BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	4,625	A→C	6.5%	K252T (AAG→ACG)	ampR →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	4,625	0	A	C	6.5%	15503.1 / 176.8	5700	K252T (AAG→ACG)	ampR	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (3157/2002); new base C (43/457); total (3209/2491)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5801 total aligned reads displayed.

TAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  Exported/4487‑4763

   aCTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  5:380445/1‑139 (MQ=255)

   aCTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  3:220011/1‑139 (MQ=255)

    cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  4:159664/1‑139 (MQ=255)

    cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  6:361957/1‑139 (MQ=255)

    cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  2:107545/1‑139 (MQ=255)

    cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  1:81509/1‑139 (MQ=255)

      ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCGGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCGCTc                                                                                                                                      >  8:70509/1‑139 (MQ=255)

      ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGTGCCAGATGTTAAGCCCTc                                                                                                                                      >  1:360323/1‑139 (MQ=255)

      ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  1:300711/139‑1 (MQ=255)

      ggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  4:514/139‑1 (MQ=255)

       gatggatgCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGGCCTTCCGGGTGGCTTGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCATCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  7:263776/138‑1 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTcc                                                                                                                                     <  4:384186/139‑1 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTcc                                                                                                                                     >  2:370392/1‑139 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTcc                                                                                                                                     >  2:404277/1‑139 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  4:26951/138‑1 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  2:79350/138‑1 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  7:147471/138‑1 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  7:370629/138‑1 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  3:400722/138‑1 (MQ=255)

         tggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCg                                                                                                                                   <  2:13501/139‑1 (MQ=255)

           gaGGCGGATAAAGTAGCAGGACCACTTCTGCGCTCGGCCCTTCCTGCTGGCTGGTTTATTGCGGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTATGCCCTCCCGTa                                                                                                                                 <  5:290622/139‑1 (MQ=255)

              gCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  1:329257/1‑128 (MQ=255)

              gCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              <  4:347436/139‑1 (MQ=255)

               cGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              >  5:255665/1‑138 (MQ=255)

                ggATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            <  6:189288/139‑1 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAt                                                                                                                                <  7:312900/134‑1 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAg                                                                                                                           >  3:70713/1‑139 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAg                                                                                                                           >  5:50060/1‑139 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCATCCCGTATCGTAg                                                                                                                           >  1:174769/1‑139 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGGATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCAGTATCGTAg                                                                                                                           >  1:24795/1‑139 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGGGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  8:372553/128‑1 (MQ=255)

                   tAAAGTTGCCGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                         <  7:237790/139‑1 (MQ=255)

                   tAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGCGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                         <  3:30136/139‑1 (MQ=255)

                     taGTTGGAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATGTGGAGCGGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCGGTATCGTAGTTAt                                                                                                                       <  2:242199/138‑1 (MQ=255)

                          gCAGGACCAGTGCGGCGCGCGGCCCTTCGGGGGGGAGGGTTTATTGCTTATAATGCTGGCGCGGGTGCGGGTGGGTCTCGCGGTAGATTTGCCCCACGGGGGCCAGTTGGTACGCCCGCCCTTATCGTAGTTATCTaca                                                                                                                  <  8:252932/139‑1 (MQ=255)

                           cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  7:96554/1‑139 (MQ=255)

                           cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  4:83308/1‑139 (MQ=255)

                                  cctgcTGCGCGCGCCCCTGCCGGCGGGCGGTTTTACTGCTGATAAATCTGGAGCCGGGGCGGGGGGGCCTCGCGGTATCATTCCAGCCCTGGGGCCTGAGGGAAAGCCCACCCGTATCGTAGTTATCACCACGACgggg                                                                                                          <  2:302006/135‑1 (MQ=255)

                                  aCTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  2:27405/1‑111 (MQ=255)

                                   cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACT‑GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAg                                                                                                        >  7:40657/1‑139 (MQ=255)

                                   cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTACTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                         >  4:190314/1‑123 (MQ=255)

