BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,741	G→T	11.2%	intergenic (+26/‑130)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,741	0	G	T	11.2%	18875.3 / inf	6634	intergenic (+26/‑130)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (201/5531); new base T (780/82); total (1012/5622)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6821 total aligned reads displayed.

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  Exported/3603‑3882

   aGTTTCATCAAGAAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGa                                                                                                                                             <  4:101141/138‑1 (MQ=255)

   aGTTTCAGAAAGCAAAGAAACACGCCAAAGAAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGATATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGa                                                                                                                                             <  7:244794/138‑1 (MQ=255)

       tcatcaGGCAATGAAACACGACAAAGTATACAATTTAAGTACCATTACTTATGAGCAAGATTTGTGTATTTTTAATAGGTATCTATTATTTTACTGGAGGAAATTATTCTATGAGTCGCCGTTTTTAAAATTgagtttt                                                                                                                                         >  4:163013/1‑133 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTa                                                                                                                                       <  6:353898/138‑1 (MQ=255)

         atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTa                                                                                                                                       <  4:293673/138‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  3:307187/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  3:141403/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  5:211751/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  8:362519/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  5:427494/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  1:157084/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  4:195729/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  1:95305/139‑1 (MQ=255)

          tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTaca                                                                                                                                     <  5:400159/139‑1 (MQ=255)

           caAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTacac                                                                                                                                    <  1:39519/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTacac                                                                                                                                    <  7:268366/138‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  3:352364/139‑1 (MQ=255)

            aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                   <  5:241288/139‑1 (MQ=255)

               cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTa                                                                                                                                <  3:72101/139‑1 (MQ=255)

                 aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                              <  7:163267/139‑1 (MQ=255)

                  tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTTAATTTGGAAAGTTTCACCTTaat                                                                                                                              >  4:322035/1‑136 (MQ=255)

                  tGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CGTTTTTTAATTTTTAAATTTACACggtgat                                                                                                                              >  8:233425/1‑133 (MQ=255)

                    aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTaa                                                                                                                            <  1:233429/138‑1 (MQ=255)

                        acGCCAAACTAAACAATTTAACTACCATTACATATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                                       <  2:321049/139‑1 (MQ=255)

                         cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                      <  7:348592/139‑1 (MQ=255)

                           ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                                    <  1:27025/139‑1 (MQ=255)

                           ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                                    <  3:168470/139‑1 (MQ=255)

                           ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATAGTCTATTTTTAATAGGTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                                    <  4:4180/139‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  4:390385/139‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  8:70512/139‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAAATCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  3:384100/139‑1 (MQ=255)

                                 tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAAAAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  6:185731/139‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  2:205315/138‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  2:128005/138‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                              <  8:112533/138‑1 (MQ=255)

                                  aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACAAGTTACTACAGTGAATGGAGATa                                                                                                              <  6:394291/138‑1 (MQ=255)

                                   aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                            <  3:108625/139‑1 (MQ=255)

                                   aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                            <  8:261498/139‑1 (MQ=255)

                                     aaaTTTAAGTAACATTACTTATGAGAAAGTATTGTATATTTTTAATAGTAATCTATTATTTAACGGTAGGAAATAATTCTATGAGTCG‑CTTATTTAAATTTGTAAATTTACACGTTACTAAATGTAATGGAGATTAatt                                                                                                          <  2:44821/138‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:33362/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:124506/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  5:186278/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  7:191178/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  7:415135/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  1:301500/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                       <  1:191418/139‑1 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CGTTTTTTAATTTTTTAAGTTTCAAGTTAATAAATGGTATTGAGATAAattatt                                                                                                       >  2:28069/1‑139 (MQ=255)

                                        tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CGTGTTTTTATTTTGAAAGATTAAAGTTAAGAAAAGGGATGGAGATAAattatt                                                                                                       >  6:126176/1‑139 (MQ=255)

                                             gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                  <  7:382615/139‑1 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGTAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                <  8:330497/138‑1 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                <  7:54597/138‑1 (MQ=255)

                                                ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                <  1:365096/138‑1 (MQ=255)

                                                  aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CGTTTTTTAATTTTTAAAATTTAAAAATAATAAAAGGAATTGATAAAAAATATtatataaaaa                                                                                              >  4:63822/1‑134 (MQ=255)

                                                      cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTATATTTGGAAAGTTACACGTTACTAACGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                         <  7:111313/139‑1 (MQ=255)

                                                       ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                        <  4:23007/139‑1 (MQ=255)

                                                          tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAg                                                                                      <  4:259030/138‑1 (MQ=255)

                                                              cAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CGTTTTTAAATTTTGTAATTTTCAAGTTTCGAAAGGGTATGGTGATTAATTATTTTATTTACTACTGACAGGTTc                                                                                  >  8:296460/1‑138 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  1:339559/139‑1 (MQ=255)

                                                               aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                <  8:394298/139‑1 (MQ=255)

                                                                 gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                              <  5:94102/139‑1 (MQ=255)

                                                                  tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  3:328747/139‑1 (MQ=255)

                                                                  tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  6:249007/139‑1 (MQ=255)

                                                                   aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGATACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                             <  5:100155/138‑1 (MQ=255)

                                                                        cTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                        <  5:158373/138‑1 (MQ=255)

                                                                            ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CGTTTTTATAATATGAAAGTTTCACGATACATAAGTGATAGGTCCTAAATTAATATATTTAAAAATGACAGCTACCAAGAAGATAAagac                                                                   >  8:138237/1‑138 (MQ=255)

                                                                             tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                   <  1:100526/138‑1 (MQ=255)

                                                                                 aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTcc                                                               <  1:68433/138‑1 (MQ=255)

