BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,749	A→T	6.3%	intergenic (+34/‑122)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,749	0	A	T	6.3%	18295.7 / 196.3	5836	intergenic (+34/‑122)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (545/4825); new base T (430/17); total (982/4854)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6473 total aligned reads displayed.

TCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACAT  >  Exported/3610‑3885

  atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTa                                                                                                                                          <  4:256881/138‑1 (MQ=255)

  atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTa                                                                                                                                          <  4:99681/138‑1 (MQ=255)

   tcacccAATGAACCACGCCAAAGTAAACAATTTAAGTACCAATACTTATGAGCAAGTATTGTCTATTTATAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                        <  3:70776/134‑1 (MQ=255)

   tcaAGCAATGTAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                        <  7:105307/139‑1 (MQ=255)

   tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                        <  3:278053/139‑1 (MQ=255)

   tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                        <  4:195729/139‑1 (MQ=255)

   tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                        <  6:72846/139‑1 (MQ=255)

   tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                        <  4:349099/139‑1 (MQ=255)

   tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                        <  5:124185/139‑1 (MQ=255)

   taaaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTaca                                                                                                                                        <  5:26543/137‑1 (MQ=255)

     aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTacac                                                                                                                                       <  8:357443/138‑1 (MQ=255)

     aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                      <  5:241288/139‑1 (MQ=255)

     aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                      <  3:252471/139‑1 (MQ=255)

     aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACg                                                                                                                                      <  7:265885/139‑1 (MQ=255)

          attaaaCACTCCAAAGTAAACAATTTAAGTACCATTTCTTAAGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                                 <  4:59567/136‑1 (MQ=255)

             aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                              <  2:419716/139‑1 (MQ=255)

              aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                              <  1:393949/138‑1 (MQ=255)

                  cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                         <  1:91311/139‑1 (MQ=255)

                  cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                         <  1:340515/139‑1 (MQ=255)

                  cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGTTATTTTAACCTTACTAAAGGGaa                                                                                                                         >  1:15692/1‑139 (MQ=255)

                  cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGAAAGTTACACCGTACTAAAGGGaa                                                                                                                         >  4:100349/1‑139 (MQ=255)

                   gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAAt                                                                                                                        <  1:424738/139‑1 (MQ=255)

                   gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAAt                                                                                                                        <  7:377131/139‑1 (MQ=255)

                   gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTTTAATTTTAACGTTAATaaatcggtg                                                                                                                        >  8:392715/1‑133 (MQ=255)

                        aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                                   <  3:221644/139‑1 (MQ=255)

                         gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                                  <  7:127218/139‑1 (MQ=255)

                          tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTGTTTTTTATTTTTTTATTTAAACGTTTCTAAAAGTAATTgatag                                                                                                                  >  6:244309/1‑135 (MQ=255)

                              cAATTTAATTACCATTTCTTTTGATCTAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAATTTAAAAGTTACTAAAGGGAATGGAGATAAatt                                                                                                             <  6:388808/139‑1 (MQ=255)

                                 tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCTCTTTTTTAAATTTTGAAATTTTAAAGTTACTAAAAGGAATTGAGATAAattatt                                                                                                          >  8:42685/1‑139 (MQ=255)

                                 tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                          <  6:209793/139‑1 (MQ=255)

                                 tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                          <  5:64519/139‑1 (MQ=255)

                                 tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                          <  1:301500/139‑1 (MQ=255)

                                 tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                          <  1:6593/139‑1 (MQ=255)

                                 tttAAGTACCATTACTTATGAGCAAGTAATTTCTATTTTTAATAGTTATCTATTATTTCACGTGAGGAAATAATTGTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTATAGGGAATGGAGATAAattatt                                                                                                          <  7:51333/139‑1 (MQ=255)

                                   tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAAttatta                                                                                                         <  3:384627/138‑1 (MQ=255)

                                    aactaacATTACTTTTGAGCCAGTAATGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAg                                                                                                        <  3:326693/132‑1 (MQ=255)

                                    aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                        <  6:151649/138‑1 (MQ=255)

                                    aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                        <  4:131592/138‑1 (MQ=255)

                                    aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                        <  5:361109/138‑1 (MQ=255)

                                    aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCCTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                        <  8:110457/138‑1 (MQ=255)