                                         cgcgcgGCCCTGCGGGCTGGGTGGTTTATTTCTCAAAACTGGGCCCCCGGTGCGGGTGGCTCGCGCGGTATCATGGCCCCACGGGGCCCACAGGGAAAGCCCCCCCGTCTCGTAGTTAACAACACGACGGGGAGTCAgg                                                                                                   <  6:331933/139‑1 (MQ=255)

                                         cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCTTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                   >  7:18083/1‑139 (MQ=255)

                                         cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                   >  6:39368/1‑139 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           <  3:100271/94‑1 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  6:46768/1‑96 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  7:345034/1‑99 (MQ=255)

                                                   ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           >  7:44949/1‑89 (MQ=255)

                                                   ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGa                                                                                         >  4:221152/1‑139 (MQ=255)

                                                      ggggtggcggtTTTATGGCTGATAAATCTGGCGCCGGGGCGGGGGGGTCTCGGGGTATCATTGCCGCCCTGGGCCCAGATGGTAAGCCCTCCCTTATCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  8:128343/135‑1 (MQ=255)

                                                      cGGGTGGGTGGTTTTTTGCTTATATATGGGGCGGGGGTGTGGGTGGGTCGCGGGTTATCATTGCAGCAGGGGGGCCTGATGTTAAGCCCTCCGTTATCTTTTTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  8:155311/139‑1 (MQ=255)

                                                        gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAc                                                                                    >  6:82022/1‑139 (MQ=255)

                                                        gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAc                                                                                    >  2:209464/1‑139 (MQ=255)

                                                          tggctggTTTATTGCTGATAAATCTGGCGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAGGGTAAGCCCTCCCGTATCGTAGTTATCTCCACGACGGGGAGTCCGGCCACTATGGCTGACCg                                                                                   >  6:137181/1‑138 (MQ=255)

                                                             ctggTTTATGGCTTATAAATCGGGCGCGGGTGAGCGTGGGTCTCGGGGTATCTTTGCAGCAGTGGGGCCAGATGGTAAGCCCGCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAt                                                                               <  7:117228/139‑1 (MQ=255)

                                                                          gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  1:420447/1‑138 (MQ=255)

                                                                           aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTCTCATTGCAGCCCTGGGGCCTGATGGTAAGCCCTCCCGTATCGTAGTTATCTACCCGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  6:106939/1‑137 (MQ=255)

                                                                           aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  1:272706/1‑137 (MQ=255)

                                                                           aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   >  3:33959/1‑137 (MQ=255)

                                                                           aTAAATCTGGAGCCGGGGAGGGGGGGGCTCGCGGAAACATTGCACCACTGGGGCCAGATGGTAAGCCCGCCCTTAGCCTAGTTATCCACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   <  8:278528/137‑1 (MQ=255)

                                                                                     aGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGcc                                                       >  3:371931/1‑139 (MQ=255)

                                                                                       ccGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCt                                                      >  8:195889/1‑138 (MQ=255)

                                                                                        cGGTGAGGGGGGGCCGCGCTGTATTATTGCAGCTCGGGGGCCAGATGGTACCCCCTCCCTTATCTTATTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  7:28651/139‑1 (MQ=255)

                                                                                          gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  1:217459/1‑139 (MQ=255)

                                                                                          gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  3:197969/1‑139 (MQ=255)

                                                                                          gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                  >  4:414310/1‑139 (MQ=255)

                                                                                               cGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                              >  3:99687/1‑138 (MQ=255)

                                                                                                  gggccgcgcgGTACCATTGCCCCCCGGGGGCCAGAGGGAACGCCCTCCCGTATCGTCTTAATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGc                                          <  6:165853/133‑1 (MQ=255)

                                                                                                    gTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTGATCTACACGACGGGGAGGCAGGCAACTAGGGATGAACGAAATAGACATATCGCTGAGATAGTTGCCTCCCAGATTAAGAAt                                        >  5:17397/1‑139 (MQ=255)

                                                                                                        cgcgGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTgg                                     >  5:197132/1‑138 (MQ=255)

                                                                                                         gcgGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                    >  5:112970/1‑138 (MQ=255)

