQ=255)

                                  tCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAAt                                                                                                                     >  2:2137096/1‑150 (MQ=255)

                                   cTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAAtt                                                                                                                    <  2:603433/150‑1 (MQ=255)

                                        tAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACg                                                                                                               >  2:1916786/1‑150 (MQ=255)

                                          aGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCt                                                                                                             >  2:1164139/1‑150 (MQ=255)

                                           gCAATTAAACTTCCACTATTCAATCGTCTTTGGATTTTCCCAGGACCGTTTTTTAGAACGAACATAGTTGATAAGAACAAATAACCGCTTGGGTACAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTa                                                                                                            <  2:522901/150‑1 (MQ=255)

                                            cAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTAt                                                                                                           <  2:758064/150‑1 (MQ=255)

                                               ttAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAg                                                                                                        >  1:2213072/1‑150 (MQ=255)

                                               ttAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAg                                                                                                        >  2:1615939/1‑150 (MQ=255)

                                                     gTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCtt                                                                                                  >  1:4353467/1‑150 (MQ=255)

                                                       ccACTATTCAATCGTCTTTGGATTTTCGCAGCACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAAACGCTTGGGTCCAAATTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAAGGCTATTAGGTTCTTTa                                                                                                >  1:1000670/1‑150 (MQ=255)

                                                        cACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAg                                                                                               >  1:3736598/1‑150 (MQ=255)

                                                             ttCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGAttt                                                                                          >  2:3214536/1‑150 (MQ=255)

                                                             ttCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGAttt                                                                                          >  2:2564/1‑150 (MQ=255)

                                                              tCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGAtttt                                                                                         >  2:2935745/1‑150 (MQ=255)

                                                              tCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGAtttt                                                                                         <  2:4541172/150‑1 (MQ=255)

                                                                aaTCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGc                                                                                       >  1:2445746/1‑150 (MQ=255)

                                                                 aTCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGcc                                                                                      <  2:4265584/150‑1 (MQ=255)

                                                                     tCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGAc                                                                                  >  1:1232410/1‑150 (MQ=255)

                                                                     tCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGAc                                                                                  >  2:832891/1‑150 (MQ=255)

                                                                      cTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACa                                                                                 >  2:681463/1‑150 (MQ=255)

                                                                       tttGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACAt                                                                                <  2:1982040‑M1/150‑2 (MQ=255)

                                                                         tGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACAtag                                                                              >  1:4704030‑M1/1‑147 (MQ=255)

                                                                         tGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACAtag                                                                              >  1:1959621‑M1/1‑147 (MQ=255)

                                                                          ggATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACAtagt                                                                             >  2:1010621‑M1/1‑146 (MQ=255)

                                                                          ggATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACAtagt                                                                             >  2:3145321‑M1/1‑146 (MQ=255)

                                                                          ggATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACAtagt                                                                             <  2:1689901‑M1/150‑5 (MQ=255)

                                                                            aTTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCg                                                                           <  2:2211647/150‑1 (MQ=255)

                                                                            aTTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCg                                                                           >  1:4589137/1‑150 (MQ=255)

                                                                              tttCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTTTAGCAATTGGTATATGGTCATTTAAAATCTTTACCCATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCggg                                                                         <  2:3696568/150‑1 (MQ=255)

                                                                               ttCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCgggg                                                                        >  1:3517325/1‑150 (MQ=255)

                                                                                tCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGgt                                                                       <  2:1541843/150‑1 (MQ=255)

                                                                                tCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGgt                                                                       <  2:4186909/150‑1 (MQ=255)

                                                                                 cGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGgtg                                                                      <  2:3826846/150‑1 (MQ=255)

                                                                                    agcaCCGTTTTTTAGATCGAACATAGTTGATTAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTCAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTc                                                                   >  2:3007595/4‑150 (MQ=255)

                                                                                     ggACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGCTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGTCATAGTCGGGGTGTTCa                                                                  >  1:3275621/1‑150 (MQ=255)

                                                                                       aCCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAAGCACTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTACGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAAc                                                                >  1:2089350/1‑150 (MQ=255)

                                                                                        ccGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACg                                                               >  2:389857/1‑150 (MQ=255)

                                                                                          gTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGat                                                             <  2:2425856/150‑1 (MQ=255)

                                                                                            tttttAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGatat                                                           <  2:2873633/150‑1 (MQ=255)

                                                                                                tAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCttt                                                       <  2:4831699‑M2/149‑1 (MQ=255)

                                                                                                 aGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACA‑AGTCGGGGTGTTCAACGATATCTTTTa                                                     >  1:785805/1‑150 (MQ=255)

                                                                                                  gATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTTGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTa                                                     >  1:315728/1‑150 (MQ=255)

                                                                                                  gATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTa                                                     <  2:3431272/150‑1 (MQ=255)

                                                                                                     cGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCGATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTAtgt                                                  >  1:905890/1‑150 (MQ=255)

                                                                                                      gAACATAGTTGGTAAGAACAAATACCCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTAtgtg                                                 <  2:4040442/150‑1 (MQ=255)