                                      gTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                     <  6:346714/139‑1 (MQ=255)

                                      gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                     <  8:16398/139‑1 (MQ=255)

                                      gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                                     <  7:240200/139‑1 (MQ=255)

                                       tACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGatat                                                                                                    <  6:313440/139‑1 (MQ=255)

                                         ccATTACTTAAGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                   <  3:194553/138‑1 (MQ=255)

                                           aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGAAAATTTATCTTTAAAAAATGGAATGGAGATAAAATTTTAGTtataat                                                                                                 >  2:241394/1‑136 (MQ=255)

                                           aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTTGTAAGTTAAAAGTTAATAAATGGAATTGAGATAAATTATTTtatataaa                                                                                                 >  8:258997/1‑136 (MQ=255)

                                                atATGAGCAACTAGTGTCTATTTTTAATAGTTATCTATTCTTTACCGGGCGGAAATAATTCTATGAGGCGCTTTTTTAAATTTGGAAAGTTACACGATACTAAAGGGAATGGAGATAAATTATTAGATCTACTACTGAc                                                                                           <  2:69649/138‑1 (MQ=255)

                                                  aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                           <  2:196276/137‑1 (MQ=255)

                                                    gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAATTTTGTAAGTTTAACCTTTATAAAAGGAATTGATTTAAATTTTTATATATAAAACTTACACCt                                                                                       >  2:313910/1‑139 (MQ=255)

                                                        aaGTATTGTCTATTTTTAATTGTTATCTATTATTTAACGGGAGGATATAATTCTTAGTGTCGCTTTTTTAAAATTGGAAAGTTACACGTTTCTATAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                   <  7:409709/139‑1 (MQ=255)

                                                        aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACTGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                   <  8:28974/139‑1 (MQ=255)

                                                        aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                   <  4:29207/139‑1 (MQ=255)

                                                        aaGTAATGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGACAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                   <  6:281582/139‑1 (MQ=255)

                                                         aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                  <  5:16037/139‑1 (MQ=255)

                                                           tATTGTCTATTTTTAATTGTTATCTATTATTTAACGGGAGGAAATAATTCTATGTGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                <  6:421793/139‑1 (MQ=255)

                                                           tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                <  4:387669/139‑1 (MQ=255)

                                                            aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGGTTTTTATTTTTGTTATTTTAACGGTTATTAAGGGAATTGTGATTAATTATTATATATAATAATTACACCTTCCaaga                                                                                >  2:122576/1‑138 (MQ=255)

                                                             ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                              <  7:292587/139‑1 (MQ=255)

                                                                     ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGAAAGTTACACGTTAATAAATGGAATGGAGATAAATTATTATATATAATAATGACAGCTTCCAAGAAGCTAAagag                                                                      >  7:346897/1‑139 (MQ=255)

                                                                      tttAATAGTTATATATTATTTAACTGGAGGAAATATTTCTATGAGTAGCTTTTTTAATTTTGGAAAGTTACATTTTACTATAGGTAATTTATATTAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAtgag                                                                      <  2:350445/138‑1 (MQ=255)

                                                                       ttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTTTAAATTTAAAAGGTTGTAAATGGAATGGAGATAAATTATTATATTTAATAATGACAGCATACAAGAAGCTAAATAGGt                                                                    >  8:137206/1‑139 (MQ=255)

                                                                             gTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                              <  8:229384/139‑1 (MQ=255)

                                                                              ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTGTTTTTAATTTTGAAAGTTTCACGTTACTAAAGGGTATGGTGATAAATTTTTTTATATTATACTGACAGCTTCAAAGAAGCTAAAGAGGTCCCTAgc                                                             >  3:352248/1‑139 (MQ=255)

                                                                               tATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAgc                                                             <  7:83120/138‑1 (MQ=255)

                                                                               tATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTAAATTTGGTAATTTAAAAGGTTAAAAAAGGAAAGGAGATAAATTATTATATATAATAATTAACTCGTCCAAGAAACTAAAGAGGTaccaaacc                                                            >  8:326324/1‑134 (MQ=255)

                                                                                    attattTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTAAAAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTa                                                        <  5:90339/138‑1 (MQ=255)

