BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	2,480,654	Δ1 bp	100%	intergenic (+29/+96)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,480,654	0	T	.	99.2%	463.9 / ‑3.1	118	intergenic (+29/+96)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (49/68); minor base G (0/1); total (49/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 118 total aligned reads displayed.

CAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGT  >  BS168_reference_genome/2480525‑2480787

cacacaaaACCTTATCTACAACCTACGCATCCGGCTCACTGGGACCGAAAGACTCTGCTGAGCTTTTGGGGAAAGACGGCTTACACTGGTGGAACATAAAATAAGACGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTc                                                                                                                             <  5:80386/136‑1 (MQ=255)

caaacaaaACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTc                                                                                                                             >  3:118054/1‑139 (MQ=255)

  aacaaaCCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGCTCTTTTGGGGAAAGACGGCTTACACTGGTGGAACATATAAAAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCgg                                                                                                                           <  2:143040/139‑1 (MQ=255)

  aacaaaACCTTATCTCCTAACTCCGAAACCGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGGGAAAGACGGCTTACACTGGTGGAACATATAATAAGAACAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCgg                                                                                                                           <  3:18505/139‑1 (MQ=255)

  aacaaaACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCgg                                                                                                                           <  8:243335/139‑1 (MQ=255)

     aaaaCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCggctt                                                                                                                        >  8:294032/1‑136 (MQ=255)

       aaCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑Ctttt                                                                                                                     >  2:4884/1‑139 (MQ=255)

            tATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTAct                                                                                                                >  7:392176/1‑138 (MQ=255)

            tATCACCTAAATACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                <  5:396426/139‑1 (MQ=255)

             aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                <  2:334310/138‑1 (MQ=255)

             aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                <  7:106562/138‑1 (MQ=255)

             aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                               >  2:5191/1‑139 (MQ=255)

                  cTAACTACGAATCAGGATAAATGGGACCTAAAGAATCTGATGATTTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                          <  8:392176/139‑1 (MQ=255)

                  cTAAATACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTg                                                                                                             <  1:196941/136‑1 (MQ=255)

                   tAACTACGAATCATGCTCATTGGGACCGATAGTATCTGATTATCTTTTGGTGAAAGACGGCTTACACTTGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTT‑TGAAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                                         <  6:331293/139‑1 (MQ=255)

                   tAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                          >  7:334211/1‑138 (MQ=255)

                   tAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                          >  1:21357/1‑138 (MQ=255)

                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATTAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                        <  4:130296/139‑1 (MQ=255)

                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCttgtctc                                                                                                        >  3:309150/1‑133 (MQ=255)

                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                        <  1:298164/139‑1 (MQ=255)

                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTAAACTGGGGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                        <  4:276878/139‑1 (MQ=255)

                     aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                        <  7:220104/138‑1 (MQ=255)

                     aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                                         <  6:151406/137‑1 (MQ=255)

                         cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                        <  4:425289/134‑1 (MQ=255)

                         cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                        >  3:425289/1‑134 (MQ=255)

                            aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                >  4:94112/1‑139 (MQ=255)

                            aTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                                >  3:338829/1‑139 (MQ=255)

                               aGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                          >  6:386727/1‑126 (MQ=255)

                                ggCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGGGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                            <  4:403938/139‑1 (MQ=255)

                                ggCTCAATGGCACCCAAAGAATGTGCTGATCTTTTGGTGAAAGACGGCTTACTCTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                            <  3:267961/139‑1 (MQ=255)

                                ggCACAATGGGACCGAAAGCATCTTCTCATCTTTAGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                            <  7:361822/139‑1 (MQ=255)

                                 gCTCAATGGGACCGAAAGAATCTTATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                           <  6:278104/139‑1 (MQ=255)

                                 gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                           >  3:256336/1‑139 (MQ=255)

                                 gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                            <  7:237036/138‑1 (MQ=255)

                                      aTGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCtt                                                                                      <  3:392652/139‑1 (MQ=255)

                                      aTGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCt                                                                                       >  6:278399/1‑138 (MQ=255)

                                           aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCAtttt                                                                                                                              <  8:330260/95‑1 (MQ=255)

                                           aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                                 >  1:13648/1‑139 (MQ=255)

                                           aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                                 >  7:104158/1‑139 (MQ=255)

                                                    agcTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                        <  1:289808/137‑1 (MQ=255)

                                                    aTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                        <  7:294032/139‑1 (MQ=255)

                                                    aTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                        >  1:361598/1‑139 (MQ=255)