                                                                                                          cgGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            >  5:7779/1‑49 (MQ=255)

                                                                                                                   tGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            <  3:167186/40‑1 (MQ=255)

                                                                                                                    gcagcaCGGGGGCCAGATGTAAAGCCCTCCCGTAGCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                        <  8:51583/139‑1 (MQ=255)

                                                                                                                     ccgcaCTGGGGCCAGATGGTAAGCCCTCCCGATGCGTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                        <  8:221513/136‑1 (MQ=255)

                                                                                                                     cagccCGGGGGCCAGTTGTAAAGCCCTCCCTTATCGTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                        <  5:354614/138‑1 (MQ=255)

                                                                                                                      cgccgtGGGGCAAGATGTTAAGCCCGCCCGTATCGAAGTAAACAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCaa                      <  8:412102/134‑1 (MQ=255)

                                                                                                                       gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGATCGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                     >  2:170139/1‑139 (MQ=255)

                                                                                                                       gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                     >  5:321727/1‑139 (MQ=255)

                                                                                                                       gaaCTGGGGCCAGAGGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCTCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGCCCAAg                     >  2:335377/3‑139 (MQ=255)

                                                                                                                          cTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                   >  7:67902/1‑138 (MQ=255)

                                                                                                                           tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGttt                  >  3:135376/1‑138 (MQ=255)

                                                                                                                           tGGGCCCAGCTGGTAGCCCCGCCGGCATCGTCTTATTCTCCACTTCGGGGAGTCAGCCAACTAAGGATGAACGAACTAGACAGCTCGCTGAGATACGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtttt                 <  6:29947/139‑2 (MQ=255)

                                                                                                                           gggggCCTTATGGTAAGCCCTCCCGTATCGTAGTTCTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  5:409797/138‑1 (MQ=255)

                                                                                                                           gggggCCTGTGGGTAAGCCCCCCCGTATGGTTTTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  7:209148/138‑1 (MQ=255)

                                                                                                                           gggggCCCGTTGGAACGCCCACCCTTTTCGTAGTTATACCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  5:223439/138‑1 (MQ=255)

                                                                                                                           ggggcCCAGATGGTACGCCCTCCCGTATCGTTGTAATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  1:58544/138‑1 (MQ=255)

                                                                                                                             gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAc                >  3:424846/1‑138 (MQ=255)

                                                                                                                             gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  3:259999/1‑139 (MQ=255)

                                                                                                                             gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  6:256941/1‑139 (MQ=255)

                                                                                                                              ggCCAGAGGGAACCCCCGCCCGTAGCGTAGTACTCACCACGACGGGGAGGCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTc              <  8:342840/139‑1 (MQ=255)

                                                                                                                              gcccAGATGGAAAGCCCGCCCGTCTCTTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTc              <  1:391295/137‑1 (MQ=255)

                                                                                                                                  aGATGGTACGCCGTCCCGTATCGTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat          <  4:130958/139‑1 (MQ=255)

                                                                                                                                  aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata           >  1:101105/1‑138 (MQ=255)

                                                                                                                                   gATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGCATGAACGAAATAGACAGATAGCTGAGAGAGGTGCCTCACAGAGTAAGCATTGGTAACTGTAAGACCAAGTTTACTCatat          >  8:307040/1‑138 (MQ=255)

                                                                                                                                    aTGGTAAGCCCTCCCGTATCGTTGTTATCTACACGACGGGGAGTCAGGCACCTATGGATGAACGAAATAGACAGCTCGCTGAGATAGGTGCCTCCCTGATTAAGCATTGGTAACTGTCAGACAAGGTTTACTCatata         >  1:132365/1‑138 (MQ=255)

                                                                                                                                      ggTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATAc      >  4:306780/1‑139 (MQ=255)

                                                                                                                                         aaGCCCTCCCGTATCTTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt    <  5:145471/138‑1 (MQ=255)

                                                                                                                                         aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 >  2:51696/1‑125 (MQ=255)

                                                                                                                                         aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt    >  6:218201/1‑138 (MQ=255)

TAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  Exported/4487‑4763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`