                                                                                           aaCGGGAGGAAATAATTCTATGAGTCGCGGTTTTTAATTTTGTAAGTTTCACGTTTATAAATGGTTTGGGGATAAATTTTTTTATATACGACTGACAGCGTCCAAGAAGCGAAAGAGGTGCCGTGCGCCTTCGGGGGAt                                                >  4:321219/1‑139 (MQ=255)

                                                                                                  ggaAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggt                                         <  4:414406‑M1/139‑5 (MQ=255)

                                                                                                  ggaAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAAGTTAACCCTTAATAAATGGAATGGATATAAATTATTTTATATAAAAAAGAAACCTGACAATAACCGAAACAGGTCACTAGCGCCTAACGGGACttttgggg                                         >  8:71179/1‑134 (MQ=255)

                                                                                                     aataatTCTATGAGTCTCTTTTTTAAATTTGTAAAGTTACACGTTACTAAATGGAATGTAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                      <  2:383156‑M1/139‑8 (MQ=255)

                                                                                                     aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                      <  1:188749‑M1/139‑8 (MQ=255)

                                                                                                     aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtaca                                      <  2:275611‑M1/139‑8 (MQ=255)

                                                                                                       taatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGTAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                    <  4:16145‑M1/139‑10 (MQ=255)

                                                                                                         atTCTATGAGTAGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                                  <  5:398007‑M1/139‑12 (MQ=255)

                                                                                                            cTATGAGTCGCTTTTTTAAATTTTGTAAGGTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                <  7:319381‑M1/138‑14 (MQ=255)

                                                                                                            cTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                <  3:303808‑M1/138‑14 (MQ=255)

                                                                                                               tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGTGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGACGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                            <  7:188683‑M1/139‑18 (MQ=255)

                                                                                                               tGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                            <  7:214782‑M1/139‑18 (MQ=255)

                                                                                                               tGAGTCGATTTTTTAAATTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTATAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                            <  3:140014‑M1/139‑18 (MQ=255)

                                                                                                                gagtatcttttttATATTTGGAAAGTTTAACTTTAATAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGATTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaagg                            <  5:329911‑M1/132‑18 (MQ=255)

                                                                                                                gAGTCTCTTTTTTAAATTTTGAAAGTTACACGTTAGTTTAGTGTATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGTAATTTGgggtacattgaaaaagg                            <  3:10526‑M1/138‑18 (MQ=255)

                                                                                                                gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTTAAGGGAATGGAGATAAATTATTAGATATACTACTGACCGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                            <  3:407270‑M1/138‑18 (MQ=255)

                                                                                                                gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGTAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                            <  1:268228‑M1/138‑18 (MQ=255)

                                                                                                                gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                            <  5:300539‑M1/138‑18 (MQ=255)

                                                                                                                gAGTAGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGATTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgtgtacattgaaaaagg                            <  5:211948‑M1/138‑18 (MQ=255)

                                                                                                                 aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                          <  2:15228‑M1/139‑20 (MQ=255)

                                                                                                                  gtagattttttaaattTTGAAAGTTAAAATTTACTAAATGTAATGGATATAAATTATTAGATATAATACTGACATCTTCAAAGAATATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                          <  1:133650‑M1/133‑20 (MQ=255)

                                                                                                                  gTCTCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAATAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgtgtacattgaaaaaggaa                          <  8:197001‑M1/138‑20 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                    <  5:187299‑M1/137‑26 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                    <  7:159502‑M1/137‑26 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAAATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                    <  1:363453‑M1/137‑26 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTAATAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                    <  6:180736‑M1/137‑26 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATAATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                    <  6:123640‑M1/137‑26 (MQ=255)

                                                                                                                          tttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                  <  4:229032‑M1/138‑28 (MQ=255)

                                                                                                                             aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta               <  3:46943‑M1/138‑31 (MQ=255)

                                                                                                                              aaTTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt             <  5:4247‑M1/139‑33 (MQ=255)

                                                                                                                              aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt             <  1:231666‑M1/139‑33 (MQ=255)

                                                                                                                                   ggAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                            <  3:166919‑M1/119‑18 (MQ=255)

                                                                                                                                         ttACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccg  <  1:100342‑M1/139‑44 (MQ=255)

TCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACAT  >  Exported/3610‑3885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`