                                                                                                      gAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTAtgtg                                                 >  2:917350/1‑150 (MQ=255)

                                                                                                      gAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTAtgtg                                                 >  1:1360746/1‑150 (MQ=255)

                                                                                                        aCATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCg                                               <  2:2272671/150‑1 (MQ=255)

                                                                                                          aTAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGAt                                             >  1:3391984/1‑150 (MQ=255)

                                                                                                          aTAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGAt                                             <  2:3762307/150‑1 (MQ=255)

                                                                                                              ttGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAat                                         <  1:4945155/150‑1 (MQ=255)

                                                                                                               tGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAata                                        <  2:4404333/150‑1 (MQ=255)

                                                                                                                  tAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATAttt                                     >  1:4461975/1‑150 (MQ=255)

                                                                                                                  tAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGACCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATAttt                                     >  1:41127/1‑150 (MQ=255)

                                                                                                                    aGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATAttttt                                   <  2:2051595/150‑1 (MQ=255)

                                                                                                                    aGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATAttttt                                   >  1:1731134/1‑150 (MQ=255)

                                                                                                                     gAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTc                                  <  2:2715160/150‑1 (MQ=255)

                                                                                                                     gAACAAATAACCGCTTGAATCCAACTTTATAGCAATTAGTATATGGTCAGTTAAAATCTTTACCAATTCAACCCTATTAGGTTCTTTAGAATATTGCCCGACATTGTCTGGGGGTTCAACCATATCTTTTATGTGCGATGAATATTTTTc                                  >  1:231766/1‑150 (MQ=255)

                                                                                                                      aaCAAATAACCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCa                                 >  1:4232203/1‑150 (MQ=255)

                                                                                                                       aCAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCAt                                <  2:1245544/150‑1 (MQ=255)

                                                                                                                       aCAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCAt                                >  1:2691395/1‑150 (MQ=255)

                                                                                                                        cAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATa                               >  2:3565875/1‑150 (MQ=255)

                                                                                                                         aaaTAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATaa                              >  2:1098520/1‑150 (MQ=255)

                                                                                                                          aaTAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCGATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATaaa                             >  1:2924984/1‑150 (MQ=255)

                                                                                                                            tAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATa                           >  1:1853600/1‑150 (MQ=255)

                                                                                                                             aaCCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATAc                          >  1:4820136/1‑150 (MQ=255)

                                                                                                                             aaCCGCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATAc                          >  2:2496082/1‑150 (MQ=255)

                                                                                                                              agcGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTACGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATAcc                         <  1:545590/148‑1 (MQ=255)

                                                                                                                                cGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAG                       <  2:4689827‑M1/150‑1 (MQ=255)

                                                                                                                                 gCTTGGGTCCAACTTTATAGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAgg                      <  2:140156/150‑1 (MQ=255)

                                                                                                                                 gCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAgg                      <  2:1767076/150‑1 (MQ=255)

                                                                                                                                  cTTGCGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGa                     >  1:2573762/1‑150 (MQ=255)

                                                                                                                                       gTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGAtgttg                <  1:1125717/150‑1 (MQ=255)

                                                                                                                                       gTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGAtgttg                >  2:1474813/1‑150 (MQ=255)

                                                                                                                                         ccAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATgttgtt              >  2:4330399/1‑150 (MQ=255)

                                                                                                                                            aCTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCTCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTGATAAATACCAGGATGTTGTTTCt           <  1:3849147/150‑1 (MQ=255)

                                                                                                                                              tttATAACAATTAGTATATGGACATTTAAAATTTGTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCACGACATAGTCTGAGTGTTCAAAGATATCTTTTATGTGCTATGAATATTTTTCATAAATACCAGGATGTTGTTTCttt         >  1:3632095/1‑150 (MQ=255)

                                                                                                                                               ttATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTa        <  1:1480221/150‑1 (MQ=255)

                                                                                                                                                tataGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTAc       <  2:3493175/150‑1 (MQ=255)

                                                                                                                                                tataGCAATTGGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTAc       <  2:3291697/150‑1 (MQ=255)

                                                                                                                                                 ataGCTATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACg      <  2:2790244/150‑1 (MQ=255)

                                                                                                                                                 ataGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACg      <  1:160719/150‑1 (MQ=255)

                                                                                                                                                  taGTAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGt     >  1:3991189/1‑150 (MQ=255)

                                                                                                                                                    gCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGc   <  2:3357057/150‑1 (MQ=255)

TATATTTTAGTAAACTCCCCGTTAGCTTAACAGGTCTTTGTAAGCAATTAAACGTCCACTATTCAATCGTCTTTGGATTTTCGCAGGACCGTTTTTTAGATCGAACATAGTTGATAAGAACAAATAACCGCTTGGGTCCAACTTTATAGCAATTAGTATATGGTCATTTAAAATCTTTACCAATTCAACGCTATTAGGTTCTTTAGGATTTTGCCCGACATAGTCGGGGTGTTCAACGATATCTTTTATGTGCGATGAATATTTTTCATAAATACCAGGATGTTGTTTCTTTACGTGCT  >  Exported/1773‑2071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`