                                                                                 aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CGTTTTTTTATTTTTTAAAATAGACCGTTGTAAAGGGGAAGGTGTTAAATTTTTTGATATACTAATGACCGCGTCCAAGAAGCTTAATAGGTccc                                                              >  2:251000/1‑139 (MQ=255)

                                                                                     ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTTAATTTTGTAAGTTTCACGTTTGTTAAGGGAATGGTGTTAAATTTTTTTATTTAATACTGTCAGCTTGCAAGAAGCTTAAGAGGTCCCGAgc                                                          >  7:217487/1‑139 (MQ=255)

                                                                                     ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTTGAAATTTAAACGTTTATAAAGTGTATGGAGATAAAATTTTTTATTTAATACAGAAAGCTTGCAAGAAGCTAAAGAGGTcccgatc                                                          >  6:78136/1‑135 (MQ=255)

                                                                                     ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CGTTTTTTAATTTTGAAATTTTCACGTTTATAAAGGGAATGGGGATAAATTTTTTTATTTTCTAAGGACCGCGTCCCAGAAGATAAAGAGGTCCCGAgc                                                          >  8:200448/1‑139 (MQ=255)

                                                                                      tATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CGGTTTTTAATTTGGATAGTTTAACGTTAGTTAAGGGGATGGAGATAAATTTTTTTATTTAATACTGACAGCGTCCAACCACCTAAAGAGGTCCCGAgcg                                                         >  4:352102/1‑139 (MQ=255)

                                                                                            ttattTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTAc                                                    <  5:68021/138‑1 (MQ=255)

                                                                                              attTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                  <  6:288421/138‑1 (MQ=255)

                                                                                                      ggaggaAATAATTCTATGAGTCG‑CGTTTTTTAAATAGGAAAATTTAAAGGTAAAAAATGTAAATGACATAAAAAAATATAAATAAAAATTACAACTTACAAGAAACTAAAGAGGTGCCAAGCGCCAAAGGGGAAatttt                                         >  6:154803/1‑137 (MQ=255)

                                                                                                         ggaAATAATTCTATGAGTCG‑CGTTTTTTAATTTTGTAAGTTTAACCGTTCTAAAGGGAATGGAGATAAATTATTATTTATACTACAGACAGCTTCCAACAAGCGAAAGAGGTCCCGAGCGCCTACGGGGAATTTggggt                                      >  6:319712/1‑135 (MQ=255)

                                                                                                            aataatTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                   <  3:255177‑M1/139‑8 (MQ=255)

                                                                                                            aataatTCTATGAGGCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                   <  5:268453‑M1/139‑8 (MQ=255)

                                                                                                                    tATGAGTCG‑CGTTTTTAAATTTTGAAAGTTTAACGGTAATAAATGGAATGGAGATAAATTATTATATATAATACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCGACGGGGGATTTTgggtacatttaaaaa                           >  2:75237‑M1/1‑124 (MQ=255)

                                                                                                                      tcaggcg‑ctttTTTAAATTTGGAAAGTTACACGTTACTTAAGCGAATCGAGATAAATTATAAGATATACTACTGACAGCTTCCAAGCAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  8:167200‑M1/134‑18 (MQ=255)

                                                                                                                      tGAGTCT‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  7:86613‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTACAGGGAATGGATATAAAATATTAGATATACAAATCACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  2:357343‑M1/139‑18 (MQ=255)

                                                                                                                      tGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                         <  4:132265‑M1/139‑18 (MQ=255)

                                                                                                                        aGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                        <  7:130321‑M1/138‑19 (MQ=255)

                                                                                                                        aGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                        <  5:366430‑M1/138‑19 (MQ=255)

                                                                                                                          gtagattttttaaattTTGAAAGTTAAAATTTACTAAATGTAATGGATATAAATTATTAGATATAATACTGACATCTTCAAAGAATATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                       <  1:133650‑M1/133‑20 (MQ=255)

                                                                                                                           cG‑CTTTTTTAAATTTGCAAAGTTACACTTAACTAAAGTTAATTGTGATAAATAATTAGATATACTACTGACAGCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                    <  2:161399‑M1/139‑23 (MQ=255)

                                                                                                                               ttttttAATTTTGTAAAGTTACACGTTAATAAAGGGAATTGAGATAAATTATTAGTTATACTTCTTACAGCTTCAAAGAATCTAAAGAGGTCCATAGCGCCTACGGGGAATTT‑‑‑‑‑GGTGTACATTGAAAAAGGAAgagtat            <  6:21306/139‑1 (MQ=255)

                                                                                                                               ttttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  8:427610‑M1/139‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  7:296085‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  3:197110‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  3:225414‑M1/137‑26 (MQ=255)

                                                                                                                                 ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                 <  3:407608‑M1/137‑26 (MQ=255)

                                                                                                                                  tttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga               <  1:42777‑M1/138‑28 (MQ=255)

                                                                                                                                     aattttggaAAGTTACACGTTACAAAAGGGAATGTATATAAATTATTAGATATAATACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta            <  3:280092‑M1/135‑31 (MQ=255)

                                                                                                                                     aaaTTTGGAAAGTTACACGTTACTAAAGGGATTGGAGATAAATTATTAGATATACTTCTGAGAGCTTAGAAGAAGCTATTGATGTCCCTAGGGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGATAAAGGAAgagtatgagta       <  6:428327/138‑1 (MQ=255)

                                                                                                                                     aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta            <  2:392289‑M1/138‑31 (MQ=255)

                                                                                                                                     aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta            <  3:47350‑M1/138‑31 (MQ=255)

                                                                                                                                      aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt          <  5:303294‑M1/139‑33 (MQ=255)

                                                                                                                                        tttGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattca        <  7:321251‑M1/139‑35 (MQ=255)

ATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCG‑CTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAA  >  Exported/3603‑3882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`