                                                     tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                        >  1:407807/1‑138 (MQ=255)

                                                     tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTGTACGCTGAACAAAGGTATAACAATCTGCTTAATAACAAAAcccc                                                                        >  7:184477/1‑138 (MQ=255)

                                                         atgttgttttGGTGAAAGACGGCTTAAACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                      <  6:140505/130‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                      <  4:144998/136‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                      <  8:104158/136‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                      <  1:259080/136‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                      <  2:100168/136‑1 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                                   >  6:305411/1‑139 (MQ=255)

                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                                   >  3:416514/1‑139 (MQ=255)

                                                         atgatCGTTTGGGTAAAGACGGCTTACACTGGTGGAACAAATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                      <  6:140508/136‑1 (MQ=255)

                                                          tgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                                   <  5:304173/138‑1 (MQ=255)

                                                             tCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCtt                                                                >  5:52030/1‑138 (MQ=255)

                                                               ttttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                >  5:133672/1‑88 (MQ=255)

                                                               ttttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                <  6:133672/88‑1 (MQ=255)

                                                               ttttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCtt                                                                >  3:343534/1‑136 (MQ=255)

                                                                 ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTaa                                                              >  7:158251/1‑136 (MQ=255)

                                                                 ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTaa                                                              <  5:302291/136‑1 (MQ=255)

                                                                 ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                             <  4:249350/137‑1 (MQ=255)

                                                                       aaaGACGTCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCg                                                      <  4:343534/138‑1 (MQ=255)

                                                                       aaaGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCg                                                      <  6:74723/138‑1 (MQ=255)

                                                                               cTTACACTTGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCt                                             <  8:103738/139‑1 (MQ=255)

                                                                                  acacTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTa                                            <  4:416514/137‑1 (MQ=255)

                                                                                     cTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGAt                                       <  5:32027/139‑1 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                        >  2:359929/1‑103 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                        <  1:359929/103‑1 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                    >  3:287015/1‑139 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                    >  1:19283/1‑139 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                    >  7:145781/1‑139 (MQ=255)

                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                    >  3:134794/1‑139 (MQ=255)

                                                                                         tggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACt                                   >  2:390869/1‑139 (MQ=255)

                                                                                             aCATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAt                               <  2:407807/139‑1 (MQ=255)

                                                                                                tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                      <  1:98850/97‑1 (MQ=255)

                                                                                                tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                                 >  4:414485/1‑118 (MQ=255)

                                                                                                tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                                 <  3:414485/118‑1 (MQ=255)

                                                                                                tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAt                               <  5:6939/136‑1 (MQ=255)

                                                                                                tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAt                               >  6:6939/1‑136 (MQ=255)

                                                                                                tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCa                            <  5:245022/139‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGttt                                               >  5:93210/1‑118 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGttt                                               <  6:93210/118‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                          <  6:352172/139‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                          <  4:287015/139‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                          <  4:155428/139‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAt                           >  8:146929/1‑138 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTAAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                          <  6:39740/139‑1 (MQ=255)

                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTAAGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                          <  2:197363/139‑1 (MQ=255)

                                                                                                   aataaGAACAAAACAGCCGAAAAGGTTTAC‑AAGCATTTTAGG‑CTTTTTTACGCTGAACAAACGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTc                         <  1:279450/139‑1 (MQ=255)

                                                                                                       agaagaAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTa                     <  3:94112/139‑1 (MQ=255)

                                                                                                        gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTAt                    >  5:260185/1‑139 (MQ=255)

                                                                                                        gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTAt                    >  4:309351/1‑139 (MQ=255)

                                                                                                        gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTAt                    >  4:211153/1‑139 (MQ=255)

                                                                                                         aagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTTCGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCg                                                      >  4:5438/1‑104 (MQ=255)

                                                                                                         aagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCg                                                      <  3:5438/104‑1 (MQ=255)

                                                                                                           gaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGATGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTGTATTGATTACTTCATCCATTCAGTATgg                  >  3:144987/1‑138 (MQ=255)

                                                                                                                aaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATg            <  2:19283/139‑1 (MQ=255)

                                                                                                                aaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATg            <  4:338829/139‑1 (MQ=255)

                                                                                                                  gCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTTCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAACCATTCAGTATGGAAGATGcc          <  1:4884/139‑1 (MQ=255)

                                                                                                                   ccGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCct         >  3:177994/1‑139 (MQ=255)

                                                                                                                          tgTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGt  <  8:334211/138‑1 (MQ=255)

CAAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGT  >  BS168_reference_genome/2480525‑2480787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`